scholarly journals PENGARUH VARIASI ZAT PENGATUR TUMBUH IAA, BAP, KINETIN TERHADAP METABOLIT SEKUNDER KALUS SIRIH HITAM (Piper betle L. Var Nigra)

2019 ◽  
Vol 4 (2) ◽  
pp. 121
Author(s):  
Junairiah Junairiah ◽  
Nurul Sofi Amalia ◽  
Yosephine Sri Wulan Manuhara ◽  
Ni’matuzzahroh Ni’matuzahroh ◽  
Lilis Sulistyorini
Keyword(s):  
Gas Chromatography ◽  
Acetic Acid ◽  
Secondary Metabolite ◽  
Mass Spectra ◽  
Indole Acetic Acid ◽  
Culture Method ◽  
Piper Betle ◽  
Combination Treatments ◽  
Tissue Culture Method ◽  
Benzyl Amino Purine

AbstrakPiper betle L. var Nigra (sirih hitam)  merupakan salah satu jenis tanaman obat yang berpotensi untuk dikembangkan dengan metode kultur jaringan. Penelitian ini bertujuan untuk mengetahui pengaruh variasi zat pengatur tumbuh Indole Acetic Acid (IAA), Benzyl Amino Purine (BAP), Kinetin terhadap senyawa metabolit sekunder  yang terdapat kalus sirih hitam. Eksplan daun dari sirih hitam ditanam pada medium Murashige dan Skoog dengan 4 perlakuan kombinasi (I1,0K1,0; I1,0K1,5; I1B1,5; I0,5B0,5). Kalus dipelihara selama 8 minggu. Simplisia kalus sirih hitam di maserasi menggunakan pelarut metanol dan diidentifikasi menggunakan Gas Chromatography Mass Spectra (GCMS). Berdasarkan analisis GCMS menunjukkan bahwa senyawa metabolit sekunder dengan presentase tertinggi terdapat pada masing- masing perlakuan yaitu senyawa gamma sitosterol (14,88%) pada perlakuan I1,0K1,0 ; senyawa 14-Beta H Pregna (15,94%) pada perlakuan I1,0K1,5 serta senyawa beta-d-glucopyranoside (15,54%) pada perlakuan I0,5B0,5; dan senyawa beta-d-glucopyranoside (5,63%) pada perlakuan I1,0 B1,5. Katakunci: Kalus, Piper betle L. var Nigra, senyawa metabolit sekunder  AbstractPiper betle L. Var Nigra (black betel) is one type of medicinal plant that has the potential to be developed by tissue culture method. This study was aims to determine the effect of variations in growth regulator Indole Acetic Acid (IAA), Benzyl Amino Purine (BAP), and Kinetin on secondary metabolite compounds contained in black betel callus. The explant of black betel’s leaf was cultured on Murashige and Skoog medium with four combination treatments (I1.0K1.0; I1.0K1.5; I1B1.5; I0.5B0.5). The callus was maintained for eight weeks. Simplisia of black betel’s callus was macerated using methanol solvent and identified using Gas Chromatography Mass Spectra (GCMS). GCMS analysis showed that secondary metabolites with the highest percentage in each treatment were gamma sitosterol compound (14.88%) in treatment I1.0K1.0; 14-Beta H Pregna compound (15.94%) in I1.0K1.5 treatment and beta-d-glucopyranoside compound (15.54%) in I0.5B0.5 treatment; and beta-d-glucopyranoside compound (5.63%) in I1.0 B1.5 treatment.Keywords: Callus, Piper betle L. Var Nigra, secondary metabolite compounds

scholarly journals Effect of exogenous gibberellin on endogenous hormone and ginkgolide content in Ginkgo leaves

2020 ◽  
Vol 48 (1) ◽  
pp. 140-149
Author(s):  
Xian ZHANG ◽  
Jun ZHU ◽  
Shuiyuan CHENG ◽  
Wei-Wei ZHANG ◽  
Feng XU ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Abscisic Acid ◽  
Secondary Metabolites ◽  
Secondary Metabolite ◽  
Ginkgo Biloba ◽  
Indole Acetic Acid ◽  
Plant Secondary Metabolites ◽  
Test Material ◽  
Endogenous Iaa ◽  
The Given

Ginkgolide is one of the important secondary metabolites of Ginkgo biloba. The synthesis of plant secondary metabolites is influenced by exogenous phytohormones. In this study, ten-year-old ginkgo grafted seedlings were used as the test material, and the effects of 0, 2, 4, 6, 8, and 10 mmol L–1 gibberellin (GA3) on endogenous indole acetic acid (IAA), abscisic acid (ABA), GA3, and ginkgolide contents in ginkgo leaves were investigated. Results showed that exogenous GA3 treatment inhibited endogenous GA3, changed the contents of endogenous IAA and ABA in ginkgo leaves, and affected the accumulation of secondary metabolite ginkgolides. Among the given concentrations, the 4 mmol L–1 GA3 treatment could remarkably increase ginkgolide content. We found that the best harvesting period of ginkgo leaves were in late August and late September, in which the content of ginkgolides was the highest.

Isolation and identification of IAA producing endosymbiotic bacteria from Gracillaria corticata (J. Agardh)

2016 ◽  
Vol 5 (12) ◽  
pp. 5179
Author(s):  
Ilahi Shaik* ◽  
P. Janakiram ◽  
Sujatha L. ◽  
Sushma Chandra
Keyword(s):  
Acetic Acid ◽  
Culture Filtrate ◽  
Indole Acetic Acid ◽  
Shoot Growth ◽  
High Salinity ◽  
Saline Soils ◽  
Bacterial Strains ◽  
Isolation And Identification ◽  
Endosymbiotic Bacteria ◽  
Root And Shoot Growth

Indole acetic acid is a natural phytohormone which influence the root and shoot growth of the plants. Six (GM1-GM6) endosymbiotic bacteria are isolated from Gracilaria corticata and screened for the production of IAA out of six, three bacterial strains GM3, GM5 and GM6 produced significant amount of IAA 102.4 µg/ml 89.40 µg/ml 109.43 µg/ml respectively. Presence of IAA in culture filtrate of the above strains is further analyzed and confirmed by TLC. As these bacterial strains, able to tolerate the high salinity these can be effectively used as PGR to increase the crop yield in saline soils.

scholarly journals Characterization of Plant Growth-Promoting Traits and Inoculation Effects on Triticum durum of Actinomycetes Isolates under Salt Stress Conditions

Soil Systems ◽  
2021 ◽  
Vol 5 (2) ◽  
pp. 26
Author(s):  
Rihab Djebaili ◽  
Marika Pellegrini ◽  
Massimiliano Rossi ◽  
Cinzia Forni ◽  
Maria Smati ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Salt Stress ◽  
Plant Growth ◽  
Durum Wheat ◽  
Growth And Development ◽  
Indole Acetic Acid ◽  
Greenhouse Experiment ◽  
Hydrocyanic Acid ◽  
In Vitro Assays

This study aimed to characterize the halotolerant capability, in vitro, of selected actinomycetes strains and to evaluate their competence in promoting halo stress tolerance in durum wheat in a greenhouse experiment. Fourteen isolates were tested for phosphate solubilization, indole acetic acid, hydrocyanic acid, and ammonia production under different salt concentrations (i.e., 0, 0.25, 0.5, 0.75, 1, 1.25, and 1.5 M NaCl). The presence of 1-aminocyclopropane-1-carboxylate deaminase activity was also investigated. Salinity tolerance was evaluated in durum wheat through plant growth and development parameters: shoot and root length, dry and ash-free dry weight, and the total chlorophyll content, as well as proline accumulation. In vitro assays have shown that the strains can solubilize inorganic phosphate and produce indole acetic acid, hydrocyanic acid, and ammonia under different salt concentrations. Most of the strains (86%) had 1-aminocyclopropane-1-carboxylate deaminase activity, with significant amounts of α-ketobutyric acid. In the greenhouse experiment, inoculation with actinomycetes strains improved the morpho-biochemical parameters of durum wheat plants, which also recorded significantly higher content of chlorophylls and proline than those uninoculated, both under normal and stressed conditions. Our results suggest that inoculation of halotolerant actinomycetes can mitigate the negative effects of salt stress and allow normal growth and development of durum wheat plants.

Specificity of Phospholipid Binding to Indole Acetic Acid and Other Auxins

1974 ◽  
Vol 29 (1-2) ◽  
pp. 39-41 ◽  
Author(s):  
H. Veen
Keyword(s):  
Acetic Acid ◽  
Plant Growth ◽  
Indole Acetic Acid ◽  
Phospholipid Binding ◽  
Primary Action

Abstract Studies in vitro on the binding of phospholipids with IAA, α-NAA and some of their biologically inactive analogues demonstrate that the observed interaction between IA A and lecithin is not related to the primary action of the hormone in plant growth.

Isolation, Culture and Plant Regeneration From Protoplasts of Nicotiana debneyi

1980 ◽  
Vol 7 (6) ◽  
pp. 635 ◽  
Author(s):  
WR Scowcroft ◽  
PJ Larkin
Keyword(s):  
Acetic Acid ◽  
Plant Regeneration ◽  
Indole Acetic Acid ◽  
High Efficiency ◽  
Protoplast Culture ◽  
Callus Cultures ◽  
Dna Uptake ◽  
Mesophyll Protoplasts ◽  
Nicotiana Debneyi ◽  
Protoplast Division

Mesophyll protoplasts of two genetically distinct genotypes of N. debneyi were cultured with sustained division following a plating efficiency in excess of 50%. Fully fertile mature plants were regenerated from callus cultures derived from protoplasts. Shoots were induced in medium containing 1 mg/l 6-benzylaminopurine and 0.5 mg/I indole acetic acid. The repeatably high efficiency of protoplast culture was used to evaluate the quantitative effects of two drugs, kanamycin and trimethoprim, which effectively inhibited colony formation at concentrations of 100 and 50 �g/ml, respectively. An enhancer of DNA uptake, poly-L-ornithine, had virtually no effect on sustained protoplast division at a concentration of 7.5 �g/ml or less.

Sign in / Sign up

Export Citation Format

Share Document