Characterization and validation of point mutation in exon 19 of CACNA2D1 gene in Karan Fries (Bos taurus x Bos indicus) cattle

The objective of this study was to characterize and validate the candidate point mutation in Calcium channel, voltage-dependent, alpha-2/ delta subunit 1 (CACNA2D1) gene in Karan Fries (Bos taurus x Bos indicus) cattle. The CACNA2D1 gene reported as one of the potential candidate gene influencing Somatic cell Score and Mastitis. A PCR product of 249 bp amplifying the exon 19 and partial 18 and 19 intronic region of CACNA2D1 gene was digested with Hae III restriction enzyme to screen the reported point mutation. A monomorphic banding pattern with genotype AA was found in Karan Fries cattle. Sequencing was also carried out to characterize and explore insilico screened mutation in the nucleotide sequence of a particular region. The result indicates highly conserved sequence in Karan Fries cattle. The Phylogenetic tree revealed that Karan Fries cattle were closer to Bos taurus cattle, Bos mutus (Yak), and Bison bison (American buffalo) compared to other species.

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DGAT1 polymorphism in Bos indicus and Bos taurus cattle breeds

Journal of Dairy Research ◽

10.1017/s0022029904000032 ◽

2004 ◽

Vol 71 (2) ◽

pp. 182-187 ◽

Cited By ~ 67

Author(s):

Bernhard Kaupe ◽

Andreas Winter ◽

Ruedi Fries ◽

Georg Erhardt

Keyword(s):

Milk Fat ◽

Bos Taurus ◽

Bos Indicus ◽

Milk Composition ◽

Major Effect ◽

Potential Candidate ◽

Fat Percentage ◽

Cattle Breeds ◽

Potential Candidate Gene ◽

Trait Locus

As a result of multiple QTL-mapping projects in recent years, a quantitative trait locus for milk fat percentage and milk yield has been described on BTA14. Recent reports name the acyl-CoA[ratio ]diacylglycerol acyltransferase (DGAT1) gene on BTA14 as a potential candidate gene, with a nonconservative substitution of lysine by alanine (K232A) producing a major effect on milk composition and yield. DGAT1K appears to be the ancestral allele and the K232A substitution probably occurred after the divergence of the Bos indicus and Bos taurus lineages. These findings prompted us to genotype 1748 DNA samples of 38 different Bos taurus and Bos indicus cattle breeds from 13 countries on five continents (Europe, Africa, Asia, North America and South America), to examine the occurrence of the DGAT1 polymorphism and characterize the K232A substitution in cattle breeds of different origins and selected for different purposes (e.g., beef, dairy and dual purpose). Calculating pairwise FST values for pooled subpopulations showed least divergence for Bos indicus breeds with high milk fat percentage. Fixation of DGAT1A was found in some Bos taurus breeds and fixation of DGAT1K in one Bos indicus breed. Breeds of no known organized breeding background from the Near East domestication centre of Bos taurus and taurine African N'Dama cattle were found to possess intermediate frequencies of DGAT1K. While beef breeds tended to harbour higher DGAT1A levels, dairy cattle showed everything from very low levels of DGAT1K to unexpectedly high frequencies of this allele.

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Sequence characterization and polymorphism detection in lactoferrin gene of Deoni (Bos indicus) cattle

Indian Journal of Animal Research ◽

10.18805/ijar.6711 ◽

2015 ◽

Author(s):

Arun Pratap Singh ◽

K. P. Ramesha ◽

S. Isloor ◽

P. Divya ◽

Ashwani Arya ◽

...

Keyword(s):

Bos Indicus ◽

Point Mutations ◽

Single Strand Conformation Polymorphism ◽

Potential Candidate ◽

Coding Region ◽

Cattle Breeding ◽

Sequence Characterization ◽

Potential Candidate Gene ◽

Dairy Cattle Breeding ◽

Lactoferrin Gene

Lactoferrin is an iron binding glycoprotein which plays an essential role in antimicrobial defense and a potential candidate gene in dairy cattle breeding. The present study was undertaken with the objective of sequence characterization and identification of polymorphisms within exons 2, 3 and 14 and their flanking intronic regions in lactoferrin gene of Deoni (Bos indicus) cattle by single strand conformation polymorphism (SSCP). Amplicons of exons 4, 5 and 16 showed monomorphic patterns. PCR-SSCP analysis revealed a total of eight different variants in three investigated regions of the lactoferrin gene. The locus LtfE2 revealed four different variants, viz. LtfE2-A, LtfE2-B, LtfE2-C and LtfE2-D with the frequency of 0.42, 0.26, 0.22 and 0.10, respectively. Analysis of exon 3 and 14 revealed two unique SSCP patterns with the frequencies of 0.54 and 0.46, 0.86 and 0.14 respectively. Comparison of nucleotide sequences of lactoferrin gene of the Deoni cattle with taurine cattle revealed a total of 12 point mutations, 11 of which were found to be in coding region with 10 transitions. Conceptualized translation of nucleotide sequence revealed 5 amino acid changes. The SNPs identified in the coding region of lactoferrin gene may serve as potential genetic marker (s) in cattle for disease resistance.

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Molecular characterization and SNP identification in HSPB6 gene in Karan Fries (Bos taurus x Bos indicus) cattle

Tropical Animal Health and Production ◽

10.1007/s11250-017-1235-6 ◽

2017 ◽

Vol 49 (5) ◽

pp. 1059-1063

Author(s):

Rakesh Kumar ◽

I. D. Gupta ◽

Archana Verma ◽

Ragini Kumari ◽

Nishant Verma

Keyword(s):

Molecular Characterization ◽

Bos Taurus ◽

Bos Indicus ◽

Karan Fries

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Genetic polymorphism in HSPB6 gene and their association with heat tolerance traits in Indian Karan Fries (Bos taurus x Bos indicus) cattle

Animal Biotechnology ◽

10.1080/10495398.2021.1899939 ◽

2021 ◽

pp. 1-12

Author(s):

Rakesh Kumar ◽

Ishwar Dayal Gupta ◽

Archana Verma ◽

Sohanvir Singh ◽

Ragini Kumari ◽

...

Keyword(s):

Genetic Polymorphism ◽

Heat Tolerance ◽

Bos Taurus ◽

Bos Indicus ◽

Karan Fries

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Seasonal Change in Oxidative Stress Markers in Blood Plasma of Tharparkar (Bos indicus) and Karan Fries (Bos indicus x Bos taurus) Cattle under Tropical Climate

International Journal of Current Microbiology and Applied Sciences ◽

10.20546/ijcmas.2017.602.192 ◽

2017 ◽

Vol 6 (2) ◽

pp. 1720-1730 ◽

Cited By ~ 2

Author(s):

Uttarani Maibam ◽

Om K. Hooda ◽

Phurailatpam S. Sharma ◽

Sohan V. Singh ◽

Ramesh C. Upadhyay

Keyword(s):

Oxidative Stress ◽

Blood Plasma ◽

Seasonal Change ◽

Bos Taurus ◽

Bos Indicus ◽

Tropical Climate ◽

Oxidative Stress Markers ◽

Stress Markers ◽

Karan Fries ◽

In Blood Plasma

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Sequence Characterization of Forebrain Embryonic Zinc Finger-like (FEZL) Gene in Indian Zebu (Bos indicus) Cattle and their Crossbreds

Indian Journal of Animal Research ◽

10.18805/ijar.b-4166 ◽

2021 ◽

Author(s):

S. Rajesh Kumar ◽

I.D. Gupta ◽

S. Goyal ◽

Kathiravan Periasamy ◽

A. Verma ◽

...

Keyword(s):

Zinc Finger ◽

Neuronal Development ◽

Bos Taurus ◽

Bos Indicus ◽

Complete Sequence ◽

Gene Markers ◽

Sequence Characterization ◽

Exon 1 ◽

Karan Fries

Background: Forebrain embryonic zinc finger-like (FEZL) gene is an important candidate associated with mastitis resistance in dairy cattle. FEZL is involved in transcriptional regulation of neuronal development and there exists a crosstalk between neuronal development and immunity via downstream cytokine expression. A single glycine insertion into glycine stretch of FEZL gene has large effect on downstream cytokine pathway making the cows susceptible to mastitis. The present study was aimed to sequence characterize FEZL gene in Sahiwal (Bos indicus) and Karan Fries (Bos inidcus X Bos taurus) cattle.Methods: Sequence characterization of bovine FEZL gene was carried out by primer walking method. Ten sets of oligonucleotide primers were designed to synthesize overlapping fragments and generate the complete sequence of about 3.7 kb covering all exons and 5’ upstream regulatory and flanking regions.Result: A total of eight nucleotide variations including three INDELS and five substitution mutations were observed among FEZL gene sequences of Bos taurus, Bos indicus (Sahiwal) and Bos taurus X Bos indicus (Karan Fries) cattle. The conceptualized amino acid sequence of bovine FEZL gene in Sahiwal and Karan Fries cattle was found to have 13 tandem Glycine residues and a serine to proline change within exon 1 region. The percent identity of FEZL gene of Sahiwal and Karan Fries cattle was 99% with that of Bos taurus, 95% with dog, horse and pig, 94% with human, 93% with rabbit, 92% with marmoset, 89% with rat and 79% with chicken. Sequence characterization of ~0.7 kb 5’ flanking region showed that it is highly conserved among bovines and resulted in prediction of six putative sites for binding of transcription factors (including Elk-1, Oct-1, HNF4, Lmo2 complex, GATA-3 and Nkx2-5). Elucidation of Bos indicus FEZL gene will further form the basis to identify candidate gene markers for association with mastitis resistance/susceptibility in cattle.

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Major histocompatibility complex (DRB3) geneexpression pattern indicates differences in BrucellaabortusS19 vaccine induced immune responsein Karan Fries and Sahiwal cattle

Indian Journal of Animal Research ◽

10.18805/ijar.b-3577 ◽

2018 ◽

Author(s):

D. Ravi Kumar ◽

S. K. Mishra ◽

Anshuman Kumar ◽

Vineeth M.R. S. Jayakumar ◽

A. Sakthivel Selvan ◽

...

Keyword(s):

Genetic Basis ◽

Mononuclear Cells ◽

Bos Taurus ◽

Bos Indicus ◽

Strong Response ◽

Peripheral Blood Mononuclear ◽

Genetic Groups ◽

Histocompatibility Complex ◽

Antigenic Reactivity ◽

Karan Fries

Brucella abortus S19 strain vaccination is most effectively used as a tool to control the brucellosis in cattle. To understand the genetic basis of differences in immune responsiveness after immunization in cattle of different genotypes, we assessedthe expression of MHC-DRB3 antigen receptor molecule in six each female calves of Karan Fries crossbreds (KF, Bos indicus x Bos taurus) and Sahiwal (Bos indicus) vaccinated with Brucella S19. Serum and peripheral blood mononuclear cells (PBMC) were isolated from blood collected on 0(before vaccination) and 7, 14 and 28 day of vaccination. Antigenic response was assessed for these days in both the groups using Rose Bengal Plate form Test (RBPT). At 0d, the calves of both groups showed no antigen agglutination, confirming the calves free from the infection.The serum of 7d onward started showing the agglutination with more strong response in later stages specifically in KF, indicating increased immune response against Brucella. Therefore, RBPT can be used as earliest screening (7d onward) for Brucella antigenic reactivity in both cattle groups. The expression of DRB3 gene started with slight upregulation after vaccination,in general, however without any significant differences between two different genetic groups upto14d.The significant (p Lass Than 0.01) higher expression (8 times) of DRB3was observed in KF than Sahiwal at 28d. The study indicated that antigenic reactivity and MHC-DRB3 expression elicited by Brucella S19 vaccination was more prominent in KFduring initial days, which may provide an extra advantage to the host for antigen binding, thereby better immune protection at later stage.

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Novel SNP identification in exon 3 of HSP90AA1 gene and their association with heat tolerance traits in Karan Fries (Bos taurus × Bos indicus) cows under tropical climatic condition

Tropical Animal Health and Production ◽

10.1007/s11250-016-1016-7 ◽

2016 ◽

Vol 48 (4) ◽

pp. 735-740 ◽

Cited By ~ 5

Author(s):

Rakesh Kumar ◽

Ishwar Dayal Gupta ◽

Archana Verma ◽

Sohan Vir Singh ◽

Nishant Verma ◽

...

Keyword(s):

Heat Tolerance ◽

Climatic Condition ◽

Bos Taurus ◽

Bos Indicus ◽

Karan Fries ◽

Novel Snp

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Genome-wide identification of cyclin-dependent kinase (CDK) genes affecting adipocyte differentiation in cattle

BMC Genomics ◽

10.1186/s12864-021-07653-8 ◽

2021 ◽

Vol 22 (1) ◽

Author(s):

Cuili Pan ◽

Zhaoxiong Lei ◽

Shuzhe Wang ◽

Xingping Wang ◽

Dawei Wei ◽

...

Keyword(s):

Gene Family ◽

Expression Analysis ◽

Adipocyte Differentiation ◽

Bos Taurus ◽

Adipogenic Differentiation ◽

Critical Role ◽

Bos Indicus ◽

Specific Expression ◽

Genome Wide ◽

A Genome

Abstract Background Cyclin-dependent kinases (CDKs) are protein kinases regulating important cellular processes such as cell cycle and transcription. Many CDK genes also play a critical role during adipogenic differentiation, but the role of CDK gene family in regulating bovine adipocyte differentiation has not been studied. Therefore, the present study aims to characterize the CDK gene family in bovine and study their expression pattern during adipocyte differentiation. Results We performed a genome-wide analysis and identified a number of CDK genes in several bovine species. The CDK genes were classified into 8 subfamilies through phylogenetic analysis. We found that 25 bovine CDK genes were distributed in 16 different chromosomes. Collinearity analysis revealed that the CDK gene family in Bos taurus is homologous with Bos indicus, Hybrid-Bos taurus, Hybrid Bos indicus, Bos grunniens and Bubalus bubalis. Several CDK genes had higher expression levels in preadipocytes than in differentiated adipocytes, as shown by RNA-seq analysis and qPCR, suggesting a role in the growth of emerging lipid droplets. Conclusion In this research, 185 CDK genes were identified and grouped into eight distinct clades in Bovidae, showing extensively homology. Global expression analysis of different bovine tissues and specific expression analysis during adipocytes differentiation revealed CDK4, CDK7, CDK8, CDK9 and CDK14 may be involved in bovine adipocyte differentiation. The results provide a basis for further study to determine the roles of CDK gene family in regulating adipocyte differentiation, which is beneficial for beef quality improvement.

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