PERTUMBUHAN DAN MORFOLOGI KULTUR TUNAS SEMPUR (Dillenia philippinensis Rolfe) PADA MEDIA MS-BAP-NAA (Growth and Morphology of Sempur (Dillenia philippinensis Rolfe) Shoot Culture on MS –BAP-NAA Media)

Abstract The aim of the study was to develop an efficient micropropagation system for Rosa ‘Konstancin’, an interspecific hybrid between R. rugosa and R. beggeriana, whose fruits have high pro-health value. Shoot cultures were initiated from shoot buds collected in May and August from 15-year-old field-grown Rosa ‘Konstancin’ shrubs. The effect and interaction of different concentrations of phytohormones, sucrose and iron sources on in vitro initiation, multiplication and rooting of shoots were studied. The time of collecting explants from donor plants significantly affected the initiation of shoot culture of Rosa ‘Konstancin’. Considerably higher frequency of bud break (100%) was obtained in explants isolated in August as compared to those collected at the end of May (30%). All buds developed into single shoots after 2-4 weeks of growing on the basal Murashige and Skoog medium containing 2.2 µM BAP, 0.3 µM GA3 and 88 mM of sucrose. The highest multiplication rate (4.8 shoots/explant) in a 5-week period was obtained on MS medium containing 50% of nitrogen salts, 3.1 µM BAP, 0.9 µM GA3 and 58 mM sucrose. High rooting frequency (100%) and quality of rooted plantlets was obtained on a medium containing 0.5 µM IBA, 138 µM Fe-EDDHA and 88 mM sucrose. Fe-EDDHA had a beneficial effect on the growth and photosynthetic activity of Rosa ‘Konstancin’ plantlets, which were successfully acclimatized ex vitro, with a more than 90% survival rate.

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In vitro shoot culture of Rhododendron fortunei: An important plant for bioactive phytochemicals

Industrial Crops and Products ◽

10.1016/j.indcrop.2018.10.037 ◽

2018 ◽

Vol 126 ◽

pp. 459-465 ◽

Cited By ~ 5

Author(s):

Xiangying Wei ◽

Jianjun Chen ◽

Chunying Zhang ◽

Zonghua Wang

Keyword(s):

Shoot Culture ◽

In Vitro Shoot Culture ◽

Bioactive Phytochemicals

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Establishment of a Rapid Plant Regeneration System in Physalis angulata L. through Axillary Meristems

Notulae Scientia Biologicae ◽

10.15835/nsb749707 ◽

2015 ◽

Vol 7 (4) ◽

pp. 471-474

Author(s):

Owk ANIEL KUMAR ◽

Songa RAMESH ◽

Sape SUBBA TATA

Keyword(s):

Shoot Culture ◽

Shoot Multiplication ◽

Multiple Shoot ◽

Herbaceous Plant ◽

Axillary Meristem ◽

Regeneration System ◽

Axillary Meristems ◽

Meristem Explants ◽

Physalis Angulata

An optimal plant propagation method of Physalis angulata L., a medicinally important herbaceous plant species has been developed using axillary meristem explants. Shoot bud proliferation was initiated from axillary meristem explants cultured on MS medium supplemented with various concentrations of 0.5-2.5mg/L/(BAP)/(Zeatin)/(KIN). The maximum in vitro response of shooting frequency of explants (88.1%) and shoots per explant (42) was achieved with medium containing 1.0mg/L BAP. Multiple shoot culture was established by repeated subculturing of the shoot buds of axillary meristems on shoot multiplication medium. Among the subculture media BAP in combination with 1.5mg/L (IAA)+0.25mg/L(GA3) produced maximum shoots per explant (128±0.29) after two weeks of culture. Effective in vitro shoot elongation and rooting was achieved on 1.0mg/L(GA3) and 1.0mg/L(IBA), respectively. Most of the generated shoots were successfully transferred to soil under field conditions. The survival percentage of the transferred plants on soil was found to be 90 per cent. This protocol can be used for commercial propagation and for future genetic improvement studies.

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Improved culture ability of Shoot Culture Derived Protoplasts of Solanum Tuberosum L. by Use of activated charcoal

Protoplasts 1983 ◽

10.1007/978-3-0348-6556-2_113 ◽

1983 ◽

pp. 258-259

Author(s):

I. Carlberg ◽

K. Glimelius ◽

T. Eriksson

Keyword(s):

Solanum Tuberosum ◽

Activated Charcoal ◽

Solanum Tuberosum L ◽

Shoot Culture

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In vitro arrow cane (Gynerium sagitatum Aubl.) multiplication in double phase medium

Revista de Ciencias Agrícolas ◽

10.22267/rcia.183502.86 ◽

2018 ◽

Vol 35 (2) ◽

pp. 5-13

Author(s):

Claudia Marcela Lopez D. ◽

Isidro Elias Suarez P.

Keyword(s):

Plant Material ◽

Shoot Culture ◽

Indigenous Communities ◽

Fresh Medium ◽

Multiplication Rate ◽

Semisolid Medium ◽

Double Phase ◽

Medium System ◽

Phase Medium ◽

Micropropagated Plants

Arrow cane (Gynerium sagitatum Aubl.) is a Poaceae species used as fiber source to make traditional and valuable handmade craftsmanship by indigenous communities in Northern Colombia. Since no commercial crops are established fiber needs are taken from natural plant populations affecting ecosystem. A micropropagation protocol to clonally multiply large quantities of arrow cane plant material for planting commercial crops has been developed; however, micropropagated plants are costly compared to naturally extracted plant material. To reduce micropropagated plants costs, in the present research a double phase medium formulation along with continuous shoot culture with no periodic transfers to fresh medium was compared to semisolid medium system with subculture every four weeks with respect to multiplication rate and costs of micropropagated plants. The results showed that continuous culture of explants with double phase medium and no periodic transfers resulted in higher multiplication rates and larger shoots compared to shoots cultured using the conventionalsemisolid medium system and transfer to fresh medium every four weeks. Plants from both, semisolid and double phase culture system, fully adapted and recovered when transferred to ex vitro conditions. The cost analysis showed that double phase cultured shoots are ≥20% less expensive.

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Antioxidants and Phenolic Secretion in Sugarcane Genotypes Shoots Culture

Journal of Agricultural Science ◽

10.5539/jas.v10n5p79 ◽

2018 ◽

Vol 10 (5) ◽

pp. 79

Author(s):

Ana Da Silva Ledo ◽

Maria M. Jenderek ◽

Carlos Alberto Da Silva Ledo ◽

Tomas Ayala-Silva

Keyword(s):

Principal Component Analysis ◽

Citric Acid ◽

Phenolic Compounds ◽

Shoot Culture ◽

Shoot Proliferation ◽

Principal Component ◽

Shoot Formation ◽

Regenerative Capacity ◽

In Vitro Shoot Culture

Secretion of phenolic compounds is a major limitation for sugarcane in vitro shoot culture, causing a loss of regenerative capacity and subsequent cell death. In this study, micropropagation and phenolic secretion of four Saccharum genotypes were evaluated in presence of different antioxidants. Aseptic cultures of S. officinarum (PI 184794 and PI 88652), S. sinense (PI 29109) and S. robustum (UNK R65P35) were propagated on medium containing antioxidants, citric acid (100 mg/L), L-cysteine (100 mg/L), polyvynylpirrolidone (300 mg/L) and L-glutathione (50 mg/L) in two consecutive subculture cycles. Interaction between genotypes and antioxidants was significant in both cycles. All genotypes showed good shoot formation, shoot vigor and color, except in PI 88652 which had less shoot development in both the presence and absence of the antioxidants tested. PI 184794 displayed the highest shoot proliferation in the presence of citric acid, and UNK R65P35 produced more shoots per explant in the 2nd subculture. For S. sinense (PI 29109), in both subcultures, most shoots were observed in the presence of polyvynylpirrolidone. Medium discoloration due to phenolic secretion was reduced in the presence of citric acid and polyvynylpirrolidone. The type of secreted phenolic compounds differed with genotype as the Principal Component Analysis of cultivation media separated PI 88652 from PI 29109 and UKN R65P35. Phenolic compounds varied in composition and were secreted at various levels as a function of genotype and antioxidant type. Loadings plots indicated the genotype and antioxidant separations were broadly driven by flavonoid compounds.

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The Stimulatory Effect of Purine-Type Cytokinins on Proliferation and Polyphenolic Compound Accumulation in Shoot Culture of Salvia viridis

Biomolecules ◽

10.3390/biom10020178 ◽

2020 ◽

Vol 10 (2) ◽

pp. 178 ◽

Cited By ~ 2

Author(s):

Izabela Grzegorczyk-Karolak ◽

Katarzyna Hnatuszko-Konka ◽

Mariola Zarzycka ◽

Łukasz Kuźma

Keyword(s):

Therapeutic Potential ◽

Shoot Culture ◽

Total Content ◽

Biomass Accumulation ◽

Hormonal Control ◽

Polyphenolic Compound ◽

Physiological Processes ◽

Best Response ◽

Alternative Sources ◽

Metabolite Content

The present study demonstrates hormonal control of Salvia viridis growth and development using four different purine-type cytokinins at different concentrations. The addition of cytokinins significantly increased biomass of cultures, proliferation rate, and, interestingly, secondary metabolite production. The best response in terms of multiplication ratio was recorded on Murashige and Skoog medium supplemented with 0.5 mg/L BPA (N-benzylotetrahydropyranyl adenine), while the greatest biomass accumulation was achieved when supplemented with 1 mg/L m-T (meta-topoline). Quantitative UPLC-DAD analysis of the hydromethanolic extract from S. viridis culture revealed the presence of 12 polyphenols: seven phenolic acids and five phenylethanoids. The highest total content of polyphenolic compounds was found in shoots cultivated on medium with 2 mg/L BPA (18.66 mg/g DW): almost twice that of control shoots. The medium was also the most optimal for the biosynthesis of rosmarinic acid, the predominant phenolic acid. However, the greater phenylethanoid accumulation was stimulated by 1 mg/L m-T: the metabolite content was above three times higher than that found in shoots grown on the control medium (8.03 mg/g DW vs. 2.37 mg/g DW). Hence, it was demonstrated that phytohormones are capable of influencing not only vital physiological processes, but therapeutic potential of plants as well. Therefore, the cytokinin-based sage cultures may be also considered as the alternative sources of bioactive compounds.

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In vitro and whole-plant magnitude and cross-resistance characterization of two imidazolinone-resistant sugarbeet (Beta vulgaris) somatic cell selections

Weed Science ◽

10.1017/s0043174500090123 ◽

1998 ◽

Vol 46 (1) ◽

pp. 24-29 ◽

Cited By ~ 8

Author(s):

Terry R. Wright ◽

Donald Penner

Keyword(s):

Somatic Cell ◽

Shoot Culture ◽

Acetolactate Synthase ◽

Cross Resistance ◽

Shoot Cultures ◽

Als Inhibitors ◽

Whole Plants ◽

Inhibitor Resistance ◽

Als Inhibitor

Acetolactate synthase (ALS)-inhibiting herbicide carryover in soil can severely affect sugarbeets grown in the year(s) following application. Two newly developed imidazolinone-resistant (IMI-R) sugarbeet somatic cell selections (Sir-13 and 93R30B) were examined for magnitude of resistance and extent of cross-resistance to other classes of ALS inhibitors and compared to a previously developed sulfonylurea-resistant (SU-R) selection, Sur. In vitro shoot culture tests indicated Sir-13 resistance was specific to imidazolinone (IMI) herbicides at approximately a 100-fold resistance compared to the sensitive control sugarbeet. Sur was 10,000-fold resistant to the sulfonylurea (SU) herbicide, chlorsulfuron, and 40-fold resistant to the triazolopyrimidine sulfonanilide (TP) herbicide, flumetsulam, but not cross-resistant to the IMI herbicides. 93R30B was selected for IMI-R from a plant homozygous for the SU-R allele,Sur, and displayed similar in vitro SU-R and TP-R as Sur, but also displayed a very high resistance to various IMI herbicides (400- to 3,600-fold). Compared to the sensitive control, Sir-13 was 300- and > 250-fold more resistant to imazethapyr and imazamox residues in soil, respectively. Response by whole plants to postemergence herbicide applications was similar to that observed in shoot cultures. Sir-13 exhibited > 100-fold resistance to imazethapyr as well as imazamox, and 93R30B showed > 250-fold resistance to both herbicides. 93R30B showed great enough resistance to imazamox to merit consideration of imazamox for use as a herbicide in these sugarbeets. Sir-13 showed a two- to threefold higher level of resistance in the homozygous vs. heterozygous state, indicating that like most ALS-inhibitor resistance traits, it was semidominantly inherited.

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