Genetic Polymorphism in Prolactin Gene and its Effect on Test Day Milk Production Traits in Sahiwal Cattle

2021 ◽  
Author(s):  
T. Karuthadurai ◽  
A.K. Chakravarty ◽  
A. Kumaresan ◽  
D.N. Das ◽  
A. Sakthivel Selvan ◽  
...  
Keyword(s):  
Genetic Polymorphism ◽  
Milk Production ◽  
Milk Yield ◽  
Early Stage ◽  
Direct Sequencing ◽  
Production Performance ◽  
Prolactin Gene ◽  
Pcr Rflp ◽  
Sahiwal Cattle ◽  
Fat Yield

Background: The selection of genetically superior animals at an early stage of life, the molecular markers are used along with traditional selection. The study was carried out to identify the genetic polymorphism in the exon3 region of the Prolactin and enumerate its effect on milk production performance in Sahiwal cattle. Prolactin plays an imperative regulatory role in mammary gland development, milk emission and lactogenesis. Analysed the sequence of this gene to explore whether mutations in this sequence and it could be accountable for quantitative variations in milk production and its composition traits.Methods: Total DNA was isolated from the blood samples of 98 pedigreed Sahiwal population. Using PCR-RFLP method and direct sequencing, noticed a single-nucleotide polymorphism in exon3 region of the Prolactin gene in 156bp and also the effect of non- genetic factors on each trait was assessed by least-squares analysis for non-orthogonal data by a fixed model.Result: PCR-RFLP was done with RsaI restriction endonuclease for the identification of different genotypes. The frequency of G and A alleles of the Prolactin gene was evaluated as 0.575 and 0.425, whereas the frequencies of GG, GA and AA genotypes for the Prolactin gene were 0.45, 0.25 and 0.30, respectively. SNP (G55A) conferred an increase in test-day milk yield around 321.5g, in test day fat yield around 13.9g and in test day SNF yield increase was 19.4g, respectively. High correlation was perceived from test day (TD2) onwards between test day traits and lactation milk yield indicating that selection based on identified SNP in TD2 increased test day milk yield, fat yield and SNF yield by 1.1472 kg, 29.6gm and 45.4gm, respectively.

Identification of genetic marker for prolactin gene related to milk yield in pedigreed Sahiwal population

2018 ◽  
Author(s):  
T. Karuthadurai ◽  
A.K. Chakravarthy ◽  
A. Kumaresan ◽  
Aneet Kour ◽  
B.S. Pradeep Nag ◽  
...  
Keyword(s):  
Regression Analysis ◽  
Restriction Endonuclease ◽  
Genetic Marker ◽  
Milk Production ◽  
Milk Yield ◽  
Prolactin Gene ◽  
Pcr Rflp ◽  
Gene Exon

The study was conducted to identify the genetic marker for prolactin gene related to milk yield in pedigreed Sahiwal population. PCR-RFLP was carried out by using Rsa1 restriction endonuclease and revealed three genotypes GG, GA and AA in Sahiwal population. The frequency of G and A allele of Prolactin gene (exon 3) was estimated as 0.575 and 0.425, whereas the frequencies of GG, GA and AA genotypes for Prolactin gene (exon 3) were estimated as 0.45, 0.25 and 0.30, respectively. Regression analysis inferred that, SNP (G55A) of prolactin gene (exon 3) contributed to an increase in the average test day milk yield by 321.5g which was 3.92% of the average test day milk yield. Forty Sahiwal animals (20 families) under Daughter- Dam Design were screened and it was observed that one SNP (G55A) was found in all the nine families (45%) having heterozygous (GA) genotype. Thus, it could be considered as a potential genetic marker for high milk production in Sahiwal population.

Genetic variability in exon 5 region of GH1 gene and its effect on milk production and milk composition traits in Karan Fries cattle

2018 ◽  
Author(s):  
Aneet Kour ◽  
A. K. Chakravarty ◽  
T. Karuthadurai ◽  
Ekta Rana ◽  
Varinder Raina
Keyword(s):  
Genetic Variability ◽  
Restriction Endonuclease ◽  
Milk Production ◽  
Milk Yield ◽  
Milk Composition ◽  
Production Performance ◽  
Fat Yield ◽  
Karan Fries ◽  
Gene Exon

The study was conducted to identify the genetic variability in exon 5 region of GH1 gene and quantify its effect on production performance in Karan Fries cattle.PCR-RFLP method using Alu I restriction endonuclease was used for identification of genotypes. LL and LVgenotypes with frequencies as 0.46 and 0.54 and the frequency of L allele as 0.73 were found. Season of calving was only found significant (p £0.05) for fat yield of TD3, TD6 and TD10. The effect of SNP of GH1 gene (exon 5) increased the overall test day milk yield, fat yield and SNF yield by 1.05 kg, 43.2 gm and 103 gm.High correlations were obtained from TD3 onwards between test day traits and lactation milk yield indicating that selection based on identified SNP in TD3 increased test day milk yield, fat yield and SNF yield by 2.5229 kg, 62.9 gm and 215.9 gm, respectively.

Effect of non-genetic factors on milk production performance and composition traits in Sahiwal cattle

2020 ◽  
Vol 73 (6) ◽  
pp. 587-591
Author(s):  
Suchit Kumar ◽  
Anupama Mukherjee ◽  
Alok Kumar Yadav ◽  
Prajwalita Pathak ◽  
Saleem Yousuf
Keyword(s):  
Milk Production ◽  
Genetic Factors ◽  
Production Performance ◽  
Sahiwal Cattle

scholarly journals Influence of reproductive qualities on dairy productivity of cows of the Simmental breed

2021 ◽  
Vol 285 ◽  
pp. 04005
Author(s):  
Maria Grinchuk ◽  
Yulia Nesterova
Keyword(s):  
Milk Production ◽  
Milk Yield ◽  
Mass Fraction ◽  
Milk Protein ◽  
Production Performance ◽  
Dry Period ◽  
Milk Productivity ◽  
Service Period ◽  
Positive Effect ◽  
Different Levels

The aim of work is to explore influence of first fruitful insemination, service period and dry period to dairy productivity of Simmental cows. The research is aimed at studying the milk production of Simmental cows with different levels of reproductive qualities. The result of the research, a relation was established between the age of the first fruitful insemination, the duration of the service period, the duration of the dry period and milk production performance. It has been established that the age of the first fruitful insemination at 18-24 months has the most beneficial effect on subsequent milk production. The service period of 90-120 days has a positive effect to an increase in milk yield per 305 days of lactation, the longer service period negatively affects to the mass fraction of fat. With an increase in the duration of the dry period, the mass fraction of fat increases, but milk yield decreases. The influence of reproductive qualities to the milk protein is negligible. It was revealed that the age of the first insemination at 18-24 months is the most profitable for production. According to the results of the research, it can be judged that reproductive qualities, in combination with other factors, affect the milk productivity of Simmental cows.

The use of short-term secretion rate measurements for estimating the milk production of suckler cows

1978 ◽  
Vol 45 (1) ◽  
pp. 1-4 ◽  
Author(s):  
Y. L. P. Le Du ◽  
R. D. Baker ◽  
J. M. Barker
Keyword(s):  
Dairy Cows ◽  
Milk Production ◽  
Milk Yield ◽  
Milk Composition ◽  
Secretion Rate ◽  
Short Term ◽  
Beef Cow ◽  
Total Milk ◽  
Fat Yield ◽  
Good Agreement

SummaryTwo experiments with dairy cows and one with suckler cows and their calves were conducted to examine the use of secretion rate measurements for estimating total milk production. In the first experiment both 4- and 6- h intervals between measurements gave similar estimates of total 7-d milk yield. The second experiment compared estimated and measured milk composition as well as yield. Milk and solids-not-fat yields were underestimated with dairy cows as a result of an extended milking interval before measurement. However, fat yield was overestimated, indicating that all residual milk was not removed at the first oxytocinaided milking. It was concluded that for the beef cow, previous interval effects would be eliminated by the frequency of calf suckling, but that residual milk effects might cause a 3–6% and a 16% overestimation of milk and fat yields respectively.In the third experiment, the milk yield of suckler cows was estimated from measurements of secretion rate and from changes in calf weight; good agreement was obtained provided there were at least 3 consecutive controlled sucklings.

scholarly journals Use of Cold-Pressed Sunflower Cake in the Concentrate as a Low-Input Local Strategy to Modify the Milk Fatty Acid Profile of Dairy Cows

Animals ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. 803 ◽  
Author(s):  
Idoia Goiri ◽  
Izaro Zubiria ◽  
Hanen Benhissi ◽  
Raquel Atxaerandio ◽  
Roberto Ruiz ◽  
...  
Keyword(s):  
Milk Production ◽  
Dry Matter ◽  
Milk Composition ◽  
Production Performance ◽  
Composition Analysis ◽  
Acceptance Test ◽  
Overall Acceptability ◽  
Fat Yield ◽  
Sunflower Cake ◽  
Cold Pressed

Cold-pressed sunflower cake (CPSC) is a cheap by-product of oil-manufacturing. Supplementing diets with CPSC, rich in fat and linoleic acid, could be an effective tool for increasing healthy fatty acids (FA) in milk. To test this hypothesis, 10 cows were used in a crossover design with two experimental diets fed during two 63-day periods. Cows’ milk production was recorded and samples were taken for fat, protein, lactose, and for FA composition analysis. Dry matter intake (DMI) and dry matter apparent digestibility (DMD) were estimated using two markers. Milk acceptance test was carried out. CPSC decreased milk C12:0 (10%, p = 0.023) and C16:0 (5%, p = 0.035) and increased C18:1 cis-12 (37%, p = 0.006), C18:1 trans-11 (32%, p = 0.005), C18:2 cis-9 cis-12 (13%, p = 0.004), and cis-9 trans-11 CLA (35%, p = 0.004). CPSC increased total trans-monounsaturated FA (21%, p = 0.003), total CLA (31%, p = 0.007), and PUFA:SFA ratio (18%, p = 0.006). CPSC did not affect milk production, DMD, DMI and milk composition, but reduced fat yield (9%, p = 0.013) and FCM (7%, p = 0.013). CPSC improved milk overall acceptability. In conclusion, CPSC could modify milk FA profile without a detrimental effect on digestibility, production performance, or milk acceptance.

Production characteristics of the Jamaica Hope breed of dairy cattle

1970 ◽  
Vol 74 (3) ◽  
pp. 463-468 ◽  
Author(s):  
K. E. Wellington ◽  
P. Mahadevan ◽  
K. L. Roache
Keyword(s):  
Dairy Cattle ◽  
Milk Production ◽  
Milk Yield ◽  
Reproductive Performance ◽  
Production Performance ◽  
Tropical Environment ◽  
Calving Interval ◽  
Considerable Potential ◽  
Production Characteristics

SUMMARYThis study is the first comprehensive estimation of breed parameters for the Jamaica Hope breed of dairy cattle. It has shown that the breed has considerable potential for milk production undser good husbendry in a tropical environment. Improvements in reproductive performance may be achieved, without prejudice to production performance, by using milk yield per day of calving interval (which has a moderatoly high repestability) as a criterion for selection.

Performance evaluation, conservation and improvement of Sahiwal cattle in India

2001 ◽  
Vol 31 ◽  
pp. 43-54 ◽  
Author(s):  
B.K. Joshi ◽  
A. Singh ◽  
R.S. Gandhi
Keyword(s):  
Milk Production ◽  
Milk Yield ◽  
Central Government ◽  
Weighted Average ◽  
Zebu Cattle ◽  
Progeny Testing ◽  
Lactation Length ◽  
Frozen Semen ◽  
Sahiwal Cattle ◽  
Brown Body

SummaryThe Sahiwal cattle, one of the best dairy breeds of Zebu cattle in India and Pakistan, originate from the Montgomery district of Pakistan and is distributed on farmer herds in certain pockets of the bordering districts of Punjab and Rajsthan in India. The animals of this breed are also available in Kenya and are used for crossing with local East African Zebu types to improve milk production. Sahiwal cattle have deep body, loose skin, short legs, stumpy horns and a broad head with pale red to dark brown body colour. The average body weight in adult females and males is around 350 and 500 kg, respectively. The animals of this breed are maintained on various State and Central Government farms, privately owned farms, charitable trusts and a small proportion of animals are also available with the farmers. More than 1 200 breedable females are available at various farms in the country. The average lactation milk yield of Sahiwal cattle on organized farms ranges between 1 500 to 2 500 kg. However, in well-managed herds, the highest lactation milk production in certain cows is more than 4 500 kg. The overall weighted average milk yield, age at first calving, lactation length and calving interval based on the performance at various herds is around 1 900 kg, 36 months, 315 days and 420 days, respectively. The fat and Solid Non Fat (SNF) percent ranges from 4.6 to 5.2 percent and 8.9 to 9.3 percent, respectively. Quite a large proportion of pure-bred Sahiwal cattle maintained on organized breeding farms has been used for the production of cross-bred cattle. As a result, different cross-bred strains of dairy cattle viz Karan Swiss, Karan Fries and Frieswal have evolved at the National Dairy Research Institute, Karnal and Military Dairy Farms. The breed has also been utilized for the production of synthetic strains like Jamaica Hope (JH), Australian Milking Zebu (AMZ) and Australian Friesian Sahiwal (AFS) in other countries. Currently, efforts are being made to characterize, evaluate and conserve the breed in field conditions. More than 0.10 million doses of frozen semen of this breed are cryopreserved at various semen banks in the country. The frozen semen is being utilized for strengthening and genetically improving the existing herds of the breed through progeny testing programmes of sires associating various herds of Sahiwal in the country.

scholarly journals Associations between polymorphisms of SLC22A7, NGFR, ARNTL and PPP2R2B genes and Milk production traits in Chinese Holstein

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Ruike Jia ◽  
Yihan Fu ◽  
Lingna Xu ◽  
Houcheng Li ◽  
Yanhua Li ◽  
...  
Keyword(s):  
Dairy Cattle ◽  
Milk Production ◽  
Association Analysis ◽  
Milk Yield ◽  
Production Traits ◽  
Milk Production Traits ◽  
Fat Percentage ◽  
Protein Percentage ◽  
Fat Yield ◽  
Percentage Protein

Abstract Background Our preliminary work confirmed that, SLC22A7 (solute carrier family 22 member 7), NGFR (nerve growth factor receptor), ARNTL (aryl hydrocarbon receptor nuclear translocator like) and PPP2R2B (protein phosphatase 2 regulatory subunit Bβ) genes were differentially expressed in dairy cows during different stages of lactation, and involved in the lipid metabolism through insulin, PI3K-Akt, MAPK, AMPK, mTOR, and PPAR signaling pathways, so we considered these four genes as the candidates affecting milk production traits. In this study, we detected polymorphisms of the four genes and verified their genetic effects on milk yield and composition traits in a Chinese Holstein cow population. Results By resequencing the whole coding region and part of the flanking region of SLC22A7, NGFR, ARNTL and PPP2R2B, we totally found 20 SNPs, of which five were located in SLC22A7, eight in NGFR, three in ARNTL, and four in PPP2R2B. Using Haploview4.2, we found three haplotype blocks including five SNPs in SLC22A7, eight in NGFR and three in ARNTL. Single-SNP association analysis showed that 19 out of 20 SNPs were significantly associated with at least one of milk yield, fat yield, fat percentage, protein yield or protein percentage in the first and second lactations (P < 0.05). Haplotype-based association analysis showed that the three haplotypes were significantly associated with at least one of milk yield, fat yield, fat percentage, protein yield or protein percentage (P < 0.05). Further, we used SOPMA software to predict a SNP, 19:g.37095131C > T in NGFR, changed the structure of NGFR protein. In addition, we used Jaspar software to found that four SNPs, 19:g.37113872C > G,19:g.37113157C > T, and 19:g.37112276C > T in NGFR and 15:g.39320936A > G in ARNTL, could change the transcription factor binding sites and might affect the expression of the corresponding genes. These five SNPs might be the potential functional mutations for milk production traits in dairy cattle. Conclusions In summary, we proved that SLC22A7, NGFR, ARNTL and PPP2R2B have significant genetic effects on milk production traits. The valuable SNPs can be used as candidate genetic markers for genomic selection of dairy cattle, and the effects of these SNPs on other traits need to be further verified.

The effects of abomasal infusions of glucose or casein on milk yield and on some blood constituents of dairy cows in early and late lactations

1984 ◽  
Vol 1984 ◽  
pp. 73-73
Author(s):  
L. Istasse ◽  
E.R. Ørskov
Keyword(s):  
Dairy Cows ◽  
Protein Content ◽  
Milk Production ◽  
Milk Yield ◽  
Blood Parameters ◽  
Protein Yield ◽  
Blood Constituents ◽  
Clear Cut ◽  
Abomasal Infusion ◽  
Fat Yield

Abomasal infusion of casein has been shown to increase the milk yield, fat yield, protein content and protein yield while no clear-cut effects were observed with infusion of glucose (Ørskov, Grubb and Kay, 1977 and confirmed by Oldham, Bines and MacRae, 1983). Little information is available about the changes in blood parameters with abomasal infusion of glucose or casein. The objective of the present experiments was to relate changes in milk production to blood parameters in dairy cows given casein or glucose as an infusion into the abomasum during early or late lactation.

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