First Report on Molecular Identification of Caulerpa Green Algae from Mandangin Island Indonesia Using Partial 18SrRNA Genes

Caulerpa is one of the seaweed that grows naturally in Indonesian waters such as those in Mandangin Island. This study aimed to identify Caulerpa sp. based on molecular analysis using certain genetic markers. This research is expected to provide information on the identification of macroalgae from Indonesia waters, especially Mandangin Island, Madura with the use of molecular analysis based on 18SrRNA primers. The two green seaweed samples from the Caulerpa genus in this study were successfully analyzed using 18SrRNA primers. The BLAST results of samples 1and 2 are related to Caulerpa taxifolia 18SrRNA, but in the phylogenetic tree result, Sample 1 was more closely related to Caulerpa sertularioides f. longipes. 18SrRNA primers have been used for molecular identification of green seaweed from Mandangin for the first time and this shows that barcode markers can be used for molecular identification of seaweed, specifically Caulerpa in the waters of Mandangin Island, Indonesia.

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Hemiflagellochloris (Chlorophyceae, Chlorophyta), a new for Russian flora genus of green algae

Novosti sistematiki nizshikh rastenii ◽

10.31111/nsnr/2014.48.104 ◽

2014 ◽

Vol 48 ◽

pp. 104-113

Author(s):

A. D. Temraleeva ◽

E. V. Mincheva ◽

Yu. S. Bukin ◽

M. V. Eltsov ◽

V. A. Demkin ◽

...

Keyword(s):

Green Algae ◽

Molecular Analysis ◽

18S Rdna ◽

Phylogenetic Position ◽

Rdna Gene ◽

Algal Strain ◽

The North ◽

Volgograd Region ◽

First Time ◽

18S Rdna Gene

The genus Hemiflagellochloris Watanabe et al. was found in Russia for the first time. The algal strain was isolated from a buried solonetz in the zone of dry steppes (the north-end of Ergeni Hills, Volgograd Region). The morphology and ecology of this isolate are described. Its phylogenetic position within Chlorophyceae is determined by molecular analysis of 18S rDNA gene.

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Molecular detection of Rickettsia genus in chigger mites (Trombidiformes: Trombiculidae) collected on small mammals in southeastern brazilian

Revista Brasileira de Parasitologia Veterinária ◽

10.1590/s1984-29612019069 ◽

2019 ◽

Vol 28 (4) ◽

pp. 563-568

Author(s):

Fernando de Castro Jacinavicius ◽

Ricardo Bassini-Silva ◽

Sebastián Muñoz-Leal ◽

Cal Welbourn ◽

Ronald Ochoa ◽

...

Keyword(s):

Molecular Identification ◽

Small Mammals ◽

18S Rdna ◽

Molecular Detection ◽

Molecular Analyses ◽

First Report ◽

Rdna Sequences ◽

18S Rdna Sequences ◽

First Time

Abstract Chiggers are ectoparasites of vertebrates and may cause trombiculiasis or transmit pathogens to their hosts. Specimens collected from rodents and marsupials were morphologically identified as Herpetacarus hertigi, Eutrombicula tinami, Kymocta sp., Quadraseta brasiliensis, Quadraseta falconensis, Quadraseta flochi, Quadraseta mackenziei, Quadraseta pazca, Quadraseta trapezoides, Quadraseta sp., Serratacarus sp., and Trombewingia bakeri. These mites were submitted individually to molecular analyses for the detection of bacteria of the genus Coxiella, Hepatozoon and Rickettsia. Samples were positive to Rickettsia only. Obtained sequences for the gltA (350 pb) and ompA (488 pb) genes were identical to “Candidatus Rickettsia colombianensi”, a species previously detected in ticks. In addition, molecular identification of mites based on 18S rDNA sequences are provided for H. hertigi, Kymocta sp., Q. brasiliensis, Q. pazca, Q. trapezoides, Quadraseta sp., and T. bakeri for the first time. This is the first report of the detection of a Rickettsia sp. in chigger mites collected on rodents in Brazil.

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Molecular characterization and new reports of two green algae from Bangladesh

Bangladesh Journal of Plant Taxonomy ◽

10.3329/bjpt.v26i1.41915 ◽

2019 ◽

Vol 26 (1) ◽

pp. 39-45

Author(s):

Md. Almujaddade Alfasane ◽

Md. Miraj Kobad Chowdhury ◽

Maliha Mehnaz

Keyword(s):

Molecular Characterization ◽

Green Algae ◽

Molecular Identification ◽

18S Rdna ◽

Algal Species ◽

Rdna Sequences ◽

18S Rdna Sequences ◽

Nj Tree ◽

First Time ◽

Partial 18S

This communication portrays the molecular characterization and confirms the new reports of two fresh water green algae namely, Pithophora polymorpha Wittrock and Spirogyra maxima (Hassall) Wittrock from Bangladesh. The samples of these algal species were cultured and partial 18S rDNA was sequenced and analysed for their molecular identification. It was found that the primers reported here could sufficiently identify these algae as P. polymorpha and S. maxima. Furthermore, the Neighbour joining (NJ) tree generated from 18s rDNA sequences suggested that Spirogyra maxima of Bangladesh is distantly related to the cluster of S. juergensii and S. platensis. Pithophora polymorpha along with P. roettleri, P. sano and Pithophora sp. seems to form a strongly supported monophyletic group. The alga AP1 clusters with Pithophora and the alga AS1 clusters with Spirogyra. This study is the first-time report of molecular identification of Bangladeshi algae and a landmark towards the future exploration of thealgal biodiversity of Bangladesh.

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Molecular identification of five species of family Chaetomiaceae (Sordariomycetes, Ascomycota) from Iraqi soil

Biodiversitas Journal of Biological Diversity ◽

10.13057/biodiv/d220325 ◽

2021 ◽

Vol 22 (3) ◽

Author(s):

Mustafa Al-Dossary ◽

SHAYMAA S. RAHEEM ◽

MAYSOON K. ALMYAH

Keyword(s):

Molecular Analysis ◽

Molecular Identification ◽

Agricultural Soil ◽

Phenotypic Characteristics ◽

The Family ◽

First Time

Abstract. Al-Dossary MA, Raheem SS, Almyah MK. 2021. Molecular identification of five species of family Chaetomiaceae (Sordariomycetes, Ascomycota) from Iraqi soil. Biodiversitas 22: 1277-1284. Five ascomycetes fungi within the family Chaetomiaceae (Sordariomycetes, Ascomycota) were isolated from Iraqi agricultural soil and identified by phenotypic characteristics and molecular analysis. Chaetomium ascotrichoides and C. subaffine are reported for the first time from the Iraqi mycobiota. Amesia atrobrunnea, Collariella bostrychodes and Ovatospora brasiliensis were previously reported from Iraq as Chaetomium atrobrunneum, C. bostrychodes, and C. brasiliense respectively. All reported species were provided with brief characterizations and photographs

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DNA-based molecular identification of Urnula mediterranea (Ascomycota, Pezizales) collected in Central Serbia

Kragujevac Journal of Science ◽

10.5937/kgjsci2143053a ◽

2021 ◽

pp. 53-62

Author(s):

Dejan Arsenijević ◽

Stefan Blagojević ◽

Nevena Planojević ◽

Aleksandra Nikezić ◽

Dejan Vidanović ◽

...

Keyword(s):

Genetic Markers ◽

Molecular Systematics ◽

Molecular Identification ◽

Dna Sequences ◽

Morphological Characteristics ◽

Its Region ◽

28S Rrna ◽

Dna Molecules ◽

Identification Of Species ◽

First Time

Molecular systematics is a branch of systematics that analyzes genetic markers for the classification of organisms. Genetic markers include target sequences of molecules DNA, RNA, and amino acids. The most often used genetic markers are DNA markers, localized on DNA molecules. The study aim was DNA-based molecular identification of species Urnula mediterranea (M. Carbone, Agnello & Baglivo) M. Carbone, Agnello & P. Alvarado (2013), by using molecular systematics methods. This species was recently discovered for the first time in Central Serbia and determined only based on morphological characteristics. For the first time, U. mediterranea sampled in Serbia, was identified with DNA molecular markers. Extraction of DNA molecules was performed from the fruiting body of U. mediterranea. ITS region and the part of the gene for 28S rRNA were amplified and sequenced by the Sanger method. Comparing the analyzed sequences with the sequences from the database, a match of 99.72% was found for the ITS region and 100% for the part of the gene for 28S rRNA. By molecular identification, it has been established that the sample belongs to the species U. mediterranea. Both analyzed DNA sequences of U. mediterranea were deposited into the NCBI database.

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First report of Rinamba opacicollis Cameron (Hymenoptera: Braconidae, Doryctinae) in India as parasitoid of coffee stem borer, Xylotrechus quadripes (Chevrolat) (Coleoptera: Cerambycidae)

ENTOMON ◽

10.33307/entomon.v44i4.486 ◽

2020 ◽

Vol 44 (4) ◽

pp. 311-314

Author(s):

A. Roobakkumar ◽

H.G. Seetharama ◽

P. Krishna Reddy ◽

M.S. Uma ◽

A. P. Ranjith

Keyword(s):

Integrated Control ◽

Host Record ◽

Stem Borer ◽

Arabica Coffee ◽

First Report ◽

The World ◽

First Time

Rinamba opacicollis Cameron (Hymenoptera: Braconidae) was collected from Chikkamagaluru, Karnataka, India for the first time from the larvae of white stem borer, Xylotrechus quadripes Chevrolat infesting arabica coffee. Its role in the biological or integrated control of X. quadripes remains to be evaluated. White stem borer could be the first host record of this parasitoid all over the world.

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First Report of a Gall Midge Species (Diptera: Cecidomyiidae) Associated With Pistachios

Journal of Integrated Pest Management ◽

10.1093/jipm/pmaa022 ◽

2020 ◽

Vol 11 (1) ◽

Author(s):

Farshid O Sirjani ◽

Edwin E Lewis

Keyword(s):

Gall Midge ◽

Species Level ◽

Plant Tissues ◽

First Report ◽

Branch Dieback ◽

First Time ◽

Level Identification

Abstract A new dipterous pest is reported, for the first time, on commercial pistachios from Sirjan, Kerman province, Iran. The genus of the insect was determined to be Resseliella Seitner (Diptera: Cecidomyiidae). Adults are light brown to brown in color and 0.8–1.5 mm in length with females, generally, slightly larger than males. Females have an elongated ovipositor, which is characteristic of the genus. Larvae are orange in color, 2–3 mm in length in the later instars, feed under bark without inducing galls, and cause branch dieback on trees of various ages. Brown to black discolorations are observed on plant tissues under bark where the larvae feed. Infestations observed on current and the previous—year’s growths, ranged from 0.5 to 1.2 cm in diameter, and all located in outer branches. Dry leaves and fruit clusters on infested branches remain attached, which may be used to recognize infestation by the gall midge. Dark-colored, sunken spots with splits on the bark located at the base of the wilted sections of the shoots also are symptoms of Resseliella sp. larval activity. Species-level identification of the gall midge is currently underway.

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First Report of Echinococcus ortleppi in Free-Living Wild Boar (Sus scrofa) from Portugal

Microorganisms ◽

10.3390/microorganisms9061256 ◽

2021 ◽

Vol 9 (6) ◽

pp. 1256

Author(s):

Teresa Letra Mateus ◽

Maria João Gargaté ◽

Anabela Vilares ◽

Idalina Ferreira ◽

Manuela Rodrigues ◽

...

Keyword(s):

Wild Boar ◽

Internal Transcribed Spacer ◽

Sus Scrofa ◽

One Health ◽

Control Strategies ◽

Free Living ◽

First Report ◽

Wildlife Reservoirs ◽

First Time ◽

Echinococcus Granulosus Sensu Lato

Cystic echinococcosis (CE) is a zoonosis that is prevalent worldwide. It is considered endemic in Portugal but few studies have been performed on Echinococcus granulosus sensu lato and their hosts. In this study, CE cysts are reported for the first time in a free-living wild boar (Sus scrofa) in Portugal. The presence of the metacestodes in the liver of the wild boar was identified by morphological features, microscopic examination and molecular analysis. The sequencing of part of the DNA nuclear ribosomal internal transcribed spacer-1 (ITS-1) region revealed a G5 genotype that presently corresponds to Echinococcus ortleppi. This is the first report of E. ortleppi in Portugal and to the best of the authors’ knowledge, in Europe. These results suggest that wild boar may be a host of CE, namely, crossing the livestock–wildlife interface, which has important public health implications. Wildlife reservoirs must be taken into account as CE hosts and surveillance of game as well as health education for hunters should be implemented using a One Health approach, with implementation of feasible and tailor-made control strategies, namely, proper elimination of byproducts in the field.

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The First Record of Echinococcus ortleppi (G5) Tapeworms in Grey Wolf (Canis lupus)

Pathogens ◽

10.3390/pathogens10070853 ◽

2021 ◽

Vol 10 (7) ◽

pp. 853

Author(s):

Jacek Karamon ◽

Małgorzata Samorek-Pieróg ◽

Jacek Sroka ◽

Ewa Bilska-Zając ◽

Joanna Dąbrowska ◽

...

Keyword(s):

Small Intestine ◽

Molecular Identification ◽

Canis Lupus ◽

Sequence Comparison ◽

First Record ◽

Grey Wolf ◽

South Eastern ◽

Counting Technique ◽

Small Intestines ◽

First Time

The aim of this study is to confirm the presence and molecular identification of Echinococcus tapeworms in wolves from south-eastern Poland. An investigation was carried out on the intestines of 13 wolves from south-eastern Poland. The small intestines were divided into three equal segments. Each segment was separately examined using the sedimentation and counting technique (SCT). The detected Echinococcus tapeworms were isolated and identified by PCRs and sequencing (nad1 and cox1 genes). Additionally, DNA isolated from the feces of wolves positive for Echinococcus tapeworms was examined with two diagnostic PCRs. The intestines of one wolf were positive for E. granulosus s.l. when assessed by SCT; the intestine was from a six-year-old male wolf killed in a communication accident. We detected 61 adult tapeworms: 42 in the anterior, 14 in the middle, and 5 in the posterior parts of the small intestine. The PCRs conducted for cox1 and nad1 produced specific products. A sequence comparison with the GenBank database showed similarity to the deposited E. ortleppi (G5) sequences. An analysis of the available phylogenetic sequences showed very little variation within the species of E. ortleppi (G5), and identity ranged from 99.10% to 100.00% in the case of cox1 and from 99.04 to 100.00% in the case of nad1. One of the two diagnostic PCRs used and performed on the feces of Echinococcus-positive animals showed product specific for E. granulosus. This study showed the presence of adult E. ortleppi tapeworms in wolves for the first time.

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