scholarly journals Sperm quality of Litopenaeus vannamei broostock injected by PMSG and antidopamin

2015 ◽  
Vol 14 (2) ◽  
pp. 98
Author(s):  
Fahmi Akbar ◽  
Agus Oman Sudrajat ◽  
Siti Subaidah
Keyword(s):  
Litopenaeus Vannamei ◽  
Sperm Quality ◽  
Sperm Count ◽  
White Shrimp ◽  
Hatching Rate ◽  
Eyestalk Ablation ◽  
Pregnant Mare Serum Gonadotropin ◽  
Abnormal Sperm ◽  
Serum Gonadotropin

<p class="NoParagraphStyle" align="center"><strong>ABSTRACT</strong><strong></strong></p><p class="NoParagraphStyle" align="center"><strong> </strong></p><p class="NoParagraphStyle">The important role in determining the productivity of shrimp was the quality and quantity of shrimp sperm. The decreasing of hatching rate was predicted as the effect of the decreasing quality of sperm. It then could  influence  the number and quality of naupli produced. Hormonal induction of maturation is one of alternative solution that can improve shrimp sperm quality. This study was conducted to examine the effect of pregnant mare serum gonadotropin (PMSG) and antidopamine (AD) injection on white shrimp <em>Litopenaeus vannamei</em> sperm quality. This research consisted of six treatments which were treatment without eyestalk ablation, eyestalk ablation, and premix PMSG hormone, and AD at the dose  of 0.1 mL/kg, 0.25 mL/kg, 0.5 mL/kg, and 1 mL/kg. The observed parameters were sperm count and percentage of normal and abnormal sperm. The results showed that PMSG hormone and AD injection could improve sperm quality of <em>L. vannamei</em> shrimp. Hormone at the dose of 0.25 mL/kg and 0.5 mL/kg were the optimal doses to increase sperm count and the percentage of normal sperm, also to lower the percentage of abnormal sperm.</p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle">Keyword: PMSG, AD, sperm quality, <em>Litopenaeus vannamei</em></p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle" align="center"><strong>ABSTRAK</strong></p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle">Kuantitas dan kualitas sperma udang jantan sangat berperan penting dalam menentukan produktivitas udang. Terjadinya penurunan daya tetas telur udang diduga karena terjadinya penurunan kualitas sperma. Hal ini berpengaruh terhadap jumlah dan kualitas nauplius yang diproduksi. Induksi maturasi secara hormonal merupakan salah satu alternatif yang dapat meningkatkan kualitas sperma udang. Penelitian ini dilakukan untuk mengkaji pengaruh penyuntikan <em>pregnant mare serum gonadotropin</em> (PMSG) dan antidopamin (AD) terhadap kualitas sperma udang vaname <em>Litopenaeus vannamei</em>. Penelitian terdiri atas enam perlakuan, yaitu perlakuan tanpa ablasi mata, ablasi mata, dan injeksi dengan premix hormon PMSG dan AD dosis 0,1 mL/kg, 0,25 mL/kg, 0,5 mL/kg, dan 1 mL/kg. Parameter yang diamati jumlah sperma, persentase sperma normal dan abnormal. Hasil penelitian menunjukkan penyuntikan hormon PMSG dan AD dapat meningkatkan kualitas sperma udang <em>L. vannamei</em>. Hormon dosis 0,25 mL/kg dan 0,5 mL/kg merupakan dosis optimal dalam meningkatkan jumlah sperma dan persentase sperma normal, serta mengurangi persentase sperma abnormal.</p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle">Kata kunci: PMSG, AD, kualitas sperma, <em>Litopenaeus vannamei</em></p><p> </p>

scholarly journals Supplementation of astaxanthin and vitamin E in feed on the development of gonads white shrimp broodstock Litopenaeus vannamei Boone 1931

2017 ◽  
Vol 16 (2) ◽  
pp. 135
Author(s):  
Fajar Maulana ◽  
Harton Arfah ◽  
Mita Istifarini ◽  
Mia Setiawati
Keyword(s):  
Growth Rate ◽  
Vitamin E ◽  
Litopenaeus Vannamei ◽  
Relative Survival ◽  
White Shrimp ◽  
Hatching Rate ◽  
High Quality ◽  
Gonad Maturity ◽  
Vitamin E Supplementation

<p class="Pa3"><strong>ABSTRACT </strong></p><p> </p><p>Increase production high quality of white shrimp <em>Litopenaeus vannamei</em> broodstock can be done with the addition of vitamin E or astaxanthin. A dose used was 350 ppm vitamin E, 500 ppm astaxanthin, combination of 350 ppm vitamin E, and 500 ppm astaxanthin, combination of 175 ppm vitamin E and 250 ppm astaxanthin. The purpose of this research was to evaluate the effect of astaxanthin and vitamin E supplementation with different dose in the feed on gonad maturity of white shrimp broodstock. Feeding of vitamin E and astaxanthin was performed in three times, at 06.00, 12.00 and 20.00 by as much as 2% of the shrimp weight. The results showed that combination of 175 ppm vitamin E and 250 ppm astaxanthin giving highest relative survival (100.00±0.00%), growth rate of broodstock shrimp (1.07±0.26%/day), the fastest maturity of first gonads level achieved 14 days (19.45 ±4.81%), maturity of fourth gonads level achieved 41 days, levels of spawning (33.33±8.33%), fecundity (87,000±2,000 eggs) and hatching rate (49±1.53%).</p><p> </p><p class="Pa5">Keywords: astaxanthin, <em>Litopenaeus vannamei</em>, vitamin E.</p><p> </p><p> </p><p class="Pa3"><strong>ABSTRAK </strong></p><p> </p><p class="Pa5">Meningkatkan kualitas induk udang vaname <em>Litopenaeus vannamei </em>dapat dilakukan dengan penambahan vitamin E atau astaxanthin pada pakan. Tujuan penelitian ini adalah untuk mengetahui pengaruh pemberian astaxanthin dan vitamin E dengan dosis berbeda dalam pakan terhadap tingkat kematangan gonad calon induk udang vaname. Dosis yang digunakan 350 ppm vitamin E, 500 ppm astaxanthin, 350 ppm vitamin E, dan 500 ppm astaxanthin, 175 ppm vitamin E dan 250 ppm astaxanthin. Pemberian pakan dengan penambahan vitamin E dan astaxanthin dilakukan sebanyak tiga kali, yaitu jam 06.00, 13.00, dan 20.00 WIB sebanyak 2% dari bobot udang. Hasil menunjukkan bahwa perlakuan terbaik didapatkan pada pemberian kombinasi 175 ppm vitamin E dan 250 ppm astaxanthin dengan tingkat sintasan induk udang 100,00±0,00%, laju pertumbuhan spesifik induk udang 1,07±0,26%/hari, kematangan gonad pertama tercepat dicapai pada 14 hari sebanyak 19,45±4,81%, kematangan gonad keempat didapat pada hari ke-41, tingkat pemijahan 33,33±8,33%, fekunditas 87.000±2.000 telur, dan derajat penetasan telur mencapai 49±1,53%.</p><p> </p><p>Kata kunci: astaxanthin, udang vaname <em>Litopenaeus vannamei</em>, vitamin E</p>

Effect of new dietary supplement on sperm quality

2012 ◽  
Vol 153 (45) ◽  
pp. 1787-1792 ◽  
Author(s):  
Mária Horváth ◽  
Endre Czeizel
Keyword(s):  
Adverse Effect ◽  
Clinical Trial ◽  
Dietary Supplement ◽  
Male Fertility ◽  
Sperm Quality ◽  
Sperm Count ◽  
Before And After ◽  
The Usa ◽  
New Treatments

Introduction: There is a decline in male fertility thus new treatments are needed. Aims: To test the efficacy of a new dietary supplement developed in the USA and registered as a curing drug in Hungary (OGYI). Methods: In a clinical trial 100 men with low sperm quality (spermium count 5–20 M/ml, good motility 10–40%, and adverse shape 30–50%) were examined. Results: Sperm parameters were measured before and after a 3-month treatment and after another 3-month without treatment. This dietary supplement statistically and clinically significantly improved sperm count and motility. In 74 cases this dietary supplement demonstrated a beneficial effect on sperm quality (more than 10% increase in sperm count, or quality of motility, or shape); in 16 cases the improvement exceeded 30%. No adverse effect could be accounted for this treatment. Conclusions: This new dietary supplement may contribute to the treatment of male infertility. Orv. Hetil., 2012, 153, 1787–1792.

scholarly journals Effect of Cell-Free Supernatant from Aeromonas sobria on the Spoilage of Shewanella putrefaciens in Pacific White Shrimp (Litopenaeus vannamei) with the Influence of Temperature Fluctuation

2019 ◽  
Vol 9 (3) ◽  
pp. 587 ◽  
Author(s):  
Jing-Xin Ye ◽  
Sheng-Ping Yang ◽  
Yun-Fang Qian ◽  
Jing Xie
Keyword(s):  
Litopenaeus Vannamei ◽  
Temperature Fluctuation ◽  
Experimental Period ◽  
Shewanella Putrefaciens ◽  
Pacific White Shrimp ◽  
Cold Chain ◽  
White Shrimp ◽  
Cell Free Supernatant ◽  
Aeromonas Sobria

The objective of this study was to evaluate the effect of cell-free supernatant (CFS) from Aeromonas sobria on the growth and spoilage potential of Shewanella putrefaciens in Pacific white shrimp (Litopenaeus vannamei) during cold chain logistics, including transportation, retailing, and domestic storage. It was shown that the quality of shrimps deteriorated in the cold chain logistics over time. The temperature fluctuation during the experimental period favored the growth of S. putrefaciens, increased the total volatile basic nitrogen (TVB-N) and biogenic amine value, and decreased the sensory quality of shrimps. The application of CFS resulted in the decline on the growth of S. putrefaciens after the early stationary phase stored at a cold condition. It is concluded that the application of CFS can inhibit microbial growth and the spoilage potential of S. putrefaciens and offset the quality deterioration of shrimp exposed to temperature fluctuation during cold chain logistics.

scholarly journals O papel do dimetilsulfóxido na criopreservação de sêmen de Curimba (Prochilodus lineatus)

2015 ◽  
Vol 36 (5) ◽  
pp. 3471
Author(s):  
Antonio Sergio Varela Junior ◽  
Estela Fernandes Silva ◽  
Tainã Figueiredo Cardoso ◽  
Érica Yokoyama Namba ◽  
Rodrigo Desessards Jardim ◽  
...  
Keyword(s):  
Sperm Quality ◽  
Membrane Integrity ◽  
Fertilization Rate ◽  
Dna Integrity ◽  
Hatching Rate ◽  
Sperm Viability ◽  
Prochilodus Lineatus ◽  
Dmso Concentration ◽  
Fresh Semen

<p class="Pa7">Cryopreservation of Curimba semen (Prochilodus lineatus) is ecological and commercial importance. The objective of this study was to evaluate the effect of different concentrations (2, 5, 8 and 11%) of dimethyl sulfoxide (DMSO) diluted in Betsville Thawing Solution (BTS) on the quality of post-thaw semen Curimba. We analyzed the rate and period motility, sperm viability, membrane integrity and DNA, mitochondrial functionality, and fertilization and hatching rate. The plasma membrane and DNA integrity of a DMSO concentration of 11% obtained better results than the concentration of 5% (p &lt;0.05). However, treatment of 5% DMSO resulted in a longer latency and a higher fertilization rate and hatching, in other sperm quality equal to that of fresh semen. The results of this study indicate that 5% DMSO is ideal for cryopreservation of semen Curimba.</p>

Sperm Quality Over Consecutive Spermatophore Regenerations in the Pacific White Shrimp Litopenaeus vannamei

2004 ◽  
Vol 35 (2) ◽  
pp. 178-188 ◽  
Author(s):  
Bertha P. Ceballos-Vázquez ◽  
Benjamin Aparicio-Simón ◽  
Elena Palacios ◽  
Ilie S. Racotta
Keyword(s):  
Litopenaeus Vannamei ◽  
Sperm Quality ◽  
Pacific White Shrimp ◽  
White Shrimp ◽  
The Pacific

Dietary Effects on Sperm Quality of Litopenaeus vannamei Broodstock

2003 ◽  
Vol 34 (1) ◽  
pp. 92-98 ◽  
Author(s):  
Martin Perez-Velazquez ◽  
Mayra L. Gonzáez-Félix ◽  
Addison L. Lawrence ◽  
William A. Bray ◽  
Delbert M. Gatlin
Keyword(s):  
Litopenaeus Vannamei ◽  
Sperm Quality ◽  
Dietary Effects

scholarly journals Supplementation of astaxanthin and vitamin E in feed on the development of gonads white shrimp broodstock Litopenaeus vannamei Boone 1931

2017 ◽  
Vol 16 (2) ◽  
pp. 135
Author(s):  
Fajar Maulana ◽  
Harton Arfah ◽  
Mita Istifarini ◽  
Mia Setiawati
Keyword(s):  
Growth Rate ◽  
Vitamin E ◽  
Specific Growth Rate ◽  
Litopenaeus Vannamei ◽  
Specific Growth ◽  
Pacific White Shrimp ◽  
White Shrimp ◽  
Optimum Dose ◽  
Hatching Rate ◽  
Gonad Maturity

<p class="NoParagraphStyle"><strong>ABSTRACT</strong></p><p class="NoParagraphStyle"><strong> </strong></p><p class="NoParagraphStyle">The quality of white shrimp <em>Litopenaeus vannamei</em> broodstock can be improved through the addition of astaxanthin and vitamin E in the diet. This study aimed to determine the effect of administration of astaxanthin and vitamin E with different doses in the feed on the maturity gonad of prospective Pacific white shrimp broodstock. Supplementation of 0 mg/kg feed astaxanthin + 0 mg/kg feed vitamin E (control/A),  500 mg/kg feed astaxanthin (B),  350 mg/kg  feed vitamin E (C), 500 mg/kg feed astaxanthin and 350 mg/kg feed vitamin E (D), and  250 mg/kg feed astaxanthin and 175 mg/kg feed vitamin E (E) were applied in feed formulation. Shrimp was fed 2% of body weight three times daily at 06.00 am, 13.00 pm, and 20.00 pm. The result showed that the optimum dose for survival, specific growth rate and maturity level of Pacific white shrimp broodstock was obtained in the combination of 175 mg/kg vitamin E and 250 mg/kg astaxanthin. The  survival of shrimp by that treatment was 100.00±0.00%, specific growth rate 1.07±0.26%/day, the first level of gonad maturity growth was reached at day 14 (19.45±4.81%), the fourth level of gonad maturity was obtained at day 41, spawning rate 33.33±8.33%, fecundity 87,000±2,000 eggs, and hatching rate reached 49.00±1.53%.</p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle">Keywords: astaxanthin, <em>Litopenaeus vannamei</em>, vitamin E</p><p class="NoParagraphStyle"><strong> </strong></p><p class="NoParagraphStyle"><strong> </strong></p><p class="NoParagraphStyle"><strong>ABSTRAK</strong><strong></strong></p><p class="NoParagraphStyle"><strong> </strong></p><p class="NoParagraphStyle">Peningkatan kualitas induk udang vaname <em>Litopenaeus vannamei </em>dapat dilakukan dengan penambahan vitamin E dan astaxanthin pada pakan. Tujuan penelitian ini adalah untuk mengevaluasi pengaruh pemberian astaxanthin dan vitamin E dengan dosis berbeda dalam pakan terhadap tingkat kematangan gonad calon induk udang vaname. Dosis yang digunakan adalah 0 mg/kg pakan astaxanthin + 0 mg/kg pakan vitamin E (kontrol/A),  500 mg/kg pakan astaxanthin (B),  350 mg/kg  pakan vitamin E (C), 500 mg/kg pakan astaxanthin and 350 mg/kg pakan vitamin E (D), and  250 mg/kg pakan astaxanthin and 175 mg/kg pakan vitamin E (E). Pemberian pakan dengan penambahan vitamin E dan astaxanthin dilakukan sebanyak tiga kali, yaitu jam 06.00, 13.00, dan 20.00 WIB sebanyak 2% dari bobot udang. Hasil penelitian menunjukkan bahwa dosis optimum untuk sintasan, laju pertumbuhan spesifik, dan tingkat kematangan induk udang vaname diperoleh dengan kombinasi 175 mg/kg vitamin E dan 250 mg/kg astaxanthin. Kelangsungan hidup udang dengan perlakuan tersebut adalah 100,00±0,00%, laju pertumbuhan spesifik 1,07±0,26%/hari, tingkat kematangan gonad pertama dicapai pada hari ke 14 (19,45±4,81%), tingkat kematangan gonad keempat diperoleh pada hari ke 41, tingkat pemijahan 33,33±8,33%, fekunditas 87.000±2.000 telur, dan tingkat penetasan mencapai 49,00±1,53%. </p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle">Kata kunci: astaxanthin, udang vaname <em>Litopenaeus vannamei</em>, vitamin E</p>

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