scholarly journals Factors influencing mycelial growth of Sclerotium rolfsii

2013 ◽  
Vol 1 ◽  
pp. 26-31 ◽  
Author(s):  
Imdramani Bhagat
Keyword(s):  
Mycelial Growth ◽  
Organic Nitrogen ◽  
Inorganic Nitrogen ◽  
Sclerotium Rolfsii ◽  
Nitrogen Sources ◽  
Maximum Growth ◽  
Optimum Conditions ◽  
Factors Influencing ◽  
The World ◽  
Better Than

Sclerotial blight of tea (Camellia sinensis L.) caused by Sclerotium rolfsii Sacc. is one of the destructive diseases in tea growing areas of the world. In the present investigation, an attempt was made to know the optimum conditions for the mycelial growth of S. rolfsii. Factors influencing mycelial growth of S. rolfsii were studied with special reference to their growth in different media, variable pH and variable sources of carbon (viz., 6 types) as well as organic (viz., 4 types) and inorganic (viz., 4 types) nitrogen sources. Maximum growth of pathogen occurred after 8 days of inoculation at pH 6. Dextrose was the most effective carbon source and yeast extract (organic source) was found most optimum for growth of S. rolfsii. Organic nitrogen sources were found to be better than inorganic nitrogen sources. DOI: http://dx.doi.org/10.3126/njbs.v1i0.7466 Nepalese Journal of Biosciences 1: 26-31 (2011)

Medium Optimization for the Production of Antioxidants from Aspergillus candidus

1999 ◽  
Vol 62 (6) ◽  
pp. 657-661 ◽  
Author(s):  
GOW-CHIN YEN ◽  
YUNG-CHI CHANG
Keyword(s):  
Antioxidant Activity ◽  
Organic Nitrogen ◽  
Strong Influence ◽  
Medium Optimization ◽  
Inorganic Nitrogen ◽  
Nitrogen Sources ◽  
Activity Inhibition ◽  
Aspergillus Candidus ◽  
Better Than ◽  
Ethyl Acetate Extracts

The objective of this study was to optimize the factors for the production of antioxidant from Aspergillus candidus CCRC 31543. Extracts of broth filtrate had higher antioxidant activity (inhibition of peroxidation [IP] >98%) when sucrose or lactose was used as a carbon source. Sucrose in the medium also resulted in a higher yield of extracts. Ethyl acetate extracts had the highest yield and antioxidant activity compared with the other two solvents. For the production of antioxidant, inorganic nitrogen sources were found to be more suitable than organic nitrogen sources, and ammonium sulfate was better than sodium nitrate. Yeast extract had a strong influence on the yield of antioxidant extracts. Both mycelium and broth filtrate of A. candidus CCRC 31543 showed similar antioxidant activity (IP = 95%), and they also had similar extraction yields.

Management of collar rot disease in chickpea by Trichoderma species

2020 ◽  
Vol 7 (03) ◽  
Author(s):  
PREM PANDEY ◽  
G. C. SAGAR ◽  
SUNDARMAN SHRESTHA2 ◽  
HIRAKAJI MANANDHAR ◽  
RITESH K. YADAV ◽  
...  
Keyword(s):  
Growth Inhibition ◽  
Mycelial Growth ◽  
Sclerotium Rolfsii ◽  
Maximum Growth ◽  
Pot Experiment ◽  
Trichoderma Spp ◽  
Trichoderma Species ◽  
Collar Rot ◽  
Rot Disease

Nine isolates of Trichoderma spp. were isolated from different agro- ecological regions of Nepal viz; Jumla, Palpa, Chitwan, Tarahara, Banke, Illam and Salyan and screened against Sclerotium rolfsii Sacc. Adreded soil borne phytopathogen causing collar rot of chickpea in chickpea; In-vitro efficacy of nine fungal antagonist (Trichoderma spp.) against Sclerotium rolfsii were screened. Pot experiment was done to find out the effective management of S. rolfsi through Tricoderma using different methods i.e. Seed treatment, soil drenching and soil application. All the tested isolates of Trichoderma spp. were found effective on mycelial growth inhibition and sclerotial parasitization of S. rolfsii. Trichoderma isolated from Palpa district showed maximum growth inhibition (%) of pathogen periodically after 48(93.78%), 72(96.00%), 96(97.96%) and 120(100.00%) hours of inoculation. Parasitized sclerotium showed minimum sclerotial germination on agar plates. Moreover, Trichoderma species isolated from Palpa districts showed second best percent mycelial growth inhibition periodically at 72(25.00%), 120(29.16%), 168(29.16%) and 216(29.16%).In pot experiment at 40 days after sowing, Seedling height was maximum in soil drenching with 30g per 100ml of water (22.27cm) and Mortality percentage of seedlings was least or highest disease control was observed in seed treated with 109cfu/ml (0.000%).

OPTIMIZATION OF BIOVANILLIN PRODUCTION OF LEMONGRASS LEAVES HYDROLYSATES THROUGH PHANEROCHAETE CHRYSOSPORIUM

2015 ◽  
Vol 77 (31) ◽  
Author(s):  
Huszalina Hussin ◽  
Madihah Md Salleh ◽  
Chong Chun Siong ◽  
Muhammad Abu Naser ◽  
Suraini Abd- Aziz ◽  
...  
Keyword(s):  
Ferulic Acid ◽  
Nitrogen Source ◽  
Phanerochaete Chrysosporium ◽  
Organic Nitrogen ◽  
Nitrogen Sources ◽  
Optimum Conditions ◽  
Molar Yield ◽  
Primary Screening ◽  
Organic Nitrogen Source ◽  
Inorganic And Organic Nitrogen

The recent study has demonstrated the effects of different nitrogen sources on vanillin production by Phanerochaete chrysosporium. Primary screening supported maximum biotransformation of ferulic acid (from lemongrass leaves hydrolysate) to vanillin by using ammonium chloride and yeast extract as inorganic and organic nitrogen source, respectively. With the 2-level factorial analysis, the optimum conditions of vanillin production from ferulic acid by P. chrysosporium was achieved at 0.192g/L with a molar yield of 24.5%.

Effects of Additional Stimulants on Phenol Degradation by a Halotolerant Rhodococcus sp. JDD1H

2012 ◽  
Vol 253-255 ◽  
pp. 914-920 ◽  
Author(s):  
Fang Yan Chen ◽  
Duo Duo Jiang ◽  
Yu Bin Tang
Keyword(s):  
Energy Source ◽  
Organic Nitrogen ◽  
High Salinity ◽  
Inorganic Nitrogen ◽  
Phenol Degradation ◽  
Nitrogen Sources ◽  
Tween 80 ◽  
Cane Sugar ◽  
Wide Range ◽  
Rhodococcus Sp

A halotolerant and highly effective phenol-degrading strain, named as JDD1H, was isolated from soil contaminated by petroleum and identified as Rhodococcus sp. Phenol degradation by strain JDD1H in the presence of high salinity, especially the effect of exotic stimulants such as carbon source, nitrogen source and surfactant on phenol degradation was investigated. The results show that strain JDD1H could utilize phenol as sole carbon and energy source to grow in the presence of 5% NaCl with 16 h adaptive phase. The degradation efficiency of 150 mg/L phenol was up to 99.5% within 40 h. Lactose, cane sugar, glucose and mannitol in wide range of 100~2000 mg/L enhanced phenol degradation significantly. Organic nitrogen sources, as peptone and yeast extract, and inorganic nitrogen sources, as NH4Cl and (NH4)2SO4, accelerated phenol degradation. Tween-80 with concentration of 50-1000 mg/L restrained phenol degradation and Tween-80 of 1500-2000 mg/L could promote degradation.

Interactions between nitrogen sources and xylose affecting growth, sporulation, and polyphenoloxidase activity in Bipolaris maydis race T

1981 ◽  
Vol 59 (11) ◽  
pp. 2102-2107 ◽  
Author(s):  
Robert C. Evans ◽  
Candace L. Black
Keyword(s):  
Amino Acids ◽  
Organic Nitrogen ◽  
Inorganic Nitrogen ◽  
Nitrogen Concentration ◽  
Relative Effectiveness ◽  
Nitrogen Sources ◽  
Dry Weight ◽  
The Other ◽  
Growth Media ◽  
Bipolaris Maydis

The effect of 23 organic and 3 inorganic nitrogen sources on growth, sporulation, and polyphenoloxidase activity was measured in Bipolaris maydis race T incubated with or without a xylose supplement. Mycelial dry weight tends to be highest on acidic and other polar amino acids and least on nonpolar ones; organic nitrogen sources generally result in greater dry weight than inorganic ones. Changes in nitrogen concentration and pH of media influence growth and sporulation, but only pH alters the relative effectiveness of the nitrogen sources on these processes. The addition of xylose to the growth media has little effect on growth. However, in the presence of lysine, serine, asparagine, glycine, γ-alanine, alanine, and α-aminobutyrate, xylose causes an increase in sporulation and a concomitant decrease in polyphenoloxidase activity. There is no consistent pattern resulting from xylose addition in the presence of the other nitrogen sources.

A NUTRITIONAL STUDY OF THREE STRAINS OF CLAVICEPS PURPUREA (FR). TUL.

1957 ◽  
Vol 3 (1) ◽  
pp. 1-12 ◽  
Author(s):  
W. A. Taber ◽  
L. C. Vining
Keyword(s):  
Organic Nitrogen ◽  
Inorganic Nitrogen ◽  
Methyl Cellulose ◽  
Nitrogen Sources ◽  
Tween 80 ◽  
Early Growth ◽  
Claviceps Purpurea ◽  
Nutritional Study ◽  
Ammonium Succinate ◽  
Exogenous Supply

Organic nitrogen sources were more readily utilized than inorganic nitrogen sources by three strains of Claviceps purpurea. Glucose, fructose, mannose, cellobiose, and sucrose were readily utilized, but not xylose, ribose, mannitol, maltose, starch, powdered cellulose, carboxymethyl cellulose, or methyl cellulose. Carbon dioxide was required for initiation of growth. An exogenous supply of biotin was required for growth in a medium containing glucose, ammonium succinate, and minerals. This requirement could be nearly replaced by equimolar amounts of biotin L-sulphoxide and biocytin. Desthiobiotin slightly replaced biotin but homobiotin, norbiotin, oleate, aspartate, pimelate, and Tween 80 were inactive. Oleate and aspartate stimulated early growth.

scholarly journals Effect of nitrogen supplementation on aerobic degradation of linear alkylbenzene sulfonate by consortia of bacteria

2012 ◽  
Vol 2 (1) ◽  
pp. 5
Author(s):  
Kehinde I. Temitope Eniola
Keyword(s):  
Organic Nitrogen ◽  
Linear Alkylbenzene Sulfonate ◽  
Micrococcus Luteus ◽  
Inorganic Nitrogen ◽  
Nitrogen Sources ◽  
Aerobic Degradation ◽  
Nitrogen Supplementation ◽  
Inorganic Nitrogen Source ◽  
Linear Alkylbenzene ◽  
Alkylbenzene Sulfonate

Untreated detergent bearing wastes discharged into the environment are sources of linear alkylbenzene sulfonate (LAS). Detergent wastes usually do not contain nitrogen or contain very low amounts. Biostimulation by introducing limiting nutrient element can be useful in biotreatment of such waste. The effect of inorganic and organic nitrogen supplements on aerobic degradation of LAS by LAS-utilizing bacteria was examined. Phosphate-buffered LAS mineral media were prepared and supplemented with different nitrogen sources: NPK fertilizer (inorganic) and urea fertilizer (organic). Individual and various consortia of pure cultures of <em>Alcaligenes odorans</em>, <em>Citrobacter diversus</em>, <em>Micrococcus luteus</em> and <em>Pseudomonas putida</em>, previously isolated from a detergent effluent polluted stream, were used. Biodegradation of LAS was monitored in terms of half-life (t&frac12;) of the surfactant. The rates of biodegradation by the consortia can be ranked as: 4-membered (t&frac12;=8-12 days) &gt;3-membered (t&frac12;=8-13 days) &gt;2-membered consortia (t&frac12;=10-15 days) &gt;individuals (t&frac12;=9-16 days). The inorganic nitrogen source enhanced utilization of the surfactant, while organic nitrogen supplementation generally slowed degradation of the surfactant. In undertaking biotreatment of detergent bearing effluent, inorganic nitrogen should be used as biostimulant.

scholarly journals Effects of nitrogen and carbon sources on the production of inulinase from strain Bacillus sp. SG113

2016 ◽  
Vol 3 (1) ◽  
pp. 69-74
Author(s):  
Simeon Gavrailov ◽  
Viara Ivanova
Keyword(s):  
Nitrogen Source ◽  
Organic Nitrogen ◽  
Inorganic Nitrogen ◽  
Carbon Sources ◽  
Casein Hydrolysate ◽  
Jerusalem Artichoke ◽  
Nitrogen Sources ◽  
Cellular Growth ◽  
Bacillus Sp ◽  
Carbon And Nitrogen

Abstract The effects of the carbon and nitrogen substrates on the growth of Bacillus sp. SG113 strain were studied. The use of organic nitrogen sources (peptone, beef extract, yeast extract, casein) leads to rapid cellular growth and the best results for the Bacillus strain were obtained with casein hydrolysate. From the inorganic nitrogen sources studied, the (NH4) 2SO4 proved to be the best nitrogen source. Casein hydrolysate and (NH4) 2SO4 stimulated the invertase synthesis. In the presence of Jerusalem artichoke, onion and garlic extracts as carbon sources the strain synthesized from 6 to 10 times more inulinase.

scholarly journals Nutritional characteristics and physiological studies on the growth and development of Phlebiopsis gigantea

2017 ◽  
Vol 8 ◽  
Author(s):  
Sushma Negi ◽  
Indu Bhushan Prasher ◽  
Rajeev Kumar
Keyword(s):  
Mycelial Growth ◽  
Inorganic Nitrogen ◽  
Nitrogen Compound ◽  
Nitrogen Sources ◽  
Physical Factors ◽  
Phlebiopsis Gigantea ◽  
Nutritional Characteristics ◽  
Physiological Studies ◽  
Peptone Medium

<p>Physiological studies pertaining to the effect of physical factors, carbon and nitrogen requirement of <em>Phlebiopsis gigantea</em> were conducted to know its behaviour <em>in vitro</em>. These studies have revealed interesting results regarding its growth and reproduction behaviour. The best medium for the optimum growth is Glucose-peptone medium. The optimum temperature (28°C) and pH (5.0) is required for the optimum mycelial production of <em>Phlebiopsis gigantea</em> after 12 days of incubation. The best carbon source for the growth of the fungus is D(+)Glucose least seen in Sucrose medium. The best inorganic nitrogen sources for the growth of the fungus is Sodium nitrate. The least mycelial growth of fungus is observed in ammonium acetate and ammonium nitrate. The best organic nitrogen compound for the mycelial growth of fungus is DL-threonine.</p>

Effect of the addition of nitrogen sources to cassava fiber and carbon-to-nitrogen ratios on Agaricus brasiliensis growth

2007 ◽  
Vol 53 (1) ◽  
pp. 139-143 ◽  
Author(s):  
T R.D Mantovani ◽  
G A Linde ◽  
N B Colauto
Keyword(s):  
Ammonium Sulfate ◽  
Agaricus Bisporus ◽  
Mycelial Growth ◽  
C:N Ratio ◽  
Fungal Growth ◽  
Nitrogen Sources ◽  
Agaricus Brasiliensis ◽  
Axenic Cultivation ◽  
Better Than

The same substratum formulation to grow Agaricus bisporus has been used to grow Agaricus brasiliensis since its culture started in Brazil. Despite being different species, many of the same rules have been used for composting or axenic cultivation when it comes to nitrogen content and source in the substrate. The aim of this study was to verify the mycelial growth of A. brasiliensis in different ammonium sulfate and (or) urea concentrations added to cassava fiber and different carbon-to-nitrogen (C:N) ratios to increase the efficiency of axenic cultivation. Two nitrogen sources (urea and (or) ammonium sulfate) added to cassava fiber were tested for the in vitro mycelial growth in different C:N ratios (ranging from 2.5:l to 50:l) in the dark at 28 °C. The radial mycelial growth was measured after 8 days of growth and recorded photographically at the end of the experiment. Nitrogen from urea enhanced fungal growth better than ammonium sulfate or any mixture of nitrogen. The best C:N ratios for fungal growth were from 10:l to 50:l; C:N ratios below 10:l inhibited fungal growth.Key words: Agaricus brasiliensis, Agaricus blazei, Agaricus subrufescens, nitrogen, C:N ratio.

Sign in / Sign up

Export Citation Format

Share Document