Impact of Seed Maturation and Drought on Seed Germination and Early Seedling Growth in White Clover (Trifolium repens L.)

Author(s):  
Ruqiang Tong ◽  
Xinyuan Liu ◽  
Bifan Mu ◽  
Junfeng Wang ◽  
Mengxing Liu ◽  
...  

Background: Understanding the drought tolerance of white clover seeds during germination and early seedling growth and how that is linked to seed maturation in legume species, is crucial to developing new stand establishments.Methods: An experiment was conducted in Randomized Block design in two factorial concept to explore how seed maturation and drought tolerance influence seed germination and early seedling growth using the two factors: seed coat color (yellow and brown, two levels as one factor) and drought intensity (control: 0 MPa [distilled water], mild drought: -0.2 MPa, moderate drought: -0.4 MPa and severe drought: -0.6 MPa, four levels as another factor).Result: In contrast to the yellow-coated seeds, the mean germination percentage, germination index, seedling vigour index and radicle length of the brown-coated seeds were lowered by 7.9%, 23.1%, 20.5% and 12.9%, respectively. Compared to the control, introducing drought conditions reduced the mean of germination percentage, germination index, seedling vigour index and radicle length of yellow-coated seeds by 52.4%, 64.1%, 41.9% and 23.2%, respectively. Meanwhile, drought affected brown-coated seeds more than yellow-coated seeds. The results indicating that the seed quality of white clover seed lots with yellow-colored coats higher than the seed lots with brown-coloured coats. In agronomic practice, a larger proportion of yellow-coated seeds should be harvested to improve new stands successful establishment under environment easy to dry.

Author(s):  
R. Himaja ◽  
K. Radhika ◽  
K. Bayyapu Reddy ◽  
M. Raghavendra

Background: Drought stress at germination and early growth stages hinders the seedling establishment in chickpea which ultimately affects the economic yield. Such adverse affects of drought can be mitigated by screening and identifying the tolerant genotypes of chickpea which is commonly cultivated under rain-fed conditions during post-rainy season. Methods: Effect of drought stress on germination and early seedling growth of thirty three chickpea genotypes was studied under four different concentrations of PEG 6000 (-0.3, -0.6, -0.9 and -1.2 MPa) along with control and hydration under laboratory conditions during 2018-19. Result: Significant variation was observed among the genotypes for germination, root length, shoot length and seedling vigour index under different concentrations of PEG 6000. Complete inhibition of germination was observed in most of the genotypes at -1.2 MPa. Based on the results obtained, JG 11 and NBeG 3 were considered as tolerant since they showed comparatively higher germination, root length, shoot length and seedling vigour even at -1.2 MPa, while NBeG 723 and NBeG 833 were considered as susceptible genotypes because of their poor germination and seedling growth even at lower levels of drought stress.


2015 ◽  
Vol 42 (2) ◽  
pp. 209 ◽  
Author(s):  
Linda Gorim ◽  
Folkard Asch

Seed germination and the successful establishment of young seedlings is an important aspect of plant life. Seed coats are used to improve stand establishment and early seedling vigour. Seedlings growing from hydro-absorber coated barley, rye and wheat with coat-shares greater than 75% of the average seed have been shown to promote better seedling growth compared with those seedlings growing from uncoated seeds. We investigated how and why these seedlings performed better by analysing the proportion of grain reserves mobilised for growth and respiration as well as how both sucrose and glucose available in the embryo translated into seedling growth in the presence or absence of seed coats containing hydro-absorber gel. We found that mobilisation efficiency was higher, resulting in higher biomass in these cereals when they were coated. The relationship between sucrose and glucose available to the seedling as well as its correlation with early seedling growth indicate a switch in the enzymatic cleavage of embryonic sucrose from invertase to sucrose synthase. This in turn indicates that in coated seeds, embryonic tissue must be hypoxic leading to a more efficient use of glucose and thus reduced respiration losses during germination.


2018 ◽  
Vol 7 (1) ◽  
pp. 35-44
Author(s):  
FM Tonmoy Chowdhury ◽  
MA Halim ◽  
Feroza Hossain ◽  
Nahid Akhtar

The response of BARI Sunflower-2 (Helianthus annuus L.) to sodium chloride (NaCl) at germination and early seedling growth was investigated. The seeds and seedlings of sunflower were treated with a series of eight different concentrations of NaCl viz 25, 50, 75, 100, 125, 150, 175 and 200 mM and distilled water (control) and were allowed to grow under plate culture condition. The results showed that the highest salinity concentration (200 mM NaCl) remarkably decreased the germination percentage, germination index and speed of germination of BARI Sunflower-2. Fresh and dry masses of both shoot and root were significantly reduced at a 25-200 mM NaCl solution. Significant reductions of shoot and root length were also noted with the increase of NaCl concentration. The results also illustrate that up to 50 mM NaCl concentration, all of the germination indices and fresh and dry biomass were withstand more than 70%. Thus the present study concluded that the BARI Sunflower-2 could be cultivated in moderately saline soil.Jahangirnagar University J. Biol. Sci. 7(1): 35-44, 2018 (June)


2021 ◽  
Vol 10 (2) ◽  
pp. 191-195
Author(s):  
Hana A. Al-Quhbi ◽  
Amna A.I. Saeed ◽  
Abdul Nasser Al-Gifri

Allelopathic effect of Aqueous decomposed leaf litter from four common trees and shrubs in Aden governorate, Yemen was investigated on the germination and early seedling growth of Cowpea, the test crop was subjected to four concentrations of 25, 50, 75 and 100% of the aqueous extract, while a control was maintained at 0% extract level. The study carried out in the laboratory of Botany at Faculty of Education, University of Aden. The Percentage of germination, Germination rate per plate, radicle length, plumule, (hypo and epicotyl lengths) observed. Data was recorded at 24 hours interval after germination for 15 days, the research revealed delayed germination rate per plate and a significant reduction in the radicle length and germination percentage (data was collected on percentage germination; plant height. Results obtained showed that significant reduction in the growth parameters considered at 100% and 75%, while at 50% and 25% the difference was not significant. From the result obtained, it can concluded that Conocarpus lancefolius and Thevetia peruviana possess allelopathic effect that inhibit the germination and early seedling growth of Cowpea, hence at 100% concentration.


2014 ◽  
Vol 66 (1) ◽  
pp. 99-108 ◽  
Author(s):  
Morteza Saberi ◽  
Farajollah Tamian

Abstract The objective of this study was to evaluate the effectiveness of seed priming in improving seed germination and seedling vigor of Vicia villosa under laboratory conditions. Chemical stimulators included: gibberel-lic acid (125,250 and 500 ppm), salicylic acid (100,200 and 300 mg/lit) and extract of Eucalyptus camaldulen-sis (0, 25, 50, 75 and 100 %). This experiment was carried out as factorial experiment based on a randomized completely design, with four replications. The results showed that Eucalyptus camaldulensis extract had in-hibitive effect on germination and early seedling growth of Vicia villosa. Early seedling growth of Vicia villosa increased by pretreatment of seeds in chemical stimulators so that the highest effect was observed in gibberellic acid (250 ppm). The chemical stimulators don’t have any effect on germination speed. Interaction effects of allelopathic and pretreatment with chemical stimulators were significance on germination percentage, root, shoot and plant length and seed vigor index.


2017 ◽  
Vol 28 (1) ◽  
pp. 18-25 ◽  
Author(s):  
R Ahmed ◽  
MHK Howlader ◽  
A Shila ◽  
MA Haque

An incubation experiment was conducted at the Department of Soil Science, Patuakhali Science and Technology University during Rabi season 2015 to investigate the effects of different levels of salinity on germination and early seedling growth of maize. There were ten treatments consisting of different concentrations of salinity viz. 0, 20, 40, 80, 120, 160, 200, 240, 280 and 320 mM NaCl. The germination experiment was conducted in petri dish lined with a layer of cotton consisting ten ml of each test solution. Germination percentage gradually decreased with the increase of concentration of salt. Up to 80 mM concentration was found safe for maize seed germination. Salinity caused delay in germination.  Highest seedling height was found at 40 mM NaCl concentration (21.51 cm) and root length (23.61 cm) was found in 20 mM NaCl concentration. In 320 mM NaCl concentration roots were abnormal, deformed and twisted. The 0 to 80 mM NaCl concentration gave statistically similar shoot fresh weight. Compared to control treatment 20 mM NaCl concentration gave 0.55 % higher fresh weight and at 80 mM NaCl concentration shoot fresh weight reduced by only 6.9 %. The 80 mM NaCl concentration was found acceptable for germination and early seedling growth of maize.Progressive Agriculture 28 (1): 18-25, 2017


2014 ◽  
Vol 9 (4) ◽  
pp. 374-382 ◽  
Author(s):  
Ilona Czyczyło-Mysza ◽  
Izabela Marcińska ◽  
Edyta Skrzypek ◽  
Katarzyna Cyganek ◽  
Katarzyna Juzoń ◽  
...  

AbstractThe QTLs controlling germination and early seedling growth were mapped using seeds acquired from mapping population and parental lines of Chinese Spring and SQ1 grown under water-limited conditions, severe drought (SDr) and well-watered plants (C). Germination ability was determined by performing a standard germination test based on the quantification of the germination percentage (GP24) of seeds incubated for 24 h at 25°C in the dark. Early seedling growth was evaluated on the basis of the length of the root and leaf at the 6th day of the experiment. QTLs were identified by composite interval mapping method using Windows QTLCartographer 2.5 software. For the traits studied, a total of thirty eight additive QTLs were identified. Seventeen QTLs were mapped in C on chromosomes: 1A, 2A, 7A, 1B, 2B, 3B, 4B, 5B, 6B, 7B, 2D, 3D, 4D and 6D, while twenty one QTLs were identified in SDr on chromosomes: 1A, 2A, 5A, 2B, 3B, 4B, 5B, 6B, 7B, 3D, 5D and 6D. Most of the QTLs for GP and early leaf growth parameters were clustered on chromosome 4B (associated with the Rht-B1 marker) both in C and SDr plants. The results indicate the complex and polygenic nature of germination.


2016 ◽  
Vol 57 (8) ◽  
pp. 1689-1706 ◽  
Author(s):  
Tory Chhun ◽  
Suet Yen Chong ◽  
Bong Soo Park ◽  
Eriko Chi Cheng Wong ◽  
Jun-Lin Yin ◽  
...  

Plants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1166
Author(s):  
Mohamed Kouighat ◽  
Hafida Hanine ◽  
Mohamed El Fechtali ◽  
Abdelghani Nabloussi

In the context of climate change and water scarcity, there is a need to develop and use drought-tolerant sesame cultivars. This study was conducted to evaluate the response of 13 sesame genotypes, including 11 mutants and their wild-types, to drought during germination and early seedling growth. Moderate and severe drought stress was simulated by applying polyethylene glycol (PEG) at two osmotic potentials, −0.6 MPa and −1.2 MPa, respectively, on seeds of two successive mutant generations, M2 and M3. The parameters measured or calculated were germination percentage (GP), germination rate (GR), mean germination time (MGT), root length (RL), shoot length (SL), root to shoot ratio (RSR), and the seedling vigor index (SVI). Results showed the significant effect of genotype, drought, and drought × genotype interaction on all parameters investigated. Under severe drought, seeds of seven genotypes, including wild types, were not able to germinate. There was a drastic decline of all parameters for the rest, except MGT and RSR, which markedly increased. Interestingly, two mutants, “ML2-5” and “ML2-10”, were identified as the most tolerant to severe drought and the most stable over both generations. The present work is the first report of sesame germplasm with such a high level of tolerance to drought during germination and early seedling growth stages.


Author(s):  
Anju B Raj ◽  
Sheeja K Raj ◽  
K. Prathapan ◽  
N.V. Radhakrishnan

The experiment was conducted at Coconut Research Station, Balaramapuram with an objective to study the effect of nutripriming with ZnSO4 and borax on the early growth and seedling vigour and Zn and B content in grain cowpea. Nutripriming treatments had significant effect on germination parameters. Nutripriming with ZnSO4 either at 0.025 or 0.05 per cent recorded higher values for the germination parameters. These treatments recorded 19.68 and 18.51 per centage higher germination than control. Germination index was 1.4 times higher in seeds primed with ZnSO4 @ 0.025 and 0.05 per cent and also recorded higher values for mean daily germination and speed of germination. The highest co-efficient rate of germination and germination rate index was recorded by seed priming with ZnSO4 at 0.05 per cent and it was closely followed by ZnSO4 @ 0.025 per cent for 4 h. Nutripriming with ZnSO4 either @ 0.025 or 0.05 per cent recorded the lowest mean germination time and time to reach 50 per cent germination and also recorded higher Zn and B content. Vigour index also followed the same trend. Hence it can be concluded that nutripriming with ZnSO4 either at 0.025 or 0.05 per cent can be recommended for early seedling growth, seedling vigour and higher Zn and B content in grain cowpea.


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