Comparative Analysis of Defence Response of Soybean by Seed Soaking in Gibberellic Acid to Salinity

2020 ◽  
Author(s):  
Burcu Seckin Dinler ◽  
Volkan Gul
Keyword(s):  
Reactive Oxygen Species ◽  
Gibberellic Acid ◽  
Enzyme Activities ◽  
Biomass Yield ◽  
Reactive Oxygen ◽  
Antioxidant Enzyme Activities ◽  
Oxygen Species ◽  
Comparative Response ◽  
Glycine Max L ◽  
Soybean Plants

The present study was conducted to determine the effects of gibberellic acid (GA3) presoaking of soybean (Glycine max L.) seeds on the biomass yield, regulation of reactive oxygen species and some antioxidant enzyme activities in radicle, hypocotyl and cotyledons under salinity. Within this scope, the seeds were subjected to priming with 150 ppm GA3 for 6 h at room temperature. Then, 150 mM NaCl was added in a group and the control was not primed with GA3. The results clearly revealed that salt stress caused a slight induction of biomass yield of radicle and hypocotyl, photosynthetic pigments in cotyledon, reactive oxygen species content in all organs. Moreover, GA3 pretreatment alleviated salt-induced oxidative damage by maintaining superoxide dismutase, ascorbate peroxidase and glutathione reductase enzyme activities in soybean plants. This study firstly elucidates the effects of GA3 priming on the comparative response mechanisms to salinity with different organs of soybean plants.

scholarly journals KETERKAITAN EKSTRAK TELUR KEONG MAS DENGAN TINGKAT KETAHANAN SALINITAS BENIH KEDELAI (Glycine max (L.) Merrill)

2019 ◽  
Vol 6 (2) ◽  
pp. 26-37
Author(s):  
Syukri ◽  
Risky Ridha
Keyword(s):  
Reactive Oxygen Species ◽  
Glycine Max ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Glycine Max L

Wilayah pesisir mempunyai potensi cukup besar untuk dikembangkan menjadi lahan pertanian, namun peningkatan muka air laut akan menyebabkan terjadinya salinitas. Salah satu penyebab kerusakan tanaman pada kondisi salinitas adalah terjadinya cekaman oksidatif yang disebabkan oleh terakumulasinya senyawa Reactive Oxygen Species (ROS). Radikal ini dapat menyebabkan degradasi membran sel yang dapat menyebabkan hilangnya energi untuk biosintesis, cadangan makanan di embrio menjadi habis dan menurunnya viabilitas dan vigoritas benih. Penelitian ini bertujuan untuk mengkaji seberapa besar pengaruh dari ekstrak telur keong mas sebagai antioksidan alami dalam meningkatkan vigoritas benih kedelai (Glycine max (L.) Merril) pada kondisi cekaman salinitas. Penelitian disusun menggunakan Rancangan Acak Lengkap (RAL) faktorial dengan tiga ulangan. Sebagai faktor pertama yaitu kosentrasi ekstrak telur keong mas yang terdiri dari : E1 (45 %) dan E2 (0 %). faktor kedua yaitu kosentrasi larutan NaCl yang terdiri dari : N0 (NaCl 0,0 %), N1 (NaCl 0,5 %), N2 (NaCl 1,0 %) dan N3 (NaCl 1,5 %). Hasil penelitian menunjukkan bahwa pemberian ekstrak telur keong mas kosentrasi 45 % hanya dapat meningkatkan daya berkecambah sebesar 51,59 %, kecepatan tumbuh sebesar 24,18 %/etmal dan vigor kecambah sebesar 33,55 % pada kondisi cekaman salinitas hingga 0,5 %, namun pada cekaman salinitas 1,0 % dan 1,5 % benih sudah tidak mampu untuk berkecambah normal. Peningkatan cekaman salinitas     0 % - 1,0 % menyebabkan pengurangan panjang hipokotil dan panjang akar primer benih kedelai. Pemberian ekstrak telur keong mas kosentrasi 45 % menghasilkan hipokotil dan akar primer benih kedelai yang lebih panjang dibandingkan dengan tanpa pemberian ekstrak. Hasil penelitian membuktikan adanya perbaikan viabilitas dan vigoritas benih yang ditunjukkan oleh indikasi fisiologi yaitu perbaikan performansi panjang hipokotil dan akar primer, meningkatkan nilai kecepatan tumbuh dan vigor kecambah benih kedelai dengan perendaman ekstrak telur keong mas pada kondisi salinitas walaupun pada level yang rendah.

scholarly journals Soybean Plant Metabolism under Water Deficit and Xenobiotic and Antioxidant Agent Application

Biology ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 266
Author(s):  
Julia Renata Schneider ◽  
Mariele Müller ◽  
Vilson Antonio Klein ◽  
Luciana Grazziotin Rossato-Grando ◽  
Rômulo Pillon Barcelos ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Gas Exchange ◽  
Soil Water ◽  
Water Deficit ◽  
Water Availability ◽  
Water Status ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Antioxidant Agent ◽  
Soybean Plants

The aim was to evaluate the interactive effects on biochemistry and physiology of soybean plants exposed to simultaneous xenobiotic and water deficit stresses, and the possible attenuation of plant damage by an antioxidant agent. Soybean plants were submitted to eight different soil water potentials, in two experiments (first experiment: −0.96, −0.38, −0.07, −0.02 MPa, and second experiment: −3.09, −1.38, −0.69, −0.14 MPa), xenobiotic, and antioxidant agent applications. Was observed a reduction in water status, gas exchange, photosynthetic pigments, photosystem II quantum yield, and increased leaf temperature in plants under low water availability. Water deficit also induced oxidative stress by the increased production of reactive oxygen species, cellular and molecular damage, and induction of the antioxidant defense metabolism, reduction of gas exchange, water status, and photosynthetic efficiency. The xenobiotic application also caused changes, with deleterious effects more pronounced in low soil water availability, mainly the reactive oxygen species production, consequently the antioxidant activity, and the oxidative damages. This indicates different responses to the combination of stresses. Antioxidant enzyme activity was reduced by the application of the antioxidant agent. Principal Component Analysis showed a relation with the antioxidant agent and reactive oxygen species, which is probably due to signaling function, and with defense antioxidant system, mainly glutathione, represented by thiols.

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