An In Vitro Trial to Study the Effect of Inclusion of Different Levels of Coconut Oil on Sheep Rumen Methanogenesis and Fermentation Processes

2014 ◽  
Vol 2 (6) ◽  
pp. 49 ◽  
Author(s):  
Tisa Mukharji ◽  
Aakash Srivastava ◽  
Meera Srivastava
Keyword(s):  
Coconut Oil ◽  
Fermentation Processes ◽  
Sheep Rumen ◽  
Different Levels

scholarly journals Effect of vitex agnus-castus on sheep rumen fluids using an in vitro gas production method

2015 ◽  
Author(s):  
Sibel S Önenç
Keyword(s):  
Organic Matter ◽  
Production Method ◽  
Gas Production ◽  
Net Energy ◽  
Alfalfa Hay ◽  
In Vitro Gas Production ◽  
Agnus Castus ◽  
Sheep Rumen ◽  
Different Levels

The objective of this study was to evaluate the effects of different levels of Vitex agnus-castus (VAC) on in vitro gas production (GP), organic matter digestibility (OMD) and net energy lactation (NEL) using an in vitro gas-production method. Two rumen-fistulated sheep were used in the experiment. The sheep were fed 60% alfalfa hay and 40% concentrate feed twice daily. Five different levels of VAC were added to the concentrate (CON) to produce 200 mg DM (CON - without VAC; 1 - 2 mg VAC+198 mg CON; 2 - 4 mg VAC+196 mg CON; 3 - 6 mg VAC+194 mg CON; 4 - 8 mg VAC+192 mg CON; 5 - 10 mg VAC+190 mg CON). The volume of gas produced was recorded at 2, 4, 8, 12, and 24 h after incubation. The results showed GP, OMD and NEL contents with no significant changes even as the level of VAC were added to the CON. VAC groups affected the rapidly degraded fractions; however, did not affect the slowly degraded fractions.

scholarly journals Effect of vitex agnus-castus on sheep rumen fluids using an in vitro gas production method

2015 ◽  
Author(s):  
Sibel S Önenç
Keyword(s):  
Organic Matter ◽  
Production Method ◽  
Gas Production ◽  
Net Energy ◽  
Alfalfa Hay ◽  
In Vitro Gas Production ◽  
Agnus Castus ◽  
Sheep Rumen ◽  
Different Levels

The objective of this study was to evaluate the effects of different levels of Vitex agnus-castus (VAC) on in vitro gas production (GP), organic matter digestibility (OMD) and net energy lactation (NEL) using an in vitro gas-production method. Two rumen-fistulated sheep were used in the experiment. The sheep were fed 60% alfalfa hay and 40% concentrate feed twice daily. Five different levels of VAC were added to the concentrate (CON) to produce 200 mg DM (CON - without VAC; 1 - 2 mg VAC+198 mg CON; 2 - 4 mg VAC+196 mg CON; 3 - 6 mg VAC+194 mg CON; 4 - 8 mg VAC+192 mg CON; 5 - 10 mg VAC+190 mg CON). The volume of gas produced was recorded at 2, 4, 8, 12, and 24 h after incubation. The results showed GP, OMD and NEL contents with no significant changes even as the level of VAC were added to the CON. VAC groups affected the rapidly degraded fractions; however, did not affect the slowly degraded fractions.

scholarly journals Effect of Tannin Content in Horse Bean on Rumen Fermentation in vitro

2010 ◽  
Vol 79 (2) ◽  
pp. 217-224
Author(s):  
Wojciech Zawadzki ◽  
Albert Czerski ◽  
Edyta Wincewicz ◽  
Jan Gnus ◽  
Agnieszka Balcerzak ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Fermentation Process ◽  
Rumen Content ◽  
Bean Seed ◽  
Tannin Content ◽  
Fermentation Processes ◽  
Small Doses ◽  
Sheep Rumen ◽  
Ph Decrease

The objective of the experiments was to demonstrate the influence of low- and high-tannin horse bean on the selected fermentation indicators. The sheep rumen content with the addition of 1, 2 and 5 g of high- and low-tannin horse bean was incubated in vessels of 250 ml volume. A significant increase of ammonia, lactic acid, CO2 and methane as well as pH decrease in samples with addition of horse bean seed was demonstrated. While comparing high- and low-tannin horse bean varieties, it was observed that in the case of small doses of 1 and 2 g a higher intensity of fermentation takes place in the samples with the addition of high-tannin horse bean. In the case of larger doses of 5 g per sample, a higher intensity of fermentation takes place in the samples with the addition of low-tannin horse-bean. A probable reason for the decrease in the fermentation process intensity in the samples with the addition of 5 g of high-tannin horse bean is a high content of tannins, which reduce fermentation processes.

Immunoenzymatic demonstration of the simultaneous presence of transferrin, hemopexin and albumin in a same hepatocyte and their ultrastructural localization

1978 ◽  
Vol 36 (2) ◽  
pp. 88-89
Author(s):  
M. Kraemer ◽  
J. Foucrier ◽  
J. Vassy ◽  
M.T. Chalumeau
Keyword(s):  
Plasma Proteins ◽  
Rat Hepatocytes ◽  
Ultrastructural Localization ◽  
Carrier Proteins ◽  
Normal Cells ◽  
Adult Rat ◽  
Adult Rat Hepatocytes ◽  
Monospecific Antisera ◽  
Different Levels

Some authors using immunofluorescent techniques had already suggested that some hepatocytes are able to synthetize several plasma proteins. In vitro studies on normal cells or on cells issued of murine hepatomas raise the same conclusion. These works could be indications of an hepatocyte functionnal non-specialization, meanwhile the authors never give direct topographic proofs suitable with this hypothesis.The use of immunoenzymatic techniques after obtention of monospecific antisera had seemed to us useful to bring forward a better knowledge of this problem. We have studied three carrier proteins (transferrin = Tf, hemopexin = Hx, albumin = Alb) operating at different levels in iron metabolism by demonstrating and localizing the adult rat hepatocytes involved in their synthesis.Immunological, histological and ultrastructural methods have been described in a previous work.

scholarly journals 49 Effects of in-vitro digestibility in cannulated steers when supplemented different levels of glycerol

2020 ◽  
Vol 98 (Supplement_2) ◽  
pp. 55-56
Author(s):  
Noheli Gutierrez ◽  
Jamie A Boyd
Keyword(s):  
Dry Matter ◽  
Cold Water ◽  
Rumen Fluid ◽  
Latin Square ◽  
Optimal Level ◽  
Nutrient Digestibility ◽  
In Vitro Digestibility ◽  
Study Results ◽  
Different Levels

Abstract A study was conducted to evaluate effects of increasing concentration of food grade glycerol on rumen environment and nutrient digestibility. Three ruminally cannulated Jersey steers were used in this study. The study was conducted from March to May 2019. Experimental design was a 3x3 Latin square with a 2wk adjustment period followed by a 1wk collection period. Diet was coastal bermudagrass hay based. Different forage types were introduced in the incubation process to evaluate digestibility. Glycerol was administered once a day at 0, 15, or 20% of DMI (dry matter intake). dNDF (digestible NDF) and dDM (digestible dry matter) was determined using an ANKOM Daisy II incubator inoculated with 200g fresh rumen fluid and incubated for 12, 24, 48 and 72 h at 39°C. Each vessel contained ground forage samples in filter bags in triplicate. After incubation, filter bags were rinsed with cold water and dried for 24h in a 55°C forced air oven. Data were analyzed using the Proc MIXED procedure of SAS version 9.4. There was no difference dNDF in effect of different levels of glycerol between forage types by diet. But a numerical tendency was observed that dNDF was decreased at 20% inclusion rates in comparison to 0 and 15% inclusion of glycerol in the diet. Neither steer nor run was significantly different in the study. However as expected digestibility over time was significantly different (P < 0.001). A significant increase was observed in DMI with the increased levels of glycerol in the diet (P = 0.003), both the 15% and 20% levels of glycerol increased in DMI in comparison to the control (0%). It appears based on these study results that digestibility may be inhibited, as levels of dietary glycerol increase in the diet and more work needs to be done to find the optimal level of glycerol supplementation.

Antimicrobial and antibiofilm activities of meropenem loaded-mesoporous silica nanoparticles against carbapenem-resistant Pseudomonas aeruginosa

2021 ◽  
pp. 088532822110038
Author(s):  
Mohammad Yousef Memar ◽  
Mina Yekani ◽  
Hadi Ghanbari ◽  
Edris Nabizadeh ◽  
Sepideh Zununi Vahed ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Mesoporous Silica ◽  
Silica Nanoparticles ◽  
Mesoporous Silica Nanoparticles ◽  
Carbapenem Resistant ◽  
Toxicity In Vitro ◽  
Different Levels

The aims of the present study were the determination of antimicrobial and antibiofilm effects of meropenem-loaded mesoporous silica nanoparticles (MSNs) on carbapenem resistant Pseudomonas aeruginosa ( P. aeruginosa) and cytotoxicity properties in vitro. The meropenem-loaded MSNs had shown antibacterial and biofilm inhibitory activities on all isolates at different levels lower than MICs and BICs of meropenem. The viability of HC-04 cells treated with serial concentrations as MICs and BICs of meropenem-loaded MSNs was 92–100%. According to the obtained results, meropenem-loaded MSNs display the significant antibacterial and antibiofilm effects against carbapenem resistant and biofilm forming P. aeruginosa and low cell toxicity in vitro. Then, the prepared system can be an appropriate option for the delivery of carbapenem for further evaluation in vivo assays.

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