Ultrastructural Study of the Outer Cortex, Deep Cortex and Medulla of Mesenteric Lymph Node in Adult Goats

2020 ◽  
Author(s):  
K. Balasundaram ◽  
S. Sivagnanam
Keyword(s):  
Lymph Node ◽  
T Lymphocytes ◽  
Lymph Nodes ◽  
B Lymphocytes ◽  
Mesenteric Lymph Node ◽  
Outer Cortex ◽  
Mesenteric Lymph ◽  
Cortical Parenchyma ◽  
Reticular Cells ◽  
Medullary Parenchyma

Background: A mesenteric lymph node in the sub pyloric region which is the longest among all mesenteric lymph nodes in adult goats was identified for study under the scanning electron microscope. Methods: The outer cortical, deep cortical and medullary parenchyma of the collected lymph nodes was thoroughly screened under VEGA3 TESCAN. The outer cortical parenchyma revealed T lymphocytes, B lymphocytes, Sinus Lining Cells (SLC) and Sinus Reticular Cells (SRC) at a magnification of 9600x. The deep cortical parenchyma revealed Reticular cells, T lymphocytes and Interdigitating cells (IDC) at 8000x magnification.Result: The medullary parenchyma revealed T lymphocytes, B lymphocytes, Sinus Lining Cells (SLC), Fibrous Reticular Cells (FRC) and medullary sinuses at a magnification of 8500x. Plasma cells and lymphocytes also remained in the sinus. The morphology and relations of the cells observed were recorded.

scholarly journals Immunocytochemistry of mesenteric lymph node fine-needle aspirates in the diagnosis of feline infectious peritonitis

2019 ◽  
Vol 31 (2) ◽  
pp. 210-216 ◽  
Author(s):  
Sandra Felten ◽  
Katrin Hartmann ◽  
Stefanie Doerfelt ◽  
Laura Sangl ◽  
Johannes Hirschberger ◽  
...  
Keyword(s):  
Lymph Node ◽  
Lymph Nodes ◽  
Mesenteric Lymph Node ◽  
Complex Method ◽  
Mesenteric Lymph Nodes ◽  
Predictive Values ◽  
Feline Infectious Peritonitis ◽  
Fine Needle ◽  
Fine Needle Aspirates ◽  
Mesenteric Lymph

Immunohistochemistry (IHC) of tissue samples is considered the gold standard for diagnosing feline infectious peritonitis (FIP), and, in cats without body cavity effusion, IHC is the only method available to establish definitive antemortem diagnosis. However, IHC requires invasive tissue sample collection. We evaluated sensitivity and specificity of an immunocytochemical assay of fine-needle aspirates (FNAs) of mesenteric lymph nodes that can be obtained noninvasively by ultrasound-guided aspiration to diagnose FIP. FNAs of mesenteric lymph nodes were obtained postmortem from 41 cats suspected of having FIP based on clinical and/or laboratory findings. FIP was confirmed immunohistochemically in 30 cats. In the other 11 cats, a disease other than FIP, which explained the clinical signs, was diagnosed histopathologically. Immunocytochemistry (ICC) was performed as an avidin–biotin complex method using a monoclonal anti-FCoV IgG 2A. Sensitivity, specificity, negative and positive predictive values (NPV, PPV, respectively) including 95% confidence intervals (95% CIs) were determined. ICC was positive in 17 of 30 cats with FIP, but also in 1 of 11 control cats that was diagnosed with lymphoma. Sensitivity of ICC was 53% (95% CI: 34–72); specificity 91% (95% CI: 59–100); NPV 42% (95% CI: 22–63); and PPV 94% (95% CI: 71–100). In a lethal disease such as FIP, specificity is most important in order to avoid euthanasia of unaffected cats. Given that a false-positive result occurred and FIP was correctly detected in only approximately half of the cases of FIP, ICC of mesenteric lymph node FNA alone cannot reliably confirm or exclude FIP, but can be a helpful test in conjunction with other diagnostic measures.

Mesenteric lymphadenopathy in patients with germ cell tumor.

2012 ◽  
Vol 30 (5_suppl) ◽  
pp. 347-347
Author(s):  
Hooman Djaladat ◽  
Craig R. Nichols ◽  
Siamak Daneshmand
Keyword(s):  
Lymph Node ◽  
Lymph Nodes ◽  
Germ Cell ◽  
Germ Cell Tumor ◽  
Mesenteric Lymph Node ◽  
Frozen Section ◽  
Cell Tumor ◽  
High Dose ◽  
Cell Transplant ◽  
Mesenteric Lymph

347 Background: Mesenteric lymphadenopathy may be secondary to inflammatory, infectious or tumoral pathologies. The most common malignancies causing mesenteric adenopathy are gastrointestinal and lymphoma. To the best of our knowledge, there are no reports of germ cell tumor (GCT) presenting with mesenteric adenopathy. Methods: Four patients with GCTs presenting with mesenteric adenopathies were treated in two academic centers (OHSU and USC) by a single surgeon since 2004. All pathologies were non-seminomatous GCT. Their presentation, clinico-pathologic findings and outcome are reviewed. Results: Two cases (19 and 51 yo) were IGCCC poor risk, stage II, testicular GCTs presenting with bulky retroperitoneal, periportal and mesenteric adenopathy. They both underwent post-chemo RPLND with mesenteric lymph node biopsy/resection. The intraoperative mesenteric lymph node frozen section study in one case revealed embryonal cell carcinoma and teratoma; he had early postoperative recurrence and is awaiting high dose chemotherapy and autologous stem cell transplant (HDC/ASCT). The other was teratoma and underwent resection, however he developed lung metastases with elevated AFP 6 months later and was treated with HDC/ASCT, being disease free for 2.5 years. The third case was a 29 yo IGCCC good risk testicular GCT who presented with retroperitoneal (II B) and mesenteric lymphadenopathy. He underwent post-chemo exploration and intraoperative frozen section of the mesenteric lymph nodes showed fibrosis and histiocytic infiltration; therefore classic RPLND was completed. The fourth case was a 24 yo HIV (-) patient with extragonadal GCT originating from the rectosigmoid. At presentation, he had a widespread mesenteric adenopathy, partially responded to primary (BEP) and salvage chemotherapy (VIP); he underwent recto/sigmoid resection, RPLND and PLND and had 52/104 lymph nodes positive for yolk sac tumor. He was referred for HDC/ASCT. Three cases were done recently with limited follow-up. Conclusions: The most common etiologies for mesenteric adenopathy are inflammatory, infection and neoplastic diseases. In the presence of germ cell tumor however, mesenteric adenopathy is most likely secondary to metastasis rather than secondary pathology.

scholarly journals Rates of Proliferation and Interrelationships of Cells in the Mesenteric Lymph Node of the Rat

Blood ◽  
1963 ◽  
Vol 22 (6) ◽  
pp. 674-689 ◽  
Author(s):  
WILLIAM O. RIEKE ◽  
RUTH W. CAFFREY ◽  
N. B. EVERETT
Keyword(s):  
Lymph Node ◽  
Cell Lines ◽  
Mesenteric Lymph Node ◽  
Plasma Cells ◽  
Tritiated Thymidine ◽  
Cell Types ◽  
Tissue Sections ◽  
Mesenteric Lymph ◽  
Reticular Cells ◽  
Proliferating Cell

Abstract Single and multiple injections of tritiated thymidine were combined with radioautography to study the rates of proliferation and interrelationships of the various cell lines in the mesenteric lymph node of the rat. The appearance and labeling patterns of the different cells are described from studies of both smears and tissue sections. Reticular cells exhibit wide variations in labeling intensity, phagocytize labeled lymphocytes, and become labeled in high percentages only when TTH is administered over a period of many days. Other slowly proliferating cell types include small lymphocytes, fat cells, endothelial cells and mast cells. Rapidly proliferating cell lines include plasmablasts, hemohistioblasts, proplasmacytes and large lymphocytes. The generation time of plasmablasts and hemohistioblasts was determined to be approximately 9 and 12 hours respectively. Mature plasma cells constitute a non-dividing population which is renewed in lymph node in not more than 5 days. Evidence is presented that the most primitive cells in the lymphocyte and plasma cell lines are the hemohistioblasts and plasmablasts respectively. Reticular cells most probably are not stem cells. No evidence is found to support previous reports that plasma cells derive from lymphocytes.

Morphology of the lymph nodes in bottlenose dolphin (Tursiops truncatus) and striped dolphin (Stenella coeruleoalba) from the Adriatic Sea

2005 ◽  
Vol 53 (1) ◽  
pp. 1-11 ◽  
Author(s):  
Snježana Vuković ◽  
H. Lucić ◽  
H. Gomerčić ◽  
Martina Duras Gomerčić ◽  
T. Gomerčić ◽  
...  
Keyword(s):  
Lymph Node ◽  
Lymph Nodes ◽  
Mesenteric Lymph Node ◽  
Tursiops Truncatus ◽  
Adriatic Sea ◽  
Lymphatic Tissue ◽  
Stenella Coeruleoalba ◽  
Striped Dolphin ◽  
Terrestrial Mammals ◽  
Mesenteric Lymph

Morphology of the lymph nodes was examined in six bottlenose dolphins (Tursiops truncatus) and three striped dolphins (Stenella coeruleoalba) from the Adriatic Sea. All animals had been found dead in nature. One group of the nodes was taken from the tracheal branching area and was marked as bifurcational lymph node, and the other group was taken from the mesenteric root and was marked as mesenteric lymph node. Microscopic analysis showed that the lymph nodes in both dolphin specieswere surrounded by a connective tissue capsule comprising smooth muscle cells. The parenchyma of the mesenteric and bifurcational lymph nodes in bottlenose dolphinwas divided into the peripherally situated cortex with the lymphatic nodules and diffuse lymphatic tissue, and the centrally situated medulla structured of the medullary cords separated by the medullary sinuses. These lymph nodes structurally correspond to the lymph nodes in the majority of terrestrial mammals. The mesenteric lymph node of striped dolphin also had a peripherally situated cortex and a centrally positioned medulla as the majority of terrestrial mammals. In the bifurcational lymph nodes of striped dolphin, there was a central dense lymphatic tissue with the lymphatic nodules and a peripheral less dense lymphatic tissue structured of the cell cords and sinuses. The bifurcational lymph node in striped dolphinresembledporcine lymph nodes and belonged to the inverse lymph nodes.

Immunohistochemical evaluation of cellular composition of the immune system of lymph nodes in acute appendicitis

2019 ◽  
Vol 88 (4) ◽  
pp. 218-226
Author(s):  
Jakub Żurawski ◽  
Patrycja Talarska ◽  
Stanisław Łazowski ◽  
Marcin Grochowalski ◽  
Jacek Karoń
Keyword(s):  
Lymph Node ◽  
Immune System ◽  
T Lymphocytes ◽  
Acute Appendicitis ◽  
Lymph Nodes ◽  
B Lymphocytes ◽  
Mucous Membrane ◽  
Neutrophil Infiltration ◽  
Muscle Membrane

Introduction. There is not much data about the composition of populations of the immune system in acute appendicitis. The basic histopathological criterion for the diagnosis of acute appendicitis is neutrophil infiltration of the muscle membrane. Aim. The subject of this publication is a semi-quantitative evaluation of B lymphocytes (CD20+), T lymphocytes (CD3+) and macrophages (CD68+), and the determination of the number of active lymph nodes during the course of inflammation.Material and Methods. The study material was obtained from 79 patients who had an appendectomy due to acute appendicitis. In this group, the tissue was obtained from: 34 women (aged 20 to 91) and 45 men (aged 20 to 72).Results. In the course of acute appendicitis, there is involvement of lymph node B lymphocytes, T lymphocytes and macrophages. Independent of the type of inflammation, the cellular make-up of the nodes is similar. The number of lymph nodes decreases with age and is gender dependent.Conclusions. In the course of acute appendicitis, there is involvement of lymph node B lymphocytes, T lymphocytes and macrophages. The number of lymph nodes decreases with age and is gender dependent. A statistically significant number of the examined cells of the immunological system in the lymph nodes changed due to inflammation (p<0.001). B and T lymphocytes in the lymph nodes and in the mucous membrane of the appendix differed depending on the sex, and the presence of B lymphocytes in the mucous membrane was significantly higher in the group of 20-40 years of age. T lymphocytes were predominant in the centres of the lymph nodes in groups 20-40 and 61-91 years of age, and in the peripheral zones in the group of 41-60 years of age.

scholarly journals Cavitating Mesenteric Lymph Node Syndrome in Association with Coeliac Disease and Enteropathy Associated T-Cell Lymphoma: A Case Report and Review of the Literature

2010 ◽  
Vol 2010 ◽  
pp. 1-4 ◽  
Author(s):  
Olivia M. B. McBride ◽  
Richard J. E. Skipworth ◽  
Derek Leitch ◽  
Satheesh Yalamarthi
Keyword(s):  
Case Report ◽  
Lymph Node ◽  
T Cell ◽  
Lymph Nodes ◽  
Coeliac Disease ◽  
Mesenteric Lymph Node ◽  
Cell Lymphoma ◽  
Mesenteric Lymph Nodes ◽  
Mesenteric Lymph ◽  
Lymph Node Syndrome

Cavitating mesenteric lymph node syndrome (CMLNS) is a rare and poorly understood complication of coeliac disease (CD), with only 37 cases reported in the literature. CD is an immune-mediated enteropathy, with alterations seen in the small bowel architecture on exposure to ingested gluten. Those who fail to respond to a strict gluten-free diet are termed to have refractory coeliac disease (RCD). This is associated with serious complications such as enteropathy-associated T-cell lymphoma (EATL). We present the case of a 71-year-old female investigated for weight loss and a palpable intraabdominal mass. Abdominal computed tomographic (CT) scan showed multiple necrotic mesenteric lymph nodes. At operation, multiple cavitating mesenteric lymph nodes, containing milky fluid, were found. An incidental EATL was found at the terminal ileum, which was resected. The patient subsequently tested positive for CD. This is the second case report to document an association between CMLNS and EATL. This paper highlights the varied presentation of CD. In this case, the diagnosis of CD was made retrospectively after the complications were dealt with. This paper is followed by a review of relevant literature.

Collision Metastasis of Prostatic and Colonic Adenocarcinoma: Report of 2 Cases

2004 ◽  
Vol 128 (3) ◽  
pp. 318-320 ◽  
Author(s):  
Zane K. Wade ◽  
John E. Shippey ◽  
Gregory A. Hamon ◽  
Robert L. Smoak ◽  
Pedram Argani ◽  
...  
Keyword(s):  
Lymph Node ◽  
Lymph Nodes ◽  
Urothelial Carcinoma ◽  
Malignant Lymphoma ◽  
Mesenteric Lymph Node ◽  
Prostatic Carcinoma ◽  
The Other ◽  
Colonic Adenocarcinoma ◽  
Mesenteric Lymph

Abstract Collision metastases of carcinomas in lymph nodes are rare. Carcinoma metastasizing to lymph nodes containing malignant lymphoma has also been reported. The literature contains 3 cases of collision metastasis of prostatic and urothelial carcinoma. To our knowledge, we report the first 2 cases of collision metastasis of prostatic and colonic adenocarcinoma, one involving a mesenteric lymph node and the other involving a perirectal lymph node, both from resections for colonic adenocarcinoma in patients with known metastatic prostatic carcinoma.

Analysis of T cell populations and IL-3 mRNA expression in mesenteric lymph node cells and intestinal intraepithelial lymphocytes in Strongyloides ratti-infected mice

1998 ◽  
Vol 72 (1) ◽  
pp. 1-8 ◽  
Author(s):  
T. Abe ◽  
H. Sugaya ◽  
K. Yoshimura
Keyword(s):  
Lymph Node ◽  
Lymph Nodes ◽  
Mesenteric Lymph Node ◽  
Intraepithelial Lymphocytes ◽  
Cell Populations ◽  
Mrna Levels ◽  
Mesenteric Lymph Nodes ◽  
Intestinal Intraepithelial Lymphocytes ◽  
Mesenteric Lymph ◽  
Lymph Node Cells

AbstractT cell populations and IL-3 mRNA expression were analysed in mesenteric lymph node cells and intestinal intraepithelial lymphocytes (IEL) in Strongyloides ratti- infected mice. On days 7 and 12 post-infection, 2.6 times as many mesenteric lymph node cells were present in S. ratti- infected mice compared with uninfected mice. Although the percentages of CD3+, CD4+ and CD8+ cells decreased during infection, the absolute numbers of these cell types increased on day 7 due to an overall increase in the mesenteric lymph node cell number. The CD4/CD8 ratio in IEL was increased on day 5, whereas no significant change in the CD4/CD8 ratio was observed in the mesenteric lymph node cells. Expression of IL-3 mRNA, which is an important cytokine for the induction of murine mucosal mastocytosis and S. ratti- expulsion, was examined in mesenteric lymph nodes and IEL of uninfected and infected mice. IL-3 mRNA was detected in mesenteric lymph nodes of S. ratti-infected mice but not detected in the lymph nodes of uninfected mice. IL-3 mRNA was detected in IEL from both infected and uninfected mice with an 20-fold increase in expression in IEL of infected mice. Overall, IL-3 mRNA levels were higher in IEL than in mesenteric lymph nodes following S. ratti- infection. Expression of IL-4, IL-10, stem cell factor (SCF or c-kit ligand) and IFN-γ mRNA was also examined in these two tissues. IL-10 mRNA was not detected in any tissue examined and IFN-γ mRNA levels were unaltered as a result of an S. ratti- infection. Elevated expression of mRNA for SCF (5-fold) and IL-4 (20-fold) was observed in the mesenteric lymph nodes of infected mice. In contrast, SCF mRNA levels were similar in IEL of uninfected and infected animals and only a modest increase in IL-4 mRNA was observed in IEL of infected mice.

scholarly journals High Prevalence and Increased Severity of Pathology of Bovine Tuberculosis in Holsteins Compared to Zebu Breeds under Field Cattle Husbandry in Central Ethiopia

2007 ◽  
Vol 14 (10) ◽  
pp. 1356-1361 ◽  
Author(s):  
Gobena Ameni ◽  
Abraham Aseffa ◽  
Howard Engers ◽  
Douglas Young ◽  
Stephen Gordon ◽  
...  
Keyword(s):  
Lymph Node ◽  
Lymph Nodes ◽  
Mesenteric Lymph Node ◽  
Bovine Tuberculosis ◽  
Control Program ◽  
Tuberculin Test ◽  
Skin Lesions ◽  
Mesenteric Lymph ◽  
Significant Difference ◽  
Cattle Husbandry

ABSTRACT A comparative study on the prevalence and pathology of bovine tuberculosis (TB) was conducted on 5,424 cattle (2,578 zebus, 1,921 crosses, and 925 Holsteins), which were kept on pasture in the central highlands of Ethiopia, using a comparative intradermal tuberculin test, postmortem examination, and bacteriology. The overall prevalence of bovine TB was 13.5%; prevalence was higher in Holsteins than either zebus (22.2% versus 11.6%, χ2 = 61.8; P < 0.001) or crosses (22.2% versus 11.9%, χ2 = 50.7; P < 0.001). Moreover, the severity of pathology in Holsteins (mean ± standard error of the mean [SEM], 6.84 ± 0.79) was significantly higher (P = 0.018) than the severity of pathology in zebus (5.21 ± 0.30). In addition, the risk of TB in Holsteins was more than twice (odds ratio [OR] = 2.32; 95% confidence interval [CI] = 1.89, 2.85) that in zebus. Animals between 5 and 9 years of age were at higher (OR = 2.37; 95% CI = 1.80, 3.12) risk of bovine TB than those 2 years of age or below. A significant difference (χ2 = 351; P < 0.001) in the occurrence of TB lesions in lymph nodes was recorded; the mesenteric lymph node (mean pathology score ± SEM, 1.95 ± 0.08) was most severely affected, followed by the retropharyngeal (0.80 ± 0.05) and caudal mediastinal (0.8 ± 0.06) lymph nodes. Fifty-six percent (n = 145) of the animals with gross TB lesions were culture positive; the lowest culture positivity was recorded in the skin lesions (27.3%) and the lesions of the mesenteric lymph node (31.5%). Both the skin test response and the postmortem findings suggested a higher susceptibility to bovine TB in Holsteins than zebus under identical field husbandry conditions (on pasture). In the light of increased numbers of Holstein cattle introduced into this area to raise milk production to satisfy the needs of Addis Ababa's growing population, these findings highlight the need for a control program in these herds.

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