scholarly journals Gene Transmission, Growth, and Exogeneous Growth Hormone Expression of G2 Transgenic Betta Fish (Betta imbellis)

2021 ◽  
Vol 13 (2) ◽  
pp. 181
Author(s):  
Nadia Ayuningthias ◽  
Hasan Nasrullah ◽  
Dinar Tri Soelistiyowati ◽  
Eni Kusrini ◽  
Alimuddin Alimuddin
Keyword(s):  
Growth Hormone ◽  
Growth Rate ◽  
Body Size ◽  
Transgenic Fish ◽  
Polymerase Chain Reaction Method ◽  
The Body ◽  
Pangasianodon Hypophthalmus ◽  
Polymerase Chain ◽  
The Brain ◽  
Transgene Detection

Highlight ResearchThe F2 of GH-transgenic B. imbellis was successfully producedThe transgene inheritance by the F2 fish was more than 90%The growth and body size of transgenic fish was significantly higher than controlF2 fish reached a larger body size in a shorter period compared to the F1 AbstractIn our previous research, we had successfully produced G0 and G1 Pangasianodon hypophthalmus growth hormone (PhGH) transgenic B. imbellis, native ornamental betta from Indonesia, which its giant-sized variant has valuable price for the breeders. The G0 and G1 transgenic (TG) fish showed higher growth rate and body size compared to the non-transgenic (NT) fish. The study was aimed to produce and evaluate the consistencies of transgene transmission and expression in G2 generation. The growth rate and body size between TG and NT fish was also compared. The G2 generation was produced using crosses between TG and NT G1 fish: ♂TG × ♀TG, ♂TG × ♀NT, ♂NT × ♀TG, and ♂NT ×♀ NT. Fish were reared for 12 weeks, and transgene detection was performed using the polymerase chain reaction method (PCR) on isolated DNA from the caudal fin clips. The endogenous and exogenous GH expression analysis was conducted using the quantitative real-time PCR (qPCR) method. The results showed that the inheritance of the GH transgene by the G2 fish was more than 90% in all transgenic crosses. Endogenous GH was expressed at the same levels in the brain of TG and NT fish, but the exogenous GH expression was highly detected only in the TG fish. The G2 transgenic fish had a higher specific growth rate, up to 31%, compared to the control. The body length of TG crosses were 23−35% higher and had 111−135% higher body weight compared to NT fish. These results showed a promising approached in mass-producing stable lines of giant-sized betta using the GH-transgenic technology.

scholarly journals Identification of Bovine Growth Hormone (BGH) Gene Polymorphism Using PCR-RFLP Method in Buffalo Bulls

2019 ◽  
pp. 1-8
Author(s):  
M. I. S. Hazlan Shah ◽  
M. S. Salisi ◽  
H. Wahid ◽  
M. S. Yahaya ◽  
Y. Rosnina ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Bubalus Bubalis ◽  
The Body ◽  
Bovine Growth Hormone ◽  
Agarose Gel ◽  
Single Polypeptide Chain ◽  
Pcr Rflp ◽  
Polymerase Chain ◽  
Single Polypeptide ◽  
Gland Development

Growth Hormone (GH) is a single polypeptide chain synthesised and secreted from anterior pituitary gland by somatroph cells. The product of GH gene hastens metabolism and promotes the growth of many organs and tissues especially bone, muscle and visceral organs. It also regulates growth, mammary gland development and lactation. Polymorphism in this gene is associated with increase in growth and development of many tissues in the body. Aim: The objective of this study was to investigate the polymorphism of bovine growth hormone (bGH) gene in buffalo bulls (Bubalus bubalis) using the PCR-RFLP (polymerase chain reaction–restriction fragment length polymorphism) technique. Design: Genomic DNA was extracted from a total of 10 bulls, consisting of Murrah – Swamp crossbred and pure Swamp buffalo bulls. A The 446 segment of the bGH gene was amplified. The DNA amplicons were detected in 2% agarose gel following 45 minutes of electrophoresis. They were thereafter digesting with AluI endonuclease restriction enzyme, and the digested DNA were detected in 2% agarose gel following electrophoresis for about 45minutes in all samples Results: Similar bands of approximately 300 and 146-bp each, with no variation, were detected in 2% agarose gel following electrophoresis in all the animals tested. Conclusion: Based on the Alu1 digestion result, all samples produced the same allele of the gene, with no polymorphism detected.

scholarly journals A skeletochronological estimate of age and growth in a large riparian frog from Madagascar (Anura, Mantellidae, Mantidactylus)

Herpetozoa ◽  
2019 ◽  
Vol 32 ◽  
pp. 39-44 ◽  
Author(s):  
Fabio M. Guarino ◽  
Angelica Crottini ◽  
Marcello Mezzasalma ◽  
Jasmin E. Randrianirina ◽  
Franco Andreone
Keyword(s):  
Growth Rate ◽  
Body Size ◽  
Sexual Size Dimorphism ◽  
The Body ◽  
Age And Growth ◽  
Growth Trajectories ◽  
Growth Coefficient ◽  
Size Dimorphism ◽  
Adult Females ◽  
Similar Growth

We characterized the body size (as snout-vent length), age, sexual size dimorphism, and growth rate in a population of one of the larger riparian frog from Madagascar (Mantidactylusgrandidieri) from a rainforest patch close to Vevembe, SE Madagascar. We identified a significant female-biased sexual size dimorphism. Age was estimated using phalangeal skeletochronology and was significantly higher in females than in males. Modal age class turned out to be 4 years in both sexes but a large percentage of adult females (75%) fell in the 5–6 years-old classes, while no male exceeded 4 years. We here report M.grandidieri as a medium-long-lived anuran species. Von Bertalanffy’s model showed similar growth trajectories between the sexes although the growth coefficient in females (k = 0.335) was slightly but not significantly higher than in males (k = 0.329).

scholarly journals How Precise are Size-Based Age Estimations in the Sand Lizard (Lacerta Agilis)?

2011 ◽  
Vol 56 (1-4) ◽  
pp. 11-17 ◽  
Author(s):  
Bartosz Borczyk ◽  
Łukasz Paśko
Keyword(s):  
Growth Rate ◽  
Body Size ◽  
Life Span ◽  
Age Estimation ◽  
The Body ◽  
Average Error ◽  
Ecological Studies ◽  
Age Classes ◽  
Sand Lizard ◽  
Lacerta Agilis

How Precise are Size-Based Age Estimations in the Sand Lizard (Lacerta Agilis)?Reptiles show a positive correlation between age and body size and it is common practice to infer the age of an animal from its size. However, the growth rate often differs between individuals, thus such practice may lead to false conclusions. Because age of an animal is a very important factor in many ecological studies, it should be determined with a minimum of error. Here, we compare the body size distribution among different age classes of the sand lizard (Lacerta agilis) to infer if it is possible to correctly determine their age on the basis of the body length. Our results show that the average error in age estimation on the basis of the lizard size is 1.36 year which is approximately 1/3 the average sand lizard life span.

The interplay between resource supply and demand determines the influence of predation on prey body size

2016 ◽  
Vol 73 (4) ◽  
pp. 709-715 ◽  
Author(s):  
John P. DeLong ◽  
Matthew Walsh
Keyword(s):  
Growth Rate ◽  
Body Size ◽  
Body Mass ◽  
Supply And Demand ◽  
Interspecific Variation ◽  
The Body ◽  
Growth Responses ◽  
Resource Demand ◽  
Growth Constants ◽  
Sd Model

Predation has been shown to either increase or decrease the body mass of fish, as well as cause variable changes in growth rate. The mechanisms underlying these contrasting responses are not well understood. Here we compared intraspecific body size and growth responses to predation against a backdrop of 2006 estimates of asymptotic mass and growth constants (i.e., von Bertalanffy parameters) across species. We show that intraspecific responses can be quite large relative to interspecific variation and confirm that the magnitude and direction of body size responses is variable. We then employed the supply–demand (SD) model of body mass evolution to explore how predator-induced changes in resource demand or supply could alter body mass. The SD model predicts that any combination of increasing or decreasing body mass and increasing or decreasing growth rate is possible when predation risk is increased, which is consistent with the literature. Finally, we use three case studies to illustrate how the interplay of resource supply and resource demand determines the actual body mass and growth rate response to predation.

scholarly journals Modelling the growth of the brown frog (Rana dybowskii)

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e4587 ◽  
Author(s):  
Qing Tong ◽  
Xiao-peng Du ◽  
Zong-fu Hu ◽  
Li-yong Cui ◽  
Hong-bin Wang
Keyword(s):  
Growth Rate ◽  
Body Weight ◽  
Body Size ◽  
Management Strategies ◽  
Growth Period ◽  
The Body ◽  
Order Polynomial ◽  
Polynomial Models ◽  
Brown Frog ◽  
Rana Dybowskii

Well-controlled development leads to uniform body size and a better growth rate; therefore, the ability to determine the growth rate of frogs and their period of sexual maturity is essential for producing healthy, high-quality descendant frogs. To establish a working model that can best predict the growth performance of frogs, the present study examined the growth of one-year-old and two-year-old brown frogs (Rana dybowskii) from metamorphosis to hibernation (18 weeks) and out-hibernation to hibernation (20 weeks) under the same environmental conditions. Brown frog growth was studied and mathematically modelled using various nonlinear, linear, and polynomial functions. The model input values were statistically evaluated using parameters such as the Akaike’s information criterion. The body weight/size ratio (Kwl) and Fulton’s condition factor (K) were used to compare the weight and size of groups of frogs during the growth period. The results showed that the third- and fourth-order polynomial models provided the most consistent predictions of body weight for age 1 and age 2 brown frogs, respectively. Both the Gompertz and third-order polynomial models yielded similarly adequate results for the body size of age 1 brown frogs, while the Janoschek model produced a similarly adequate result for the body size of age 2 brown frogs. The Brody and Janoschek models yielded the highest and lowest estimates of asymptotic weight, respectively, for the body weights of all frogs. TheKwlvalue of all frogs increased from 0.40 to 3.18. TheKvalue of age 1 frogs decreased from 23.81 to 9.45 in the first four weeks. TheKvalue of age 2 frogs remained close to 10. Graphically, a sigmoidal trend was observed for body weight and body size with increasing age. The results of this study will be useful not only for amphibian research but also for frog farming management strategies and decisions.

Bergmann’s rule for Neomys fodiens in the middle of the distribution range

2014 ◽  
Vol 9 (12) ◽  
pp. 1147-1154 ◽  
Author(s):  
Linas Balčiauskas ◽  
Laima Balčiauskienė ◽  
Uudo Timm
Keyword(s):  
Body Size ◽  
The Body ◽  
Distribution Range ◽  
Bergmann’S Rule ◽  
Bergmann's Rule ◽  
Range Analysis ◽  
Skull Size ◽  
Skull Measurements ◽  
The Brain ◽  
Neomys Fodiens

AbstractThe body size of Palearctic Sorex shrews decreases at higher latitudes, and as such the Bergmann’s rule does not work. However, no analysis has ever been done for water shrew (Neomys fodiens) in the middle of distribution range. Analysis of available literature data showed that some body and skull measurements of N. fodiens are negatively correlated to latitude. Measurements of 158 water shrews from Estonia and Lithuania were also analyzed with respect to the short scale latitudinal pattern. We found that populations are separated (Wilk’s lambda = 0.363, p<0.0001). Differences are related to PC1 (skull size), explaining 49.80% of the variance and PC2 (body size), explaining 10.06% of the variance. Estonian shrews are smaller in their body and skull (most differences significant) and their skulls are relatively shorter and wider in the area of the brain case. Thus, the negative correlation of body and skull size to latitude in N. fodiens is applicable even over quite short latitudinal distances. Further analysis of diagnostic characters between N. fodiens and N. anomalus is required.

scholarly journals Detection of Transgene in Tilapia (Oreochromis niloticus) by Polymerase Chain Reaction (PCR) Technique

2016 ◽  
Vol 8 (2) ◽  
pp. 47-49
Author(s):  
PC Ray ◽  
MS Alam ◽  
MS Islam
Keyword(s):  
Growth Hormone ◽  
Polymerase Chain Reaction ◽  
Oreochromis Niloticus ◽  
Gene Sequence ◽  
Transgenic Fish ◽  
Specific Primer ◽  
Chain Reaction ◽  
Gh Gene ◽  
Polymerase Chain ◽  
Transgenic Tilapia

Polymerase Chain Reaction (PCR) technique using specific primer can be used to detect transgenes. The present study was undertaken to detect salmon growth hormone (GH) gene in transgenic tilapia (Oreochromis niloticus) by PCR. DNA was extracted from F1 Tilapia generated by crossing transgenic parents. Two primers were designed to amplify a part of the region of GH gene sequence, which was used to make transgenic tilapia. To confirm the specificity of the selected primer, PCR was performed on diluted DNAs, extracted from tilapia fin tissues. GH transgene sequences (1500 bp) were successfully amplified from transgenic fish in this study. The specificity of the primers was found to be high in detecting the salmon GH transgenes. The PCR-based method therefore, could be used for fast and easy screening of transgenic fish for this gene.J. Environ. Sci. & Natural Resources, 8(2): 47-49 2015

scholarly journals Body Representation in Anorexia Nervosa

2021 ◽  
Author(s):  
Anouk Keizer ◽  
Manja Engel
Keyword(s):  
Anorexia Nervosa ◽  
Body Size ◽  
Body Representation ◽  
The Body ◽  
Size Perception ◽  
Tactile Stimuli ◽  
Body Experiences ◽  
Sensory Modalities ◽  
Multisensory Information ◽  
The Brain

Anorexia nervosa (AN) is an eating disorder that mainly affects young women. One of the most striking symptoms of this disorder is the distorted experience of body size and shape. Patients are by definition underweight, but experience and perceive their body as bigger than it in reality is. This body representation disturbance has fascinated scientists for many decades, leading to a rich and diverse body of literature on this topic. Research shows that AN patients do not only think that their body is bigger than reality, and visually perceive it as such, but that other sensory modalities also play an important role in oversized body experiences. Patients for example have an altered (enlarged) size perception of tactile stimuli, and move their body as if it is larger than it actually is. Moreover, patients with AN appear to process and integrate multisensory information differently than healthy individuals, especially in relation to body size. This leads to the conclusion that the representation of the size of the body in the brain is enlarged. This conclusion has important implications for the treatment of body representation disturbances in AN. Traditionally treatment of AN is very cognitive in nature, it is possible however that changed cognitions with respect to body size experiences do not lead to actual changes in metric representations of body size stored in the brain. Recently a few studies have been published in which a multisensory approach in treatment of body representation disturbance in AN has been found to be effective in treating this symptom of AN.

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