scholarly journals The Development of Rapid, Accurate and Simple Identification Techniques for Bacterial Grain Rot (Burkholderia Glumae) on Rice

2018 ◽  
Vol 6 (12) ◽  
pp. 1-6 ◽  
Author(s):  
Ikhwana Aflaha ◽  
Baha ruddin ◽  
dan Untung Surapati
Keyword(s):  
Burkholderia Glumae ◽  
Identification Techniques ◽  
Bacterial Grain Rot

scholarly journals Incidence of Bacterial Grain Rot Disease, Identification, and Diversity of Burkolderia glumae in Some Rice Varieties in West Java

2020 ◽  
Vol 16 (1) ◽  
pp. 9-20
Author(s):  
Ani Widarti ◽  
Giyanto Giyanto ◽  
Kikin Hamzah Mutaqin
Keyword(s):  
16S Rrna ◽  
Burkholderia Glumae ◽  
West Java ◽  
Rice Varieties ◽  
Disease Identification ◽  
Rot Disease ◽  
Bacterial Grain Rot

Penyakit busuk bulir padi oleh bakteri Burkholderia glumae perlu diwaspadai termasuk di Jawa Barat sebagai salah satu sentra produksi padi nasional. Penelitian ini bertujuan untuk menentukan insidensi penyakit, identitas dan keragaman bakteri B. glumae pada beberapa varietas padi di Provinsi Jawa Barat. Pengamatan insidensi penyakit dan pengambilan sampel dilakukan di 9 kabupaten. Bakteri diisolasi dari bulir padi yang bergejala busuk bulir kemudian dilakukan uji biokimia dan fisiologi yang meliputi uji Gram serta uji pertumbuhan pada pH 4.8, dan NaCl 2%. Variasi fenotipik diamati dari warna koloni pada medium S-PG, produksi toksofalvin, respons hipersensitivitas pada daun tembakau, dan uji patogenisitas pada tanaman padi. Primer spesifik JLBgF/JLBgR dan primer universal 16S rRNA, yaitu 27F/1492R digunakan untuk menentukan identitas bakteri secara molekuler. Insidensi penyakit di lapangan berkisar antara 0–73.3%, tertinggi di Kecamatan Dawuan (Karawang) pada var. Mekongga. Berdasarkan uji biokimia dan fisiologi diperoleh 29 isolat terkonfirmasi sebagai B. glumae. Hasil pengamatan fenotipik menunjukkan 10 isolat tergolong koloni tipe A, 19 isolat tipe B; 25 isolat menghasilkan toksoflavin; 29 isolat menimbulkan respons hipersensitivitas pada daun tembakau dan gejala hawar pada tanaman padi. Identifikasi menggunakan primer spesifik dan universal membuktikan 29 isolat adalah B. glumae. Analisis keragaman genotip menunjukkan bahwa isolat KRCH-2 (Karawang) dan INCH-6 (Indramayu) memiliki hubungan kekerabatan yang dekat dengan B. glumae asal Cina dan Amerika

scholarly journals Identifikasi penyebab penyakit busuk bulir bakteri pada tanaman padi (Oryza sativa) dan pengendaliannya menggunakan isolat Bacillus spp. secara in vitro

2020 ◽  
Vol 1 (1) ◽  
pp. 14
Author(s):  
Siti Juli Isnaeni ◽  
Rachmi Masnilah
Keyword(s):  
Design Method ◽  
Severe Disease ◽  
Research Result ◽  
Resistance Mechanisms ◽  
Burkholderia Glumae ◽  
Bacillus Spp ◽  
Rot Disease ◽  
Disease Attack ◽  
Bacterial Grain Rot

Bacterial grain rot disease in rice plant is a new disease in Indonesia. The disease can reduce rice production up to 40% in severe disease attack. Morphological and physiological identification of these pathogens can be useful as a strategy of disease control. Control of this disease using Agent Biological Control is a right step and eco-friendly. The research was conducted in July until November 2018 at Plant Quarantine Laboratory of Agricultural Quarantine Center Surabaya. Design of experiment used on pathogenic identification is descriptively and analytically while in strategy of diseases control used are Randomized Complete Design method of single factor as many as 5 treatment of Bacillus spp. such as; Ba1: Bacillus spp. isolate 1, Ba2: Bacillus spp. isolate 2, Ba3: Bacillus spp. isolate 3, Ba4: Bacillus spp. isolate 4, and Ba5: Bacillus isolate spp. 5. Research result showed that the pathogen causing bacterial grain rot in Rice is Burkholderia glumae with bacterial characteristic are Gram negative, aerobic, not fluorescent on Kings B medium, mesophyll, isotonic, can hydrolysis arginine and can produce enzyme of gelatinase and (2) Bacillus spp. Isolate which consistent to inhibiting B. glumae in vitro is Ba-1 with a mean inhibition of 17.88 mm and a variety of resistance mechanisms, namely bacteriostatic and bactericidal Isolate of Bacillus spp Keywords: Bacterial grain rot rice; Bacillus spp; Burkholderia glumae

scholarly journals Applications of bacteriophages in controlling rice bacterial grain rot caused by Burkholderia glumae

2021 ◽  
Vol 13 (3) ◽  
pp. 17-22
Author(s):  
Tien Doan Thi Kieu ◽  
Tai Ngo Ngoc ◽  
Kaeko Kamei ◽  
Thi Thu Thuy Tran ◽  
Thi Thu Nga Nguyen
Keyword(s):  
High Efficacy ◽  
Burkholderia Glumae ◽  
Disease Reduction ◽  
Rot Disease ◽  
Different Levels ◽  
Bacterial Grain Rot

The study on the procedure of applicating bacteriophage (or phage) to prevent rice bacterial grain rot caused by Burkholderia glumae was conducted in the greenhouse conditions. The first experiment investigated the effect of different phage titers (i.e. 105 PFU/mL, 106 PFU/mL, 107 PFU/mL, 108 PFU/mL) in controlling bacterial grain rot of rice. The results showed that all four titers gave disease reduction with different levels, among these were the titer of 108PFU/mL expressed highest efficacy in disease reduction with the lowest percentage of infected grains compared to the rest treatments. The second experiment examined the effect of phage application times (i.e. spraying phage at 2 hours before pathogen inoculation, 2 hours before and 5 days after pathogen inoculation, and 5 days after pathogen inoculation) in suppressing bacterial grain rot disease. The results found that two treatments (i.e. one time spraying at 2 hours before pathogen inoculation, and two times spraying at 2 hours before and 5 days after pathogen inoculation) expressed high efficacy in reduction of grain rot disease through percentage of infected grains and improved yield parameter regarding rate of filled grains.

scholarly journals Evaluation of major Japanese rice cultivars for resistance to bacterial grain rot caused by Burkholderia glumae and identification of standard cultivars for resistance

2018 ◽  
Vol 68 (4) ◽  
pp. 413-419 ◽  
Author(s):  
Ritsuko Mizobuchi ◽  
Shuichi Fukuoka ◽  
Chikako Tsuiki ◽  
Seiya Tsushima ◽  
Hiroyuki Sato
Keyword(s):  
Rice Cultivars ◽  
Burkholderia Glumae ◽  
Bacterial Grain Rot

Metode Diplomatik dalam Mengidentifikasi Kandungan Isi Naskah Gulungan Berbahan Daluang Koleksi Candi Cangkuang

Panggung ◽  
2012 ◽  
Vol 22 (4) ◽  
Author(s):  
Tedi Permadi
Keyword(s):  
Direct Observation ◽  
Relevant Literature ◽  
Script Identification ◽  
Identification Techniques

ABSTRACTThis paper presents the results of the identification of rolled manuscripts made of daluang using diplomatic method. This method aims at getting the authenticity of the script based on the information that accompanies the text with the internal evidence contained in the manuscript. In terms of script identification techniques, diplomatic method utilizes direct observation techniques, assisted by other descriptions of contemporary manuscript as an evidence and support of the relevant literature. The use of diplomatic method in identifying rolled manuscripts produces the characteristics of the material, the literacy/language used in the text, and the editorial lapses contained in the text, but the identity of the author or the copyist and the time of the writing or copying manuscripts could not be found.Keywords: Manuscript identification, daluang, diplomatic method ABSTRAKTulisan ini menyajikan hasil identifikasi naskah gulungan berbahan daluang dengan menggunakan metode diplomatik. Metode diplomatik bertujuan untuk mendapatkan keaslian naskah berdasarkan informasi yang ada di dalam teks dengan bukti internal yang terkandung dalam naskah tersebut. Dalam hal teknik identifikasi naskah, metode diplomatik memanfaatkan teknik observasi langsung, dibantu dengan deskripsi dari naskah kontemporer lain sebagai bukti dan pendukung literatur yang relevan. Penggunaan metode diplomatik dalam mengidentifikasi naskah gulungan menghasilkan karakteristik material, huruf/bahasa yang digunakan dalam teks, dan penyimpangan editorial yang terkandung dalam teks, tetapi tidak bisa menemukan identitas penulis atau penyalin dan waktu penulisan atau penyalinan naskah.Kata kunci: Identifikasi naskah, daluang, metode diplomatik

Candida auris and Nosocomial Infection

2020 ◽  
Vol 21 (4) ◽  
pp. 365-373 ◽  
Author(s):  
Sweety Dahiya ◽  
Anil K. Chhillar ◽  
Namita Sharma ◽  
Pooja Choudhary ◽  
Aruna Punia ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Pathogenic Fungus ◽  
Control Measures ◽  
Whole Genome Sequence ◽  
Whole Genome ◽  
Drug Resistant ◽  
Candida Auris ◽  
Preventive Control ◽  
Identification Techniques ◽  
Sensitivity Profile

The existence of the multi-drug resistant (MDR) pathogenic fungus, Candida auris came to light in 2009. This particular organism is capable of causing nosocomial infections in immunecompromised persons. This pathogen is associated with consistent candidemia with high mortality rate and presents a serious global health threat. Whole genome sequence (WGS) investigation detected powerful phylogeographic Candida auris genotypes which are specialized to particular geological areas indicating dissemination of particular genotype among provinces. Furthermore, this organism frequently exhibits multidrug-resistance and displays an unusual sensitivity profile. Identification techniques that are commercialized to test Candida auris often show inconsistent results and this misidentification leads to treatment failure which complicates the management of candidiasis. Till date, Candida auris has been progressively recorded from several countries and therefore its preventive control measures are paramount to interrupt its transmission. In this review, we discussed prevalence, biology, drug-resistance phenomena, virulence factors and management of Candida auris infections.

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