Genetic Relationship in Sainfoin (Onobrychis viciifolia) Landraces Cultivated East Anatolia by using RAPD and ISSR Markers

2021 ◽  
Author(s):  
Mehmet Macit Ertuş
Keyword(s):  
Genetic Diversity ◽  
Diversity Index ◽  
Calcareous Soils ◽  
Similarity Index ◽  
Issr Markers ◽  
High Genetic Diversity ◽  
Onobrychis Viciifolia ◽  
Young Leaves ◽  
Rapd And Issr ◽  
Issr Primers

Background: Sainfoin (Onobrychis viciifolia) is a forage crop that yields high in arid and calcareous soils and is cultivated in large areas. There aren’t many genetic diversity studies on the varieties of cultured sainfoin. This study was conducted to determine the genetic diversity and the degree of relationship between 23 cultivated landraces and one registered variety. Methods: To take samples from the populations, seeds were sown in the field in 2014. Samples were taken from the young leaves of the plants and preserved at -80oC in same year. RAPD and ISSR primers were used in the study. The bands obtained as a result of PCR were recorded and the data of both methods were also evaluated by combining them. Result: In the study, 5 RAPD and 4 ISSR primers were used and a total of 49 bands were obtained. Of 29 bands obtained using RAPD primers, 20 were found to be polymorphic and of 20 bands obtained using ISSR primers, 15 were found to be polymorphic. It was found that there was a very low correlation between the two methods. Using RAPD and ISSR markers and RAPD + ISSR combination, the similarity index among populations was found to be between 0.25-0.95, 0.5-1.00 and 0.45-0.91, respectively. The Nei’s genetic diversity index was found to be between 0.3365, 0.2656 and 0.3018 with RAPD, ISSR primers and RAPD + ISSR combination, respectively. Based on the dendrograms obtained using RAPD, ISSR primers and RAPD + ISSR combination, the populations under analysis were classified into 3, 3 and 5 groups, respectively. With this study, the closest populations were identified and a significantly high genetic diversity was detected.

scholarly journals Genetic diversity of chickpea genotypes as revealed by ISSR and RAPD markers

Genetika ◽  
2017 ◽  
Vol 49 (2) ◽  
pp. 415-423
Author(s):  
Saida Hasanova ◽  
Zeynal Akparov ◽  
Alamdar Mammadov ◽  
Litfer Amirov ◽  
Sevda Babayeva ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Rapd Markers ◽  
Diversity Index ◽  
Issr Markers ◽  
Similarity Coefficient ◽  
Parental Lines ◽  
Spss Software ◽  
Rapd And Issr ◽  
Combined Data ◽  
Issr Primers

Genetic diversity of 62 chickpea accessions was studied using 8 ISSR and 11 RAPD primers. In the study RAPD primers detected more polymorphism (98%) than the ISSR primers (80%). Genetic diversity index was high (0.73 for ISSR and 0.85 for RAPD) for each of these marker systems. Cluster analysis performed from both separate and combined data of RAPD and ISSR markers using SPSS software package. Jaccard?s similarity coefficient for 62 chickpea genotypes was 0.65. Cluster analyses based on combined data generated a dendrogram that separated genotypes into 11 clusters. Four clusters contained only one genotype showing the genetic uniqueness of these accessions. The studied chickpea collection has been proved to constitute a rich source of biodiversity as revealed by RAPD and ISSR markers. Crossing between distantly related genotypes is expected to yield more vigorous plants constituting much of the different traits contained in the two parental lines.

scholarly journals Determination of Camellia oleifera Abel. Germplasm Resources of Genetic Diversity in China using ISSR Markers

2018 ◽  
Vol 46 (2) ◽  
pp. 501-508
Author(s):  
Junyong CHENG ◽  
Dezhi JIANG ◽  
Hua CHENG ◽  
Xihua ZHOU ◽  
Yingyou FANG ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Relationships ◽  
Issr Markers ◽  
Germplasm Management ◽  
Camellia Oleifera ◽  
High Genetic Diversity ◽  
Upgma Cluster Analysis ◽  
Close Genetic Relationship ◽  
Issr Primers

Camellia oleifera is one of the four woody oil plants in the world, which is widely cultivated in South China. To examine the genetic diversity of C. oleifera in China, the diversity and genetic relationships among and within major populations of 109 varieties of C. oleifera were analyzed using ISSR markers. Twenty-three ISSR primers out of 49 primers yielded approximately 487 legible bands. A total of 335 of these bands were polymorphic markers, and the ratio of polymorphism was 68.86%. From the results, Zhejiang province showed the highest populations genetic diversity (H value 0.18), while Guangxi population showed the lowest genetic diversity (H 0.0851). Base on the bands, the genetic similarity coefficient ranged from 0.61 to 0.93 using NTSYS2.10e software. When coefficient was 0.75, 109 cultivars were divided into 11 categories and categories I contain 79 varieties by UPGMA cluster analysis. The test varieties divided into 7 sub-groups when categories were 0.75, which show a close genetic relationship. Results advised that Hunan is the main producing area of C. oleifera, with enriched C. oleifera variety and complex topography, and therefore has a high genetic diversity. Meanwhile, the main varieties of C. oleifera in Hubei are imported from Hunan, which results in fewer varieties and reduces the genetic diversity of C. oleifera. The ISSR profiles can improve C. oleifera germplasm management and provide potential determine correlations between different varieties and its distribution in different province.

scholarly journals Morphological and Molecular Characterization of Iranian Wild Blackberry Species Using Multivariate Statistical Analysis and Inter-Simple Sequence Repeats (ISSR) Markers 

2021 ◽  
Author(s):  
Ali Gharaghani ◽  
Mehdi Garazhian ◽  
Saeid Eshghi ◽  
Ahmad Tahmasebi
Keyword(s):  
Genetic Diversity ◽  
Simple Sequence Repeats ◽  
Morphological Traits ◽  
Diversity Index ◽  
Issr Markers ◽  
Multivariate Statistical ◽  
Principal Coordinates ◽  
Inter Simple Sequence Repeats ◽  
Simple Sequence ◽  
Issr Primers

Abstract This study was carried out to estimate the genetic diversity and relationships of 74 Iranian blackberry genotypes assigned to 5 different species using inter-simple sequence repeats (ISSR) marker analysis and morphological trait characterization. Sixteen traits including phenological, vegetative and reproductive attributes were recorded, and 10 ISSR primers were screened. Results showed that yield and leaf width have the highest and lowest genetic diversity, (diversity index = 62.57 and 13.74), respectively. Flowering and ripening date recorded as traits having the strongest correlations (r = 0.98). The selected 10 ISSR primers produced a total of 161 amplified fragments (200 to 3500 bp) of which 113 were polymorphic. The highest, lowest and average PIC values were 0.53, 0.38 and 0.44, respectively. Principle component analysis (PCA) based on morphological traits showed that the first six components explained 84.9% of the variations of traits studied, whilst the principal coordinate analysis (PCoA) based on ISSR data implied the first eight principal coordinates explained 67.06% of the total variation. Cluster analysis based on morphological traits and ISSR data classified all genotypes into two and three major groups, respectively, and the distribution pattern of genotypes was mainly based on species and the geographic origins.

scholarly journals Genetic variability of lemon basil (Ocimum × africanum Lour.) from Indonesian archipelago based on morphological and inter-simple sequence repeats markers

2020 ◽  
Vol 21 (12) ◽  
Author(s):  
Kurnia Makmur ◽  
TATIK CHIKMAWATI ◽  
SOBIR SOBIR
Keyword(s):  
Genetic Diversity ◽  
Genetic Variability ◽  
Similarity Index ◽  
Morphological Characters ◽  
Molecular Data ◽  
Issr Markers ◽  
Morphological Observation ◽  
Indonesian Archipelago ◽  
Morphological And Molecular Data ◽  
Issr Primers

Abstract. Makmur K, Chikmawati T, Sobir. 2020. Genetic variability of lemon basil (Ocimum × africanum Lour.) from Indonesia based on morphological characters and ISSR markers. Biodiversitas 21: 5948-5953. Lemon basil (Ocimum × africanum Lour.) or kemangi is a highly beneficial plant, yet still underutilized. Therefore, providing necessary information on genetic diversity of this species is essential for further utilization. The aim of this study was to elucidate genetic diversity of 33 accessions of O. × africanum collected from four Islands of Indonesia along with three accessions of Ocimum basilicum L. Morphological observation was conducted on 37 morphological characters following the International Union for the Protection of New Varieties of Plants descriptor guidelines; subsequently, 13 ISSR primers were employed in molecular analysis. Both morphological and molecular data were analyzed based on simple matching similarity index using UPGMA method. Morphologically, O. × africanum and O. basilicum were clearly separated at the similarity index of 0.52, and among two species were divided into two groups according to two either character. ISSR analysis using 13 ISSR primers produced 111 DNA bands, and 108 of them (97.29%) were polymorphic. Cluster analysis based on ISSR data could not explicitly separate O. × africanum and O. basilicum accessions. Besides, Ocimum accessions collected from the same area did not always cluster into one group.

scholarly journals Genetic diversity and relationships among salvia species by ISSR markers

Genetika ◽  
2021 ◽  
Vol 53 (2) ◽  
pp. 559-574
Author(s):  
Zhao Yanpeng ◽  
Wang Hongmei ◽  
Liang Wei ◽  
Majid Khayatnezhad ◽  
F Faisal
Keyword(s):  
Genetic Diversity ◽  
Conservation Biology ◽  
Issr Markers ◽  
High Genetic Diversity ◽  
Marker Index ◽  
Relationship Analysis ◽  
Inter Simple Sequence Repeats ◽  
Issr Molecular Markers ◽  
Simple Sequence ◽  
Issr Primers

Species identification is fundamentally important within the fields of conservation, biology, biogeography and ecology. Salvia species are herbaceous, biennial or annual, strongly aromatic. Inter-Simple sequence repeats (ISSR) molecular markers were used for evaluate genetic diversity and relationship analysis of 30 Salvia species. Ten selected ISSR primers amplified 116 loci, respectively, of which all were polymorphic. The obtained average polymorphism information content 0.39, average band informativeness 10.5 and the marker index 3.1 revealed high genetic diversity prevailing among Salvia accessions. The dendrogram was constructed based on ISSR separated the individuals into sub-clusters in accordance with their species. Our results indicated that ISSR markers can be used as a reliable and informative technique for evaluation of genetic diversity and relationships among Salvia species. The objectives of present study are: 1) can ISSR markers identify Salvia species, 2) what is the genetic of these taxa in Iran, and 3) to investigate the species inter-relationship?

scholarly journals Molecular analysis of genetic diversity in population of Silybum marianum (L.) Gaertn in Egypt

2019 ◽  
Vol 17 (1) ◽  
Author(s):  
Marwa Hamouda
Keyword(s):  
Genetic Diversity ◽  
Electrophoretic Pattern ◽  
Issr Markers ◽  
Silybum Marianum ◽  
Inter Simple Sequence Repeats ◽  
Sds Page ◽  
Dna Pattern ◽  
Pharmaceutical Properties ◽  
High Level ◽  
Rapd And Issr

Abstract Background Silybum marianum L. Gaertn is a medicinal plant of unique pharmaceutical properties in the treatment of liver disorders and diabetic nephropathy. Biochemical (SDS-PAGE) and molecular markers such as randomly amplified polymorphic DNA (RAPD) and inter-simple sequence repeats (ISSR) technologies were used in this work to detect genetic diversity of 14 collections of Silybum marianum population in Egypt. Results The electrophoretic pattern of seed protein gave different molecular weight bands, ranging from 24 to 111 KDa with the presence of unique bands. RAPD results revealed a high level of polymorphism (73.2%) using 12 RAPD primers, but only eight of them gave reproducible polymorphic DNA pattern. Sixteen primers were used in the ISSR method; only ten of them yielded clearly identifiable bands. The percentage of polymorphism is about 80% of the studied samples. Conclusion The obtained data confirmed that SDS-protein, RAPD, and ISSR markers are important tools for genetic analysis for Silybum marianum and recommended to give accurate results.

scholarly journals Efficiency of improved RAPD and ISSR markers in assessing genetic diversity and relationships in Angelica sinensis (Oliv.) Diels varieties of China

2015 ◽  
Vol 18 (2) ◽  
pp. 96-102 ◽  
Author(s):  
Zhiqiang Mei ◽  
Chun Zhang ◽  
Md. Asaduzzaman Khan ◽  
Ye Zhu ◽  
Mousumi Tania ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Issr Markers ◽  
Angelica Sinensis ◽  
Rapd And Issr

Genetic Diversity among Calamus brandisii Becc. (Arecaceae) Populations in Kerala Part of Western Ghats

2021 ◽  
Vol 43 (1) ◽  
pp. 38-42
Author(s):  
Kavungal Priya ◽  
◽  
Indira . ◽  
Vadakkethil Balakrishnan Sreekumar ◽  
Renuka . ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Western Ghats ◽  
Rapd Markers ◽  
Gene Diversity ◽  
Issr Markers ◽  
High Genetic Diversity ◽  
Western Ghats Of India ◽  
The Mean ◽  
Two Parameters ◽  
Two Populations

Calamus brandisii Becc. is one of the endemic slender rattans found in the Western Ghats of India. The genetic diversity of two main populations available in Kerala was investigated using 20 RAPD and 9 ISSR markers. Two parameters viz., gene diversity and genetic diversity within and among populations were analyzed. ISSR analysis showed quite high genetic diversity in Pandimotta compared to Bonacaud population whereas in RAPD markers both these populations were moderately diverse. The percentage of total genetic differentiation (Gst) among two populations is relatively higher than the mean Gst value indicating high genetic diversity within the populations. The genetic distance between these two populations was 0.1739 with ISSR markers and 0.1971 with RAPD markers. Because of its high genetic diversity, Pandimotta population can be treated as an important population of gene diversity with potentially useful genes. This may be included in the high priority reservoir for genetic conservation also.

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