Biochemical changes in susceptible and resistant black gram cultivars induced by root-knot Nematode, Meloidogyne incognita

Author(s):  
D. K. Nayak ◽  
Ritu Kumari Pandey ◽  
Robert Lepcha ◽  
Rajesh Kumar Kar

Biochemical changes in black gram varieties inoculated with root-knot nematode, Meloidogyne incognita were investigated. Observations were recorded in the biochemical modifications relating to various parameters like total chlorophyll , total sugar contents, protein, and proline content during post infection periods. The variation in total chlorophyll, total protein, proline and total sugar content in six cultivars i.e PU 09-36(S), MU-44(S) ,VBG 11-031(R) ,VBG 11-016(R) ,KUG- 715 (R)and NUL- 205(R) were studied 45 days after inoculation . Reduced percentage of total chlorophyll contents were observed in inoculated samples than the healthy counterparts. However, an increase in amount of total protein ,proline and total sugar contents was observed in the diseased tissues.

Author(s):  
Sameer Kumar Singh ◽  
P. S. Singh

Background: Legume pod borer or spotted pod borer, Maruca vitrata is one of the serious insect pest to the green gram, which causes damage mainly at the reproductive phase of the crop growth. The larvae of spotted pod borer are known to cause damage by webbing the leaves, bud, flower and pods together and feed from inside on them. Due to its webbing nature, it is very difficult to enter inside by natural enemies and chemicals cannot directly reach inside webbing. Host plant resistance to insect pests is an economically and ecologically preferred alternative as compared to other pest management tactics, particularly the synthetic pesticides. The present investigation was undertaken to examine the role of certain bio-chemical constituents of the immature pods of green gram in the expression of damage by the spotted pod borer. Methods: The experiments on screening were conducted during the kharif 2014 and 2015 at the Agricultural Research Farm, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi, (U.P.) to define the occurrence of M. vitrata on 20 promising varieties/genotypes of green gram. Pods were collected at the immature stage from different varieties/genotypes and biochemical constituents such as total soluble sugar, total chlorophyll, protein were estimated. Result: The maximum protein content was found in genotype IPM 306-6 (36.17 mg/g) and minimum in genotype PM-5 (20.53 mg/g). Highest total sugar content was reported from genotype ML 1256 (16.71 mg/g) and lowest total sugar content was recorded in PM-5 (10.94 mg/g).The phenol content in genotype PM-5 (9.00 mg/g) was significantly higher than others whereas, the minimum phenol content was reported in IPM 306-6 (5.14 mg/g). The higher amount of total chlorophyll content found in genotype IPM 306-6 (3.22 mg/g) and lowest in PM-5 (1.85 mg/g). The significant and positive correlation was observed between M. vitrata larval population, pod damage and protein (r=0.954** and r=0.952**, respectively) and total sugar content (r=0.986** and r=0.986**, respectively) and total chlorophyll content (r=0.994** and r=0.993**, respectively) in immature pods, while, the significant and negative correlation was found between phenol (r= -0.916** and r= -0.919**, respectively).


1993 ◽  
Vol 1 (2) ◽  
pp. 99-108 ◽  
Author(s):  
P. Robert ◽  
M.F. Devaux ◽  
A. Qannari ◽  
M. Safar

Multivariate data treatments were applied to mid and near infrared spectra of glucose, fructose and sucrose solutions in order to specify near infrared frequencies that characterise each carbohydrate. As a first step, the mid and near infrared regions were separately studied by performing Principal Component Analyses. While glucose, fructose and sucrose could be clearly identified on the similarity maps derived from the mid infrared spectra, only the total sugar content of the solutions was observed when using the near infrared region. Characteristic wavelengths of the total sugar content were found at 2118, 2270 and 2324 nm. In a second step, the mid and near infrared regions were jointly studied by a Canonical Correlation Analysis. As the assignments of frequencies are generally well known in the mid infrared region, it should be useful to study the relationships between the two infrared regions. Thus, the canonical patterns obtained from the near infrared spectra revealed wavelengths that characterised each carbohydrate. The OH and CH combination bands were observed at: 2088 and 2332 nm for glucose, 2134 and 2252 nm for fructose, 2058 and 2278 nm for sucrose. Although a precise assignment of the near infrared bands to chemical groups within the molecules was not possible, the present work showed that near infrared spectra of carbohydrates presented specific features.


2013 ◽  
Vol 25 (16) ◽  
pp. 9421-9422 ◽  
Author(s):  
G. Peng ◽  
E.V. Davis ◽  
L.X. Wang ◽  
C.W. Zhang

2014 ◽  
Vol 57 (4) ◽  
pp. 507-521 ◽  
Author(s):  
Joanna Ostrzycka ◽  
Marcin Horbowicz ◽  
Włodzimierz Dobrzański ◽  
Leszek S. Jankiewicz ◽  
Jan Borkowski

Tomatillo is widely cultivated in Mexico but is little known in other countries. The chemical composition of fruit from field grown plants was investigated during several vegetative seasons. Tomatillo contained a relatively high percentage of dry matter (7-10%) and extract (6.6-7.4%). Its potassium content was lower than that of tomato growing in the same conditions. The content of iron was higher, and that of other elements was comparable, depending on the conditions during the given year. The total sugar content amounted to 2.8-5.7%, depending on the selected population. The percentage of glucose and fructose decreased during ripening and that of saccharose increased. The content of pectic substances was similar as in tomato but the proportions of particular fractions was different. Tomatillo contained more acids than tomato, and showed an especially high citric and malic acid content. The latter decreased drastically during ripening. The content of oxalic acid was 11-18 mg 100 g<sup>-1</sup> in ripe fruit and up to 54 mg in unripe. The vitamin C content depended on the selected population and amounted to 8-21 mg 100 g<sup>-1</sup>, dehydroascorbic acid prevailing. The content of vitamin PP was 0.8-1.3 mg 100 g<sup>-1</sup>.


2018 ◽  
Vol 11 (7) ◽  
pp. 1986-1993 ◽  
Author(s):  
André Machado Rech ◽  
Fábio Henrique Weiler ◽  
Marco Flôres Ferrão

1968 ◽  
Vol 23 (5) ◽  
pp. 671-675 ◽  
Author(s):  
G. Drews

The thylakoids (chromatophores) of the sulfur-free purple bacteria Rhodospirillum rubrum contain 30% lipids soluble in methanol-chloroform, 46% protein, and 14% carbohydrates. 90% of the total sugar content was glucose, 4% fucose, 5% rhamnose. In the thylakoids of Rhodopseudomonas viridis 3,3% sugar was demonstrable (50% glucose, 17% galactose, 15% rhamnose and 17% mannose). 2-keto-3-desoxy-octonate is a structure component in both organisms. After treatment of thylakoids with phenol/water, the main sugar fraction was in the water phase, although in both organisms 3 to 4% of the protein fraction in the phenol phase consists of sugar.


1961 ◽  
Vol 1 (3) ◽  
pp. 153 ◽  
Author(s):  
JF Melvin ◽  
MA Sutherland

The effect of shading for 64 hours before cutting on the soluble sugar contents of short rotation ryegrass has been determined at weekly intervals during growth. From grass with five to six leaves emergent to the stage of growth usually accepted for hay-making, the average reduction in the total sugar content of the shaded plants was about 30 per cent, made up of decreases in sucrose (12 per cent), oligosaccharides (5 per cent) and fructosans (13 per cent).


2010 ◽  
pp. 81-86
Author(s):  
O. Franco-Mora ◽  
O. Pérez-Huerta ◽  
E.J. Morales-Rosales ◽  
A. González-Huerta ◽  
M. Huerta-Lara

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