scholarly journals RAMAN FLUORESCENCE CHARACTERISTICS OF HARD DENTAL TISSUES AND THEIR CLINICAL SIGNIFICANCE

Author(s):  
Mikhail Timofeevich Aleksandrov ◽  
V. I Kukushkin ◽  
M. A Polyakova ◽  
N. E Novozhilova ◽  
K. S Babina ◽  
...  

The aim of the study is to study the spectral characteristics of the hard tissues of the tooth, their Raman-fluorescent components (RFS) and to determine the possibility of using the method to assess the degree of mineralization of hard tissues of the tooth. In a preclinical study on model test objects of different functional groups of teeth (incisors, premolars, molars), remote by clinical indications, Raman-fluorescence spectroscopy was performed using the laser hardware-software complex “Inspector M” with a wavelength of probing radiation 514 nm. In the course of the study, a qualitative and quantitative analysis of the content and distribution of hydroxyapatite in the structures of hard dental tissues (enamel, dentin, cement) in normal and pathological conditions was performed. The high sensitivity and expressiveness of the method, the possibility of quantitative processing of the results of the study, which allows us to recommend it for assessing the degree of mineralization/demineralization of the tooth, the effectiveness of remineralizing drugs and methods of their use in the clinic of therapeutic dentistry.

1990 ◽  
Vol 69 (10) ◽  
pp. 1669-1673 ◽  
Author(s):  
W. Beertsen ◽  
V. Everts

After the periodontium of the rat was wounded, the formation of acellular extrinsic fiber cementum (AEFC) did not appear to be restricted to the hard dental tissues (pre-existing cementum, dentin, and enamel). Layers resembling AEFC were also deposited along the inner wall of the alveolar bone. At the time of observation (six weeks after being wounded), cells other than fibroblast-like cells could not be distinguished close to the newly formed AEFC-like layers. We suggest that the deposition of this material was related to the activity of periodontal ligament fibroblasts.


Author(s):  
Mikhail Timofeevich Alexandrov ◽  
E. F Dmitrieva ◽  
A. N Akhmedov ◽  
O. A Artemova ◽  
A. Potrivailo ◽  
...  

The aim of the study is to study the state of mineralization of various anatomical and topraphic zones of teeth for different functiona I groups and to justify its clinical feasibility. In a preclinical in vitro study on 20 model test objects of teeth (incisors, canines, premolars and painters), removed according to clinical indications, Raman-fluorescent study of the degree of mineralization of various anatomical and topographic zones of the tooth in different functional groups of teeth was carried out. Used APC Inspector M with a wavelength of532 nm probing radiation. The advantages of Raman fluorescence spectroscopy for determining the degree of mineralization of hard tooth tissues are objectivity (digital technology), expressiveness, non-invasiveness, simple and non-destructive control of the degree of mineralization/remineralization of hard tooth tissues, the ability to document and store information. In th e course of the study, a qualitative and quantitative analysis of the mineralization of various anatomical and topraphic zones of teeth for different functional groups of teeth was carried out. High sensitivity and reproducibility of the method allowed to reveal significant differences in mineralization of tooth enamel in the area of the cutting edge, equator and neck of the tooth. It is shown that these differences are manifested in other functional groups of teeth (p < 0.05).


Materials ◽  
2021 ◽  
Vol 14 (10) ◽  
pp. 2558
Author(s):  
Mihaela Olaru ◽  
Liliana Sachelarie ◽  
Gabriela Calin

With the development of the modern concept of tissue engineering approach and the discovery of the potential of stem cells in dentistry, the regeneration of hard dental tissues has become a reality and a priority of modern dentistry. The present review reports the recent advances on stem-cell based regeneration strategies for hard dental tissues and analyze the feasibility of stem cells and of growth factors in scaffolds-based or scaffold-free approaches in inducing the regeneration of either the whole tooth or only of its component structures.


2016 ◽  
Vol 40 (2) ◽  
pp. 152-155 ◽  
Author(s):  
TE Shishniashvili ◽  
NN Suladze ◽  
VV Margvelashvili

Objectives: To study the influence of environmental pollution on the mineralization of dental hard tissues by using biosubstrates: teeth and hair. :Study design: At the first stage epidemiological survey was conducted in polluted and less polluted areas of Tbilisi (Georgia). We studied 525 children aged 3 and 4 years. Caries prevalence and intensity was defined by the methodology of World Health Organization. At the second stage the chemical elements content was studied in hair and teeth hard tissues of 24 children by X - ray fluorescent spectroscopy method. Results: The prevalence of dental caries in the polluted region was 46%, caries intensity − 1.92 (± 2.842). In the less polluted region prevalence was 37%, caries intensity − 1.47 (± 2.571). These data are statistically reliable (p &lt; 0.05). The study of hair and tooth tissues showed that the toxic elements (Pb, Hg, Sn, Ti) content in these tissues was higher in environmentally unfavorable than in favorable conditions. Conclusion: Hair and dental tissues can be used as indicators of environmental pollution. Our survey showed that toxic elements content in dental hard tissues was higher and the level of essential elements was less in polluted than in less polluted areas of Tbilisi.


Author(s):  
G. A. Loban ◽  
T. O. Petrushanko ◽  
V. V. Chereda ◽  
M. O. Faustova ◽  
M. M. Ananieva ◽  
...  

Background. Periodontal tissues inflammatory diseases are widespread among young people. Objective. This study was aimed at elaborating the method to assess risks of periodontal inflammatory diseases and determining its efficacy depending on the state of dental tissues, gum tissues and sex.          Methods. The study included 182 students (93 men, 89 women) aged 19-29: 22 individuals had no lesions of hard dental tissues and no signs of periodontal disease; 51 individuals were found to have DMF index <6; 52 individuals – DMF index ≥6; 57 individuals were diagnosed with chronic catarrhal gingivitis. Primary groups were formed in autumn; re-examination was carried in spring. The research participants were assessed for detection of risks of periodontal inflammatory disease by the method developes by the authors (Patent UA 54041). Results. The study revealed that the risk of development of preiodontitis increases in individuals with high caries and gingivitis intensity. In spring, more individuals suffer from microbial imbalance in in the composition of gingival sulcus fluid and decrease in the mean stability coefficient value that indicates an increased risk of inflammatory periodontal disease development. Women were less likely to experience seasonal dysbiotic changes in the gingival sulcus fluid composition compared with men. Conclusions. The method suggested for assessment of the risk of periodontal inflammatory diseases is of high informativeness. It allows clinicians detecting early pre-nosological signs of oral microbiocenosis imbalance that enhances the effectiveness of early diagnosis of inflammatory periodontal diseases.


Author(s):  
Jianwen Ma ◽  
Haihao Cheng ◽  
Xuemei Yang ◽  
Songyang Zhang ◽  
Yongqi Li ◽  
...  

2016 ◽  
Vol 37 (2) ◽  
pp. 401-413 ◽  
Author(s):  
Vedran Šantak ◽  
Alenka Vesel ◽  
Rok Zaplotnik ◽  
Marijan Bišćan ◽  
Slobodan Milošević

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