scholarly journals Effects of GnRH and Anti-Dopamine on Gonad Maturation of Osteochilus melanopleurus (Bleeker, 1852)

2021 ◽  
Vol 934 (1) ◽  
pp. 012014
Author(s):  
N Asiah ◽  
N Aryani ◽  
N Nuraini ◽  
I Lukistyowaty ◽  
Y Harjoyudanto
Keyword(s):  
Body Weight ◽  
Research Method ◽  
Economic Value ◽  
Gonad Maturation ◽  
Gonad Maturity ◽  
River Waters ◽  
Randomized Design ◽  
Injection Dose ◽  
Completely Randomized Design ◽  
Egg Diameter

Abstract The Osteochilus melanopleurus (Bleeker, 1852) is one of the endogenous fish in the Siak River waters of Riau province which has high economic value. However, the fulfillment of needs still depends on the catch in nature. Efforts to overcome these problems are the availability of mature gonads. Broadly to accelerate the maturation of gonads through hormone induction until the fish are ready to be spawned to produce fish fry continuously. The purpose of this study was to analyze the effect of gonadotropin and anti-dopamine hormone induction with the trademark “Oodev” on the final gonad maturity (TKG) of the prospective broodstock of O. melanopleurus. The research was conducted from April to August 2021 in the fish rearing column and the experimental pond, Faculty of Fisheries and Marine Science. The research method was a Completely Randomized Design with 4 treatment levels and 3 replications, while the treatments were P0 (without Oodev injection), P1 (Oodev dose 0.5 mL/kg), P2 (0.7 mL/kg), and P3 (0,9 mL/kg). Measured responses: percentage of broodstock that reached TKG and egg diameter. The results of the research that have been carried out, the measurement of the diameter of the eggs produced showed that the use of the Oodev hormone at a dose of 0.7 mL/kg body weight of O.melanopleurus given every week for twelve weeks was able to stimulate the development of the gonads of O.melanopleurus. Ovaprim injection of 0.7 mL/kg body weight produced 89,566 eggs with a latency of 4 hours. oodev injection dose of 0.7 ml/kg BW or P2 treatment has succeeded in achieving a TKG of 82%.

scholarly journals THE STIMULATION OF GONAD MATURITY OF ASIAN REDTAIL CATFISH Hemibagrus nemurus (Valenciennes, 1840) THROUGH INDUCTION OF OOCYTE DEVELOPER (Oodev) HORMONE

2019 ◽  
Vol 8 (1) ◽  
pp. 965
Author(s):  
Devika Kharisma Putri ◽  
Tarsim Tarsim ◽  
Deny Sapto Chondro Utomo ◽  
Indra Gumay Yudha
Keyword(s):  
Research Method ◽  
Spawning Season ◽  
Gonad Maturity ◽  
Gonadal Maturity ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Egg Diameter ◽  
Somatic Index ◽  
Stimulation Of

Oodev hormone application has been applied to stimulate gonadal maturity so that fish fry could be available outside the spawning season. This research aimed to study the effect of Oodev hormone on the gonadal maturity of asian redtail catfish Hemibagrus nemurus (Valenciennes, 1840). This research method used a completely randomized design (CRD) with 4 doses treatments, i.e 0, 0,25, 0,50, 1,00 ml/kg. A total of 8 fish were used as replications for each treatment. The parameters measured were the gonado somatic index (GSI) and egg diameter. The results showed that the effect of Oodev hormone on GSI and egg diameter was significantly different (P<0.05). A higher Oodev hormone dose caused an increase of GSI and egg diameter size. The recommended Oodev dose for application is 1 ml/kg.

scholarly journals The Maturity of Female Gonads of G2 Transgenic Mutiara Catfish (Clarias sp.)

2021 ◽  
pp. 9-19
Author(s):  
Ayuniar Puteri ◽  
Ibnu Dwi Buwono ◽  
Roffi Grandiosa Herman ◽  
. Iskandar
Keyword(s):  
Reproductive Performance ◽  
Clarias Gariepinus ◽  
Transgenic Fish ◽  
Egg Weight ◽  
Gonad Maturity ◽  
Gonadal Maturity ◽  
Randomized Design ◽  
Gonad Growth ◽  
Completely Randomized Design ◽  
Egg Diameter

The G2 trangsenic Mutiara catfish (Clarias sp.) (MTG) is a Mutiara catfish that is inserted with the CgGH gene (Clarias gariepinus Growth Hormone) through the transgenesis. The effect of transgenesis stimulates gonad growth of G2 transgenic Mutiara catfish (Clarias sp.) faster than non-transgenic fish. Study aimed to analyze the maturity of the gonads and the spawning ability of female G2 transgenic Mutiara catfish (Clarias sp.) to obtain superior broodstock candidates. Experimental method with completely randomized design (pair of parents used as treatment and repeated four times) for spawning was used for this study. Three pairs of parent G2 were crossed semi-artificially as treatment A (female 1 MTG G2 crossed with male 1 MTG G2), B (female 2 MTG G2 crossed with male 2 MTG G2) and C (female 3 MTG G2 crossed with male 3 MTG G2). The results showed that the performance of female G2 transgenic Mutiara catfish (Clarias sp.) (treatment A, B and C) was higher given non-transgenic with an average relative fecundity of 82,438 eggs / kg of broodstock, an average egg diameter of 1.76 mm and an average egg weight 1.75 mg. These indications suggest that GH transgenesis increases gonadal maturity. The gonad maturity profile reached the stage of complete maturity (full ripe) compared to Sangkuriang catfish (Clarias gariepinus) (immature gonads). Therefore it is necessary to compare the reproductive performance of G2 transgenic Mutiara catfish (Clarias sp.) with non-transgenic fish (Sangkuriang) as candidates for superior broodstock of catfish.

scholarly journals Hormonal induction maturation of silver pompano Trachinotus blochii

2020 ◽  
Vol 19 (1) ◽  
pp. 61-73
Author(s):  
Wiwin Kusuma Atmaja Putra ◽  
Tengku Said Raza’i
Keyword(s):  
Body Weight ◽  
Gonadosomatic Index ◽  
Hormone Treatment ◽  
Hepatosomatic Index ◽  
Gonad Maturation ◽  
Maturity Level ◽  
Randomized Design ◽  
Absolute Growth ◽  
Completely Randomized Design ◽  
Trachinotus Blochii

ABSTRACT   This study was aimed to acquire the most suitable hormone treatment to induce gonad maturation of silver pompano Trachinotus blochii. This study used a completely randomized design with four treatments and three replications, i.e NaCl 90% (control) (0.5 mL/kg), hCG (20 IU/kg fish body weight), PMSG (20 IU/kg fish body weight), and PG600 (20 IU/kg fish body weight). Parameters measured were gonadosomatic index (GSI), hepatosomatic index (HSI), gonad maturity level (TKG), gonad histology, and absolute growth. The best result was PG600 treatment with 0.12% gonadosomatic index, 2.29% hepatosomatic index, TKG IV, and 85 g absolute growth for four weeks. This study concludes that the most suitable hormone to induce gonad maturation of silver pompano Trachinotus blochii is PG600 with 20 IU/kg body weight, which effectively affects GSI, HSI, and absolute growth of silver pompano.   Keywords: hCG, maturation, PG600, PMSG, silver pompano.   ABSTRAK   Penelitian ini bertujuan untuk mendapatkan hormon yang terbaik untuk menginduksi maturasi gonadikan bawal bintang. Hormon yang digunakan pada penelitian ini adalahkontrol NaCl 90% (dosis 0,5 mL/kg),  hCG(dosis 20 IU/kg bobot tubuh ikan), PMSG (dosis 20 IU/kg bobot tubuh ikan), dan PG600 (dosis 20 IU/kg bobot tubuh ikan). Parameter yang diamati adalah indeks gonadosomatik (GSI), indeks hepatosomatik (HSI), tingkat kematangan gonad (TKG), histologi gonad, dan pertumbuhan bobot mutlak. Hasil terbaik adalah perlakuan PG600 dengan gonadosomatik indeks (0,12%), hepatosomatik indeks (2,29%), TKG IV,dan pertumbuhan mutlak (85 g) selama empat minggu. Kesimpulan penelitian ini adalah perlakuan hormone yang berpotensi menginduksi maturasi gonad ikan bawal bintang adalah hormon PG600 dengan dosis 20 IU/kg bobot tubuh dikarenakan adanya pengaruh nyata tehadap parameter GSI, HSI dan pertumbuhan mutlak ikan bawal bintang.   Kata kunci: ikan bawal bintang, hCG, PMSG, PG600, maturasi.  

scholarly journals Substitusi rumput lapang dengan hay African Star Grass (Cynodon plectostachyus) terhadap produktivitas kelinci lokal jantan

2021 ◽  
Vol 5 (1) ◽  
pp. 31-36
Author(s):  
Syifa Nurjannah ◽  
Ikhsan Kholiq ◽  
Tedi Akhdiat ◽  
Nilawati Widjaya
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Research Method ◽  
Body Weight Gain ◽  
Feed Conversion ◽  
Randomized Design ◽  
Affect Body Weight ◽  
Spss Software ◽  
Completely Randomized Design ◽  
Range Test

The purpose of this research was to determine field grass substitution by hay African Star Grass (Cynodon plectostachyus) on the productivity of local male rabbits with an age of 3 months old. The research was conducted in October-November 2020 in Central Pojok Village, Cikahuripan, Lembang, West Bandung Regency. The research method used a completely randomized design with five treatments which were P0: 80% field grass + 20% concentrate; P1: 60% field grass + 20% hay African Star Grass + 20% concentrate; P2: 40% field grass + 40% hay African Star Grass + 20% concentrate; P3: 20% field grass + 60% hay African Star Grass + 20% concentrate; P4: 80% African Star Grass hay + 20% concentrate, and each treatment was repeated five times so that the number of rabbits used were 25 heads. Data obtained were analyzed using SPSS software and Duncan's multiple range test. The results showed that the substitution of field grass by Afrikan Star Grass affected ration consumption (P<0.05) with an average of 150.79-185.78 grams/head/day, but did not affect body weight gain and feed conversion (P>0.05) with an average of 14.64-19.78 grams/head/day and 7.94-11.60, respectively. This study concluded that the provision of hay African Star Grass at the level of 20% showed more optimal results.

scholarly journals The Gonad Maturity of Female Osteochillus vittatus in the presence of Ascorbic Acid

2017 ◽  
Vol 9 (2) ◽  
Author(s):  
Nuning Setyaningrum ◽  
Sugiharto Sugiharto ◽  
Hexa Apriliana Hidayah
Keyword(s):  
Ascorbic Acid ◽  
Survival Rate ◽  
Food Supplement ◽  
Gonad Maturation ◽  
Oocyte Diameter ◽  
Fish Production ◽  
Maturity Level ◽  
Gonad Maturity ◽  
Randomized Design ◽  
Completely Randomized Design

<p>The fish’s reproduction status is affected by both fed compositions and vitamins intake lead to determine improvement of eggs quality as well as fish production. The presence of Ascorbic Acid (AA) in the cultivation ponds, might accelerate female’s gonad maturation and so rematuration. The research  aimed to determine: (1) Level gonad maturity of supplementation Ascorbic Acid; (2) Oocyts diameter; (3) Larvae survival rate. The research used experimental methods.The method was a completely randomized design (CRD) of 4 treatments and 4 replications.  Treatments were supplementation of AA at different dosages of 0, 600, 1200 and 1800 mg/kg fish-fed ration.The gonad maturity level was analysed descriptively and oocyte diameter and larvae survival rate data were analysed  by  ANOVA. The result showed that supplementation of AA at a dosage of 1200 mg / kg fish-fed ration accelerated the process of gonad maturity, development of oocyt diameter and larvae survival rate as well as the viability of <em>O. vittatus</em> larvae at 90 rearing days.<em>  </em>In this case, level IV gonad maturity was reached at 90 days which characterized by completed vitelogenesis process and oocyte diameter of 1.1 mm.  Thus, this study useful for aquaculture science by provide information on utilization of ascobic acid as food supplement in fish culture and also for fish farmer who wish to accelerate <em>O. vittatus</em> reproduction.</p>

Pengaruh Pemberian Moringa oleifera Terhadap Kuantitas dan Kualitas Semen Sapi Peranakan Ongole

2020 ◽  
Vol 7 (2) ◽  
pp. 119
Author(s):  
Lukman Affandhy ◽  
Muchamad Luthfi ◽  
Dian Ratnawati ◽  
Frediansyah Firdaus
Keyword(s):  
Data Analysis ◽  
Analysis Of Variance ◽  
Research Method ◽  
Moringa Oleifera ◽  
The Third ◽  
Randomized Design ◽  
Leaf Powder ◽  
Completely Randomized Design ◽  
Second Period

ABSTRAK  Penelitian bertujuan untuk mengetahui pengaruh pemberian bubuk daun Moringa oleifera (MO) terhadap kuantitas dan kualitas semen sapi peranakan ongole (PO). Metode penelitian menggunakan percobaan lapang dengan rancangan acak lengkap dengan tiga perlakuan pemberian MO sebesar 0 kg/ekor/hari (P1); 0,05 kg/ekor/hari (P2) dan 0,1 kg/ekor/hari (P3). Analsis data menggunakan one way analysis of variance. Masing-masing perlakuan terdiri atas empat ekor dengan tiga periode pengamatan sebagai ulangan, yaitu periode pertama dan ketiga tanpa MO, dan periode kedua diberikan MO pada P2 dan P3. Hasil penelitian menunjukan bahwa volume dan pH semen perlakuan P1, P2 dan P3 tidak berbeda semua periode pengamatan. Motilitas massa spermatozoa P2 dan P3 periode kedua dan ketiga menunjukan nilai +++ berbeda nyata (P<0,05) dibandingkan dengan P1 (++); motilitas individu  perlakuan P1 menunjukkan nilai terendah 60,56±2,94 % (P<0,05) dibandingkan P2 dan P3 pada periode kedua dan ketiga (75,50±2,29 dan 72,50±2,34%). Konsentrasi spermatozoa P2 dan P3 periode kedua dan ketiga menunjukkan rata-rata  >1.350 juta/ml berbeda nyata (P<0,05) daripada P1 (876±152juta/ml), sedangkan total spermatozoa motil menunjukkan nilai tertinggi 5.647±829 juta/ml pada P3 periode kedua.Viabilitas spermatozoa P2 dan P3 menunjukkan nilai >88-89 % beda nyata (P<0,05) daripada P1 (<84 %) pada periode kedua dan ketiga, sedangkan nilai abnormalitas spermatozoa yang terbaik adalah P2 sebesar 4,30% pada periode kedua dan P3 sebesar 5,33% pada periode ketiga. Disimpulkan pemberian bubuk daun MO dengan dosis 0,1 kg/ekor/hari dapat memperbaiki kuantitas dan kualitas semen (viabilitas dan total spermatozoa motil) sapi pejantan PO.Kata kunci:  kuantitas dan kualitas semen, Moringa oleifera, sapi pejantan ABSTRACTThe research aimed to determine the effect of Moringa oleifera (MO) leaf powder on the quantity and quality of semen of ongole crossbreed. The research method used was a field experiment with a completely randomized design with three treatments giving MO of 0 kg/head/day (P1); 0.05 kg/head/day (P2) and 0.1 kg/head/day (P3). Each treatment consisted of four heads with three periods of observation as replications, namely the first and third periods without MO, and the second period was given MO on P2 and P3. Data analysis used a one-way analysis of variance. The results showed that the volume and pH of semen treated P1, P2, and P3 didn’t different in all observation periods. The second and third period spermatozoa mass motility of P2 and P3 showed that the value of +++ was significantly different (P<0.05) compared to P1 (++); Individual motility of P1 treatment showed the lowest value 60,56±2,94 % (P<0.05) compared to P2 and P3 in the second and third periods (75,50±2,29 and 72,50±2,34%). The second and third periods of P2 and P3 spermatozoa concentrations showed an average of >1,350 million/cc significantly different (P<0.05) than P1 (876±152 million/cc), while the total motile spermatozoa showed the highest value of 5,647±829 million/cc in the second period P3. The spermatozoa viability of P2 and P3 showed values >88-89% significantly different (P<0.05) than P1 (<84%) in the second and third periods, while the best spermatozoa abnormalities were P2 at 4.30% in the second period and P3 at 5.33% in the third period. It was concluded that the giving of MO leaf powder at a dose of 0,1 kg/head/day could improve the quantity and quality of semen (viability and total motile spermatozoa) of Ongole Crossbreed bulls. Keywords: bulls, Moringa oleifera, quantity and quality of semen

scholarly journals PERFORMANCE OF QUAILS (Coturnix-coturnix japonica) GIVEN DRINK CONTAINING MORINGA LEAF SOLUTION IN THEIR GROWING PERIOD

2019 ◽  
Vol 4 (2) ◽  
pp. 75
Author(s):  
Fatkur Rohman ◽  
Ristika Handarini ◽  
Hanafi Nur
Keyword(s):  
Drinking Water ◽  
Body Weight ◽  
Egg Production ◽  
Body Weight Gain ◽  
Coturnix Japonica ◽  
Feed Conversion ◽  
Coturnix Coturnix ◽  
Growing Period ◽  
Randomized Design ◽  
Completely Randomized Design

Moringa leaves contain phytochemicals which can be used to improve the performance of quails in their growing period.  This study was aimed at assessing the effects of the inclusion of moringa leaf solution in drinking water on the performance of quails.  The study was conducted at Badrupuyuh Farm, Sukajaya Village RT 01/02, Ciomas, Bogor Regency from October to December 2017.  One-hundred quail birds aged 7 days with average initial body weight of 17.87±0.46 g were used.  The birds were fed starter and grower commercial rations.  Moringa leaf solution was given through drinking water.  A completely randomized design with 4 treatments and 5 replicates was used.   Treatments consisted of drinking water containing no moringa leaf solution (R0), drinking water containing 5% moringa leaf solution (R1), drinking water containing 10% moringa leaf solution (R2), drinking water containing 15% moringa leaf solution (R3).  Data were subjected to an analysis of variance and a Duncan test.  Measurements were taken on feed intake, body weight gain (BWG), feed conversion, and mortality rate.  Results showed that there were significant effects (P<0.05) of treatments on BWG in week 2 and 4 and feed conversion in week 2.  No mortality of quails was found.  It was concluded that the inclusion of 10% moringa leaf solution in drinking water could increase BWG in week 2 and 4, improve feed conversion, and maintain the life of quails.  It was recommended that further studies on the effects of moringa leaf solution inclusion in drinking water on egg production in laying period.  Key words: performance, quail, moringa leaf solution

scholarly journals PENGARUH PENGGANTIAN JAGUNG DENGAN SORGUM Cv. KAWALI DALAM RANSUM PELLET TERHADAP PERFORMANS KELINCI LOKAL

ZOOTEC ◽  
2018 ◽  
Vol 39 (1) ◽  
pp. 42
Author(s):  
Agung R. P. Rumondor ◽  
B. Tulung ◽  
A. Rumambi ◽  
C.A Rahasia
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Analysis Of Variance ◽  
Body Weight Gain ◽  
Statistical Design ◽  
Feed Consumption ◽  
Feed Conversion ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
All Treatment

EFFECT OF REPLACEMENT OF CORN WITH SORGUM Cv. KAWALI IN PELLET RANSUM ON LOCAL RABBIT PERFORM. The purpose of this study was to determine the effect of substitution corn with sorghum in rabbit rations on feed consumption, body weight gain, and ration conversion. The duration of the study was 28 days using 20 local rabbits. The statistical design used in this study was a completely randomized design, with 4 (four) treatment rations and 5 (five) replications. The four experimental rations, namely R0: 45% corn + 0% sorghum, R1: 30% corn + 15% sorghum, R2 15% corn + 30% sorghum, R3 0% corn + 45% sorghum. Variables were measured, viz: feed consumption, body weight gain and ration conversion. The results showed that the average of feed consumption, body weight gain, and feed conversion, were: 28.27–30.59 g /head /day, 15.76–21.55 g / head /day, and 1.42-1.80 g /head /day, respectively. Based on the analysis of variance, the treatments ration had no significant effect (P> 0.05) on consumption, but had a very significant effect (P <0.01) on body weight gain and feed conversion. The LSD test showed that all treatment rations using sorghum (R1, R2, and R3) gave results of body weight gain which were very significantly different (P <0.01) and higher than R0. Whereas, on ration conversion, treatments R1, R2, and R3 give very different results (P <0.01) lower than R0. It is concluded that the substitution of corn with sorghum at the level of 45% in the ration gives the highest results for body weight gain and conversion of rations for rabbits. Keywords: corn, sorghum, ration, performance, local rabbit

scholarly journals Evaluasi Performa dan Income Over Feed & Chick Cost (IOFCC) Tiga Strain Ayam Broiler yang Beredar di Aceh

2020 ◽  
Vol 20 (2) ◽  
Author(s):  
Zulfan Zulfan ◽  
Zulfikar Zulfikar
Keyword(s):  
Body Weight ◽  
Broiler Chickens ◽  
Broiler Chicken ◽  
Body Weight Gain ◽  
Economic Value ◽  
Feed Conversion ◽  
Final Body Weight ◽  
Randomized Design ◽  
Different Strains ◽  
Better Than

ABSTRAK. Penelitian ini bertujuan untuk mengevaluasi performa tiga strain ayam broiler yang umumnya dipelihara di Aceh. Materi yang digunakan adalah 150 ekor anak ayam broiler (DOC) strain Cobb 500, CP 707, dan MB 202 masing-masing berjumlah 50 ekor. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) yang terdiri dari 3 perlakuan dan 5 ulangan. Setiap ulangan merupakan unit percobaan yang masing-masing terdiri dari 10 ekor anak ayam. Perlakuan adalah tiga strain ayam broiler: Cobb 500 (P1), CP 707 (P2), dan MB 202 (P3). Parameter yang diamati meliputi pertambahan berat badan, konsumsi ransum, konversi ransum (FCR), mortalitas, dan Income over Feed and Chick Cost (IOFCC). Hasil penelitian memperlihatkan performa broiler dipengaruhi oleh jenis strain. Strain MB 202 dan CP 707 memiliki bobot badan akhir (6 minggu) dan konsumsi ransum nyata (P0,05) lebih tinggi daripada Cobb 500. Angka konversi ransum tidak memperlihatkan perbedaan yang nyata antara ketiga strain pada semua umur panen. Nilai IOFCC tertinggi dipengaruhi oleh jenis strain dan lama pemeliharaan. Dari hasil penelitian dapat disimpulkan bahwa performa broiler dipengaruhi oleh kemampuan adaptasi strain terhadap iklim setempat (pesisir Aceh) pada periode berbeda-beda yang dapat memengaruhi tingkat keuntungannya. Strain Cobb 500 memiliki kemampuan adaptasi lebih baik selama periode awal, sedangkan CP 707 dan MB 202 selama periode akhir. (Evaluation of performances and income over feed chick cost (iofcc) of three broiler chicken strains commonly marketed in Aceh) ABSTRACT. The study aimed to evaluate the performances and Income over Feed and Chick Cost (IOFCC) of three broiler chicken strains commonly marketed in Aceh. As many as 150 chicks of three different broiler strains (50 birds each) were used in this study. The study was performed into Completely Randomized Design (CRD) consists of 3 treatments and 5 replications creating 15 experimental units containing 10 birds each. The treatments were 3 different strains of broiler chickens i.e. Cobb 500, CP 707, and MB 202. The birds were reared up to 6 weeks to expose their performances recorded as final body weight, body weight gain, feed intake, and feed conversion, as well as mortality. Economic value was evaluated by calculating IOFCC. Results of study showed that performances of broilers were significantly (P0,05) influenced by the strains. Strains MB 202 and CP 707 represented more superior than Cobb 500 for 5−6 weeks of raising periods. However, the later was not inferior during initial phase close to 4 weeks. There were no significant differences in FCR among all strains for different ages. The IOFCC related to the strains and their marketing ages. The highest IOFCC was obtained by Cobb 500, CP 707, and MB 202 as they were sold at the ages of 4, 5, and 6 weeks, respectively. In conclusion, the achievements of the performances in various broiler strains were affected by the site climate (Aceh) in different stages of their ages. Cobb 500 had proper adaptation during starter, while CP 707 and MB 202 appeared seem to be better than Cobb 500 during finisher period.

scholarly journals Promoting Color Brightness of Clown Loach (Chromobotia macracanthus Bleeker) by Mixing Red Spinach (Amaranthus tricolor L.) Powder with Feed Stuff

2020 ◽  
pp. 21-30
Author(s):  
Ayi Yustiati ◽  
Syakirah Imtinan Zurwana ◽  
Achmad Rizal ◽  
Yuli Andriani
Keyword(s):  
Research Method ◽  
Survival Rates ◽  
Optimal Dose ◽  
Primary Data ◽  
Test Results ◽  
Growth Data ◽  
Amaranthus Tricolor ◽  
Randomized Design ◽  
Powder Addition ◽  
Completely Randomized Design

The purpose of this research is to determine the optimal dose of the addition of red spinach powder to artificial feed on the brightness of clown loach. This research was conducted at Aquaculture Laboratory Building 4 Faculty of Fisheries and Marine Sciences of Universitas Padjadjaran from April to May 2020. This research method is experimental with a Completely Randomized Design consisting of four treatments and three replications. The red spinach powder addition treatment used 0%, 2%, 4%, and 6% of the feed amount. The parameters observed are color value as primary data by using Toca Color Finder, while the growth, survival rate, and water quality as support data. Color assessment results were analyzed using the Kruskal-Wallis test, if there were significant differences, Z test would be performed. Growth data and survival rates were analyzed using Analysis of Variance (ANOVA). Because the F test results were not significantly different, the Duncan test was not carried out. The results showed that the addition of  6% red spinach powder is the best treatment with an increased color brightness value of 5,63.

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