scholarly journals EFFECTIVENESS TEST OF BLACK CUMIN SEEDS (NIGELLA SATIVA) EXTRACT ON THE GROWTH OF KLEBSIELLA PNEUMONIAE AND PSEUDOMONAS AERUGINOSA BACTERIA

2021 ◽  
Vol 7 (2) ◽  
pp. 130-138
Author(s):  
Nadya Nazimuddin Putri ◽  
Linda Chiuman ◽  
Chrismis Novalinda Ginting ◽  
Ermi Girsang
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Klebsiella Pneumoniae ◽  
Nigella Sativa ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Seed Extract ◽  
Diffusion Method ◽  
Black Cumin ◽  
Black Cumin Seed ◽  
Cumin Seed

Black cumin seeds are known to have beneficial contents as antibacterial. This study aimed to determine the effectiveness of black cumin seed extract (Nigella sativa) on the growth of Klebsiella pneumoniae and Pseudomonas aeruginosa. The method used was the disc diffusion method. The ethanol extract of black cumin seeds gain by maceration method and was made into various concentration (5, 7.5, 10, 12.5, and 15)%. For positive control, ciprofloxacin disc were used and negative control used distilled water. The research model used was in vitro experimental research. The results showed that ethanol extract of black cumin seeds have antibacterial activity against Klebsiella pneumoniae with inhibition zone diameters (4.5, 6.7, 10.1, 11, 12.8) mm, Pseudomonas aeruginosa with inhibition zone diameters (7.5, 12, 12.6, 13.4, 15.6) mm, positive controls respectively (24.3 and 20.7) mm and negative controls (0). It can be concluded that black cumin seed extract has an inhibiting effect on the growth of Klebsiella pneumoniae and Pseudomonas aeruginosa.

scholarly journals Ethanol Extract of Black Cumin Seed (Nigella Sativa) Reduces Expression Kidney iNOS in Mice Model Preeclampsia

2019 ◽  
Vol 5 (2) ◽  
pp. 88
Author(s):  
Rubiati Hipni
Keyword(s):  
Nigella Sativa ◽  
Ethanol Extract ◽  
Negative Control ◽  
Inos Expression ◽  
Control Group ◽  
Mice Model ◽  
Control Group Design ◽  
Black Cumin ◽  
Black Cumin Seed ◽  
Cumin Seed

This study aims to analyze the effect of ethanol extract of black cumin seeds (Nigella Sativa) on iNOS expression in the kidney of mice model preeclampsia. Design This study uses an experimental design with a posttest only control group design. Using a mice model preeclampsia. This study was divided into 6 groups: negative control, positive control (preeclampsia model), model + black cumin seed extract 500 mg/KgBB/day, 1000 mg/KgBB/day, 1500 mg/KgBB/day, and 2000 mg/KgBB/day days, after surgery on mice then examined iNOS Kidney expression by methods Immunohistochemical. Data from observations were analyzed by one way ANOVA test. The result there is an effect of ethanol extract of black cumin seeds on renal iNOS expression in mice model preeclampsia (p <0.05) at doses of 500mg, 1000mg, 1500mg, and 2000mg on decreasing renal iNOS expression. The conclusion Ethanol extract of black cumin seeds (Nigella sativa) can reduce iNOS Kidney expression in Mice Preeclampsia.

scholarly journals Nigella sativa (Black Cumin) Seed Extract Alleviates Symptoms of Allergic Diarrhea in Mice, Involving Opioid Receptors

PLoS ONE ◽  
2012 ◽  
Vol 7 (6) ◽  
pp. e39841 ◽  
Author(s):  
Swantje C. Duncker ◽  
David Philippe ◽  
Christine Martin-Paschoud ◽  
Mireille Moser ◽  
Annick Mercenier ◽  
...  
Keyword(s):  
Opioid Receptors ◽  
Nigella Sativa ◽  
Seed Extract ◽  
Black Cumin ◽  
Black Cumin Seed ◽  
Cumin Seed

scholarly journals Formulasi dan Uji Aktivitas Antibakteri Sediaan Emulgel Minyak Biji Jintan Hitam (Nigella sativa L.) terhadap Bakteri Staphylococcus epidermidis

2021 ◽  
Vol 11 (2) ◽  
pp. 121-131
Author(s):  
Nesa Agistia ◽  
Melzi Oktaviani ◽  
Wildan Khairi Mukhtadi ◽  
Della Ariska
Keyword(s):  
Antibacterial Activity ◽  
Staphylococcus Epidermidis ◽  
Seed Oil ◽  
Nigella Sativa ◽  
Positive Control ◽  
Diffusion Method ◽  
Black Cumin ◽  
Black Cumin Seed ◽  
Cumin Seed ◽  
Black Cumin Seed Oil

Acne is a skin problem that often occurs, one of the causes is the bacterium Staphylococcus epidermidis. Black cumin seed oil (Nigella sativa L.) can be used as an antibacterial because it contains thymoquinone and α-pinene. This study aims to obtain black cumin seed oil emulgel preparations that has antibacterial activity against Staphylococcus epidermidis. The preparations were formulated with concentrations of 3% (FI), 5% (FII), and 7% (FIII). Evaluation of the preparation was carried out for eight weeks including organoleptic tests, spreadability, emulsion type, pH, viscosity, homogeneity. Antibacterial activity tests was carried out using the well-diffusion method. The results of the evaluation of all three formulas obtained showed a semi-solid consistency, light brown in color, characteristically smelled of black cumin seed oil, stable, M/A emulsion type, homogeneous, non-irritating, pH FI = 4.51-4.95, FII = 4.72-4.99, FIII = 4.57-4.87, dispersion FI = 3.2-3.8 cm, FII = 3.3-3.9 cm, FIII = 3.4-3.9 cm, viscosity FI = 10.7-26.1 Ns/m2, FII = 11.2-32.0 Ns/m2, FIII = 11.5-34.1 Ns/m2. The antibacterial activity test showed the inhibitory power of FI 11,66±0,09 mm, FII 14.48±0.03 mm, FIII 17.35±0,.8 mm, lower than the positive control (clindamycin). All three black cumin seed oil emulgel preparations obtained met the physical requirements and had bacterial inhibition (p

scholarly journals Pengaruh Pemberian Ekstrak Biji Jintan Hitam terhadap Kadar Kolesterol HDL dan LDL Rattus Norvegicus Jantan Galur Wistar yang Diberi Diet Tinggi Lemak

2021 ◽  
Vol 18 (2) ◽  
pp. 204
Author(s):  
RETNO SETYA KEMALA ◽  
CHOESNAN EFFENDI ◽  
ASAMI RIETA KUMALA
Keyword(s):  
Rattus Norvegicus ◽  
Ldl Cholesterol ◽  
Nigella Sativa ◽  
Seed Extract ◽  
Control Group ◽  
Black Cumin ◽  
Cholesterol Levels ◽  
White Rats ◽  
Black Cumin Seed ◽  
Cumin Seed

<p>There are a lot of various kinds of plants such as Black Cumin <em>(Nigella sativa L.)</em> which is believed by the people to increase HDL and decrease LDL levels in the blood.</p><p>The purpose of this experiment<strong> </strong>to know the effect of extract black cumin seeds <em>(Nigella sativa L.)</em>can increase HDL and decrease LDL cholesterol levels of white rats <em>(Rattus norvegicus)</em>.</p><p>This experiment using 24 of rats which divided into 4 groups, namely the negative control group, positive control group, the treatment group one dosage 126 mg/KgBWand the treatment group two dosage 252 mg/KgBW. This research was carried out for 28 days and continued by termination.</p><p>In the One way ANOVA test, HDL levels obtained significant values of 0.134 and LDL levels of 0.070 which means p&gt; 0.05 and showed that black cumin seed extract (Nigella sativa L.) there’s no different.</p><p>The conclusion of the experiment is giving black cumin seed extract <em>(Nigella sativa L.) </em>had no effect for increasing HDL and decreasing LDL cholesterol levels of white rats <em>(Rattus norvegicus)</em>.</p><p><strong>Keywords: </strong> black cumin seed extract, HDL cholesterol levels, LDL cholesterol level, <em>(Nigella sativa L.)</em>.</p><p><strong> </strong></p>

scholarly journals BLACK CUMIN (NIGELLA SATIVA) EFFECT ON EXPRESSION OF TNF-α, IL-2, DANsFlt-1IN PREECLAMPTIC MODEL RATS

2020 ◽  
pp. 78-86
Author(s):  
JOHNY MARPAUNG ◽  
M. F. G. SIREGAR ◽  
MAKMUR SITEPU ◽  
ADANG BACHTIAR
Keyword(s):  
Analytical Study ◽  
Nigella Sativa ◽  
Seed Extract ◽  
Control Group ◽  
Control Group Design ◽  
Black Cumin ◽  
Tnf Α ◽  
Black Cumin Seed ◽  
Cumin Seed ◽  
Normally Distributed

Objective: To prove the effect of giving black cumin seed extract (Nigella sativa) to decrease levels of TNF-α, IL-2, sFlt-1, and in PE model mice. Methods: This study is an analytical study with true experimental research design in laboratory mice (Rattus norvegicus) pregnant female has given black cumin seed extract (Nigella sativa) at a dose of 500 mg/kg/day and 2000 mg/kg/day. The treatment of all samples was carried out simultaneously and during the treatment was observed using the type of Postest Only Control Group Design. To assess whether the sample is normally distributed or not the Shapiro-Wilk test was carried out because the sample was ≤50. To assess the comparison of parameters (differences in expression of IL-2, sFlt-1, and TNF-α) between groups, the ANOVA test was used if the data were normally distributed and the Kruskal Wallis test if the data were not normally distributed. Results: From these results, it can be seen that there are significant differences in the administration of black cumin extract on TNF-α, IL-2, andsFlt-1 expression (p<0.001); the administration of black cumin extract can reduce TNF-α, IL-2, and sFlt-1 expression in preeclampsia mice. The administration of 2000 mg black cumin extract can reduce TNF-α and IL-2 expression more significantly. Conclusion: Black cumin can reduce levels of TNF-α, IL-2, sFlt-1 in models mice with preeclampsia (p<0.001).

scholarly journals PENGARUH KRIM EKSTRAK JINTAN HITAM (Nigella sativa) TERHADAP KADAR KOLAGEN DAN HIDRASI KULIT PADA TIKUS (Rattus norvegicus) GALUR WISTAR JANTAN YANG DIPAPAR SINAR ULTRAVIOLET-B

2020 ◽  
Vol 19 (3A) ◽  
Author(s):  
Winda Sari ◽  
Linda Chiuman ◽  
Sahna Ferdinand Ginting ◽  
Chrismis Novalinda Ginting
Keyword(s):  
Rattus Norvegicus ◽  
Nigella Sativa ◽  
Seed Extract ◽  
Ultraviolet B ◽  
Oxygen Species ◽  
Black Cumin ◽  
Wistar Strain ◽  
Before And After ◽  
Black Cumin Seed ◽  
Cumin Seed

Photoaging is caused by exposure to sunlight and ultraviolet radiation (UV-B) through the reactive oxygen species (ROS) molecules formation, resulting in decreased levels of collagen. It is characterized by wrinkles, sagging and rough surfaces. Black cumin has been used in medicine due to its high antioxidant potential. The aim of this study is to identify the effect of black cumin cream on collagen levels and hydration of the skin of Rattus norvegicus exposed to ultraviolet-B rays. This is experimental study in which 30 male Wistar strain rats were classified into 6 sub-groups, namely the KN group (without treatment), KP (getting Nivea cream moisturizer), P1 (basic cream ), P2 (black cumin seed extract cream 0.25%), P3 (cream 0.5%) and P4 (cream 0.75%). P4 rat collagen levels increased significantly before and after the intervention (24 ± 2.23 vs 42.6 ± 14.6, p <0.05).Cream with lower concentrations did not provide a significant increase (p> 0.05). P4 rats also showed increased skin hydration (38.6 ± 19.3 to 63.0 ± 4.47 (p <0.05)), and this was not found in other groups. The administration of topical black cumin seed extract 0.75% can inhibit decreased levels of collagen and increase skin tissue hydration.   

scholarly journals UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL RIMPANG KUNYIT (Curcuma longa L.) TERHADAP Staphylococcus aureus DAN Pseudomonas aeruginosa

2019 ◽  
Vol 4 (1) ◽  
pp. 278-287
Author(s):  
Lia Fikayuniar ◽  
Neni Sri Gunarti ◽  
Mellya Apriliani
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Curcuma Longa ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Negative Control ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Paper Disk

ABSTRAK Kunyit (Curcuma longa L.) merupakan salah satu jenis tanaman obat yang termasuk dalam keluarga Zingiberaceae. Senyawa aktif yang terkandung dalam rimpang kunyit (Curcuma longa L.) mampu bekerja sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri dari ekstrak etanol rimpang kunyit (Curcuma longa L.) terhadap Staphylococcus aureus dan Pseudomonas aeruginosa. Ekstraksi dilakukan dengan cara refluks menggunakan pelarut etanol 96%. Pengujian aktivitas antibakteri dilakukan menggunakan metode difusi paper disk dengan masing-masing konsentrasi ekstrak 10%, 20%, 30%, 40% b/v. Kontrol positif yang digunakan adalah Ciprofloxacin sedangkan kontrol negatif yang digunakan adalah DMSO. Hasil skrining fitokimia ekstrak etanol rimpang kunyit mengandung alkaloid, flavonoid, fenol, tanin dan terpenoid. Berdasarkan hasil penelitian, ekstrak etanol rimpang kunyit dapat menghambat bakteri Staphylococcus aureus dan Pseudomonas aeruginosa pada konsentrasi 10%, 20%, 30%, 40% dan konsentrasi 40% merupakan konsentrasi yang memberikan diameter zona hambat terbesar terhadap kedua bakteri uji yaitu 8,63 mm dan 7,8 mm. Kata Kunci : aktivitas antibakteri, Curcuma longa L., Staphylococcus aureus,    ABSTRACT Turmeric (Curcuma longa L.) is one type of medicinal plant that belongs to the Zingiberaceae family. The active compounds contained in the Curcuma longa L. rhizome can work as antibacterial. This study aims to determine the antibacterial activity of the ethanol extract of Curcuma longa L. Antibacterial activity testing was carried out using the paper disk diffusion method with each extract concentration of 10%, 20%, 30%, 40%. The positive control used was Ciprofloxacin while the negative control used was DMSO. The results of phytochemical screening of the ethanol extract of Curcuma longa L. rhizome contain alkaloids, flavonoids, phenols, tannins and terpenoids. Based on the results of the study, the ethanol extract of turmeric rhizome can inhibit Staphylococcus aureus and Pseudomonas aeruginosa bacteria at concentrations of 10%, 20%, 30%, 40% and 40% concentrations which give the largest inhibition zone diameter of the two test bacteria which is 8.63 mm and 7.8 mm. Keywords: antibacterial activity, Curcuma longa L., Staphylococcus aureus,  

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