scholarly journals Grouper nursery development in sea floating net cage through the application of Phyllanthus niruri-Allium sativum flour in feed

2021 ◽  
Vol 20 (2) ◽  
pp. 191-202
Author(s):  
Irzal Effendi ◽  
Ahmad Trio Pratama ◽  
Aldy Mulyadin ◽  
Dinamella Wahjuningrum
Keyword(s):  
Allium Sativum ◽  
Phyllanthus Niruri ◽  
Net Cage

Untuk mendukung usaha budidaya ikan kerapu kini tengah digalakkan sistem pendederan intensif dalam keramba jaring apung (KJA) di laut. Salah satu cara untuk menjaga kondisi ikan tetap sehat dalam sistem tersebut yaitu dengan pemberian fitofarmaka seperti meniran-bawang putih. Tujuan penelitian ini adalah untuk mengevaluasi penambahan tepung meniran-bawang putih melalui pakan terhadap status kesehatan dan kinerja produksi benih ikan kerapu cantang pada pendederan dalam keramba jaring apung di laut. Benih ikan kerapu cantang (panjang 8.27 ± 0.16 cm dan bobot 10.89 ± 0.83 g) dipelihara dalam KJA berupa waring 1x1x1.5 m3 dan diberi pakan dengan penambahan tepung meniran-bawang putih 20 + 25 g/kg pakan selama 7 hari dan 14 hari pertama pemeliharaan, serta tanpa tepung meniran-bawang putih (kontrol) sebagai perlakuan. Ikan dipelihara selama 42 hari dan disampling setiap 2 minggu untuk diambil darah serta diukur bobot dan panjangnya. Penambahan tepung meniran-bawang putih selama 14 hari pada pakan ikan kerapu dapat meningkatkan status kesehatan dan kinerja produksi.   Kata kunci: ikan kerapu cantang, kinerja produksi, meniran-bawang putih, status kesehatan.

scholarly journals Combination of garlic - shatterstone herb powder to control Streptococcus agalactiae infection in tilapia

2015 ◽  
Vol 14 (1) ◽  
pp. 79
Author(s):  
Ririn Nurul Fauziah ◽  
Dinamella Wahjuningrum ◽  
, Sukenda ◽  
, Ranta
Keyword(s):  
Growth Rate ◽  
Streptococcus Agalactiae ◽  
Allium Sativum ◽  
Positive Control ◽  
Quality Parameters ◽  
Negative Control ◽  
Fish Pathogen ◽  
Phyllanthus Niruri

<p class="NoParagraphStyle" align="center"><strong>ABSTRACT</strong><strong></strong></p><p class="NoParagraphStyle" align="center"><strong> </strong></p><p class="NoParagraphStyle">This study was aimed at determining potential of combination powder of garlic <em>Allium sativum</em>-shatterstone herb <em>Phyllanthus niruri</em> supplemented in feed against <em>S. agalactiae</em> infection in tilapia. Four concentrations of combination powder of <em>A. sativum</em>-<em>P. Niruri</em>; 20+5, 20+10, 20+15 and 20+20 ppt respectively were investigated for their ability to inhibit bacterial fish pathogen. Combination dose of 20+15 ppt produced the highest inhibitory zones in <em>in vitro</em> test. <em>In vivo</em> test consisted of three treatments with three replications, namely positive control (K+), negative control (K-) and the treatment of <em>A. sativum</em>-<em>P. niruri</em> suplemented in feed (BM).  The test perfomed on tilapia with weight of 10.33 ± 1.63 g and were reared at density of 10 ind/aquarium. The fish was fed for 14 days, then injected intraperitoneally with 0.1 mL <em>S. agalactiae</em> at concentration of 10<sup>5</sup> cfu/mL for positive control and BM groups. Survival, growth rate, feed response, hematological and water quality parameters were observed for 10 days. This study showed that the suplemented-feed-fish (BM) showed better growth rate, feed response, and survival (83.3%) than positive control (36.7%) at P&lt;0.05. In addition, <em>A. sativum</em>-<em>P. niruri</em> suplemented in feed was also able to enhance the immune response by increasing phagocytic activity.</p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle">Keywords: <em>Streptococcus agalactiae</em>, phytopharmacy, <em>Allium sativum</em>-<em>Phyllanthus niruri</em>, tilapia</p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle" align="center"><strong>ABSTRAK</strong></p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle">Penelitian ini bertujuan untuk menganalisis potensi campuran tepung bawang putih <em>Allium sativum</em>-meniran <em>Phyllanthus niruri </em>dalam pakan terhadap pencegahan infeksi bakteri <em>S. agalactiae</em> pada ikan nila. Empat konsentrasi campuran tepung bawang putih-meniran yaitu 20+5 ppt, 20+10 ppt, 20+15 ppt dan 20+20 ppt masing-masing diuji kemampuannya dalam  menghambat bakteri patogen pada ikan. Campuran dosis 20+15 ppt menghasilkan zona hambat terbaik dalam uji <em>in vitro</em>. Uji <em>in vivo</em> terdiri atas tiga perlakuan dengan tiga ulangan yaitu kontrol positif, kontrol negatif, dan perlakuan pakan yang mengandung bawang putih-meniran (BM). Uji ini dilakukan pada ikan nila berbobot 10,33±1,63 g yang dipelihara di akuarium dengan kepadatan 10 ekor/akuarium. Ikan diberi pakan perlakuan selama 14 hari kemudian diinjeksi secara intraperitoneal dengan bakteri <em>S. agalactiae</em> sebanyak 0,1 mL dengan kepadatan 10<sup>5 </sup>cfu/mL pada perlakuan kontrol positif dan perlakuan BM. Parameter kelangsungan hidup, laju pertumbuhan, respons pakan, parameter hematologi, dan kualitas air diamati selama sepuluh hari. Hasil dari penelitian ini menunjukkan bahwa pemberian BM dalam pakan memberikan laju pertumbuhan, respons pakan, dan sintasan (83,3%) yang lebih baik daripada kontrol positif (36,7%) pada P&lt;0,05. Pakan yang mengandung campuran bawang putih-meniran ini juga mampu meningkatkan respons imun dengan adanya peningkatan aktivitas fagositosis.</p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle">Kata kunci: <em>Streptococcus agalactiae</em>, fitofarmaka, <em>Allium sativum</em>-<em>Phyllanthus niruri</em>, ikan nila</p><p> </p>

scholarly journals Utilization Of Nano Ethanolic Extract Combination Chamber Bitter (Phyllanthus Niruri L.) And Garlic (Allium Sativum L.) As A Natural Immunomodulator In Nanoherbal Development, In Silico And In Vitro Study

2017 ◽  
Vol 2 (02) ◽  
pp. 110
Author(s):  
Kadek Hendra Darmawan
Keyword(s):  
Molecular Docking ◽  
In Silico ◽  
Allium Sativum ◽  
In Vitro Study ◽  
Ethanolic Extract ◽  
Phagocytic Index ◽  
Phyllanthus Niruri ◽  
The Right ◽  
Phagocytosis Activity

The Filantin compounds in chamber bitter (<em>Phyllanthus niruri</em> L.) and lectin in garlic (<em>Allium sativum</em> L.) was proven as immunomudulatory agents through interaction with <em>Toll-Like Reseptors</em> (TLR) which have role in innate immune responds. Immunomodulators drug available on the market still have many shortcomings such as the low potential. Drug developing by nanotechnology is the right solution to increase the potential of the drug by increasing the absorption and minimize the dose. This research aimed to know the interaction of filantin and lectin with TLR2-TLR1 receptors through <em>molecular docking</em> and produce the nanoemulsion combination of chamber bitter and garlic ethanolic extracts that have phagocytosis activity. <em>In silico </em>assay through <em>molecular docking</em> showed that filantin has affinity for binding to TLR2-TLR1, docking score of lectin (-33,5389) was lower than the filantin (-31.5112). That means lectin has higher affinity for binding to TLR2-TLR1. Nanoemulsion was formulated by SNEDDS methods with composition of co-surfactant: surfactant: oil is 1: 5,25: 1. The nanoemulsion stable at 0,414% (w/v). <em>In vitro</em> assay of phagocytic index (5,03) and ratio (95%) showed that the formulation with nanoemulsion of the combination has higher phagocyte index and ratio than the formulation without nanoemulsion or even the positive controls.

Effect of co-administration of herbal extracts with copper nanoparticles: A novel two-pronged approach in treating type 2 diabetes

2020 ◽  
Vol 13 ◽  
Author(s):  
Saurabh Singh ◽  
Sachin Kumar Singh ◽  
Bimlesh Kumar ◽  
Barinder Kaur ◽  
Rubiya Khursheed ◽  
...  
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Combination Therapy ◽  
Copper Nanoparticles ◽  
Allium Sativum ◽  
Aloe Vera ◽  
Diabetic Rats ◽  
High Dose ◽  
Phyllanthus Niruri

Aim: Evaluation of antidiabetic potential of polyherbal formulation containing copper nanoparticles. Background: Copper, popularly known as “Tamra”, is well known for its use as Bhasma (incinerated powder) in Ayurveda herbal preparations in Indian traditional system of medicine. Further, Trigonella foenum- graecum, Allium sativum, Aloe vera and Phyllanthus niruri leaves are extensively cultivated in all parts of India and are well known to possess antidiabetic and antihyperlipidemic properties. Objective: The objective of present study is to investigate the effect of administration of incinerated copper nanoparticle alone, polyherbal extracts of four herbs alone and combination of incinerated copper nanoparticles-polyherbal extracts on streptozotocin induced type 2 diabetic rats. Materials and Methods: Copper nanoparticles were prepared by media milling of incinerated copper powder using combination of Glycyrrhiza glabra and gum acacia as stabilizers. Aqueous extracts of Trigonella foenum seeds, Allium sativum bulbs, Aloe vera and Phyllanthus niruri leaves was prepared. The study was carried out for 28 days after drug administration. Blood glucose levels, body weight, haemoglobin, biochemical parameters of blood and antioxidant levels and histopathological parameters of liver, kidney and pancreas were studied. Results: The study revealed improvement in body weight and reduction in serum glucose level in rats that have been administered polyherbal extracts alone, copper nanoparticles alone and combination of polyherbal extract and copper nanoparticles as compared to those treated with streptozotocin only (diabetic rats). However, the response was found to be significantly less in case of rats of those groups which were given either the extracts alone or nanoparticles alone compared to that in the animals of groups that were administered a combination therapy. Significant improvement in all the responses was observed in rats treated with high doses of copper nanoparticles as compared to those with lower dose. Maximum improvement in body weight and reduction in blood glucose was found in case of rats which were administered with 670 mg/kg polyherbal extract (low dose) + 3.5 mg/kg copper nanoparticles (high dose). This was followed by rats which were given the lower dose of combination therapy. Values of total haemoglobin, triglycerides, HDL, total cholesterol, VLDL, SGOT, SGPT, ALP, serum creatinine content, urea, and antioxidant levels of rats of group IX were almost similar to that of normal rats and glibenclamide treated rats (control). Conclusion: The results from the study clearly indicated protective effect of co-administration of copper nanoparticles and polyherbal extracts for effective treatment of diabetes over copper nanoparticles and polyherbal extracts alone.

scholarly journals Infection control of Aeromonas hydrophila in catfish (Clarias sp.) using mixture of meniran Phyllanthus niruri and garlic Allium sativum in feed

2013 ◽  
Vol 9 (2) ◽  
pp. 93 ◽  
Author(s):  
Dinamella Wahjuningrum ◽  
Eka Hidayatus Solikhah ◽  
Tatag Budiardi ◽  
Mia Setiawati
Keyword(s):  
Infection Control ◽  
Aeromonas Hydrophila ◽  
Allium Sativum ◽  
Preventive Treatment ◽  
Curative Treatment ◽  
Phyllanthus Niruri ◽  
Natural Materials ◽  
Active Substances

<p><em>Motile aeromonad septicaemia </em>(MAS) is caused by the bacterium <em>Aeromonas hydrophila</em>. The use of plant (natural materials) such as meniran and garlic can be as an alternative way to inhibit the activity of <em>A. hydrophila</em> by their active substances which have potency as an antibacterial and immunostimulant. The aim of this study was to analyze the effectiveness of using a mixture of meniran and garlic in feed to control of <em>A. hydrophila</em> in catfish. There were split in two kind of doses namely, preventive (garlic:meniran=5 ppt:20 ppt) and curative (garlic:meniran=10 ppt:40 ppt). The preventive treatment was given for two weeks before challenging test. The curative treatment was performed on 2<sup>th</sup>-8<sup>th</sup> day after challenging test.  Challenging test was carried out by intramuscularly injecting of 0,1 mL <em>A</em>. <em>hydrophila </em>(10<sup>8</sup>cfu/ml)  into the fish. The results indicated that preventive treatment with a mixture of extracts 5 ppt meniran and 20 ppt garlic was more effective in preventing infection of <em>A. hydrophila</em> than curative treatment.</p> <p> </p><p>Keywords: <em>Phyllanthus</em><em> niruri, Allium sativum</em>, catfish, <em>Aeromonas</em><em> hydrophila</em>.</p><p> </p><p> </p><p align="center"><strong>ABSTRAK</strong></p> <p><strong> </strong>Penyakit MAS (<em>motile aeromonad septicaemia</em>) disebabkan oleh bakteri <em>Aeromonas hydrophila</em>. Bahan alami seperti meniran dan bawang putih dapat digunakan sebagai alternatif untuk menghambat aktivitas bakteri ini.  Zat aktif yang dimiliki bahan ini berpotensi sebagai antibakteri dan immunostimulan. Tujuan dari penelitian ini adalah untuk menganalisis efektivitas penggunaan campuran meniran dan bawang putih dalam pakan untuk pengendalian penyakit MAS pada ikan lele. Perlakuan dibagi menjadi dua dosis, yaitu pencegahan (bawang putih:meniran=5 ppt:20 ppt) dan pengobatan (bawang putih:meniran=10 ppt:40 ppt). Perlakuan pencegahan diberikan selama seminggu sebelum uji tantang. Perlakuan pengobatan dilakukan pada hari ke 2 hingga hari ke-8 setelah uji tantang. Uji tantang dilakukan dengan menyuntikkan secara intramuskuler 0,1 ml <em>A. hydrophila</em> (10<sup>8</sup>cfu/ml) ke ikan lele. Hasil penelitian menunjukkan bahwa perlakuan pencegahan dengan campuran ekstrak meniran 5 ppt dan bawang putih 20 ppt efektif dalam mencegah infeksi <em>A. hydrophila</em> dibandingkan dengan perlakuan pengobatan dengan campuran ekstrak meniran 10 ppt dan 40 ppt bawang putih.</p> <p> </p> Kata kunci: meniran, bawang putih, lele, <em>Aeromonas</em><em> </em><em>hydrophila</em>.<p> </p>

scholarly journals Duration of feed application of mixed powders of Phyllanthus niruri and Allium sativum for the prevention of Aeromonas hydrophila infection in catfish Clarias sp.

2013 ◽  
Vol 11 (2) ◽  
pp. 179
Author(s):  
Dinamella Wahjuningrum ◽  
Iis Widiani ◽  
Sri Nuryati
Keyword(s):  
Survival Rate ◽  
Experimental Design ◽  
Aeromonas Hydrophila ◽  
Allium Sativum ◽  
Phyllanthus Niruri ◽  
Random Design ◽  
And Control

<p>Motile Aeromonad Septicaemia (MAS) caused by Aeromonas hydrophila induced serious epidemics of disease in catfish Clarias sp. The purposed of this research was to determine optimum durations of feed application of mixed powders Phyllanthus niruri and Allium sativum to prevent MAS. Experimental design that used was Complete Random Design which consist of three treatments and three replications. This research tested on three different of durations of feed application of P. niruri and A. sativum, namely A (powder of P. niruri and A. sativum feed application during 21 days and infected with A. hydrophila 0,1 mL IP), B (powder of P. niruri and A. sativum feed application during 14 days and injected IP with A. hydrophila 0,1 mL), C (powder of P. niruri and A. sativum feed application during seven days and injected IP with A. hydrophila 0,1 mL), and control, namely K- (without P. niruri and A. sativum feed application and injected IP with PBS 0,1 mL) and K+ (without P. niruri and A. sativum powder application and injected IP with A. hydrophila 0,1 mL). The treatments was given for 21 days before challenging test, at 22th day test in vivo carried out by injecting A. hydrophila (108 CFU/mL) into the fish by intramuscular and observed for 10 days. The highest value of survival rate that consist in treatment K- was 100±0.00%, treatment A was 93.3±11.55%, and treatment B was 73.33±30.55%. While treatment K+ and C have the same survival rate that is, 26.67±11.55%. The application P. niruri and A. sativum powder during 21 days was optimum for preventing MAS in catfish.</p><p>Keywords: Phyllanthus niruri, Allium sativum, Aeromonas hydrophila, Clarias sp.</p>

1696: May Phyllanthus Niruri ( Uriston®) Affect the Efficacy of ESWL on Renal Stones? A Prospective, Randomised Short Term Study

2005 ◽  
Vol 173 (4S) ◽  
pp. 460-460
Author(s):  
Antonio Celia ◽  
Salvatore Micali ◽  
Sighinolfi Maria Chiara ◽  
Grande Marco ◽  
Di Pietro Corradino ◽  
...  
Keyword(s):  
Renal Stones ◽  
Short Term ◽  
Phyllanthus Niruri ◽  
Term Study ◽  
Short Term Study

Effects of garlic (Allium sativum L.) in postweaning pigs – a placebo controlled study

2019 ◽  
Author(s):  
H Ayrle ◽  
H Nathues ◽  
A Bieber ◽  
N Quander ◽  
M Durrer ◽  
...  
Keyword(s):  
Allium Sativum ◽  
Controlled Study

EFEKTIVITAS ANTIBAKTERI BAWANG PUTIH (Allium sativum L) SEBAGAI PENGAWET ALAMI PADA IKAN LELE DUMBO (Clarias gariephinus) SEGAR

2019 ◽  
Vol 14 (1) ◽  
pp. 26
Author(s):  
Dyah Anggraeni ◽  
Nurlela Nurlela
Keyword(s):  
Room Temperature ◽  
Allium Sativum ◽  
Research Result ◽  
Clarias Gariepinus ◽  
Storage Period ◽  
The Body ◽  
Garlic Extract ◽  
African Catfish ◽  
Natural Preservatives ◽  
Colony Counter

Background: Natural preservatives are compounds produced by natural ingredients that can suppress bacterial growth and development. Natural preservatives are carried out because most of the preservatives circulating are chemicals and unsafe for the body. One of the natural preservatives is by using garlic extract (Allium sativum L).  Objective: This study is aimed to determine the effectiveness of the antibacterial garlic (Allium sativum L) as a natural preservative in fresh African catfish (Clarias gariepinus).  Method: This research used the Pour Plate iroculation method. African catfish (Clarias gariepinus) which is soaked with garlic (Allium sativum L) with a concentration of 7%, 14% and 21% for 30 minutes, then the fish will be kept at room temperature with a storage period of 24 hours and 48 hours and calculated growth in bacterial numbers with the Colony counter.  Result: Based on the research result, it was found that garlic extract (Allium sativum L) can obstruct the effectiveness of antibacterial in African catfish (Clarias gariepinus) at a concentration of 14%.

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