scholarly journals Identifikasi penyebab penyakit busuk bulir bakteri pada tanaman padi (Oryza sativa) dan pengendaliannya menggunakan isolat Bacillus spp. secara in vitro

2020 ◽  
Vol 1 (1) ◽  
pp. 14
Author(s):  
Siti Juli Isnaeni ◽  
Rachmi Masnilah

Bacterial grain rot disease in rice plant is a new disease in Indonesia. The disease can reduce rice production up to 40% in severe disease attack. Morphological and physiological identification of these pathogens can be useful as a strategy of disease control. Control of this disease using Agent Biological Control is a right step and eco-friendly. The research was conducted in July until November 2018 at Plant Quarantine Laboratory of Agricultural Quarantine Center Surabaya. Design of experiment used on pathogenic identification is descriptively and analytically while in strategy of diseases control used are Randomized Complete Design method of single factor as many as 5 treatment of Bacillus spp. such as; Ba1: Bacillus spp. isolate 1, Ba2: Bacillus spp. isolate 2, Ba3: Bacillus spp. isolate 3, Ba4: Bacillus spp. isolate 4, and Ba5: Bacillus isolate spp. 5. Research result showed that the pathogen causing bacterial grain rot in Rice is Burkholderia glumae with bacterial characteristic are Gram negative, aerobic, not fluorescent on Kings B medium, mesophyll, isotonic, can hydrolysis arginine and can produce enzyme of gelatinase and (2) Bacillus spp. Isolate which consistent to inhibiting B. glumae in vitro is Ba-1 with a mean inhibition of 17.88 mm and a variety of resistance mechanisms, namely bacteriostatic and bactericidal Isolate of Bacillus spp Keywords: Bacterial grain rot rice; Bacillus spp; Burkholderia glumae

2001 ◽  
Vol 91 (3) ◽  
pp. 282-292 ◽  
Author(s):  
B. Cottyn ◽  
E. Regalado ◽  
B. Lanoot ◽  
M. De Cleene ◽  
T. W. Mew ◽  
...  

During the 1995 wet season, harvested rice seed was collected from farmers' fields at different locations in Iloilo, Philippines. Bacterial isolations from crushed seed yielded 428 isolates. The isolates were characterized by BOX-polymerase chain reaction fingerprinting of total genomic DNA and represented 151 fingerprint types (FPT). Most FPTs were found on a single occasion, although matching fingerprints for isolates from different samples also were found. Identifications were made by cellular fatty acid methyl ester analysis and additional use of Biolog GN/GP MicroPlates and API 20E/50CHE systems. The predominant bacteria were Enterobacteriaceae (25%), Bacillus spp. (22%), and Pseu-domonas spp. (14%). Other bacteria regularly present were identified as Xanthomonas spp., Cellulomonas flavigena, and Clavibacter michiganense. Of the total number of isolated bacteria, 4% exhibited in vitro antifungal activity against Rhizoctonia solani or Pyricularia grisea. Two percent of isolates were pathogens identified as Burkholderia glumae and Burkholderia gladioli. Five percent of isolates induced sheath necrosis on only 50 to 90% of inoculated plants and were related to Bacillus pumilus, Paenibacillus spp., Pseudomonas spp., and Pantoea spp.


2020 ◽  
Vol 16 (1) ◽  
pp. 9-20
Author(s):  
Ani Widarti ◽  
Giyanto Giyanto ◽  
Kikin Hamzah Mutaqin

Penyakit busuk bulir padi oleh bakteri Burkholderia glumae perlu diwaspadai termasuk di Jawa Barat sebagai salah satu sentra produksi padi nasional. Penelitian ini bertujuan untuk menentukan insidensi penyakit, identitas dan keragaman bakteri B. glumae pada beberapa varietas padi di Provinsi Jawa Barat. Pengamatan insidensi penyakit dan pengambilan sampel dilakukan di 9 kabupaten. Bakteri diisolasi dari bulir padi yang bergejala busuk bulir kemudian dilakukan uji biokimia dan fisiologi yang meliputi uji Gram serta uji pertumbuhan pada pH 4.8, dan NaCl 2%. Variasi fenotipik diamati dari warna koloni pada medium S-PG, produksi toksofalvin, respons hipersensitivitas pada daun tembakau, dan uji patogenisitas pada tanaman padi. Primer spesifik JLBgF/JLBgR dan primer universal 16S rRNA, yaitu 27F/1492R digunakan untuk menentukan identitas bakteri secara molekuler. Insidensi penyakit di lapangan berkisar antara 0–73.3%, tertinggi di Kecamatan Dawuan (Karawang) pada var. Mekongga. Berdasarkan uji biokimia dan fisiologi diperoleh 29 isolat terkonfirmasi sebagai B. glumae. Hasil pengamatan fenotipik menunjukkan 10 isolat tergolong koloni tipe A, 19 isolat tipe B; 25 isolat menghasilkan toksoflavin; 29 isolat menimbulkan respons hipersensitivitas pada daun tembakau dan gejala hawar pada tanaman padi. Identifikasi menggunakan primer spesifik dan universal membuktikan 29 isolat adalah B. glumae. Analisis keragaman genotip menunjukkan bahwa isolat KRCH-2 (Karawang) dan INCH-6 (Indramayu) memiliki hubungan kekerabatan yang dekat dengan B. glumae asal Cina dan Amerika


2021 ◽  
Vol 13 (3) ◽  
pp. 17-22
Author(s):  
Tien Doan Thi Kieu ◽  
Tai Ngo Ngoc ◽  
Kaeko Kamei ◽  
Thi Thu Thuy Tran ◽  
Thi Thu Nga Nguyen

The study on the procedure of applicating bacteriophage (or phage) to prevent rice bacterial grain rot caused by Burkholderia glumae was conducted in the greenhouse conditions. The first experiment investigated the effect of different phage titers (i.e. 105 PFU/mL, 106 PFU/mL, 107 PFU/mL, 108 PFU/mL) in controlling bacterial grain rot of rice. The results showed that all four titers gave disease reduction with different levels, among these were the titer of 108PFU/mL expressed highest efficacy in disease reduction with the lowest percentage of infected grains compared to the rest treatments. The second experiment examined the effect of phage application times (i.e. spraying phage at 2 hours before pathogen inoculation, 2 hours before and 5 days after pathogen inoculation, and 5 days after pathogen inoculation) in suppressing bacterial grain rot disease. The results found that two treatments (i.e. one time spraying at 2 hours before pathogen inoculation, and two times spraying at 2 hours before and 5 days after pathogen inoculation) expressed high efficacy in reduction of grain rot disease through percentage of infected grains and improved yield parameter regarding rate of filled grains.


2019 ◽  
Vol 8 (1) ◽  
pp. 21-29
Author(s):  
Arfan Abrar ◽  
Armina Fariani ◽  
Yopi Amalia

 ABSTRAKKualitas rumput tembaga merupakan hijauan dengan produksi melimpah tetapi belum banyak dimanfaatkan secara optimal sebagai pakan ternak. Oleh karena itu, perlu dikombinasi dengan daun singkong, lumpur sawit dan disuplementasi ragi Sc untuk meningkatkan nutrisi dari ransum yang berbahan dasar rumput kumpai tembaga. Penelitian ini menggunakan metode rancangan acak lengkap (RAL) dengan 4 perlakuan dan 4 ulangan. Perlakuan terdiri dari P0= 85% rumput kumpai tembaga + 15% konsenterat, P1= 55% rumput kumpai tembaga + 30% daun singkong+15% konsenterat + 0,05% (Saccharomycas Cerevisiae), P2= 55% rumput kumpai tembaga+30% lumpur sawit+15% konsenterat+0,05% (Saccharomycas Cerevisiae), P3= 55% rumput kumpai tembaga+15% lumpur sawit+15% daun singkong+15% konsenterat + 0,05 % (Saccharomycas Cerevisiae). Parameter yang diamati adalah konsentrasi SCFA (mM), Gas Metana (ppm), dan laju produksi Gas (mL/Jam) secara in vitro. Hasil penelitian menunjukkan bahwa ransum rumput kumpai tembaga dikombinasi daun singkong, lumpur sawit dan disuplementasi ragi Sc menghasilkan Konsentrasi SCFA, Gas metana, dan Produksi Gas yang berbeda nyata (P<0,05) secara in vitro. Berdasarkan hasil penelitian dapat disimpulkan bahwa perlakuan R2 (30% lumpur sawit dan 0,05% ragi) mampu mencukupi sumber energi utama ternak dengan konsentrasi SCFA yaitu 115,25 mM, dan memiliki nilai konsentrasi gas metana yang lebih rendah yaitu 537,97 ppm, serta dapat meningkatkan nilai laju produksi gas yaitu 1.44 mL/Jam. Kata Kunci : Kombinasi, Kumpai Tembaga, Metana, Produksi Gas, SCFA. ABSTRACTQuality grass (Hymenachne Acutigluma) is a forage that abundant production but has not been optimally utilized as animal feed. Therefore, it needs to be combined Cassava Leaves, Palm Oil and yeast Sc Suplementation to improve the nutrients of the copper based grass ration. This study aims to determine the concentration of SCFA and in vitro gas production of grass based grass ration based on cassava leaves, palm oil and Yeast Sc supplementation. This study used a complate random design method (CRD) that 4 treatments and 4 replications. The treatment consisted of P0 = 85% (Hymenachne Acutigluma) + 15% consentate, P1 = 55% (Hymenachne Acutigluma) + 30% cassava leaves + 15% consentate + 0.05% (Saccharomycas Cerevisiae), P2 = 55% (Hymenachne Acutigluma) + 30% palm oil + 15% consentate + 0.05% (Saccharomycas Cerevisiae), P3 = 55% of copper grass + 15% palm oil + 15% cassava leaves + 15% consentate + 0,05% (Saccharomycas Cerevisiae). Parameters observed were SCFA concentration (mM), Methane Gas (ppm), and Gas production (mL / Hour) in vitro. The results showed that raccoon grass (Hymenachne Acutigluma) combined cassava leaves, palm oil and yeast Sc suplementation resulted in a significantly different concentration of SCFA, methane gas and gas production (P <0.05) in vitro. Based on research result, it can be concluded that R2 treatment (30% palm oil and 0.05% yeast Sc) is able to supply primary energy source of livestock with SCFA concentration of 115,25 mM, and have lower methane gas concentration value that is 537,97 ppm, and can increase the gas production rate of 1.44 mL / hour Keywords: Combination, Gas Production, Hymenachne Acutigluma, Methane, SCFA.


2016 ◽  
Vol 9 (2) ◽  
pp. 94
Author(s):  
Sri Rahayu ◽  
Handojo Hadi Nurjanto ◽  
Rahman Gilang Pratama

Acacia decurrens merupakan salah satu jenis tanaman yang tumbuh mendominasi kawasan Taman Nasional Gunung Merapi (TNGM), pasca erupsi Gunung Merapi tahun 2010. Sekitar 80% tegakan A. decurrens di kawasan tersebut menunjukkan gejala busuk batang akibat infeksi jamur Ceratocystis sp. yang umumnya dipicu oleh luka gerekan kumbang dari kelompok ambrosia. Penelitian bertujuan untuk : (1) mendeskripsikan karakter morfologi jamur Ceratocystis sp., serta kemampuannya beradaptasi pada beberapa jenis tanaman hutan, (2) mengevaluasi status penyakit busuk batang oleh jamur Ceratocystis sp. Karakter morfologi dan kemampuan adaptasinya pada inang akasia, melina, jabon, sengon, dan jati dilakukan di Laboratorium Perlindungan dan Kesehatan Hutan, Fakultas Kehutanan UGM. Survei untuk evaluasi status penyakit busuk batang dilakukan pada bulan Februari sampai Agustus 2014 di demplot restorasi pasca erupsi Merapi (luas 8,4 ha), dengan intensitas sampling 8%. Berdasarkan karakter morfologi, terdapat 2 isolat jamur Ceratocystis sp. yaitu asal lembah (L) dan dari bukit (B) dengan warna koloni krem, luas koloni 20-22 cm2 pada umur 14 hari, membentuk konidia menyerupai tong, dan silindris. Sifat lainnya yaitu memiliki kemampuan yang sama untuk tumbuh, mengkolonisasi, dan menginfeksi inang akasia, sengon, jabon, dan melina, tetapi tidak mampu tumbuh pada inang jati. Berdasarkan luas serangan, status penyakit busuk batang berkisar antara sangat umum sampai menyebar luas (luas serangan = 54-100%), dengan tingkat keparahan bekisar antara ringan sampai parah (intensitas penyakit = 15-67%).Kata kunci: Ceratocystis sp., Acacia decurrens, luas serangan, intensitas penyakit, Taman Nasional Gunung Merapi. Characteristic of stem rot diseases caused by Ceratocystis sp. on Acacia decurrens and its status in Gunung Merapi National Park, YogyakartaAbstractMount Merapi National Park (TNGM) has been dominated by Acacia decurrens after the eruption in 2010. Almost 80% of A. decurrens trees showed stem rot diseases caused by Ceratocystis sp. which may associate with stem wound induced by ambrosia beetle and other physical injuries. The research objective were (1) to characterize the morphological feature, in vitro growth, and ability to adapt, colonize as well as to infect akasia, jabon, sengon, melina and jati sedlings, (2) to evaluate the status of stem rot disease in TNGM demonstration plot. Laboratory work was conducted in order to study the morphological feature of the fungus, in vitro growth on PDA media, and to evaluate its compatibility to growth, colonize, and infect on 5th month seedling of akasia, sengon, jati, jabon and melina. Field monitorings were conducted from February to August 2014 at the restoration plot (8.4 ha) at 8% sampling intensity. Disease status was evaluated in order to ascertain the disease incidence and severity of stem rot disease at the demonstration plot area. Two Ceratocistys isolates found from the hill (B) and valley (L) had similar characteristics on morphologic features i.e. cream color, 20 to 22 cm2 colony size at 14 days growth in PDA media, having both cylindrical and barrel shaped conidia. The other characteristics of the Ceratocistys were an ability to growth, to colonize, and to infect akasia, sengon, melina and jabon, except on jati. The status of stem rot disease was ranged from highly common to widespread (disease incidence = 54%-100%) as the disease severity status was ranged from low to severe (disease severity = 15%-67%).


2020 ◽  
Vol 27 (29) ◽  
pp. 4778-4788 ◽  
Author(s):  
Victoria Heredia-Soto ◽  
Andrés Redondo ◽  
José Juan Pozo Kreilinger ◽  
Virginia Martínez-Marín ◽  
Alberto Berjón ◽  
...  

Sarcomas are tumours of mesenchymal origin, which can arise in bone or soft tissues. They are rare but frequently quite aggressive and with a poor outcome. New approaches are needed to characterise these tumours and their resistance mechanisms to current therapies, responsible for tumour recurrence and treatment failure. This review is focused on the potential of three-dimensional (3D) in vitro models, including multicellular tumour spheroids (MCTS) and organoids, and the latest data about their utility for the study on important properties for tumour development. The use of spheroids as a particularly valuable alternative for compound high throughput screening (HTS) in different areas of cancer biology is also discussed, which enables the identification of new therapeutic opportunities in commonly resistant tumours.


2020 ◽  
Vol 7 (03) ◽  
Author(s):  
PREM PANDEY ◽  
G. C. SAGAR ◽  
SUNDARMAN SHRESTHA2 ◽  
HIRAKAJI MANANDHAR ◽  
RITESH K. YADAV ◽  
...  

Nine isolates of Trichoderma spp. were isolated from different agro- ecological regions of Nepal viz; Jumla, Palpa, Chitwan, Tarahara, Banke, Illam and Salyan and screened against Sclerotium rolfsii Sacc. Adreded soil borne phytopathogen causing collar rot of chickpea in chickpea; In-vitro efficacy of nine fungal antagonist (Trichoderma spp.) against Sclerotium rolfsii were screened. Pot experiment was done to find out the effective management of S. rolfsi through Tricoderma using different methods i.e. Seed treatment, soil drenching and soil application. All the tested isolates of Trichoderma spp. were found effective on mycelial growth inhibition and sclerotial parasitization of S. rolfsii. Trichoderma isolated from Palpa district showed maximum growth inhibition (%) of pathogen periodically after 48(93.78%), 72(96.00%), 96(97.96%) and 120(100.00%) hours of inoculation. Parasitized sclerotium showed minimum sclerotial germination on agar plates. Moreover, Trichoderma species isolated from Palpa districts showed second best percent mycelial growth inhibition periodically at 72(25.00%), 120(29.16%), 168(29.16%) and 216(29.16%).In pot experiment at 40 days after sowing, Seedling height was maximum in soil drenching with 30g per 100ml of water (22.27cm) and Mortality percentage of seedlings was least or highest disease control was observed in seed treated with 109cfu/ml (0.000%).


2020 ◽  
Vol 7 (Supplement_1) ◽  
pp. S725-S725
Author(s):  
Mariana Castanheira ◽  
Timothy B Doyle ◽  
Cory Hubler ◽  
Rodrigo E Mendes ◽  
Helio S Sader

Abstract Background Most CRE isolates in US hospitals produce KPC enzymes, but some do not carry carbapenemases. We investigated the prevalence, resistance mechanisms and activity of ceftazidime-avibactam and comparator agents against CRE that did not carry carbapenemase genes from US hospitals. Additionally, meropenem-resistant isolates were tested for meropenem-vaborbactam. Methods A total of 28,904 Enterobacterales isolates were collected in 70 US hospitals during 2016-2018, and susceptibility tested by reference broth microdilution. Meropenem-vaborbactam was tested using lyophilized panels following the manufacturer’s instructions. CRE isolates were submitted to whole genome sequencing for the screening of b-lactamase genes, multilocus sequence typing, changes in outer membrane protein (OMP) genes and AmpC expression levels. Results A total of 304 (1.1%) CREs were observed in the study period and 45 (14.8%) isolates did not carry carbapenemases. These isolates were mainly Klebsiella aerogenes, Enterobacter cloacae and Klebsiella pneumoniae (11, 11 and 10 isolates, respectively), but also included 5 other species. Acquired b-lactamase genes were detected among 17 isolates and blaCTX-M-15 was the most common (13 isolates). All K. aerogenes and 10 E. cloacae did not carry acquired b-lactamase genes. Ceftazidime-avibactam (100% susceptible) inhibited all isolates at the current breakpoint, followed by tigecycline and amikacin (&gt; 80% susceptible). Other comparators were not active against non-carbapenemase-producing CRE. Nine of 35 meropenem-resistant isolates displayed meropenem-vaborbactam MIC values of ≥ 8 mg/L (nonsusceptible). Further analysis showed that 23 isolates had disruption of OmpC/OmpK36, 4 had disrupted OmpF/OmpK35 and 13 had both OMP genes disrupted. Additionally, 7 isolates had elevated AmpC expression among 17 isolates tested. Among 7 E. coli, 4 were ST131 and only 2 of 10 K. pneumoniae were clonal complex 11. Conclusion Therapy options for treatment of infections caused by CRE were very limited until recent approval of new agents with activity against these isolates. Ceftazidime-avibactam demonstrated full in vitro activity against all carbapenemase-negative CRE carrying multiple resistance mechanisms. Disclosures Mariana Castanheira, PhD, 1928 Diagnostics (Research Grant or Support)A. Menarini Industrie Farmaceutiche Riunite S.R.L. (Research Grant or Support)Allergan (Research Grant or Support)Allergan (Research Grant or Support)Amplyx Pharmaceuticals (Research Grant or Support)Cidara Therapeutics (Research Grant or Support)Cidara Therapeutics (Research Grant or Support)Cipla Ltd. (Research Grant or Support)Cipla Ltd. (Research Grant or Support)Fox Chase Chemical Diversity Center (Research Grant or Support)GlaxoSmithKline (Research Grant or Support)Melinta Therapeutics, Inc. (Research Grant or Support)Melinta Therapeutics, Inc. (Research Grant or Support)Melinta Therapeutics, Inc. (Research Grant or Support)Merck (Research Grant or Support)Merck (Research Grant or Support)Merck & Co, Inc. (Research Grant or Support)Merck & Co, Inc. (Research Grant or Support)Paratek Pharma, LLC (Research Grant or Support)Pfizer (Research Grant or Support)Qpex Biopharma (Research Grant or Support) Timothy B. Doyle, Allergan (Research Grant or Support)Allergan (Research Grant or Support)Cipla Ltd. (Research Grant or Support)Melinta Therapeutics, Inc. (Research Grant or Support)Pfizer (Research Grant or Support)Qpex Biopharma (Research Grant or Support) Cory Hubler, Allergan (Research Grant or Support) Rodrigo E. Mendes, PhD, A. Menarini Industrie Farmaceutiche Riunite S.R.L. (Research Grant or Support)Allergan (Research Grant or Support)Allergan (Research Grant or Support)Basilea Pharmaceutica International, Ltd (Research Grant or Support)Cipla Ltd. (Research Grant or Support)Department of Health and Human Services (Research Grant or Support)GlaxoSmithKline (Research Grant or Support)Melinta Therapeutics, Inc. (Research Grant or Support)Merck (Research Grant or Support)Merck (Research Grant or Support)Pfizer (Research Grant or Support) Helio S. Sader, MD, PhD, A. Menarini Industrie Farmaceutiche Riunite S.R.L. (Research Grant or Support)Allergan (Research Grant or Support)Allergan (Research Grant or Support)Allergan (Research Grant or Support)Cipla Ltd. (Research Grant or Support)Cipla Ltd. (Research Grant or Support)Melinta (Research Grant or Support)Merck (Research Grant or Support)Merck (Research Grant or Support)Paratek Pharma, LLC (Research Grant or Support)Pfizer (Research Grant or Support)


2021 ◽  
Vol 12 (1) ◽  
Author(s):  
David S. Fischer ◽  
Meshal Ansari ◽  
Karolin I. Wagner ◽  
Sebastian Jarosch ◽  
Yiqi Huang ◽  
...  

AbstractThe in vivo phenotypic profile of T cells reactive to severe acute respiratory syndrome (SARS)-CoV-2 antigens remains poorly understood. Conventional methods to detect antigen-reactive T cells require in vitro antigenic re-stimulation or highly individualized peptide-human leukocyte antigen (pHLA) multimers. Here, we use single-cell RNA sequencing to identify and profile SARS-CoV-2-reactive T cells from Coronavirus Disease 2019 (COVID-19) patients. To do so, we induce transcriptional shifts by antigenic stimulation in vitro and take advantage of natural T cell receptor (TCR) sequences of clonally expanded T cells as barcodes for ‘reverse phenotyping’. This allows identification of SARS-CoV-2-reactive TCRs and reveals phenotypic effects introduced by antigen-specific stimulation. We characterize transcriptional signatures of currently and previously activated SARS-CoV-2-reactive T cells, and show correspondence with phenotypes of T cells from the respiratory tract of patients with severe disease in the presence or absence of virus in independent cohorts. Reverse phenotyping is a powerful tool to provide an integrated insight into cellular states of SARS-CoV-2-reactive T cells across tissues and activation states.


2020 ◽  
Vol 7 (Supplement_1) ◽  
pp. S655-S655
Author(s):  
Daniel Navas ◽  
Angela Charles ◽  
Amy Carr ◽  
Jose Alexander

Abstract Background The activity of imipenem/relebactam (I/R), ceftazidime/avibactam (CZA) and cefiderocol (FDC) were evaluated against clinical isolates of multidrug resistant (MDR) strains of P. aeruginosa which was resistant to ceftolozane/tazobactam (C/T). The recent increase of MDR P. aeruginosa strains isolated from clinical samples has prompted research and development of new antimicrobials that can withstand its multiple resistance mechanisms. C/T is an effective option for treatment of MDR P. aeruginosa in our facility with only 10% of resistance in MDR strains, but the emergence of resistance may occur due to the presence of a carbapenemase gene or an ampC mutation. Methods Antimicrobial susceptibility testing for C/T Etest® (bioMérieux, Inc.) were performed on all MDR strains initially screened by the VITEK2® (bioMérieux, Inc.). 10% (n=20) of all MDR isolates were resistant to C/T by the CLSI 2019 breakpoints. These resistant isolates were tested for presence of a carbapenemase gene using the GeneXpert CARBA-R (Cepheid®) PCR and against CZA Etest® (bioMérieux, Inc.) I/R gradient strips (Liofilchem®) and FDC broth microdilution (Thermo Scientific™ Sensititre™). Results A total of 20 clinical isolates of MDR P. aeruginosa resistant to C/T were tested following standardized CLSI protocols and techniques. All 20 isolates were screened for the presence of a carbapenemase gene (blaVIM, blaNDM, blaKPC, blaOXA-48, blaIMP). A blaVIM gene was detected in 6 (30%) out of 20 isolates. FDC demonstrated the greatest activity with 85% (n=17) of susceptible isolates (CLSI MIC &lt;4µg/dL). CZA (CLSI MIC &lt;8µg/dL) and I/R (FDA MIC &lt;2µg/dL) showed 15% (n=3) and 10% (n=2) of susceptible isolates respectively. FDC was active against all 6 blaVIM isolates, where all 6 strains were resistant to CZA and I/R as expected. 3 isolates tested non-susceptible against FDC; additional characterization was not performed at this time. Conclusion Based on these results, FDC demonstrated the greatest in-vitro activity against C/T resistant strains of MDR P. aeruginosa. FDC also demonstrated activity against all 6 MDR P. aeruginosa carrying blaVIM gene. FDC is a strong option to consider on MDR P. aeruginosa strains based on a resistance testing algorithm and a cost/effective protocol. Disclosures All Authors: No reported disclosures


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