QTL mapping for germination of seeds obtained from previous wheat generation under drought

2014 ◽  
Vol 9 (4) ◽  
pp. 374-382 ◽  
Author(s):  
Ilona Czyczyło-Mysza ◽  
Izabela Marcińska ◽  
Edyta Skrzypek ◽  
Katarzyna Cyganek ◽  
Katarzyna Juzoń ◽  
...  
Keyword(s):  
Seedling Growth ◽  
Growth Parameters ◽  
Leaf Growth ◽  
Interval Mapping ◽  
Germination Percentage ◽  
Mapping Method ◽  
Severe Drought ◽  
Early Seedling Growth ◽  
Germination Ability ◽  
Early Seedling

AbstractThe QTLs controlling germination and early seedling growth were mapped using seeds acquired from mapping population and parental lines of Chinese Spring and SQ1 grown under water-limited conditions, severe drought (SDr) and well-watered plants (C). Germination ability was determined by performing a standard germination test based on the quantification of the germination percentage (GP24) of seeds incubated for 24 h at 25°C in the dark. Early seedling growth was evaluated on the basis of the length of the root and leaf at the 6th day of the experiment. QTLs were identified by composite interval mapping method using Windows QTLCartographer 2.5 software. For the traits studied, a total of thirty eight additive QTLs were identified. Seventeen QTLs were mapped in C on chromosomes: 1A, 2A, 7A, 1B, 2B, 3B, 4B, 5B, 6B, 7B, 2D, 3D, 4D and 6D, while twenty one QTLs were identified in SDr on chromosomes: 1A, 2A, 5A, 2B, 3B, 4B, 5B, 6B, 7B, 3D, 5D and 6D. Most of the QTLs for GP and early leaf growth parameters were clustered on chromosome 4B (associated with the Rht-B1 marker) both in C and SDr plants. The results indicate the complex and polygenic nature of germination.

scholarly journals Allelopathic activity of aqueous decomposed leaf litter extracts on the germination and early seedling growth of cowpea (Vigna unguiculata) in Aden, Yemen

2021 ◽  
Vol 10 (2) ◽  
pp. 191-195
Author(s):  
Hana A. Al-Quhbi ◽  
Amna A.I. Saeed ◽  
Abdul Nasser Al-Gifri
Keyword(s):  
Leaf Litter ◽  
Seedling Growth ◽  
Growth Parameters ◽  
Germination Rate ◽  
Germination Percentage ◽  
Allelopathic Effect ◽  
Early Seedling Growth ◽  
Early Seedling ◽  
The Difference ◽  
Radicle Length

Allelopathic effect of Aqueous decomposed leaf litter from four common trees and shrubs in Aden governorate, Yemen was investigated on the germination and early seedling growth of Cowpea, the test crop was subjected to four concentrations of 25, 50, 75 and 100% of the aqueous extract, while a control was maintained at 0% extract level. The study carried out in the laboratory of Botany at Faculty of Education, University of Aden. The Percentage of germination, Germination rate per plate, radicle length, plumule, (hypo and epicotyl lengths) observed. Data was recorded at 24 hours interval after germination for 15 days, the research revealed delayed germination rate per plate and a significant reduction in the radicle length and germination percentage (data was collected on percentage germination; plant height. Results obtained showed that significant reduction in the growth parameters considered at 100% and 75%, while at 50% and 25% the difference was not significant. From the result obtained, it can concluded that Conocarpus lancefolius and Thevetia peruviana possess allelopathic effect that inhibit the germination and early seedling growth of Cowpea, hence at 100% concentration.

scholarly journals First Report of Sesame Mutants Tolerant to Severe Drought Stress during Germination and Early Seedling Growth Stages

Plants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1166
Author(s):  
Mohamed Kouighat ◽  
Hafida Hanine ◽  
Mohamed El Fechtali ◽  
Abdelghani Nabloussi
Keyword(s):  
Drought Stress ◽  
Seedling Growth ◽  
Germination Rate ◽  
Germination Percentage ◽  
Growth Stages ◽  
Severe Drought ◽  
First Report ◽  
Early Seedling Growth ◽  
Early Seedling ◽  
Sesame Genotypes

In the context of climate change and water scarcity, there is a need to develop and use drought-tolerant sesame cultivars. This study was conducted to evaluate the response of 13 sesame genotypes, including 11 mutants and their wild-types, to drought during germination and early seedling growth. Moderate and severe drought stress was simulated by applying polyethylene glycol (PEG) at two osmotic potentials, −0.6 MPa and −1.2 MPa, respectively, on seeds of two successive mutant generations, M2 and M3. The parameters measured or calculated were germination percentage (GP), germination rate (GR), mean germination time (MGT), root length (RL), shoot length (SL), root to shoot ratio (RSR), and the seedling vigor index (SVI). Results showed the significant effect of genotype, drought, and drought × genotype interaction on all parameters investigated. Under severe drought, seeds of seven genotypes, including wild types, were not able to germinate. There was a drastic decline of all parameters for the rest, except MGT and RSR, which markedly increased. Interestingly, two mutants, “ML2-5” and “ML2-10”, were identified as the most tolerant to severe drought and the most stable over both generations. The present work is the first report of sesame germplasm with such a high level of tolerance to drought during germination and early seedling growth stages.

scholarly journals Assessment on the Effects of Potting Media on Seed Germination and Early Seedling Growth of Pterocarpus erinaceus Poir

2021 ◽  
Vol 25 (6) ◽  
pp. 969-975
Author(s):  
M.K. Peter ◽  
SIN Agera ◽  
J.I. Amonum
Keyword(s):  
Seed Germination ◽  
Seedling Growth ◽  
Growth Parameters ◽  
Cow Dung ◽  
Early Seedling Growth ◽  
Top Soil ◽  
Early Seedling ◽  
Potting Media ◽  
Number Of Leaves ◽  
Poultry Droppings

This study investigated the effects of potting media on seed germination and early seedling growth of Pterocarpus erinaceus Poir at the Forestry Nursery in Jos, Nigeria. Using Completely Randomized Design (CRD) with three replicates, laboratory-tested soil samples, top soil, sharp sand, sharp sand + top soil, sharp sand + top soil + cow dung and sharp sand + top soil + poultry droppings were used in various combinations to assess the growth parameters of P. erinaceus (germination percentage, emergence, plant height, number of leaves, length of leaves and stem diameter) for 12 weeks. Descriptive and inferential statistics were employed to analyze collected data. Result indicated that sharp sand + top soil + poultry droppings had the highest nitrogen concentration (2.19%), sharp sand + top soil + cow dung (2.07%), sharp sand + top soil (1.50%), top soil (0.72%) and Sharp sand (0.38%). Potting media with poultry droppings recorded an overall higher percentage germination of 42.9% by the end of the germination period. Analysis of variance (ANOVA) of collected data on combined soil aggregate on growth parameters indicated a significant (p<0.05) difference in plant height, number of leaves, leaf length and stem diameter. Potting with poultry dropping gave the best potting media growth results when compared to other treatments that enhanced seed germination and seedling growth of P. erinaceus. This superior observation of the poultry droppings incorporated potting mixtures over the cow dung provides an outstanding potentials to enhance P. erinaceus plantation establishment. Consequently, recommended for raising seedlings in the nursery as well as ensuring sustainable management.

scholarly journals The Use of Germination Indices for Salinity Tolerance Classification of Pepper Cultivars

2019 ◽  
Vol 7 (9) ◽  
pp. 1304
Author(s):  
Gamze Kaya
Keyword(s):  
Salinity Tolerance ◽  
Seedling Growth ◽  
Growth Parameters ◽  
Genotypic Variation ◽  
Germination Percentage ◽  
Control Treatment ◽  
Tolerance Index ◽  
Seedling Length ◽  
Early Seedling ◽  
Germination Indices

The study aimed to evaluate the use of germination indices as a screening tool for salinity tolerance during germination and early seedling growth of pepper cultivars, and to distinguish the potential for genetic responses to salt tolerance. In the study, the seeds of seven pepper cultivars were germinated at increasing NaCl levels of 5, 10, 15 and 20 dS/m and distilled water as the control treatment for 14 days. Germination percentage (GP), mean germination time (MGT), germination index (GI), germination stress tolerance index (GSTI), seedling length (SL), seedling fresh weight (SFW) and vigor index (VI) were investigated. Results showed that germination percentage decreased with increasing NaCl levels while the highest germination percentage at 20 dS/m was 92% in BT Burdem with no significant reduction. Seedling growth of pepper cultivars was severely inhibited by increasing salinity stress. SFW was depressed depending on reduction in SL due to increasing NaCl. BT-Burli and BT İnce Sivri were the most tolerant cultivars to NaCl and they were used for genetic resources towards salinity. Seedling growth was much more sensitive to salinity than germination because of the highest percent reduction in seedling growth parameters. Among the parameters, GSTI gave the highest significant correlation coefficient with SL and SFW; indicating that it would be useful for estimating seedling growth. It was concluded that genotypic variation was observed among pepper cultivars for salinity tolerance and GSTI could be used for a predictor for salinity tolerance.

scholarly journals Effects of sodium chloride on germination and seedling growth of Sunflower (Helianthus annuus L.)

2018 ◽  
Vol 7 (1) ◽  
pp. 35-44
Author(s):  
FM Tonmoy Chowdhury ◽  
MA Halim ◽  
Feroza Hossain ◽  
Nahid Akhtar
Keyword(s):  
Sodium Chloride ◽  
Helianthus Annuus ◽  
Seedling Growth ◽  
Germination Percentage ◽  
Water Control ◽  
Helianthus Annuus L ◽  
Early Seedling Growth ◽  
Nacl Concentration ◽  
Early Seedling ◽  
Germination Indices

The response of BARI Sunflower-2 (Helianthus annuus L.) to sodium chloride (NaCl) at germination and early seedling growth was investigated. The seeds and seedlings of sunflower were treated with a series of eight different concentrations of NaCl viz 25, 50, 75, 100, 125, 150, 175 and 200 mM and distilled water (control) and were allowed to grow under plate culture condition. The results showed that the highest salinity concentration (200 mM NaCl) remarkably decreased the germination percentage, germination index and speed of germination of BARI Sunflower-2. Fresh and dry masses of both shoot and root were significantly reduced at a 25-200 mM NaCl solution. Significant reductions of shoot and root length were also noted with the increase of NaCl concentration. The results also illustrate that up to 50 mM NaCl concentration, all of the germination indices and fresh and dry biomass were withstand more than 70%. Thus the present study concluded that the BARI Sunflower-2 could be cultivated in moderately saline soil.Jahangirnagar University J. Biol. Sci. 7(1): 35-44, 2018 (June)

Impact of Seed Maturation and Drought on Seed Germination and Early Seedling Growth in White Clover (Trifolium repens L.)

2021 ◽  
Author(s):  
Ruqiang Tong ◽  
Xinyuan Liu ◽  
Bifan Mu ◽  
Junfeng Wang ◽  
Mengxing Liu ◽  
...  
Keyword(s):  
Seedling Growth ◽  
White Clover ◽  
Germination Percentage ◽  
Seed Maturation ◽  
Percentage Germination ◽  
Seedling Vigour ◽  
Early Seedling Growth ◽  
Vigour Index ◽  
Early Seedling ◽  
The Mean

Background: Understanding the drought tolerance of white clover seeds during germination and early seedling growth and how that is linked to seed maturation in legume species, is crucial to developing new stand establishments.Methods: An experiment was conducted in Randomized Block design in two factorial concept to explore how seed maturation and drought tolerance influence seed germination and early seedling growth using the two factors: seed coat color (yellow and brown, two levels as one factor) and drought intensity (control: 0 MPa [distilled water], mild drought: -0.2 MPa, moderate drought: -0.4 MPa and severe drought: -0.6 MPa, four levels as another factor).Result: In contrast to the yellow-coated seeds, the mean germination percentage, germination index, seedling vigour index and radicle length of the brown-coated seeds were lowered by 7.9%, 23.1%, 20.5% and 12.9%, respectively. Compared to the control, introducing drought conditions reduced the mean of germination percentage, germination index, seedling vigour index and radicle length of yellow-coated seeds by 52.4%, 64.1%, 41.9% and 23.2%, respectively. Meanwhile, drought affected brown-coated seeds more than yellow-coated seeds. The results indicating that the seed quality of white clover seed lots with yellow-colored coats higher than the seed lots with brown-coloured coats. In agronomic practice, a larger proportion of yellow-coated seeds should be harvested to improve new stands successful establishment under environment easy to dry.

scholarly journals Effect of Seed Priming on Improvement of Germination of Vicia Villosa Under Allelopathic Components of Eucalyptus Camaldulensis

2014 ◽  
Vol 66 (1) ◽  
pp. 99-108 ◽  
Author(s):  
Morteza Saberi ◽  
Farajollah Tamian
Keyword(s):  
Seedling Growth ◽  
Eucalyptus Camaldulensis ◽  
Seed Priming ◽  
Germination Percentage ◽  
Seed Vigor ◽  
Vicia Villosa ◽  
Early Seedling Growth ◽  
Vigor Index ◽  
Early Seedling ◽  
Plant Length

Abstract The objective of this study was to evaluate the effectiveness of seed priming in improving seed germination and seedling vigor of Vicia villosa under laboratory conditions. Chemical stimulators included: gibberel-lic acid (125,250 and 500 ppm), salicylic acid (100,200 and 300 mg/lit) and extract of Eucalyptus camaldulen-sis (0, 25, 50, 75 and 100 %). This experiment was carried out as factorial experiment based on a randomized completely design, with four replications. The results showed that Eucalyptus camaldulensis extract had in-hibitive effect on germination and early seedling growth of Vicia villosa. Early seedling growth of Vicia villosa increased by pretreatment of seeds in chemical stimulators so that the highest effect was observed in gibberellic acid (250 ppm). The chemical stimulators don’t have any effect on germination speed. Interaction effects of allelopathic and pretreatment with chemical stimulators were significance on germination percentage, root, shoot and plant length and seed vigor index.

scholarly journals Effect of salinity on germination and early seedling growth of maize

2017 ◽  
Vol 28 (1) ◽  
pp. 18-25 ◽  
Author(s):  
R Ahmed ◽  
MHK Howlader ◽  
A Shila ◽  
MA Haque
Keyword(s):  
Seedling Growth ◽  
Petri Dish ◽  
Germination Percentage ◽  
Control Treatment ◽  
Fresh Weight ◽  
Shoot Fresh Weight ◽  
Early Seedling Growth ◽  
Nacl Concentration ◽  
Early Seedling ◽  
Germination Experiment

An incubation experiment was conducted at the Department of Soil Science, Patuakhali Science and Technology University during Rabi season 2015 to investigate the effects of different levels of salinity on germination and early seedling growth of maize. There were ten treatments consisting of different concentrations of salinity viz. 0, 20, 40, 80, 120, 160, 200, 240, 280 and 320 mM NaCl. The germination experiment was conducted in petri dish lined with a layer of cotton consisting ten ml of each test solution. Germination percentage gradually decreased with the increase of concentration of salt. Up to 80 mM concentration was found safe for maize seed germination. Salinity caused delay in germination.  Highest seedling height was found at 40 mM NaCl concentration (21.51 cm) and root length (23.61 cm) was found in 20 mM NaCl concentration. In 320 mM NaCl concentration roots were abnormal, deformed and twisted. The 0 to 80 mM NaCl concentration gave statistically similar shoot fresh weight. Compared to control treatment 20 mM NaCl concentration gave 0.55 % higher fresh weight and at 80 mM NaCl concentration shoot fresh weight reduced by only 6.9 %. The 80 mM NaCl concentration was found acceptable for germination and early seedling growth of maize.Progressive Agriculture 28 (1): 18-25, 2017

scholarly journals Ameliorative effects of TiO2 nanoparticles and sodium nitroprusside on seed germination and seedling growth of wheat under PEG-stimulated drought stress

2019 ◽  
Vol 41 (3) ◽  
pp. 309-317 ◽  
Author(s):  
Javad Faraji ◽  
Ali Sepehri
Keyword(s):  
Drought Stress ◽  
Seed Germination ◽  
Sodium Nitroprusside ◽  
Tio2 Nanoparticles ◽  
Seedling Growth ◽  
Severe Drought ◽  
Fresh Weight ◽  
Tio2 Nps ◽  
Early Seedling Growth ◽  
Early Seedling

Abstract: Seed germination and early seedling growth are sensitive to drought stress in wheat. A factorial experiment was arranged based on a completely randomized design with three replicates to study the impacts of TiO2 nanoparticles (TiO2 NPs: 0, 500, 1000 and 2000 mg.L) and sodium nitroprusside (SNP: 0 and 100 μM), as NO donor, on seed germination and seedling growth of wheat under polyethylene glycol (PEG)-induced drought stress (0, -0.4 and -0.8 MPa). Our results revealed that PEG-stimulated drought stress significantly decreased germination percentage (GP), germination energy (GE), germination rate (GR), root length (RL), shoot length (SL), root fresh weight (RFW), shoot fresh weight (SFW) and vigor index (VI) but increased mean germination time (MGT) in wheat seeds. However, application of TiO2 NPs and SNP alone or in combination significantly enhanced GP, GE, GR, RL, SL, RFW, SFW and VI up to 23.72%, 50%, 33.74%, 85.38%, 93.28%, 73%, 91.91% and 91.04% respectively, but significantly reduced MGT up to 28.36% under severe drought stress. Our results showed that application of TiO2 NPs and SNP alone or in combination can significantly alleviate the adverse effects of PEG-stimulated drought stress on seed germination and early seedling growth of wheat.

scholarly journals Farklı Tuz Konsantrasyonlarının Şeker Mısır (Zea mays L.Var. sacharata sturt.) Çeşitlerinde Çimlenme ve Erken Fide Gelişimi Üzerine Etkileri

2019 ◽  
Vol 7 (sp2) ◽  
pp. 103
Author(s):  
Hasan Akay ◽  
Elif Öztürk ◽  
İsmail Sezer ◽  
Murat Can Bahadır
Keyword(s):  
Zea Mays ◽  
Seedling Growth ◽  
Dry Matter ◽  
Power Index ◽  
Growth Parameters ◽  
Germination Rate ◽  
Salinity Level ◽  
Stem Length ◽  
Early Seedling Growth ◽  
Early Seedling

This study was carried out to determine the effect of different NaCl concentrations on germination and early seedling growth of some sugar maize (Zea mays L. sacharata sturt.) Varieties. NaCl was applied to the seeds of Vega F1 (sh2), Merit F1 (su) and Tanem F1 (su) varieties at 0 (control), 2, 4, 6, 8 and 10 dSm -1 concentrations. 5 days after seed sowing germination rate, 12 days after the root length, stem length, stem stem ratio, root dry matter ratio, stem dry matter ratio, seedling power index and salt tolerance were measured. Significant differences were found between the characters examined in the study. As a result; It was determined that germination rate and seedling growth characteristics decreased as cultivars salt ratio increased to different salt concentrations. It has been determined that while the total F1 cultivars have the highest values in terms of germination rate and salt stress tolerance, it has the lowest values in terms of root and stem length and root stem ratio. In terms of seedling power index, Vega F1, Tanem F1 and Merit F1, respectively. According to the correlation results, statistically significant positive differences were found between the parameters examined. It was determined that seed germination and early seedling growth parameters were not affected from the germination environment up to 2 dSm-1 salinity level, while it was found to be negatively affected at 2 dSm-1 high levels. It has been determined that water up to 2 dSm-1 salinity level can be used in sugar corn germination and early seedling development periods in aquaculture areas.

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