Black cumin Potential Anticancer effect of Black Cumin seed
(Nigella sativa L.) extracts as determined by Cytotoxicity test
against Larvae of Artemia salina Leach using Brine Shrimp
Lethality Test (BSLT)

Black cumin plant seed or black seed (Nigella sativa L.) is among the most commonly used spices. This plant is grown mostly for its spicy seeds. Studies have reported that black cumin seeds have high levels of antioxidant that correlates with anticancer activities. The current study aims to determine the cytotoxic effect of black seed extracts on brine shrimp larvae (Artemia salina Leach) using Brine Shrimp Lethality Test (BSLT). Black cumin seed extract was obtained through percolation method using an extraction solvent (ethanol 96%). The cytotoxicity test was performed at 0 ppm concentration (control) and 10, 50, 100, 250 and 500 ppm concentrations of black seed extracts. We use 10 Brine Shrimp larvae per tube and repeated the test three times for each test group. Our results show that ethanolic extract (90%) of black cumin seeds (Nigella sativa L.) has a strong cytotoxic effect on Artemia salina larvae with the LC50 value of 107.2 ppm.

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Uji Potensi Daya Hambat Ekstrak Metanol Biji Jintan Hitam (Nigella Sativa L.) Terhadap Bakteri Streptococcus

Jurnal Surya Medika ◽

10.33084/jsm.v6i1.1615 ◽

2020 ◽

Vol 6 (1) ◽

pp. 18-21

Author(s):

Guntur Satrio Pratomo ◽

Nurul Chusna ◽

Muhammad Priyadi

Keyword(s):

Seed Oil ◽

Methanol Extract ◽

Nigella Sativa ◽

High Concentration ◽

Black Cumin ◽

Black Seed ◽

Anti Cancer ◽

Black Cumin Seed ◽

Streptococcus Mutants ◽

Cumin Seed

One of the plants that are often used as medicinal plants is Black Seed, Black Seed is a medicinal plant known in Indonesia by the name of black cumin. Black cumin seeds and essential oils (Nigella sativa L.) have been widely used in traditional medicine. Many studies have proven the effects of Nigella sativa extract. In various studies, black cumin seed oil shows efficacy as an anti-cancer, anti-free radical and immunomodulator, analgesic, antimicrobial, anti-inflammatory, a spasmolytic, bronchodilator, hepatoprotective, and anti-hypertensive Advanced chemical analysis found that Black Seed contains carotene that is converted into vitamin A by heart. The purpose of this study was to determine the potential inhibition of methanol extract of black cumin seeds (Nigella Sativa L.) against Streptococcus. This research uses a quantitative approach with an experimental method. This research was conducted in the Pharmacognition and Microbiology Laboratory of the Faculty of Health Sciences, Muhammadiyah University, Palangkaraya. The sample used was a sample of plant material, namely Black Cumin Seed (Nigella sativa L) which had been dried and pollinated and then extracted and then tested against bacteria. The results of testing the antibacterial activity showed that from low concentration to high concentration the diameter of the resulting inhibition showed that the activity could inhibit the bacteria streptococcus mutants. The highest inhibition at a concentration of 6% is 0.83 mm and the lowest concentration at a concentration of 3% against Streptococcus mutants.

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EFFECTIVENESS TEST OF BLACK CUMIN SEEDS (NIGELLA SATIVA) EXTRACT ON THE GROWTH OF KLEBSIELLA PNEUMONIAE AND PSEUDOMONAS AERUGINOSA BACTERIA

BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) ◽

10.31289/biolink.v7i2.3702 ◽

2021 ◽

Vol 7 (2) ◽

pp. 130-138

Author(s):

Nadya Nazimuddin Putri ◽

Linda Chiuman ◽

Chrismis Novalinda Ginting ◽

Ermi Girsang

Keyword(s):

Pseudomonas Aeruginosa ◽

Klebsiella Pneumoniae ◽

Nigella Sativa ◽

Ethanol Extract ◽

Inhibition Zone ◽

Seed Extract ◽

Diffusion Method ◽

Black Cumin ◽

Black Cumin Seed ◽

Cumin Seed

Black cumin seeds are known to have beneficial contents as antibacterial. This study aimed to determine the effectiveness of black cumin seed extract (Nigella sativa) on the growth of Klebsiella pneumoniae and Pseudomonas aeruginosa. The method used was the disc diffusion method. The ethanol extract of black cumin seeds gain by maceration method and was made into various concentration (5, 7.5, 10, 12.5, and 15)%. For positive control, ciprofloxacin disc were used and negative control used distilled water. The research model used was in vitro experimental research. The results showed that ethanol extract of black cumin seeds have antibacterial activity against Klebsiella pneumoniae with inhibition zone diameters (4.5, 6.7, 10.1, 11, 12.8) mm, Pseudomonas aeruginosa with inhibition zone diameters (7.5, 12, 12.6, 13.4, 15.6) mm, positive controls respectively (24.3 and 20.7) mm and negative controls (0). It can be concluded that black cumin seed extract has an inhibiting effect on the growth of Klebsiella pneumoniae and Pseudomonas aeruginosa.

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Effects of black cumin seed (Nigella sativa L.), a probiotic, a prebiotic and a synbiotic on growth performance, immune response and blood characteristics of male broilers

Livestock Science ◽

10.1016/j.livsci.2014.03.014 ◽

2014 ◽

Vol 164 ◽

pp. 128-134 ◽

Cited By ~ 37

Author(s):

Hossein A. Ghasemi ◽

Niloofar Kasani ◽

Kamran Taherpour

Keyword(s):

Immune Response ◽

Growth Performance ◽

Nigella Sativa ◽

Black Cumin ◽

Blood Characteristics ◽

Black Cumin Seed ◽

Cumin Seed

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BLACK CUMIN SEED (NIGELLA SATIVA LINN.) OIL AND ITS FRACTIONS PROTECT AGAINST BETA AMYLOID PEPTIDE-INDUCED TOXICITY IN PRIMARY CEREBELLAR GRANULE NEURONS

Journal of Food Lipids ◽

10.1111/j.1745-4522.2008.00137.x ◽

2008 ◽

Vol 15 (4) ◽

pp. 519-533 ◽

Cited By ~ 12

Author(s):

NORSHARINA ISMAIL ◽

MAZNAH ISMAIL ◽

LATIFFAH A. LATIFF ◽

MUSALMAH MAZLAN ◽

ABDALBASIT A. MARIOD

Keyword(s):

Nigella Sativa ◽

Beta Amyloid ◽

Amyloid Peptide ◽

Cerebellar Granule Neurons ◽

Granule Neurons ◽

Cerebellar Granule ◽

Black Cumin ◽

Beta Amyloid Peptide ◽

Black Cumin Seed ◽

Cumin Seed

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Ethanol Extract of Black Cumin Seed (Nigella Sativa) Reduces Expression Kidney iNOS in Mice Model Preeclampsia

Medical Laboratory Technology Journal ◽

10.31964/mltj.v0i0.247 ◽

2019 ◽

Vol 5 (2) ◽

pp. 88

Author(s):

Rubiati Hipni

Keyword(s):

Nigella Sativa ◽

Ethanol Extract ◽

Negative Control ◽

Inos Expression ◽

Control Group ◽

Mice Model ◽

Control Group Design ◽

Black Cumin ◽

Black Cumin Seed ◽

Cumin Seed

This study aims to analyze the effect of ethanol extract of black cumin seeds (Nigella Sativa) on iNOS expression in the kidney of mice model preeclampsia. Design This study uses an experimental design with a posttest only control group design. Using a mice model preeclampsia. This study was divided into 6 groups: negative control, positive control (preeclampsia model), model + black cumin seed extract 500 mg/KgBB/day, 1000 mg/KgBB/day, 1500 mg/KgBB/day, and 2000 mg/KgBB/day days, after surgery on mice then examined iNOS Kidney expression by methods Immunohistochemical. Data from observations were analyzed by one way ANOVA test. The result there is an effect of ethanol extract of black cumin seeds on renal iNOS expression in mice model preeclampsia (p <0.05) at doses of 500mg, 1000mg, 1500mg, and 2000mg on decreasing renal iNOS expression. The conclusion Ethanol extract of black cumin seeds (Nigella sativa) can reduce iNOS Kidney expression in Mice Preeclampsia.

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