scholarly journals In vitro Efficacy of Bioagents and Fungicides on the Management of Dry Root Rot of Cluster Bean (Macrophomina phaseolina)

Author(s):  
Ran vijay ◽  
Prashant Mishra ◽  
Abhishek Kumar ◽  
Shashank Mishra
2016 ◽  
Vol 8 (9) ◽  
pp. 205
Author(s):  
Asma Hanif ◽  
Shahnaz Dawar

<p>The aim of this study was to assess fungicidal potential of homeopathic globules namely <em>Thuja occidentalis</em> and <em>Arnica montana</em> (30C) on plant growth and root infecting fungi particularly <em>Rhizoctonia solani</em>,<em> Fusarium</em> spp. and <em>Macrophomina phaseolina.</em> Both <em>in vitro</em> and <em>in vivo</em> experiments had found positive results in the suppression of root rot fungi. Investigation on present study showed that <em>A. montana</em> and <em>T. occidentalis</em> globules (100, 75 and 50% v/w concentrations) reduced disease intensity caused by root rot pathogens and improved growth of test plants, but it produces negative effects on leguminous test crops in which nodules were failing to produce.</p>


2014 ◽  
Vol 13 ◽  
pp. 70-74 ◽  
Author(s):  
S. Balamurugan

The aqueous extracts of leaf of Citrus aurantifolia L were assessed in vitro for inhibitory activity against Macrophominaphaseolina isolated from dry root rot specimens of Gingelly. The antifungal activity was determined by poison food technique. The extracts have shown dose dependent inhibition of mycelial growth of test fungi. The extracts were more effective in inhibiting Macrophominaphaseolina. The extracts of Citrus aurantifolia were found effective against Gingelly dry root rot pathogens. Further field experiments are to be carried out to recommend the extracts against the disease.


2020 ◽  
Vol 15 (2) ◽  
pp. 96-100
Author(s):  
S. Malathi

Actinomycetes were tested for their antagonistic activity against Fusarium udum and Macrophomina phaseolina under in vitro condition. Among the tested isolates of Actinomycetes AC (5) reported highest 82.85 per cent reduction of mycelial growth of Fusarium udum and 85.13 per cent reduction of mycelial growth of Macrophomina phaseolina. In the field experiment, five treatments were tested for the management of wilt and dry root rot disease, T3- ST+ SA with Actinomycetes (AC 5) significantly recorded 71.92 and 70.38 per cent reduction of the wilt and dry root rot diseases, respectively. These biocontrol agents were used an alternative to the chemical fungicide for controlling the wilt and dry root rot incidence and enhanced the plant growth parameters and there by increased yield in redgram.


Author(s):  
P. T. Sharavanan ◽  
V. K. Satya ◽  
M. Rajesh

Root rot of mung bean [Vigna radiata (L.) Wilczek var. radiata] is major disease and claims huge yield loss if they occur in the field. The pathogen is basically soil borne and survivability may vary depends on soil condition. The fungicide chemicals are available to manage the disease; however, the biocontrol agents are nowadays available for the disease management and the microbial activity of the biocontrol agents is influenced by existing soil condition including soil pH. Hence, a study was conducted to find out the halo tolerance capacity of the biocontrol agents against root rot disease in salt affected soils under in vitro, in vivo and field condition. The root rot pathogen Macrophomina phaseolina was isolated from infected root. Efficacy of biocontrol agents against growth of M. phaseolina was assessed in vitro. The results revealed that TNAU strain of Bacillus subtilis reduced the mycelial growth of the M. phaseolina significantly when media supplemented with NaCl at 5% (1.4 cm), 7.5% (1.5 cm), 10% (1.6cm) and 12.5% (1.6 cm) and without NaCl (1.2 cm) and similar trend of reduction also expressed by BCA1 strain of B. subtilis, Pseudomonas fluorescens and Trichoderma viride under in vitro. The performance of the biocontrol agents against the pathogen is slightly reduced when media supplemented with NaCl. The reduction of mycelia weight of M.phaeolina was more in media added with TNAU strain of B.subtilis and the performance of TNAU strain of B.subtilis on reduction of mycelial weight of M.phaseolina is reduced when the broth added with NaCl at 5% (3.15g), 7.5% (3.25g), 10% (3.32g) and 12.5%(3.65g) level and which is followed by P. fluorescens, BCA 1 strain of B. subtilis and Trichoderma viride. Under pot culture conditions, the effect of talc formulated biocontrol agents and challenge inoculation with pathogen was assessed against root rot incidence. It was found that the soil application of TNAU strain of B.subtilis performed better in reducing the root rot incidence at pH of 7.0 (2.37%), 7.5 (4.50%), 8.0 (5.53%) and 8.7 (6.57%) and followed by BCA 1 of B.subtilis in all pH level. Among the biocontrol agents, TNAU strain of B.subtilis applied as seed as well as soil application expressed more population in the rhizosphere in all pH level. The biocontrol agents applied as soil application had more populations of the agents in the soil when compared to seed treatment. The halo tolerance performance of the biocontrol agents was also assessed under field condition in pH of 7.5 and 8.7 during 2019-20 and 2020-21. It was found that the minimum root rot incidence and maximum yield was observed from soil application of TNAU strain of B subtilis at 2.5 kg/ha but the effect is on par with soil application of BCA1 strain of B.subtilis at 2.5 kg/ha.


Author(s):  
Monali A. Deshmukh ◽  
R. M. Gade ◽  
Y. K. Belkar ◽  
Mina D. Koche

The potential of soil amendaments, bioagents and biofertilizers was evaluated for management of root rot caused by Macrophomina phaseolina (Rhizoctonia bataticola). Bioagents and biofertilizers i.e.Trichoderma harzianum, Pseudomonas fluorescens, Phosphate solubilizing bacteria and Rhizobium phaseoli exhibited significant inhibition of Rhizoctonia bataticola. Maximum inhibition was achieved by Trichoderma harzianum i.e. 80.5%. Compatibility amongst bioagents and biofertilizers were tested in vitro. Amongst them Trichoderma harzianum and Rhizobium phaseoli were found compatible to each other. In green house, seed treatment with the bioagents and biofertilizers and also application of soil amendments recorded good number of nodules. Application of FYM and seed treatment with R. phaseoli at 25 g/kg + P. fluorescens at 10 g/kg recorded maximum number of nodules (15.3). Different seed treatments with soil amendments were used for controlling root rot incidence. Among them (T6) FYM with seed treatment of T. harzianum 4 g/kg seed + PSB at 25 g/kg seed recorded the lowest root rot incidence (26.0%) followed by (T3) R. phaseoli at 25 g/kg seed + P. fluorescens at 10 g/kg seed which recorded 32.0% root rot incidence whereas, maximum incidence of the disease was recorded in the control (83.3%).


Author(s):  
Mariam H. Ishak ◽  
Marzouk R. Abdel-Latif ◽  
Harby M. Abd Alla ◽  
Mohamed M. N. Shaat

Thyme (Thymus vulgaris L.) is a medicine and aromatic plant, have a great economic important due to the great income that it provides and other diversified usages. Damping-off, root rot and wilt symptoms were observed in field thyme grown in Maghagha and Beni Mazar districts, Minia Governorate, Egypt, during November to March, 2016-2017. Survey, sampling and pathogenicity tests were carried out. Fusarium semitectum, F. solani, Rhizoctonia solani and Macrophomina phaseolina were the dominant pathogens causing thyme damping off, root rot and wilt. The inhibitory effect of two resistance inducer substances (salicylic and ascorbic acids) three fungicides (Cure M 72% WP, Rizolex Ex-T 50% WP and Rolex 50% WP) on the growth of the most pathogenic fungi in vitro was investigated. Salicylic acid inhibited the growth of F. semitectum and R. solani completely at 200ppm but the growth of M. phaseolina was completely inhibited at 400ppm. The complete inhibition for the growth of all tested pathogens was occurred at 400 ppm Ascorbic acid. F. semitectum and R. solani were more sensitive to Rizolex T, while M. phaseolina was more sensitive to Cure-M, (causing the lowest diameter averages The results of the present investigation showed the possibility to use certain antioxidants as fungicides alternatives to control plant pathogenic fungi. According to the available literature, this is a first study on thyme root rot/wilt in Egypt.


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