Chemical composition, antimicrobial and antifungal activity of Lippia Thymoide essential oil in oral pathogens

Aim: This study evaluated the chemical composition of Lippia thymoides (Lt) essential oil and its antimicrobial activity against fungal strains of Candida albicans (Ca) and Gram-negative bacteria Prevotella intermedia (Pi) and Fusobacterium nucleatum (Fn). Methods: Lt essential oil was obtained by hydrodistillation apparatus with a modified Clevenger extension. The chemical analysis was analyzed by gas phase chromatography and mass spectrometry on Shimadzu QP 2010 plus. Sample sensitivity evaluation was performed by ABHb-inoculum and culture plates were developed with triphenyltetrazolium chloride, also Fn and Pi samples analysis were in anaerobic environment and Ca sample analysis was performed in aerobic environment. The minimum inhibitory concentration (CIM) was determinated by microdilution in eppendorfs tubes. Results: The chemical analysis showed that Thymol (59,91%) is the main compound found in Lt essential oil, also other antifungal and antimicrobial agents were present γ-terpinene (8.16%), p-cymene (7.29%) and β-caryophyllene (4.49%), Thymol is a central ingredient of many medicinal plants and has a potent fungicidal, bactericidal and antioxidant activity, it has been previously shown to have anti-inflammatory activity against Periodontal Disease (PD) cause can reduces prostanoids, interleukins, leukotrienes levels in periodontium. CIM result Pi was 6.5 μg/mL, Fn was 1.5 μg/mL and Ca was 0.19 μg/mL. Conclusion: The antimicrobial activity of L. thymoides, through the compound Thymol, has been shown promising potential against gram-negative periodontopathogenic bacteria and fungi whose therapeutic arsenal is still very restricted.

Download Full-text

Screening of antimicrobial activity of essential oil and methanol extract of Satureja hortensis on foodborne bacteria and fungi

Czech Journal of Food Sciences ◽

10.17221/753-cjfs ◽

2008 ◽

Vol 25 (No. 2) ◽

pp. 81-89 ◽

Cited By ~ 43

Author(s):

A. Adiguzel ◽

H. Ozer ◽

H. Kilic ◽

B. Cetin

Keyword(s):

Antimicrobial Activity ◽

Chemical Composition ◽

Minimum Inhibitory Concentration ◽

Essential Oil ◽

Inhibitory Concentration ◽

Methanol Extract ◽

Antimicrobial Activities ◽

Satureja Hortensis ◽

Bacteria And Fungi

The present work reports the in vitro antimicrobial activities of the essential oil and methanol extract from Satureja hortensis as well as the content of its essential oil. The chemical composition of hydrodistilled essential oil of Satureja hortensis was analysed by means of GC-MS. Thirty constituents were identified. The main constituents of the oil were thymol (40.54%), γ-terpinene (18.56%), carvacrol (13.98%), and p-cymene (8.97). The essential oil of Satureja hortensis exhibited the activity against 25 bacteria, 8 fungi, and a yeast, C. albicans; exerting the Minimum Inhibitory Concentration values (MIC) ranging from 15.62 to 250 µl/ml. Similarly, methanol extract of the plant also showed antimicrobial activity.

Download Full-text

Chemical Composition And In-Vitro Antimicrobial Activity Of The Essential Oils Of Three Greek Achillea Species

Zeitschrift für Naturforschung C ◽

10.1515/znc-2002-3-415 ◽

2002 ◽

Vol 57 (3-4) ◽

pp. 287-290 ◽

Cited By ~ 72

Author(s):

Prokopios Magiatis ◽

Alexios-Leandros Skaltsounis ◽

Ioanna Chinou ◽

Serkos A. Haroutounian

Keyword(s):

Antimicrobial Activity ◽

Chemical Composition ◽

Essential Oil ◽

Essential Oils ◽

Pathogenic Fungi ◽

The Other ◽

Gram Negative ◽

In Vitro Antimicrobial Activity ◽

Ms Analysis

The chemical composition of the essential oils of Achillea holosericea, Achillea taygetea, Achillea fraasii was determined by GC/MS analysis. Among the ninety-five assayed constituents, camphor, borneol and 1,8-cineol were found to be the major components. The in-vitro antimicrobial activity of these essential oils was evaluated against six bacteria indicating that the first is totally inactive, while the other two possess moderate to strong activities mainly against the Gram negative strains. The essential oil of A. fraasii was also active against the tested pathogenic fungi

Download Full-text

Antimicrobial activity of some Iranian medicinal plants

Archives of Biological Sciences ◽

10.2298/abs1003633g ◽

2010 ◽

Vol 62 (3) ◽

pp. 633-641 ◽

Cited By ~ 36

Author(s):

Pirbalouti Ghasemi ◽

Parvin Jahanbazi ◽

Shekoofeh Enteshari ◽

Fatemeh Malekpoor ◽

Behzad Hamedi

Keyword(s):

Antimicrobial Activity ◽

Medicinal Plants ◽

Essential Oil ◽

Antimicrobial Agents ◽

Antimicrobial Activities ◽

Food Preservation ◽

Arnebia Euchroma ◽

Agar Disc ◽

Satureja Bachtiarica ◽

Bacteria And Fungi

The major aim of this study was to determine the antimicrobial activity of the extracts of eight plant species which are endemic in Iran. The antimicrobial activities of the extracts of eight Iranian traditional plants, including Hypericum scabrum, Myrtus communis, Pistachia atlantica, Arnebia euchroma, Salvia hydrangea, Satureja bachtiarica, Thymus daenensis and Kelussia odoratissima, were investigated against Escherichia coli O157:H7, Bacillus cereus, Listeria monocytogenes and Candida albicans by agar disc diffusion and serial dilution assays. Most of the extracts showed a relatively high antimicrobial activity against all the tested bacteria and fungi. Of the plants studied, the most active extracts were those obtained from the essential oils of M. communis and T. daenensis. The MIC values for active extract and essential oil ranged between 0.039 and 10 mg/ml. It can be said that the extract and essential oil of some medicinal plants could be used as natural antimicrobial agents in food preservation. .

Download Full-text

The In Vitro Antimicrobial Effects of Lavandula angustifolia Essential Oil in Combination with Conventional Antimicrobial Agents

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2016/2752739 ◽

2016 ◽

Vol 2016 ◽

pp. 1-9 ◽

Cited By ~ 15

Author(s):

Stephanie de Rapper ◽

Alvaro Viljoen ◽

Sandy van Vuuren

Keyword(s):

Antimicrobial Activity ◽

Essential Oil ◽

Antimicrobial Agents ◽

Inhibitory Concentration ◽

Antimicrobial Effect ◽

Antimicrobial Activities ◽

Lavandula Angustifolia ◽

Gram Negative ◽

Stock Solutions

The paper focuses on the in vitro antimicrobial activity of Lavandula angustifolia Mill. (lavender) essential oil in combination with four commercial antimicrobial agents. Stock solutions of chloramphenicol, ciprofloxacin, nystatin, and fusidic acid were tested in combination with L. angustifolia essential oil. The antimicrobial activities of the combinations were investigated against the Gram-positive bacterial strain Staphylococcus aureus (ATCC 6538) and Gram-negative Pseudomonas aeruginosa (ATCC 27858) and Candida albicans (ATCC 10231) was selected to represent the yeasts. The antimicrobial effect was performed using the minimum inhibitory concentration (MIC) microdilution assay. Isobolograms were constructed for varying ratios. The most prominent interaction was noted when L. angustifolia essential oil was combined with chloramphenicol and tested against the pathogen P. aeruginosa (ΣFIC of 0.29). Lavendula angustifolia essential oil was shown in most cases to interact synergistically with conventional antimicrobials when combined in ratios where higher volumes of L. angustifolia essential oil were incorporated into the combination.

Download Full-text

Chemical composition, antioxidative and antimicrobial activity of essential oil Ocimum sanctum L.

Hemijska industrija ◽

10.2298/hemind120615086b ◽

2013 ◽

Vol 67 (3) ◽

pp. 427-435 ◽

Cited By ~ 3

Author(s):

Damir Beatovic ◽

Slavica Jelacic ◽

Cedo Oparnica ◽

Dijana Krstic-Milosevic ◽

Jasmina Glamoclija ◽

...

Keyword(s):

Antimicrobial Activity ◽

Chemical Composition ◽

Essential Oil ◽

Antimicrobial Effect ◽

Raw Material ◽

Aspergillus Ochraceus ◽

Ocimum Sanctum ◽

Gram Negative ◽

Predominant Component ◽

Ocimum Tenuiflorum

Ocimum sanctum L. (Lamiaceae) sin. Ocimum tenuiflorum L. or Tulsi basil is a plant originating from tropical and subtropical areas of India. It is used in both the traditional and official medicine in India. Tulsi is a type of basil that is insufficiently explored and studied in Europe. The goal of this paper is to determine the chemical composition, antioxidative, and antimicrobial activity of the essential oil Ocimum sanctum L. grown in Serbia. The quantity of essential oil in 100 g of herb (v/w) is 0.68%, with 41 components identified in the tested essential oil. The most represented chemical group are sesquiturpene hydrocarbonates with 80.47%. Other groups were much less represented. Sesquiturpene hydrocarbonate ?-cariophyllene is a predominant component in the essential oil with 63.80%. The quantity of tested essential oil needed to achieve 50% of inhibition of DPPH radicals is 0.35 ?g/ml, and it has high potential to neutralize free radicals. The essential oil exhibited antibacterial activity to all tested strains of bacteria, both Gram-positive and Gram-negative. It affected all strains in an inhibitory way in the interval 0.34-41.50 ?l/ml, and in a bactericide way within the range 22.50-124.5 ?l/ml. The most sensitive strains of bacteria are Salmonella typhimurium and Escherichia coli, while Listeria monocytogenes and Enterococus faecalis showed greatest resistance. The essential oil exhibited antifugal activity on all tested fungi. It affected all tested fungi in an inhibitory way in the interval 4.42-8.83 ?l/ml, and in a microbicide way within the range 10.00-50.00 ?l/ml. The most sensitive fungi are: Aspergillus ochraceus, Penicillium ochrochloron and Penicilium funiculosum, while the most resistent one is Aspergillus niger. The tested basil essential oil Ocimum sanctum demonstrated significant antioxidative and antimicrobial effect and may be used as a raw material in food, pharmaceutical and chemical industries.

Download Full-text

Essential Oil Composition and Antimicrobial Activity of Ballota nigra L. ssp Foetida

Natural Product Communications ◽

10.1177/1934578x0900400429 ◽

2009 ◽

Vol 4 (4) ◽

pp. 1934578X0900400 ◽

Cited By ~ 1

Author(s):

Daniele Fraternale ◽

Anahi Bucchini ◽

Laura Giamperi ◽

Donata Ricci

Keyword(s):

Antimicrobial Activity ◽

Chemical Composition ◽

Essential Oil ◽

Essential Oil Composition ◽

Caryophyllene Oxide ◽

Oil Composition ◽

Gram Negative ◽

Gram Positive Bacteria ◽

Aerial Parts ◽

Germacrene D

The chemical composition of the essential oil of Ballota nigra L. ssp foetida obtained from the flowering aerial parts was analyzed by GC/MS. From the 37 identified constituents of the oil, β-caryophyllene (20.0%), germacrene D (18.0%) and caryophyllene oxide (15.0%) were the major components. The oil was active against both Gram-negative and Gram-positive bacteria as well as against three Candida species.

Download Full-text

Chemical Composition and Antioxidant, Antimicrobial, and Larvicidal Activities of the Essential Oils of Annona Salzmannii and A. pickelii (Annonaceae)

Natural Product Communications ◽

10.1177/1934578x1100600636 ◽

2011 ◽

Vol 6 (6) ◽

pp. 1934578X1100600 ◽

Cited By ~ 9

Author(s):

Emmanoel Vilaça Costa ◽

Lívia Macedo Dutra ◽

Hugo César Ramos de Jesus ◽

Paulo Cesar de Lima Nogueira ◽

Valéria Regina de Souza Moraes ◽

...

Keyword(s):

Antimicrobial Activity ◽

Chemical Composition ◽

Essential Oil ◽

Aedes Aegypti ◽

Essential Oils ◽

Antioxidant Capacity ◽

Bacteria And Fungi ◽

Main Components ◽

Larvicidal Activities ◽

Type Apparatus

The essential oils from the leaves of Annona salzmannii and A. pickelii (Annonaceae) growing in Sergipe, northeastern region of Brazil, were obtained by hydrodistillation using a Clevenger-type apparatus, and analyzed by GC/MS and GC/FID. Thirty-four compounds were identified in the essential oil of A. salzmannii and twenty-seven in that of A. pickelii; sesquiterpenes predominated in both essential oils. Bicyclogermacrene (20.3%), ( E)-caryophyllene (19.9%), δ-cadinene (15.3%), α-copaene (10.0%), and allo-aromadendrene (5.7%) were the main components of A. salzmannii, and bicyclogermacrene (45.4%), ( E)-caryophyllene (14.6%), and α-copaene (10.6%) of A. pickelii. The essential oils showed significant antioxidant capacity in the ORACFL and DPPH assays. The antimicrobial activity of these essential oils was also evaluated against bacteria and fungi, as well as the larvicidal activity against Aedes aegypti larvae.

Download Full-text

Chemical composition and antimicrobial activity of essential oils extracted from Amomum muricarpum Elmer from North Vietnam

Proceedings of universities Applied chemistry and biotechnology ◽

10.21285/2227-2925-2021-11-4-523-530 ◽

2022 ◽

Vol 11 (4) ◽

pp. 523-530

Author(s):

D. B. Thin ◽

V. Q. Thanh ◽

B. B. Thinh

Keyword(s):

Gas Chromatography ◽

Antimicrobial Activity ◽

Chemical Composition ◽

Essential Oil ◽

Essential Oils ◽

Antimicrobial Agents ◽

The Philippines ◽

Volatile Components ◽

Broth Microdilution Method ◽

North Vietnam

Abstract. Recent years have seen the development of bacterial resistance to currently available antibiotics, which necessitates a search for new antimicrobial agents. Amomum muricarpum Elmer is a widely used medicinal plant species in the genus Amomum (family Zingiberaceae) that is commonly found in Laos, the Philippines, China, and Vietnam. The present article describes the chemical composition and antimicrobial activity of essential oils extracted from the leaves and rhizomes of A. muricarpum from North Vietnam. The hydrodistilled essential oil was analyzed using gas chromatography and gas chromatography-mass spectrometry, with the broth microdilution method designed to evaluate its antimicrobial efficacy. The absolute yield of essential oils amounted to 0.11% and 0.13% (v/w) for leaves and rhizomes, respectively, on a dry weight basis. It was found that the leaves and rhizomes of A. muricarpum produce oils abounding in monoterpenes. Of the total identified volatile components in the leaf oil (97.18%), three main constituents include α-pinene (40.45%), linalool (12.34%), and β-pinene (10.31%). In the rhizome oil, the main constituents include α-pinene (48.10%), β-pinene (20.32%), and linalool (7.56%) of the total identified volatile components (98.08%). An antimicrobial activity test indicates that essential oils from the leaves and rhizome of A. muricarpum inhibit the growth of Staphylococcus aureus ATCC 25923, with a minimum inhibitory concentration (MIC) of 200 µg/ml. In addition, the rhizome essential oil also exhibits antimicrobial activity against Bacillus cereus ATCC 14579, with a MIC value of 200 µg/ml. The results indicate the potential of essential oils extracted from A. muricarpum as a source of antimicrobial agents.

Download Full-text

Synthesis and Evaluation of Some Coumarin Containing Potential Antimicrobial Agents

E-Journal of Chemistry ◽

10.1155/2012/953564 ◽

2012 ◽

Vol 9 (4) ◽

pp. 2493-2500

Author(s):

Sayali D. Kudale ◽

Meenakshi N. Deodhar

Keyword(s):

Antimicrobial Activity ◽

Antimicrobial Agents ◽

Gram Negative Bacteria ◽

Schiff’S Bases ◽

Gram Positive ◽

Gram Negative ◽

H Nmr ◽

Nmr Data ◽

Bacteria And Fungi

A series of the Schiff’s bases incorporating coumarin and chalcone moeities, 3-(4-(4-(substituted phenyl)prop-1-ene-3-one) phenylimino) methyl)-4-chloro-2h-chromen-2-one 4(a-g) were synthesized as potential antimicrobial agents. These compounds were characterized on the basis of their spectral (IR,1H NMR) data and evaluated for antimicrobial activity in vitro against gram positive and gram negative bacteria and fungi. Compound 4b was found to be most active with an MIC of 20 µg/mL against all the tested organisms.

Download Full-text

The chemical composition, antimicrobial and antioxidant activities of the essential oil of Salvia fruticosa growing wild in Libya

Archives of Biological Sciences ◽

10.2298/abs1301321g ◽

2013 ◽

Vol 65 (1) ◽

pp. 321-329 ◽

Cited By ~ 9

Author(s):

Abdulhamid Giweli ◽

Ana Dzamic ◽

Marina Sokovic ◽

M.S. Ristic ◽

P. Janackovic ◽

...

Keyword(s):

Antimicrobial Activity ◽

Antioxidant Activity ◽

Free Radical ◽

Chemical Composition ◽

Essential Oil ◽

Antioxidant Activities ◽

Radical Scavenging ◽

Free Radical Scavenging ◽

Gram Negative ◽

Salvia Fruticosa

The composition of essential oil isolated from Salvia fruticosa, wild growing in Libya, was analyzed by GC and GC-MS. Forty-five compounds could be identified. The essential oil contained 1,8-cineole (49.34%), camphor (7.53%), ?-pinene (7.38%), myrcene (7.38%), ?-pinene (5.15%), ?-caryophyllene (4.13%) and ?-terpineol (3.25). Antioxidant activity was analyzed using the DPP H free radical scavenging method and low antioxidant activity was found (IC50 = 15.53 mg/ml). The oil was also screened for its antimicrobial activity against eight bacteria (four Gram-negative and four Gram-positive) and eight fungi. The essential oil of S. fruticosa showed minimal inhibitory activity (MIC) at 0.125-1.5 mg/ml and bactericidal (MBC) at 0.5-2.0 mg/ml. In addition, it exhibited fungistatic (MIC) at 0.125-1.0 mg/ml and fungicidal effect (MFC) at 0.125-1.5 mg/ml.

Download Full-text