scholarly journals Study of gonadotropin (GtH) stimulating hormone on gonad maturation of climbing perch Anabas testudineus Bloch

2010 ◽  
Vol 9 (1) ◽  
pp. 61
Author(s):  
. Suriansyah ◽  
M. Zairin Junior ◽  
Agus Oman Sudrajat
Keyword(s):  
Body Weight ◽  
Key Words ◽  
Germinal Vesicle ◽  
Gonad Maturation ◽  
Anabas Testudineus ◽  
Germinal Vesicle Breakdown ◽  
Hormone Synthesis ◽  
Climbing Perch ◽  
Egg Diameter ◽  
Stimulation Of

<p>Gonadotropin can quicken the process of 17α-hidroxyprogesterone hormone synthesis becoming 17α, 20β-di hidroxyprogesterone as the maturation inducing steroids (MIS) and quicken the process of egg nucleus integration to germinal vesicle breakdown (GVBD) position. This research aimed to know the efficacy of gonadotropin hormone in the form of ovaprim on gonad maturation of climbing perch (<em>Anabas testudineus</em> Bloch). Stimulation of GtH with a dose of 0.5 ml/kg of fish body weight could improve the fish gonado somato index (GSI) to 2.72 %, improve the the final egg diameter to  0.70 mm (71.50 %), and shorten ovulation time which down to 4.30 hours.</p> <p>Key words: Gonadotropin hormone, gonad maturation, <em>Anabas testudineus</em></p> <p><em> </em></p> <p>ABSTRAK</p> <p>Hormon gonadotropin dapat mempercepat proses sintesa hormon 17α-hidroksiprogesteron menjadi 17α, 20β-dihidroksiprogesteron yang berfungsi sebagai steroid yang merangsang pematangan gonad dan mempercepat proses integrasi inti sel telur menuju posisi <em>germinal vesicle breakdowan</em> (GVBD). Penelitian ini bertujuan untuk mengetahui efektivitas hormon gonadotropin yang terdapat dalam ovaprim terhadap pematangan gonad ikan betook <em>Anabas testudineus</em> Bloch. Pemberian hormon GtH dalam bentuk ovaprim dengan dosis 0,5 ml/kg bobot ikan dapat memperbaiki perkembangan gonad yang ditunjukkan dengan peningkatan nilai gonado somatik indeks (GSI) sebanyak 2,72%, peningkatan diameter telur menjadi 0,77 mm (71,50%) dan mempercepat waktu ovulasi menjadi 4,3 jam.</p> <p>Kata-kata kunci: Hormon gonadotropin, pematangan gonad, ikan betok</p>

scholarly journals Comparison between Single and Double Injection of Pituitary Gland (PG) on the Breeding Performance of Climbing Perch, Anabas Testudineus (Bloch)

1970 ◽  
Vol 14 ◽  
pp. 57-60
Author(s):  
Shahid Uddin Mahmood
Keyword(s):  
Pituitary Gland ◽  
Key Words ◽  
Single Injection ◽  
Breeding Performance ◽  
Female Fish ◽  
Anabas Testudineus ◽  
Double Injection ◽  
Climbing Perch ◽  
Randomized Design ◽  
Completely Randomized Design

An investigation was carried out to study the effects of single and double injection of given dose of pituitary gland (PG) on the breeding performance viz., ovulation response, fertilization, and hatching of climbing perch, Anabas testudineus was tested. Pituitary gland dose of 1.2 mg/100g was used in this experiment. Twenty female fish was arranged into two treatments: T1 and T2, each with 10 replications. In T1, the entire dose was administered in a single injection and in T2 the same dose was given in two equal injections three hr apart. The experiment was laid in completely randomized design (CRD). It was found that administration of PG in split doses (double injection) had no additional advantages (ovulation 100%, fertilization 81.56% and hatching 72.68%) over the single injection (ovulation 100%, fertilization 83.03% and hatching 75.48%) treatment. Similarly, the ovulation time of the fish did not vary significantly in either single or double injection of PG (single injection 7.12 hr; double injection 7.20 hr). The results demonstrated that splitting of pituitary gland dose (double injection) has no additional advantages on breeding performance of A. testudineus. Therefore, single injection may be preferred over the second injection for breeding of this fish. Key words: Breeding, pituitary gland (PG), fertilization, hatching J. bio-sci. 14: 57-60, 2006

DOMESTIFIKASI IKAN BETOK Anabas testudineus MELALUI PEMIJAHAN DI WADAH BUDIDAYA (CLIMBING PERCH, Anabas testudineus DOMESTICATION THROUGH SPAWNING)

2016 ◽  
Vol 2 (4) ◽  
pp. 188
Author(s):  
Untung Bijaksana ◽  
Septiodrian R Balantek
Keyword(s):  
Rainy Season ◽  
Gonadal Development ◽  
Water Levels ◽  
Natural State ◽  
Anabas Testudineus ◽  
Climbing Perch ◽  
Latent Time ◽  
Egg Diameter ◽  
Fish Gonads ◽  
Spawning Success

<p>Penelitian ini dimaksudkan sebagai upaya domestikasi Ikan Betok melalui pemijahan dalam wadah budidaya. Penelitian ini dilakukan di Laboratorium Basah Program Studi Budidaya Perairan Universitas Lambung Mangkurat . Tiga poin utama dalam mendukung keberhasilan pemijahan ikan, yaitu : perkembangan gonad , ovulasi dan pemijahan . Tentu perkembangan panjat bertengger gonad ikan terjadi pada awal musim kemarau puncak musim dan di awal musim hujan atau saat musim hujan , pemijahan terjadi . Keadaan alami ini mencoba untuk diadopsi dalam budidaya kontainer , dengan fashting selama 1 , 2 dan 3 hari dengan tingkat air , 10 , 15 dan 20 cm . Hasilnya adalah waktu yang laten dari 11,7 jam sampai 39 jam , fekunditas antara 5310-7376 butir , diameter telur 0,5 hingga 0,8 mm , tingkat fertilisasi antara 88,2-93,2 persen dan tingkat menetas antara 87 , 0-91,6 persen.</p><p>The study is intended as an attempt domestication through climbing perch spawning in the container cultivation. The research was conducted at the Laboratory of Wetlands Program University Faculty of Fisheries Aquaculture Lambung Mangkurat. Three main points in support of the spawning success of fish, namely: gonadal development, ovulation and spawning. Naturally development climbing perch fish gonads occurred in the early dry season to peak season and at the beginning of the rainy season or during the rainy season, spawning occurred. This natural state is trying to be adopted in the cultivation of container, with fashting for 1, 2 and 3 days with water levels, 10, 15 and 20 cm. The result is a latent time of 11.7 hours to 39 hours, fecundity between 5310 to 7376 grains, egg diameter 0.5 to 0.8 mm, the degree of fertilization between 88.2 to 93.2 percent and the degree of hatching between 87, 0 to 91.6 percent.</p>

scholarly journals Efficacy of Some Antibiotics used for the Treatment of Diseased Koi (Anabas Testudineus) Fish

2014 ◽  
Vol 3 (1) ◽  
pp. 7-8
Author(s):  
Md Mamnur Rashid ◽  
Muhammad Forhad Ali ◽  
Muhammad Mamunur Rahman ◽  
Ashish Kumar Saha
Keyword(s):  
Body Weight ◽  
Aeromonas Hydrophila ◽  
Recommended Dose ◽  
Anabas Testudineus ◽  
Isolation Medium ◽  
Climbing Perch ◽  
Amoxicillin Trihydrate ◽  
Esculin Hydrolysis ◽  
Higher Doses

Naturally diseased climbing perch Anabas testudineus was confirmed to be caused by Aeromonas hydrophila bacteria by aeromonas isolation medium (AIM), 0/129 vibriostatic agent and esculin hydrolysis. Such naturally diseased koi fish weretreated with four antibiotics: captor (chlortetracycline hydrochloride BP 45%), renamox 15% -Vet (amoxicillin trihydrate BP), oxy-Dox-F (oxytetracycline 20% and + doxycycline 10%), renaquine 20% -Vet (flumequine) at lower, recommended and higher doses were performed to examine the efficacy of the drugs. Captor was given at doses of 0.8, 1.0 and 1.2 g/3 litres of water separately. Doses of renamox 15% -Vet were given at 0.8, 1.0 and 1.2 g/litre of water. Doses of oxy-Dox-F were 0.8, 1.0 and 1.5 g/Kg body weight and doses of renaquine were 10, 12, and 15 mg/Kg body weight. Among the four antibiotics, effect of captor and renaquine at recommended dose showed the best result where 100% fish were recovered. However, renamox and oxy-Dox-F showed best result at higher dose. DOI: http://dx.doi.org/10.3329/mh.v3i1.19770 Microbes and Health, June 2014. 3(1): 7-8

scholarly journals BIOLOGI REPRODUKSI IKAN BETOK (Anabas testudineus) DI PAPARAN BANJIRAN LUBUK LAMPAM,KABUPATEN OGAN KOMERING ILIR

2015 ◽  
Vol 6 (3) ◽  
pp. 137
Author(s):  
Eko Prianto ◽  
Mohammad Mukhlis Kamal ◽  
Ismudi Muchsin ◽  
Endi Setiadi Kartamihardja
Keyword(s):  
Sex Ratio ◽  
Anabas Testudineus ◽  
Commercial Fish ◽  
Male And Female ◽  
Climbing Perch ◽  
Male Sex ◽  
First Maturity ◽  
Egg Diameter ◽  
Floodplain Area ◽  
Somatic Index

Ikan betok (Anabas testudineus) adalah salah satu jenis ikan ekonomis penting yang dihasilkan dari perairan paparan banjiran. Penelitian yang bertujuan untuk mengkaji beberapa aspek biologi reproduksi ikan betok di paparan banjiran Lubuk Lampam, Kabupaten Ogan Komering Ilir dilakukan pada bulan Nopember 2012-Oktober 2013. Sampel ikan ditangkap setiap bulanmenggunakan alat tangkap jaring dan bengkirai.Analisis data meliputi sebaran frekuensi ukuran panjang, nisbah kelamin, tingkat kematangan gonad, indeks kematangan gonad, ukuran pertamakali matang gonad, potensi reproduksi dan pola reproduksi. Jumlah sampel ikan betok yang diperoleh sebanyak 540 ekor, terdiri dari 187 ekor ikan jantan dan 353 ekor ikan betina, dengan kisaran panjang ikan betina antara 27-224 mm dan ikan jantan antara 48-243 mm. Rasio kelamin ikan jantan dan betina adalah 0,53 : 1. Tingkat kematangan gonad (TKG) ikan jantan dan betina yang paling banyak ditemui adalah TKG I dengan frekuensi tertinggi pada ukuran 116-132 mm dan 114-129 mmmasing-masing berjumlah 34 dan 33 ekor. Perkembangan tingkat kematangan gonad ikan betok dipengaruhi oleh perubahan tinggimuka air secara musiman. Indek kematangan gonad ikan jantan pada TKGIV berkisar 1,3-15,0%dan ikan betina berkisar antara 1,2 17,1%. Ukuran pertama kali matang gonad ikan betina adalah pada panjang total 160 mm dan ikan jantan pada panjang total 177 mm. Fekunditas ikan betok berkisar antara 224–182.736 butir dengan diameter telur berkisar antara 0,465-1,026 mmdengan pola pemijahan secara sebagian. Climbing perch, Anabas testudineus is a dominant commercial fish inhabit floodplain area of Lubuk Lampam, Ogan Komering Ilir Regency. A study aimed to investigate some aspects of the reproductive biology of climbing perch has been conducted at floodplain of Lubuk Lampam, Ogan Komering Ilir regency from November 2012 to October 2013. Fish sampling was conducted every month using nets and bamboo trap. The data analysis includes lenght frequency distribution, sex ratio, the gonado maturity, gonado somatic index, the size at first maturity, fecundity and reproductive patterns. Climbing perch sample amounted of 540 specimen compose of 187 males and 353 females, with the lenght frequency between 27-224 mm (female) and 48-243 mm (male). Sex ratio of the male and female of the climbing perch was 0,53 : 1. The gonado maturity of male and female are mostly at the first level with the highest frequency between 116-132 mm and 114-129 mm, equivalent to 34 and 33 specimen, respectively. The development of gonado maturity of climbing perch was influenced by seasonally of water level fluctuation. Gonado somatic index of maturity of male and female range 1.3-15.0% and 1.2 -17.1%, respectively. The size at the first maturity of the female was 160 mm lenght and of the male was 177 mm lenght. The fecundity ranges 224 to182,736 eggs with the egg diameter ranges 0.465-1.026 mm and the climbing perch was classified into partially spawner.

scholarly journals Comparison between cage and pond production of Thai Climbing Perch (Anabas testudineus) and Tilapia (Oreochromis niloticus) under three management systems

1970 ◽  
Vol 8 (2) ◽  
pp. 313-322 ◽  
Author(s):  
MN Mondal ◽  
J Shahin ◽  
MA Wahab ◽  
M Asaduzzaman ◽  
Y Yang
Keyword(s):  
Body Weight ◽  
Mixed Culture ◽  
Oreochromis Niloticus ◽  
Management Systems ◽  
Anabas Testudineus ◽  
Pond Aquaculture ◽  
Open Pond ◽  
Climbing Perch ◽  
Pond Culture ◽  
The Mean

An experiment was conducted to compare production and economic performance of Thai Climbing Perch (Anabas testudineus) and Tilapia (Oreochromis niloticus) under three management systems. The nursed juveniles of Thai Climbing Perch (6.22 ± 0.15g) and Tilapia (22.52 ± 0.73g) were stocked at 50 Thai Climbing Perch per 1m3 cage and 50 Tilapia per 80m2 open pond (Caged Perch); 50 Tilapia per 1m3 cage and 50 Thai Climbing Perch per 80 m2 open pond (Caged Tilapia); and both 50 Thai Climbing Perch and 50 Tilapia per 80m2 pond (Mixed culture) as three treatments with three replicates for each. Pelleted feed (35% crude protein) was given twice daily (8.0 h and 16.0 h) at a rate of 10% body weight of Thai Climbing Perch for first month and 5% body weight of Thai Climbing Perch for rest of the culture period (90 days) to cages for the integrated cage-pond culture and to open ponds for the mixed culture. Among the measured water quality parameters transparency (cm), alkalinity (mg l-1), nitrite-nitrogen (mg l-1), and chlorophyll-a (μg l-1) were significantly different among the treatments. A total of 43 genera of phytoplankton and 16 genera of zooplankton were identified from the pond water. The mean abundance of total macro-benthic organisms was not significantly different (P>0.05). The mean survival rate of Thai Climbing Perch was high, ranging from 86.67% to 98.67%. Gross yield of Thai Climbing Perch was the highest in the Caged Perch. Survival of Tilapia was also high, ranging from 94.00% to 96.67%. The combined FCRs were 0.75, 0.77 and 0.85 in the Caged Perch, Mixed culture and Caged Tilapia systems, respectively. Economic analysis revealed that a significantly higher (P<0.05) cost-benefit ratio was obtained in the Caged Perch treatment. Therefore, it is concluded that the integrated cage-pond culture system with the high-valued Thai Climbing Perch in cages and low-valued Tilapia in open ponds may be a better option for rural pond aquaculture considering the production and economic benefit. Keyword: Cage culture; Integrated aquaculture; Climbing Perch; Tilapia DOI: 10.3329/jbau.v8i2.7943 J. Bangladesh Agril. Univ. 8(2): 313-322, 2010  

scholarly journals Effects of GnRH and Anti-Dopamine on Gonad Maturation of Osteochilus melanopleurus (Bleeker, 1852)

2021 ◽  
Vol 934 (1) ◽  
pp. 012014
Author(s):  
N Asiah ◽  
N Aryani ◽  
N Nuraini ◽  
I Lukistyowaty ◽  
Y Harjoyudanto
Keyword(s):  
Body Weight ◽  
Research Method ◽  
Economic Value ◽  
Gonad Maturation ◽  
Gonad Maturity ◽  
River Waters ◽  
Randomized Design ◽  
Injection Dose ◽  
Completely Randomized Design ◽  
Egg Diameter

Abstract The Osteochilus melanopleurus (Bleeker, 1852) is one of the endogenous fish in the Siak River waters of Riau province which has high economic value. However, the fulfillment of needs still depends on the catch in nature. Efforts to overcome these problems are the availability of mature gonads. Broadly to accelerate the maturation of gonads through hormone induction until the fish are ready to be spawned to produce fish fry continuously. The purpose of this study was to analyze the effect of gonadotropin and anti-dopamine hormone induction with the trademark “Oodev” on the final gonad maturity (TKG) of the prospective broodstock of O. melanopleurus. The research was conducted from April to August 2021 in the fish rearing column and the experimental pond, Faculty of Fisheries and Marine Science. The research method was a Completely Randomized Design with 4 treatment levels and 3 replications, while the treatments were P0 (without Oodev injection), P1 (Oodev dose 0.5 mL/kg), P2 (0.7 mL/kg), and P3 (0,9 mL/kg). Measured responses: percentage of broodstock that reached TKG and egg diameter. The results of the research that have been carried out, the measurement of the diameter of the eggs produced showed that the use of the Oodev hormone at a dose of 0.7 mL/kg body weight of O.melanopleurus given every week for twelve weeks was able to stimulate the development of the gonads of O.melanopleurus. Ovaprim injection of 0.7 mL/kg body weight produced 89,566 eggs with a latency of 4 hours. oodev injection dose of 0.7 ml/kg BW or P2 treatment has succeeded in achieving a TKG of 82%.

scholarly journals Stimulation of gonad maturation in mullet fish Mugil dussumieri using MT, E2, hCG, and Ovaprim hormone

2019 ◽  
Vol 18 (1) ◽  
pp. 9-22
Author(s):  
Tatak Dwi Cahyono ◽  
Muhammad Zairin Junior ◽  
Odang Carman
Keyword(s):  
Body Length ◽  
Egg Size ◽  
Gonadosomatic Index ◽  
Physiological Solution ◽  
Gonad Maturation ◽  
Gonad Maturity ◽  
And Control ◽  
Egg Diameter ◽  
Estradiol 17Β ◽  
Stimulation Of

ABSTRACT The present study is a preliminary research for producing mullet fry to aquaculture. The research aimed to evaluate stimulation of gonad maturation in mullet (Mugil dussumieri) using hormones. The method used for research was completely randomized design consisting of three treatments and each individual replication was repeated three times. Two experiments were conducted separately with different treatments. First experiment used 9-14.7 cm body length of fish treated with different hormone injections i.e. 4 mg/kg 17α-methyltestosterone (MT), 0.07 mg/kg estradiol-17β (E2), and 0.5 ml/kg 0.9% physiological solution as control. Second experiment used 10-31 cm body length fish treated with 750 IU/kg chorionic gonadotropin (hCG), 0.5 ml/kg Ovaprim, and 0.9% physiological solution as control. Gonadosomatic index (GSI) value of the first experiment on day 60 showed that GSI of E2 treatment (1.31±0.94%) was higher than both MT treatment (1.00±0.51%) and control (0.54±0.20%). On the other hand, the second experiment on day 60 showed that GSI of hCG treatment (7.18±0.59%) was higher than both Ovaprim treatment (3.29±2.66%) and control (6.72±0.32%). Egg diameter frequency distribution for control in the first experiment on day 30 showed that egg size ranged from 9−144 µm. Egg diameter for E2 and MT treatments on day 60 showed that egg size ranged from 9−243 µm and were higher than control. In the second experiment, egg diameter on day 30 for control showed that egg size ranged from 9-144 µm, hCG treatment showed egg size ranged from 9−441 µm, while Ovaprim egg size ranged from 9-111 µm. Blood glucose, blood cholesterol, testosterone and estradiol hormone level in the first and second experiment showed no significant difference. The results showed that estradiol-17β and 17α-methyltestosterone induction in 9-14.7 cm body length mullet increase gonad maturity to stage II while hCG induction in 10-31 cm body length mullet increase gonad maturity to stage III. Keywords : estradiol-17β, hCG, 17α-methyltestosterone, Mugil dussumieri, Ovaprim ABSTRAK Penelitian merupakan rintisan untuk menghasilkan benih ikan belanak dalam wadah budidaya. Penelitian ini bertujuan untuk mengevaluasi pematangan gonad ikan belanak Mugil dussumieri menggunakan hormon. Penelitian dilakukan menggunakan rancangan acak lengkap yang terdiri dari tiga perlakuan dan tiga kali ulangan individu. Terdapat dua percobaan dengan perlakuan berbeda dan dilakukan secara terpisah. Percobaan pertama menggunakan ikan berukuran 9−14.7 cm dengan hormon 17α-metiltestosteron (MT) 4 mg/kg, estradiol-17β (E2) 0.07 mg/kg dan kontrol larutan fisiologis 0.9% 0.5 ml/kg. Percobaan kedua menggunakan ikan berukuran 10−31 cm dengan human chorionic gonadotropin (hCG) 750 IU/kg, Ovaprim 0.5 ml/kg dan kontrol larutan fisiologis 0.9 % 0.5 ml/kg. Nilai gonadosomatic index (GSI) percobaan pertama pada hari ke-60 menunjukkan bahwa pemberian estradiol-17β (1.31±0.94%) lebih tinggi dibandingkan 17α-metiltestosteron (1.00±0.51%) dan kontrol (0.54±0.20%). Hasil percobaan kedua pada hari ke-60 nilai GSI menunjukkan bahwa pemberian hCG (7.18±0.59%) lebih tinggi dibandingkan Ovaprim (3.29±2.66%) dan kontrol (6.72±0.32%). Sebaran frekuensi diameter telur pada percobaan pertama untuk kontrol hari ke-30 menunjukkan kisaran 9−144 µm. Sebaran frekuensi diameter telur untuk estradiol-17β dan 17α-metiltestosteron hari ke-60 menunjukkan kisaran 9−243 µm lebih banyak dibandingkan kontrol. Sebaran frekuensi diameter telur pada percobaan kedua untuk kontrol hari ke-30 menunjukkan kisaran 9−144 µm, hCG 9−441 µm sedangkan Ovaprim hanya 9−111 µm. Kadar glukosa darah, kolesterol darah, hormon testosteron dan estradiol pada percobaan pertama dan percobaan kedua menunjukkan tidak terdapat perbedaan yang nyata. Hasil penelitian menunjukkan bahwa induksi hormon estradiol-17β dan 17α-metiltestosteron pada ikan berukuran 9−14.7 cm dapat meningkatkan kematangan gonad mencapai TKG II sedangkan induksi hormon hCG pada ikan berukuran 10−31 cm dapat meningkatkan kematangan gonad mencapai TKG III. Kata kunci : estradiol-17β, hCG, 17α-metiltestosteron, Mugil dussumieri, Ovaprim

scholarly journals Ontogeny of the specificity of gonadotropin receptors and gene expression in carp

2019 ◽  
Vol 8 (11) ◽  
pp. 1433-1446 ◽  
Author(s):  
Lian Hollander-Cohen ◽  
Benjamin Böhm ◽  
Krist Hausken ◽  
Berta Levavi-Sivan
Keyword(s):  
Cyprinus Carpio ◽  
Gonad Development ◽  
Germinal Vesicle ◽  
Evolutionary Model ◽  
Receptor Activation ◽  
Ecological Niches ◽  
Germinal Vesicle Breakdown ◽  
Gonadotropin Receptors ◽  
Hormone Synthesis ◽  
Gonadotropin Receptor

The pituitary gonadotropins, luteinizing hormone (LH) and follicle-stimulating hormone (FSH), are the principle endocrine drivers of reproductive processes in the gonads of jawed vertebrates. Canonically, FSH recruits and maintains selected ovarian follicles for maturation and LH induces the stages of germinal vesicle breakdown and ovulation. In mammals, LH and FSH specifically activate cognate G-protein-coupled receptors that affect the proteins involved in steroidogenesis, protein hormone synthesis, and gametogenesis. This dual-gonadotropin model also exists in some fish species, but not in all. In fact, due to their diverse number of species, extended number of ecological niches, and remarkably flexible reproductive strategies, fish are appropriate as models to understand the co-evolution of gonadotropins and their receptors. In this study, we cloned and characterized the expression profile over the final stages of ovarian maturation of carp (Cyprinus carpio) LHCGR and FSHR. Expression of both gonadotropin receptors increased in the later stage of early vitellogenesis, suggesting that both LH and FSH play a role in the development of mature follicles. We additionally tested the activation of cLHCGR and cFSHR using homologous and heterologous recombinant gonadotropins in order to gain insight into an evolutionary model of permissive gonadotropin receptor function. These data suggest that carp (Cyprinus carpio) gonad development and maturation depends on a specific gonadotropin profile that does not reflect the temporally distinct dual-gonadotropin model observed in salmonids or mammals, and that permissive gonadotropin receptor activation is a specific feature of Ostariophysi, not all teleosts.

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