scholarly journals Efficacy of Some Antibiotics used for the Treatment of Diseased Koi (Anabas Testudineus) Fish

2014 ◽  
Vol 3 (1) ◽  
pp. 7-8
Author(s):  
Md Mamnur Rashid ◽  
Muhammad Forhad Ali ◽  
Muhammad Mamunur Rahman ◽  
Ashish Kumar Saha
Keyword(s):  
Body Weight ◽  
Aeromonas Hydrophila ◽  
Recommended Dose ◽  
Anabas Testudineus ◽  
Isolation Medium ◽  
Climbing Perch ◽  
Amoxicillin Trihydrate ◽  
Esculin Hydrolysis ◽  
Higher Doses

Naturally diseased climbing perch Anabas testudineus was confirmed to be caused by Aeromonas hydrophila bacteria by aeromonas isolation medium (AIM), 0/129 vibriostatic agent and esculin hydrolysis. Such naturally diseased koi fish weretreated with four antibiotics: captor (chlortetracycline hydrochloride BP 45%), renamox 15% -Vet (amoxicillin trihydrate BP), oxy-Dox-F (oxytetracycline 20% and + doxycycline 10%), renaquine 20% -Vet (flumequine) at lower, recommended and higher doses were performed to examine the efficacy of the drugs. Captor was given at doses of 0.8, 1.0 and 1.2 g/3 litres of water separately. Doses of renamox 15% -Vet were given at 0.8, 1.0 and 1.2 g/litre of water. Doses of oxy-Dox-F were 0.8, 1.0 and 1.5 g/Kg body weight and doses of renaquine were 10, 12, and 15 mg/Kg body weight. Among the four antibiotics, effect of captor and renaquine at recommended dose showed the best result where 100% fish were recovered. However, renamox and oxy-Dox-F showed best result at higher dose. DOI: http://dx.doi.org/10.3329/mh.v3i1.19770 Microbes and Health, June 2014. 3(1): 7-8

scholarly journals Experimental Infection of Indigenous Climbing Perch Anabas testudineus with Aeromonas hydrophila Bacteria

2013 ◽  
Vol 22 (1-2) ◽  
pp. 105-114 ◽  
Author(s):  
M Farhad Hossain ◽  
M Mamnur Rahman ◽  
MA Sayed
Keyword(s):  
Body Weight ◽  
Water Temperature ◽  
Aeromonas Hydrophila ◽  
Bacterial Load ◽  
Infected Fish ◽  
Pathogenicity Test ◽  
Anabas Testudineus ◽  
Climbing Perch ◽  
Average Body ◽  
Average Body Weight

The present study was conducted to know the pathogenicity and LD50 of Aeromonas hydrophila isolated from diseased climbing perch Anabas testudineus against apparently healthy homologous fish and the distribution of the bacteria in the organs of the experimentally infected fish. A total of 10 fish of average body weight of 18 g were used. For pathogenicity test, two different doses viz. 9.2 × 107 and 9.2 × 106 CFU/fish were injected intramuscularly. Pathogenicity of A. hydrophila was confirmed at water temperature of 28.53oC by the mortality of 40% to 100% of all tested fish within 4 to 9 days. The highest bacterial load was found to be 9.4 × 108 CFU/g in the intestine and the lowest bacterial load was found to be 2.8 × 103 CFU/g in the kidney of the tested fish. Four different serial concentrations, vide 9.2 × 107, 9.2 × 106, 9.2 × 105 and 9.2 × 104 CFU/fish of the bacteria were injected in each of four different groups of 10 fish. The calculated LD50 value at 27.3oC water temperature was 2 × 107 CFU/fish of 18 g of average body weight. In all the cases of intramuscular injection, external pathology was found. Reddish anal region and fin bases were observed. Injected A. hydrophila was re-isolated from liver, kidney and intestine of the challenged fish. It was understood that the isolate was a high virulent pathogen for A. testudineus.DOI: http://dx.doi.org/10.3329/pa.v22i1-2.16472 Progress. Agric. 22(1 & 2): 105-114, 2011

scholarly journals Comparison between cage and pond production of Thai Climbing Perch (Anabas testudineus) and Tilapia (Oreochromis niloticus) under three management systems

1970 ◽  
Vol 8 (2) ◽  
pp. 313-322 ◽  
Author(s):  
MN Mondal ◽  
J Shahin ◽  
MA Wahab ◽  
M Asaduzzaman ◽  
Y Yang
Keyword(s):  
Body Weight ◽  
Mixed Culture ◽  
Oreochromis Niloticus ◽  
Management Systems ◽  
Anabas Testudineus ◽  
Pond Aquaculture ◽  
Open Pond ◽  
Climbing Perch ◽  
Pond Culture ◽  
The Mean

An experiment was conducted to compare production and economic performance of Thai Climbing Perch (Anabas testudineus) and Tilapia (Oreochromis niloticus) under three management systems. The nursed juveniles of Thai Climbing Perch (6.22 ± 0.15g) and Tilapia (22.52 ± 0.73g) were stocked at 50 Thai Climbing Perch per 1m3 cage and 50 Tilapia per 80m2 open pond (Caged Perch); 50 Tilapia per 1m3 cage and 50 Thai Climbing Perch per 80 m2 open pond (Caged Tilapia); and both 50 Thai Climbing Perch and 50 Tilapia per 80m2 pond (Mixed culture) as three treatments with three replicates for each. Pelleted feed (35% crude protein) was given twice daily (8.0 h and 16.0 h) at a rate of 10% body weight of Thai Climbing Perch for first month and 5% body weight of Thai Climbing Perch for rest of the culture period (90 days) to cages for the integrated cage-pond culture and to open ponds for the mixed culture. Among the measured water quality parameters transparency (cm), alkalinity (mg l-1), nitrite-nitrogen (mg l-1), and chlorophyll-a (μg l-1) were significantly different among the treatments. A total of 43 genera of phytoplankton and 16 genera of zooplankton were identified from the pond water. The mean abundance of total macro-benthic organisms was not significantly different (P>0.05). The mean survival rate of Thai Climbing Perch was high, ranging from 86.67% to 98.67%. Gross yield of Thai Climbing Perch was the highest in the Caged Perch. Survival of Tilapia was also high, ranging from 94.00% to 96.67%. The combined FCRs were 0.75, 0.77 and 0.85 in the Caged Perch, Mixed culture and Caged Tilapia systems, respectively. Economic analysis revealed that a significantly higher (P<0.05) cost-benefit ratio was obtained in the Caged Perch treatment. Therefore, it is concluded that the integrated cage-pond culture system with the high-valued Thai Climbing Perch in cages and low-valued Tilapia in open ponds may be a better option for rural pond aquaculture considering the production and economic benefit. Keyword: Cage culture; Integrated aquaculture; Climbing Perch; Tilapia DOI: 10.3329/jbau.v8i2.7943 J. Bangladesh Agril. Univ. 8(2): 313-322, 2010  

scholarly journals Isolation and identification of Aeromonas hydrophila from silver carp and its culture environment from Mymensingh region

2014 ◽  
Vol 11 (2) ◽  
pp. 373-376 ◽  
Author(s):  
M Mamnur Rashid ◽  
MS Hossain ◽  
MF Ali
Keyword(s):  
Aeromonas Hydrophila ◽  
Pathogenic Bacteria ◽  
Bacterial Flora ◽  
Bacterial Load ◽  
Silver Carp ◽  
Hypophthalmichthys Molitrix ◽  
Isolation And Identification ◽  
Isolation Medium ◽  
Liver And Kidney ◽  
Esculin Hydrolysis

The pathogenic bacteria, Aeromonas hydrophila from naturally infected silver carp, Hypophthalmichthys molitrix were isolated and identified from a fish farm in Mymensingh. Fish showed motile Aeromonas septicemia (MAS) like reddish head and anal region, pale body colour and external haemorrhages. Intestine, liver, kidney and environmental samples such as sediment, feed and water were inoculated onto the Aeromonas isolation medium (AIM) and TSA plates. The AIM colonies were undergone specific characterization for Aeromonas hydrophila. Quantitative studies of bacterial flora of fish on TSA plates showed that the total bacterial load in intestine, liver and kidney of the sampled fish, sediment, feed and water of the environment were 1.00 × 105 to 1.50 × 105 CFU/g, 2.7 × 102 to 4.46 × 104 CFU/g, 1.00 × 103 to 2.17 × 103 CFU/g, 1.90 × 104 CFU/g, 4.10 × 103 CFU/g and 2.50 × 103 CFU/ml, respectively. After isolation, A. hydrophila was finally identified by their specific morphological, physiological and biochemical characteristics. They were gram negative, rod shaped, motile bacteria that showed positive reaction for oxidase and catalase, fermented glucose and were resistant to vibriostatic agent 0/129. They showed positive result in esculin hydrolysis test. DOI: http://dx.doi.org/10.3329/jbau.v11i2.19943 J. Bangladesh Agril. Univ. 11(2): 373-376, 2013

scholarly journals Study of gonadotropin (GtH) stimulating hormone on gonad maturation of climbing perch Anabas testudineus Bloch

2010 ◽  
Vol 9 (1) ◽  
pp. 61
Author(s):  
. Suriansyah ◽  
M. Zairin Junior ◽  
Agus Oman Sudrajat
Keyword(s):  
Body Weight ◽  
Key Words ◽  
Germinal Vesicle ◽  
Gonad Maturation ◽  
Anabas Testudineus ◽  
Germinal Vesicle Breakdown ◽  
Hormone Synthesis ◽  
Climbing Perch ◽  
Egg Diameter ◽  
Stimulation Of

<p>Gonadotropin can quicken the process of 17α-hidroxyprogesterone hormone synthesis becoming 17α, 20β-di hidroxyprogesterone as the maturation inducing steroids (MIS) and quicken the process of egg nucleus integration to germinal vesicle breakdown (GVBD) position. This research aimed to know the efficacy of gonadotropin hormone in the form of ovaprim on gonad maturation of climbing perch (<em>Anabas testudineus</em> Bloch). Stimulation of GtH with a dose of 0.5 ml/kg of fish body weight could improve the fish gonado somato index (GSI) to 2.72 %, improve the the final egg diameter to  0.70 mm (71.50 %), and shorten ovulation time which down to 4.30 hours.</p> <p>Key words: Gonadotropin hormone, gonad maturation, <em>Anabas testudineus</em></p> <p><em> </em></p> <p>ABSTRAK</p> <p>Hormon gonadotropin dapat mempercepat proses sintesa hormon 17α-hidroksiprogesteron menjadi 17α, 20β-dihidroksiprogesteron yang berfungsi sebagai steroid yang merangsang pematangan gonad dan mempercepat proses integrasi inti sel telur menuju posisi <em>germinal vesicle breakdowan</em> (GVBD). Penelitian ini bertujuan untuk mengetahui efektivitas hormon gonadotropin yang terdapat dalam ovaprim terhadap pematangan gonad ikan betook <em>Anabas testudineus</em> Bloch. Pemberian hormon GtH dalam bentuk ovaprim dengan dosis 0,5 ml/kg bobot ikan dapat memperbaiki perkembangan gonad yang ditunjukkan dengan peningkatan nilai gonado somatik indeks (GSI) sebanyak 2,72%, peningkatan diameter telur menjadi 0,77 mm (71,50%) dan mempercepat waktu ovulasi menjadi 4,3 jam.</p> <p>Kata-kata kunci: Hormon gonadotropin, pematangan gonad, ikan betok</p>

scholarly journals Isolation and characterization of two virulent Aeromonads associated with haemorrhagic septicaemia and tail-rot disease in farmed climbing perch Anabas testudineus

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Abhishek Mazumder ◽  
Hrishikesh Choudhury ◽  
Abhinit Dey ◽  
Dandadhar Sarma
Keyword(s):  
Natural Infection ◽  
Winter Season ◽  
Pcr Amplification ◽  
Body Parts ◽  
Anabas Testudineus ◽  
Climbing Perch ◽  
Isolation And Characterization ◽  
Biochemical Characterisation ◽  
Rot Disease

AbstractDiseased Anabas testudineus exhibiting signs of tail-rot and ulcerations on body were collected from a fish farm in Assam, India during the winter season (November 2018 to January 2019). Swabs from the infected body parts were streaked on sterilized nutrient agar. Two dominant bacterial colonies were obtained, which were then isolated and labelled as AM-31 and AM-05. Standard biochemical characterisation and 16S rRNA and rpoB gene sequencing identified AM-31 isolate as Aeromonas hydrophila and AM-05 as Aeromonas jandaei. Symptoms similar to that of natural infection were observed on re-infecting both bacteria to disease-free A. testudineus, which confirmed their virulence. LC50 was determined at 1.3 × 104 (A. hydrophila) and 2.5 × 104 (A. jandaei) CFU per fish in intraperitoneal injection. Further, PCR amplification of specific genes responsible for virulence (aerolysin and enterotoxin) confirmed pathogenicity of both bacteria. Histopathology of kidney and liver in the experimentally-infected fishes revealed haemorrhage, tubular degeneration and vacuolation. Antibiotic profiles were also assessed for both bacteria. To the best of our knowledge, the present work is a first report on the mortality of farmed climbing perch naturally-infected by A. hydrophila as well as A. jandaei, with no records of pathogenicity of the latter in this fish.

scholarly journals Pengaruh C/N Rasio Berbeda untuk Pembentukan Bioflok pada Media Pemeliharaan terhadap Pertumbuhan dan Kelangsungan Hidup Benih Ikan Betok (Anabas testudineus)

2019 ◽  
Vol 7 (1) ◽  
Author(s):  
M. Indra Wahyu Pratama ◽  
Dade Jubaedah ◽  
Mohamad Amin
Keyword(s):  
Survival Rate ◽  
Culture Media ◽  
Farming System ◽  
Anabas Testudineus ◽  
Growth And Survival ◽  
Climbing Perch ◽  
Randomized Design ◽  
Absolute Growth ◽  
Biofloc Technology ◽  
Absolute Length

Pratama et al, 2018. The Effect of Different C/N Ratio in for Biofloc of Formation Culture Media to the Growth and Survival Rate of Juvenile Climbing Perch (Anabas testudineus). JLSO 7(1): Climbing perch fish resources still have a problem due to this fish is still obtained only through capture and also the growth of climbing perch fish is slow. The solutions to increase the growth of the climbing perch fish is by applying biofloc technology for climbing perch fish culture. The purpose of this research was to know the effect of different C/N ratio on the growth of climbing perch fish (Anabas testudineus) juvenille with biofloc farming system. This research used a completely randomized design consist of four treatments and three replications. The treatments were without molase (P0), molase with C/N ratio 15 (P1), C/N 20 (P2) and C/N ratio 25 (P3). The results showed that C / N ratio 20 was the best treatment with absolute length of growth of 1.33 cm, absolute growth of weight 1.92 g, survival rate of 99.17% and feed efficiency 138.06%.

scholarly journals Prevalence and antibiotic susceptibility of Aeromonas hydrophila isolated from freshwater fishes

2016 ◽  
Vol 4 (3) ◽  
pp. 411 ◽  
Author(s):  
Halima Sarder ◽  
Tahsin Khan ◽  
Mihir Lal Saha ◽  
Nusrat Jahan Punom ◽  
Shankar Chandra Mandal ◽  
...  
Keyword(s):  
Aeromonas Hydrophila ◽  
Antibiotic Susceptibility ◽  
Reference Strain ◽  
Bacterial Count ◽  
Freshwater Fishes ◽  
Total Bacterial Count ◽  
Bacterial Isolates ◽  
Biochemical Tests ◽  
Anabas Testudineus ◽  
Significant Difference

Aeromonas hydrophila is an opportunistic microorganism. It is a secondary biological agent that contributes to the occurrence of fish diseases and its deterioration. This research was undertaken to determine the prevalence of A. hydrophila in some freshwater fishes collected from three different fish markets of Dhaka City and to test their antibiotic susceptibility. Total bacterial count and total aeromonas on different aeromonas selective media were enumerated using serial dilution technique. Bacterial isolates were characterized to identify A. hydrophila using biochemical tests and with comparison to reference strain (ATCC 7966). The lowest Aeromonas count was detected to be 2.83±0.40×102 cfu/g in Anabas testudineus and the highest was 1.03±0.153×103 cfu/g in Oreochromis mossambicus. On market basis highest aeromonas count was found in Anando Bazar (8.10±1.09×102 cfu/g) and lowest in Hatirpool Bazar (5.63±0.90×102 cfu/g) with no significant difference. Maximum susceptibility to amikacin and gentamicin was observed whereas all of the isolates were found resistant to a commonly used antibiotic amoxycillin. The obtained results point that antimicrobial susceptibility was more or less similar regardless of the origin of the samples collected. All the fishes investigated in this study contained A. hydrophila in their different organs.

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