Comparative analysis of semen quality parameters and their relationship with fertility in different genetic groups of layer chicken

2018 ◽  
Author(s):  
Gurjot Kaur Mavi ◽  
P.P. Dubey ◽  
Ranjna S. Cheema ◽  
S. K. Dash ◽  
B. K. Bansal
Keyword(s):  
Rhode Island ◽  
Semen Quality ◽  
Sperm Concentration ◽  
Quality Parameters ◽  
Fertility Rate ◽  
Maintenance Cost ◽  
Genetic Groups ◽  
Layer Chicken ◽  
Significant Difference ◽  
Sperm Traits

The present study was conducted to characterize the semen quality parameters in roosters of three genetic groups viz. Rhode Island Red (RIR), Punjab Red and RIR X local cross. The roosters for each genetic group were also evaluated for fertility rate. There was no significant difference in color, pH and abnormalities among different genetic groups. RIR X local cross had significant higher (p less than 0.01) ejaculate volume (0.51±0.02 ml vs. 0.27±0.01 and 0.30±0.01ml), sperm concentration (2804.70±114.61 vs. 1826.10±77.66 and 2133.50±81.18X106 sperms/ml), sperm motility % (66.60±2.12 vs. 55.38±1.82 and 51.95±1.84), sperm viability % (72.43±2.06 vs. 64.67±1.88 and 51.25±1.89) and HOST % (64.59±2.61 vs. 58.55±1.17 and 47.09±2.11) than RIR and Punjab Red respectively. Interestingly, RIR X local cross had significant lower (p less than 0.01) partially damaged sperm % (36.96±2.81 vs. 41.84±1.78 and 48.98±1.89) and fully damaged sperm % (14.15±3.39 vs. 26.27±1.93 and 31.25±2.46) than RIR and Punjab Red respectively indicating desirable performance. The fertility rate for RIR X local cross was also found to be higher than Punjab Red and RIR respectively. Sperm traits were significantly positively correlated among groups with high correlation between fertility rate and sperm traits. It can be concluded that the selection of roosters on the basis of sperm function tests for artificial insemination may improve fertility rate and thereby may reduce maintenance cost of large number of cocks.

Effect of age on semen quality in naked neck and dwarf chicken under tropical climatic conditions

2012 ◽  
Vol 52 (10) ◽  
pp. 964 ◽  
Author(s):  
M. Shanmugam ◽  
U. Rajkumar ◽  
M. R. Reddy ◽  
S. V. Rama Rao
Keyword(s):  
Semen Quality ◽  
Sperm Concentration ◽  
Quality Parameters ◽  
Climatic Conditions ◽  
Naked Neck ◽  
Significant Difference ◽  
Dye Reduction ◽  
Effect Of Age ◽  
Dwarf Chicken

The present study was undertaken to elucidate the effect of age on semen quality of naked neck and dwarf chicken lines under tropical climatic conditions. Semen collected by abdominal massage at 4-week intervals from 24 to 48 weeks of age was evaluated for different semen quality parameters. Except sperm concentration and appearance, all other parameters were significantly (P < 0.05) affected by the age of the birds. The semen volume, motility, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide dye reduction test and percentage of live sperm were significantly (P < 0.05) lower at 24 weeks of age compared with 40 weeks of age. At 24 weeks of age the dead sperm percentage was significantly (P < 0.05) high and with advancing age it decreased. There was a significant difference (P < 0.05) between the gene lines for volume, appearance, motility, sperm concentration, and percentage of live and dead sperm. Except for sperm concentration, significant (P < 0.05) interaction between gene lines and age was observed in other parameters. In conclusion, semen quality was different between naked neck and dwarf line males and age affected semen quality of the gene lines studied in different manners.

scholarly journals Comparison of semen characteristics between indigenous and Amo breeds cockerel of Gombe State, Nigeria

2019 ◽  
Vol 15 (2-1) ◽  
pp. 303-306
Author(s):  
Tijjani Haruna Usman ◽  
Saleh Mohammed Sir ◽  
Ma’aruf Bashir Sani
Keyword(s):  
Semen Quality ◽  
Sperm Concentration ◽  
Progressive Motility ◽  
Indigenous Breed ◽  
Normal Sperm ◽  
Abnormal Sperm ◽  
Significant Difference ◽  
Semen Characteristics ◽  
Genetically Improved ◽  
Abdominal Massage

The experiment was carried out to compare the semen characteristics of indigenous and Amo strains of cockerel at poultry unit of teaching and research farm of Federal University of Kashere, Gombe State, Nigeria. Semen samples were collected from nine indigenous and nine Amo breeds of cockerel at three days interval for two weeks using abdominal massage technique. Semen samples were examined macroscopically for semen colour, pH and ejaculation volume. Then, microscopic observation was carried for sperm concentration, mass motility, progressive motility, live and dead sperms percentage, normal and abnormal sperm, all for semen characteristics. The results showed a significant difference (P ≤ 0.05) between mass motility, progressive motility, sperm concentration and head defects of 4.85 ± 0.27 to 4.37 ± 0.19, 95.13 ± 0.43 to 81.63 ± 1.15%, 4.93 ± 1.84 to 3.40 ± 1.07×109/ml and 2.96 ± 0.17 to 3.44 ± 0.12% for indigenous and Amo breeds of cockerel, respectively. There were no significant differences observed as semen colour, ejaculate volume, semen pH, live / dead normal sperm neck (mid-piece), tail defects and sperm total abnormalities were found to be 2.85 ± 0.07 to 2.00 ± 0.090.21 ± 0.17 to 0.20 ± 0.02 /ml, 88.85 ± 0.58 to 72.70 ± 0.54% /ml, 11.14 ± 0.58 to 27.29 ± 0.54%, 81.00 ± 0.78 to 66.22 ± 0.61%,9.03 ± 0.42 to 13.96 ± 0.47%, 9.70 ±  to 13.00 ± 0.30 and 21.70 ± 0.59 to 30.40 ± 0.53% for the indigenous and Amo breed groups of cockerel, respectively. It was concluded that semen quality characteristics could be differed between genetically improved (Amo strain) and indigenous breed of cockerels.

scholarly journals Variation of Semen Quality Among Three Generations (F1, F2 and F3) of Holstein Friesian Upgraded Breeding Bulls of Bangladesh

2020 ◽  
Vol 7 (3) ◽  
pp. 457-463
Author(s):  
Faruk Hossain ◽  
Md Golam Sorowar ◽  
Sharmin Akter Suma ◽  
Abdullah Al Mansur ◽  
Md Mahbubul Hoque ◽  
...  
Keyword(s):  
First Generation ◽  
Semen Quality ◽  
Sperm Concentration ◽  
Quality Parameters ◽  
Quality Parameter ◽  
Microsoft Excel ◽  
Three Generations ◽  
Holstein Friesian ◽  
Recorded Data ◽  
Mass Activity

The experiment was conducted in a well-known private livestock research and development farm in Bangladesh to find out the variation of semen quality parameter among three generations (F1, F2 and F3) of Holstein Friesian upgraded breeding bulls. A total of 312 ejaculates were collected from 6 upgraded breeding bullsthrought the experimental year. The recorded data were summarized using Microsoft Excel 2010 and analyzed using GraphPad Prism 5 softwere. Out of the 312 ejaculates, 273 (87.50%) were found to be creamy in color followed by 26 (8.33%) and 13 (4.17%) as yellowish and watery, respectively. Generation had significant (P<0.05) effect on ejaculate volume, consistancy, mass activity, sperm concentration, initial and post freezing motility. The highest (7.389±0.19ml) and the lowest (5.156±0.13ml) volume of semen were found in third (F3) and first generation (F1), respectively. The mass activity ranges from 3.74±0.04 to 4.30±0.05. Sperm concentration and pH varied insignificantly (p>0.05) but initial motility and post freezing motility had the significant differences among the three generations. Initial motility ranges from 75.87±0.32 to 78.40±0.38 percent and the post freezing motility ranges from 50.38±0.41 to 52.16±0.43 percent. It could be concluded that most of the semen quality parameters were influenced by generation and freezing. Semen characteristics were better in F2 followed by F3 and F1 generation in upgraded Holstein Friesian bulls. Res. Agric., Livest. Fish.7(3): 457-463,  December 2020

scholarly journals Effect of Age and Frequency of Collection on Quality of Jersey Bulls Semen at National Livestock Breeding Center (NLBC), Nepal

2019 ◽  
Vol 7 (1) ◽  
pp. 88-95 ◽  
Author(s):  
S. Sankhi ◽  
K.R. Sapkota ◽  
B. Regmi
Keyword(s):  
Semen Quality ◽  
Sperm Concentration ◽  
Quality Parameters ◽  
Bull Semen ◽  
Livestock Breeding ◽  
Abnormal Sperm ◽  
Breeding Center ◽  
Live Sperm ◽  
Level Of Significance ◽  
Effect Of Age

Objective: To determine the effect of age and frequency of collection on quality parameters of Jersey bull semen at National Livestock Breeding Center (NLBC), Pokhara. Materials and Methodology: Nine Jersey bulls were selected randomly from NLBC. To obtain the effect of age, six bulls of three different age interval (3-4, 5-7 and 8-9 years) were selected randomly. Remaining three bulls were used to determine the effect of frequency of collection on semen quality. Studies were conducted for three months (Nov/Dec to Dec/Jan, 2016). Semen quality was analyzed using CASA system and SMILE software. ANOVA and paired t- tests were applied to find out level of significance. All the statistical analyses were done using SPSS 17.0 Results: Sperm concentration, post- thaw motility, live sperm, and abnormal sperm were significantly affected by the age of the bulls. Semen quality was found superior in the bulls belonging to 5-7 years of age intervals. Significantly higher value for semen volume, sperm concentration and post- thaw motility was obtained on second collection (P<0.05). However, volume initial motility, pre-filling motility, live sperm and abnormal sperm percentage did not differ significantly among different age intervals and between the frequencies of collection. Conclusion: Different quality parameters of semen like volume, concentration, initial motility, pre-filling motility, post thaw motility, live and normal spermatozoa percentages were higher in 5-7 years old bulls and in second collection of semen. Int. J. Appl. Sci. Biotechnol. Vol 7(1): 88-95

scholarly journals Association between semen quality among men with different occupational exposures and risk of recurrent spontaneous abortion in island residents

2019 ◽  
Vol 02 (02) ◽  
pp. 49-57
Author(s):  
Wenli Cao ◽  
Xinyao Pan ◽  
Feijun Ye ◽  
Jing Zhou ◽  
Zengshu Huang ◽  
...  
Keyword(s):  
Spontaneous Abortion ◽  
Semen Quality ◽  
Sperm Concentration ◽  
Occupational Exposures ◽  
Recurrent Spontaneous Abortion ◽  
Semen Parameters ◽  
Control Group ◽  
Effective Prevention ◽  
Mechanistic Investigation ◽  
Significant Difference

Objective: This study aimed to evaluate the semen quality of male adults whose spouses suffer from recurrent spontaneous abortion (RSA) in the island area, and to explore the association between RSA and occupational exposures. Methods: A total of 131 male patients were recruited and divided into two groups: spouse with recurrent spontaneous abortion group (RSA group, [Formula: see text]) and the normal fertility group (control group, [Formula: see text]). Information such as height, weight and occupational exposure history of 131 men were obtained. Semen samples were collected and analyzed. Differences in semen parameters and DNA fragmentation index (DFI) between the two groups were compared. Odds ratios (ORs) and their corresponding 95% confidence intervals were calculated to evaluate the association between occupational exposures and RSA status. Results: Overall, no significant difference was found in sperm concentration, progressive motility and normal morphology rate between RSA and control groups. Only DFI was observed to be significantly higher in the RSA group. Evaluation of the receiver operating characteristic (ROC) curve showed DFI (AUC: 0.623, [Formula: see text]) could discriminate between males from the couples with RSA and without RSA. Conclusion: The analysis of conventional semen parameters could not directly reflect their influence on embryonic development, and test of integrity of the sperm DNA is of paramount importance to fully understand male fertility. It is crucial to conduct studies regarding occupational exposures and pregnancy loss and/or RSA risk, since it will provide population-level data to aid in the identification of important risk factors that warrant further mechanistic investigation, and eventually lead to effective prevention and treatment strategies.

scholarly journals Effect of Age and Frequency of Collection in Quality of Jersey Bulls Semen at National Livestock Breeding Center, Nepal

2018 ◽  
Vol 35 ◽  
pp. 9-22
Author(s):  
S. Sankhi ◽  
K.R. Sapkota ◽  
B. Regmi
Keyword(s):  
Semen Quality ◽  
Sperm Concentration ◽  
Quality Parameters ◽  
Bull Semen ◽  
Livestock Breeding ◽  
Abnormal Sperm ◽  
Breeding Center ◽  
Live Sperm ◽  
Level Of Significance ◽  
Effect Of Age

The present study was undertaken to determine the effect of age and frequency of collection on quality parameters of Jersey bull semen at National Livestock Breeding Center (NLBC), Pokhara, Kaski district. Nine Jersey bulls were selected randomly from NLBC. To obtain the effect of age, six bulls of three different age interval (3- 4, 5-7 and 8-9 years) were selected randomly. Remaining three bulls were used to determine the effect of frequency of collection on semen quality. Studies were conducted for three months (Nov/Dec to Dec/Jan 2017). Semen quality was analyzed using CASA system and SMILE software. ANOVA and paired t- tests were applied to find out level of significance. All the statistical analyses were done using SPSS 17.0. Sperm concentration (0.551 ±0.004 to 1.084 ± 0.014x109), post- thaw motility (42.44±0.11 to 51.72±0.03 %), live sperm (45.62 ± 0.13 to 51.23±0.15 %), and abnormal sperm (11.57±0.12 to 19.59±0.24%) were significantly affected by the age of the bulls. Semen quality was found superior in the bulls belonging to 5-7 years of age intervals. On the other hand, volume (3.98±0.09 to 6.72±0.06 ml), initial motility (72.68±0.02 to 78.64±0.15 %) and pre-filling motility (71.34±0.17 to 77.62±0.02 %) did not differ significantly among different age intervals. Significantly, higher value for semen volume (3.98±0.12 to 6.02±0.14 ml), sperm concentration (0.572±0.119 to 1.095±0.015) and post- thaw motility (40.34±0.21 to 48.54±0.06% was obtained on second collection. However, initial motility (70.58±0.06 to 75.64±0.13 %), prefilling motility (68.11±0.12 to 71.62±0.02 %), live sperm (53.27±0.54 to 56.17±0.02 %) and abnormal sperm (12.31±0.12 to 14.3±0.47 %) did not differ significantly between the frequencies of collection. Different quality parameters of semen like volume, concentration, initial motility, pre-filling motility, post thaw motility, live and normal spermatozoa percentage were higher in 5-7 years old bulls and in second collection of semen.

scholarly journals Comparison of Serum Inhibin B and Follicle-Stimulating Hormone (FSH) Level between Normal and Infertile Men in Yaoundé

2020 ◽  
Vol 2020 ◽  
pp. 1-9 ◽  
Author(s):  
Tchoula Mamiafo Corinne ◽  
Pieme Constant Anatole ◽  
Ngogang Yonkeu Jeanne
Keyword(s):  
Semen Quality ◽  
Follicle Stimulating Hormone ◽  
Sperm Concentration ◽  
Reproductive Hormones ◽  
Inhibin B ◽  
World Health ◽  
Infertile Couple ◽  
Male Reproductive Function ◽  
Significant Difference ◽  
Stimulating Hormone

Objective. Hormones play a vital role in initiating and maintaining male reproductive function. The present study explores the influence and predictive ability of two reproductive hormones on semen quality among men who were partners in an infertile couple. Design. During our cross sectional study, men were recruited from private and public hospital and laboratories for clinical evaluation of fertility status. Methods. Fresh semen samples were assessed for quality (concentration, motility and morphology) according to the 2010 World Health Organization manual and the serum levels of hormones, including follicle-stimulating hormone (FSH), Inhibin B was measured (ELISA). Results. We found a significant difference in the two groups regarding sperm concentration (p<0.0001), total sperm count (p<0.0001), progressive motility (p<0.0001), vitality (p<0.0001) and the percentage of normal forms (p=0.043). We found a strong and negative correlation between FSH and Inhibin B in the overall population, the normozoospermic reference group and the case group. Our study confirmed that Inhibin B was significantly and positively correlated with sperm concentration and leucocytes, and that FSH was negatively correlated to sperm concentration and vitality. Conclusion. Consistent with other studies, our results show strong association between semen quality and FSH and Inhibin B.

Effect of Dietary Copper and Zinc Supplementation on Semen Quality of Murrah Bulls

2020 ◽  
Author(s):  
Abhradip Majumder ◽  
Manjula Thakur ◽  
Mukesh Bhakat ◽  
Manorama Saha ◽  
Tushar Kumar Mohanty ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Seminal Plasma ◽  
Semen Quality ◽  
Sperm Concentration ◽  
Basal Diet ◽  
Sod Activity ◽  
Significant Difference ◽  
T1 And T2 ◽  
Cu And Zn ◽  
In Blood Plasma

The effect of dietary supplementation of Cu and Zn on semen quality parameters and certain bio-chemicals parameters were evaluated in Murrah bulls. Twelve mature Murrah bulls (4-6 years of age) were divided into three groups (n=4) T1, T2, and T3 based on semen volume and concentration and were fed as per ICAR standard (2013). However, the animals were supplemented with 0%, 25%, and 50% Cu and Zn above the basal diet in T1, T2 and T3 groups, respectively for 180 days. Quantitative and qualitative characteristics of semen, blood and seminal plasma antioxidant status, blood and semen minerals (Cu, Zn, Ca and Mn) were determined in experimental Murrah bulls. Semen ejaculate volume (mL) increased in T2 and T3 while sperm concentration (million/mL), intact acrosome (%), HOST reacted spermatozoa (%) increased in T3 group compared to T1 and T2 groups. No difference was observed in mass motility, pH, live spermatozoa (%) in semen sexual behaviour except dismounting time which was decreased in T3 than T1 and T2 (plessthan0.05). SOD activity in blood plasma and LPO activity decreased in seminal plasma in both T2 and T3 groups than T1, whereas catalase activity did not show any significant difference. Cu and Zn supplementation in T2 group improved plessthan0.05) Zn level in blood and seminal plasma and Cu level in blood plasma only, but not in seminal plasma. Therefore, it can be concluded that supplementation of Zn and Cu at 50% above the recommended levels of ICAR (2013) improved the qualitative and quantitative attributes of semen in Murrah bulls.

scholarly journals Effect of genotype on semen quality traits of main and reciprocal crossbred chickens

2020 ◽  
Vol 46 (2) ◽  
pp. 32-39
Author(s):  
U. C. Isaac ◽  
U. H. Udoh ◽  
R. J. Nosike
Keyword(s):  
Reciprocal Cross ◽  
Semen Quality ◽  
Sperm Concentration ◽  
Genetic Constitution ◽  
Quality Traits ◽  
Breeding Method ◽  
Naked Neck ◽  
Significant Difference ◽  
Live Sperm

Semen quality of cocks is of utmost importance in reproduction. Breeding method, breed, strain and genetic constitution are the major components of the genetic factors capable of affecting the semen quality of cocks. This research seeks to determine the effect of genotype on the semen quality traits of 272 F crossbred cocks produced at day-old by main and 1 reciprocal crossbreeding of 69 Isa Brown and local frizzle feathered, naked neck and normal feathered chickens. The genotypes of the cocks were Isa Brow x frizzle feathered main cross (IBxF), Isa Brown x naked neck main cross (IBxNa), Isa Brown x normal feathered main cross (IBxN), frizzle feathered x Isa Brown reciprocal cross (FxIB), naked neck x Isa Brown reciprocal cross (NaxIB) and normal feathered x Isa Brown reciprocal cross (NxIB). At 36-40 weeks of age, semen was extracted from the cocks by abdominal  massage technique and used to evaluate semen volume (SV), sperm motility (SM), sperm concentration (SC) and live sperm (LS) at weekly intervals. Data obtained from the evaluation were subjected to analysisof variance and tested at 5% level of probability. Genotypes differed significantly (P <0.05) in SC at 37-40 weeks and in SM and LS at all ages. There was no significant difference (P>0.05) in SV. Regardless of genotype and age, the mean SV, SM, SC and LS ranged from 0.31±0.02 to 0.24±0.01ml, 71.84±1.33 to 58.75±2.16 %, 3.22±0.02 to2.92±0.02 x109/ml and 71.91±1.37 %, respectively. The NxIB and IBxNa genotypes produced largest numericalsemen volume at 37 and 39 weeks of age respectively. Whereas SC and LS were significantly highest for IBxF and IBxN respectively at week 37, SM was so for IBxN at all ages. Further, the LS of IBxN genotype was significantly highest at 36-38 and 40 weeks of age. The exotic Isa Brown and normal feathered main cross cocks exhibited the best semen quality traits among all genotypes studied and should be selected for improvement of the reproductive characteristics of cocks.  

scholarly journals 294EFFECTS OF LONG DAYLIGHT ON CAPRINE SCROTAL CIRCUMFERENCE AND SEMEN QUALITY

2004 ◽  
Vol 16 (2) ◽  
pp. 267
Author(s):  
A. Mahmoud ◽  
B. Kouakou ◽  
E. Amoah ◽  
S. Gelaye
Keyword(s):  
Repeated Measures ◽  
Semen Quality ◽  
Sperm Concentration ◽  
Small Ruminants ◽  
Reproductive Activity ◽  
Least Square ◽  
Semen Parameters ◽  
Nonbreeding Season ◽  
Scrotal Circumference ◽  
Significant Difference

Seasonal variation of the reproductive activity of small ruminants in the southern USA is controlled by photoperiod. We tested the hypothesis that artificial manipulation of photoperiod has an effect on caprine testicular activity and semen quality. The objective of this study was thus to determine the effects of photoperiod on caprine scrotal circumference and semen parameters during the nonbreeding season. Ten Saanen and Alpine bucks, two to three years old, raised at the Small Ruminant Research and Extension Center of Fort Valley State University (latitude 32°37′ N, longitude 83°39′ W), were used in the study. Throughout the study, the bucks were fed a concentrate diet containing 16% crude protein, and 2.7 Mcal/Kg of digestible energy. Two groups of bucks randomly selected were exposed to long daylight (16L:8D; n=5) or ambient daylight (12L:12D, control;; n=5) for a period of three weeks during the month of March. Scrotal circumference measurements and semen collection were conducted on each buck twice a week for a total of five trials. Semen samples were collected using an artificial vagina and an electroejaculator. Samples were immediately transferred to the laboratory for further evaluation. Data were analyzed as a completely randomized design with repeated measures using SAS program (SAS, 1990). When means were significant by ANOVA (P&lt;0.05), they were separated by least significant difference. Results are presented as the least square means±SEM. Long daylight did not affect semen volume (mL), % motility, sperm concentration (billions/mL), % viability, or % abnormality(P&gt;0.05). However, semen motility, sperm concentration, and abnormality were influenced by treatment in trials 3, 1, and 5, respectively (P&lt;0.05). The least square means±SEM values for these parameters were 35±15, 0.58±0.1, and 20.0±3.7 for long daylight-treated bucks v. 87±15, 0.13±0.1, and 7.8±3.7 for the control, respectively. Further, long daylight affected scrotal circumference (P&lt;0.05). Scrotal circumferences were decreased in trials 1, 2, and 5 (P&lt;0.05). Long daylight decreased scrotal circumference without affecting semen quality in this study.

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