scholarly journals Synthesis of Novel Glycerol-Derived 1,2,3-Triazoles and Evaluation of Their Fungicide, Phytotoxic and Cytotoxic Activities

2017 ◽  
Author(s):  
Adilson Costa ◽  
Marcos Vinicius Lacerda Oliveira ◽  
Roberta Tristão Pinto ◽  
Luiza Carvalheira Moreira ◽  
Ediellen Mayara Corrêa Gomes ◽  
...  
Keyword(s):  
Large Scale ◽  
High Efficiency ◽  
Click Reaction ◽  
Biodiesel Production ◽  
Cytotoxic Activities ◽  
New Agents ◽  
Phytotoxic Effect ◽  
Fungicide Activity ◽  
Commercial Fungicide

Glycerol is a subproduct of biodiesel production and represents an important problem when generated in large scale. Alternatives that can utilize this unrefined byproduct is of potential interest. It is herein described the synthesis of a series of 1,2,3-triazoles using glycerol as starting material. The key step involved in the preparation of triazolic derivatives corresponded to the Copper(I)-catalyzed Azide-Alkyne Cycloaddition (CuAAC), also known as click reaction, between 4-(azidomethyl)-2,2-dimethyl-1,3-dioxolane (3) and different terminal alkynes. The eight prepared derivatives were evaluated with regard to their fungicide, phytotoxic and cytotoxic activities. The fungicide activity was assessed in vitro against Colletotrichum gloeosporioides, the causing agent of papaya anthracnose. It was found that the compounds 1-(1-((2,2-dimethyl-1,3-dioxolan-4-yl)methyl)-1H-1,2,3-triazol-4-yl)cyclohexanol (4g) and 2-(1-((2,2-dimethyl-1,3-dioxolan-4-yl)methyl)-1H-1,2,3-triazol-4-yl)propan-2-ol (4h) demonstrated high efficiency on controlling C. gloeosporioides when compared to the commercial fungicide tebuconazole. The triazoles did not present any phytotoxic effect when evaluated against Lactuca sativa. However, five derivatives were mitodepressive, inducing cell death detected by the presence of condensed nuclei and acted as aneugenic agents in the cell cycle of L. sativa. It is believed that glycerol derivatives bearing 1,2,3-triazole functionalities may represent a scaffold to be explored toward the development of new agents to control C. gloeosporioides.

Novel Materials for Myco-Decontamination of Cyanide-Containing Wastewaters through Microbial Biotechnology

2021 ◽  
Vol 1037 ◽  
pp. 751-758
Author(s):  
Igor N. Pavlov ◽  
Yulia A. Litovka
Keyword(s):  
Large Scale ◽  
High Efficiency ◽  
Pine Sawdust ◽  
Microbial Biotechnology ◽  
Tailing Dam ◽  
Large Scale Cultivation ◽  
Siberian Pine ◽  
Uniform Ratio

This study examined the effectiveness of decontamination of industrial cyanide-containing water using mycelium-based lignocellulosic materials. These results suggest that fungi biomass and plant substrates can be used successfully in the treatment of wastewater contaminated by cyanide. Fungi were isolated from old wood samples taken from a tailing dam with high cyanide content (more than 20 years in semi-submerged condition). All isolated fungi belonged to the genus Fusarium. Fusarium oxysporum Schltdl. is most effective for biodegradation of cyanide-containing wastewaters (even at low temperatures). The most optimal lignocellulosic composition for production of mycelium-based biomaterial for biodegradation of cyanide wastewater consists of a uniform ratio of Siberian pine sawdust and wheat straw. The high efficiency of mycelium-based materials has been experimentally proven in vitro at 15-25 ° C. New fungal biomaterials are provide decrease in the concentration of cyanide ions to 79% (P <0.001). Large-scale cultivation of fungi biomass was carried out by the periodic liquid-phase cultivation. The submerged biomass from bioreactor was used as an inoculum for the production of mycelium-based materials for bioremediation of cyanide wastewater in situ (gold mine tailing).

scholarly journals High Efficiency Secondary Somatic Embryogenesis inHovenia dulcisThunb. through Solid and Liquid Cultures

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Jingli Yang ◽  
Songquan Wu ◽  
Chenghao Li
Keyword(s):  
Somatic Embryogenesis ◽  
Somatic Embryos ◽  
Large Scale ◽  
High Efficiency ◽  
Secondary Embryogenesis ◽  
Secondary Somatic Embryogenesis ◽  
Liquid Cultures ◽  
Medicinal Tree ◽  
Plant Conversion

Embryogenic callus was obtained from mature seed explants on medium supplemented with 2,4-dichlorophenoxyacetic acid. Primary somatic embryos (SEs) can only develop into abnormal plants. Well-developed SEs could be obtained through secondary somatic embryogenesis both in solid and liquid cultures. Temperature strongly affected induction frequency of secondary embryogenesis. Relatively high temperature (30∘C) and germinated SEs explants were effective for induction of secondary somatic embryos, and low temperature (20∘C) was more suitable for further embryo development, plantlet conversion, and transplant survival. Somatic embryos formed on agar medium had larger cotyledons than those of embryos formed in liquid medium. Supplementing 0.1 mg L−16-benzyladenine (BA) was effective for plant conversion; the rate of plant conversion was 43.3% in somatic embryos from solid culture and 36.5% in embryos from liquid culture.In vitroplants were successfully acclimatized in the greenhouse. The protocol established in this study will be helpful for large-scale vegetative propagation of this medicinal tree.

Green Synthesized Silver Nanoparticles: A Promising Anticancer Agent

2020 ◽  
Vol 19 (04) ◽  
pp. 1950027 ◽  
Author(s):  
Samuel Shiferaw Biresaw ◽  
Samrawit Mekonnen Damte ◽  
Pankaj Taneja
Keyword(s):  
Silver Nanoparticles ◽  
High Efficiency ◽  
Synergistic Effects ◽  
In Vivo Model ◽  
Cytotoxic Activities ◽  
Coating Agent ◽  
Synthesis Strategy ◽  
Physical And Chemical

Silver nanoparticles (AgNPs) have attracted a great deal of attention in the recent years. It is mostly due to their availability, chemical stability, catalytic activity, conductivity, biocompatibility and anticancer activity. There are three major approaches for AgNPs synthesis; i.e., chemical, physical, and biological methods. Today, many chemical and physical methods have become less popular due to usage of hazardous chemicals or their high costs, respectively. The green method has introduced an appropriate substitute synthesis strategy for the conventional physical and chemical approaches. The utilization of the plant extracts as reducing, stabilizing and coating agent of AgNPs is an interesting eco-friendly approach leading to high efficiency. The anticancer synergistic effects among the AgNPs and phytochemicals will enhance their therapeutic potentials. Surprisingly, although many studies have demonstrated the significant enhancement in cytotoxic activities of plant-mediated AgNPs toward cancerous cells, these nanoparticles (NPs) have been found nontoxic to normal human cells in their therapeutic concentrations. This paper provides a specific insight into the mechanism of plant-mediated AgNPs synthesis, their anticancer and cytotoxic activities in vitro cancer cells, in vivo model animals and clinical trials.

scholarly journals Bioactivity Screening and Identification of Secondary Metabolites from Fungal Endophytes of Carica papaya L. Leaves

2021 ◽  
Vol 20 (4) ◽  
pp. 217
Author(s):  
Israt Farha Lini ◽  
Rabita Zinnurine ◽  
Md. Habibur Rahman ◽  
Mst. Nadira Begum ◽  
Farhana Afroz ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Endophytic Fungi ◽  
Large Scale ◽  
Carica Papaya ◽  
Fungal Endophytes ◽  
Diffusion Method ◽  
Cytotoxic Activities ◽  
Alternariol Monomethyl Ether ◽  
Chemical Screening

<strong>Objectives:</strong> This work describes the chemical and <em>in-vitro</em> bioactivity studies on endophytic fungi derived from the leaves of <em>Carica papaya</em> L. <strong>Methods:</strong> Column Chromatography was employed for isolation of fungal metabolites. Nuclear Magnetic Resonance (NMR) spectral data was utilized to elucidate the structure of the isolated compounds and physicochemical properties of them were also examined. As a part of<em> in-vitro</em> bioactivity screening disc diffusion method, 1, 1-diphenyl-2- picryl-hydrazyl (DPPH) scavenging assay and brine shrimp lethality bioassay were conducted to evaluate antimicrobial, antioxidant and cytotoxic activities, respectively.<strong> Results:</strong> A total of four fungal isolates were identified as endophytic fungi and purified from the young leaves of <em>Carica papaya</em>. These fungi, encoded as CPLE-1, CPLE-2, CPLE-3 and CPLE-4, were identified up to the genus level on the basis of their macroscopic and microscopic characteristics as <em>Colletotrichum</em> sp., <em>Carvularia</em> sp., and next two as <em>Alternaria</em> sp., respectively. Chemical profiling and preliminary bioactivity screening of the fungal endophytes signified strain CPLE-3 with highest potentiality to produce bioactive compounds which directs its large scale cultivation. Ergosterol and Alternariol monomethyl ether were isolated from the ethyl acetate extract of the fungus, CPLE-3. <strong>Conclusion:</strong> Bioactivity and chemical screening of the endophytic fungal extracts of <em>Carica papaya</em> leaf along with the isolation of fungal secondary metabolites suggested these endophytic fungi to be possible source of bioactive leads for developing new and improved medicines.

scholarly journals Tumor-microenvironment Responsive Targeted Exosome-like Nanovesicles From Dunaliella Salina for Enhancing Gene/immune Combination Therapy

2021 ◽  
Author(s):  
Mengxi Zhu ◽  
Shan Li ◽  
Shuying Feng ◽  
Haojie Wang ◽  
Lina Hu ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Delivery System ◽  
Targeted Delivery ◽  
Large Scale ◽  
Dunaliella Salina ◽  
High Efficiency ◽  
Particle Diameter ◽  
Matrix Metalloproteinase 2 ◽  
Scale Production

Abstract BackgroundAs an endogenous extracellular vesicle, exosome is increasingly presenting its great potential in the field of drug delivery. However, it is the bottleneck to obtain a large number of uniform, stable and multi-component controllable exosomes with low cost and time. ResultsIn this study, we develop a novel targeted drug delivery system based on exosome-like nanovesicle by use of natural marine single-celled Dunaliella salina (DENV), the c(RGDyK) peptide has been conjugated to DENV surface to achieve the targeted delivery to esophageal cancer cells. Furthermore, miR-375 has been loaded into the cRGD-DENV through electroporation, and aPD-L1 has been conjugated onto its surface via Gly-PLGLAG-Cys peptide, a matrix metalloproteinase-2 (MMP-2)-cleavable peptide, which facilitates the release of aPD-L1 in tumor environment to achieve the high-efficiency combination of gene therapy and immunotherapy. Firstly, the engineered DENV delivery system was prepared and characterized. It exhibited a proper particle diameter (approximately 150 nm) with in vitro sustained release features in the presence of MMP-2/9. More importantly, the cRGD-DENV was effective, promoted selective delivery of cargo to the target site, and reduced nonspecific uptake, consequently, significantly inhibit tumor growth in vitro and in vivo. ConclusionThe specific nanocarrier delivery system provide a promising strategy for the rapid and large-scale production of functionalized exosome-like nanovesicle by adapting multifunctional peptides specifically targeted tumor.

scholarly journals Fast Gelation of Poly(ionic liquid)-Based Injectable Antibacterial Hydrogels

Gels ◽  
2022 ◽  
Vol 8 (1) ◽  
pp. 52
Author(s):  
Che Zhao ◽  
Chengju Sheng ◽  
Chao Zhou
Keyword(s):  
Antibacterial Agents ◽  
High Efficiency ◽  
Click Reaction ◽  
Umbilical Vein ◽  
Antibacterial Properties ◽  
Bactericidal Effect ◽  
Wound Dressings ◽  
Vitro Assay ◽  
Propiolic Acid

Traditional antibacterial hydrogels have a broad-spectrum bactericidal effect and are widely used as wound dressings. However, the biological toxicity and drug resistance of these antibacterial hydrogels cannot meet the requirements of long-term clinical application. Imidazolium poly(ionic liquids) (PILs) are polymeric antibacterial agents exhibiting strong antibacterial properties, as they contain a strong positive charge. In this study, two imidazolium PILs, namely poly(N-butylimidazolium propiolic acid sodium) (PBP) and poly(N-(3,6-dioxaoctane) imidazolium propiolic acid sodium) (PDP), as high efficiency antibacterial agents, were synthesized by polycondensation reaction. Then, the PILs were compounded with polyethylene glycol (PEG) by a thiol-yne click reaction to prepare injectable antibacterial hydrogels. An in vitro assay showed that the injectable antibacterial hydrogels could not only quickly kill Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), but also had low toxicity for human skin fibroblasts cells (HSFs) and human umbilical vein endothelial cells (HUVECs), respectively. Additionally, the lipopolysaccharide (LPS) inflammation model revealed that the injectable antibacterial hydrogels also had anti-inflammatory effects, which would be advantageous to accelerate wound healing.

Purification of the Antihemophilic Factor by Gel Filtration on Agarose

1969 ◽  
Vol 22 (03) ◽  
pp. 577-583 ◽  
Author(s):  
M.M.P Paulssen ◽  
A.C.M.G.B Wouterlood ◽  
H.L.M.A Scheffers
Keyword(s):  
Factor Viii ◽  
Plasma Proteins ◽  
Large Scale ◽  
Gel Filtration ◽  
Large Scale Preparation ◽  
Scale Preparation ◽  
Antihemophilic Factor

SummaryFactor VIII can be isolated from plasma proteins, including fibrinogen by chromatography on agarose. The best results were obtained with Sepharose 6B. Large scale preparation is also possible when cryoprecipitate is separated by chromatography. In most fractions containing factor VIII a turbidity is observed which may be due to the presence of chylomicrons.The purified factor VIII was active in vivo as well as in vitro.

The Potential of Photoelectrochemical Carbon Sinks

2018 ◽  
Author(s):  
Matthias May ◽  
Kira Rehfeld
Keyword(s):  
Global Warming ◽  
High Temperature ◽  
Greenhouse Gas ◽  
Large Scale ◽  
High Efficiency ◽  
Carbon Sink ◽  
Solar Fuels ◽  
Negative Emissions ◽  
Viable Approach ◽  
Low Sensitivity

Greenhouse gas emissions must be cut to limit global warming to 1.5-2C above preindustrial levels. Yet the rate of decarbonisation is currently too low to achieve this. Policy-relevant scenarios therefore rely on the permanent removal of CO<sub>2</sub> from the atmosphere. However, none of the envisaged technologies has demonstrated scalability to the decarbonization targets for the year 2050. In this analysis, we show that artificial photosynthesis for CO<sub>2</sub> reduction may deliver an efficient large-scale carbon sink. This technology is mainly developed towards solar fuels and its potential for negative emissions has been largely overlooked. With high efficiency and low sensitivity to high temperature and illumination conditions, it could, if developed towards a mature technology, present a viable approach to fill the gap in the negative emissions budget.<br>

The Potential of Photoelectrochemical Carbon Sinks

2018 ◽  
Author(s):  
Matthias May ◽  
Kira Rehfeld
Keyword(s):  
Global Warming ◽  
High Temperature ◽  
Greenhouse Gas ◽  
Large Scale ◽  
High Efficiency ◽  
Carbon Sink ◽  
Solar Fuels ◽  
Negative Emissions ◽  
Viable Approach ◽  
Low Sensitivity

Greenhouse gas emissions must be cut to limit global warming to 1.5-2C above preindustrial levels. Yet the rate of decarbonisation is currently too low to achieve this. Policy-relevant scenarios therefore rely on the permanent removal of CO<sub>2</sub> from the atmosphere. However, none of the envisaged technologies has demonstrated scalability to the decarbonization targets for the year 2050. In this analysis, we show that artificial photosynthesis for CO<sub>2</sub> reduction may deliver an efficient large-scale carbon sink. This technology is mainly developed towards solar fuels and its potential for negative emissions has been largely overlooked. With high efficiency and low sensitivity to high temperature and illumination conditions, it could, if developed towards a mature technology, present a viable approach to fill the gap in the negative emissions budget.<br>

Peculiarities of the phytosanitary state of pome fruits in industrial horticulture

2020 ◽  
Vol 60 (1) ◽  
pp. 159-168
Author(s):  
V. V. Antonenko ◽  
A. V. Zubkov ◽  
S. N. Kruchina
Keyword(s):  
Mechanism Of Action ◽  
Large Scale ◽  
High Efficiency ◽  
Fruit Trees ◽  
Fruit Crops ◽  
Planting Material ◽  
Relative Safety ◽  
Pome Fruits ◽  
Weed Plants ◽  
Spread Of Infection

Data were obtained on the basis of the results of research carried out on the territory of the educational and experimental farm of the Timiryazev State Agrarian University, in Moscow during 2018-2019. As a result of the surveys, the most dangerous diseases and pests of pome crops on the territory of this farm were established. The most resistant apple and pear varieties to major diseases have been identified. Peculiarities of development of alternariosis on pear are described, the harmfulness of the disease on pear and apple seedlings is noted. A possible role in the transfer of alternariosis infection from garden-protective plantations and weed vegetation to fruit trees was noted. A possible role has been established in the transport of septoriosis, powdery dew infection from dicotyledonous weeds plants. The peculiarities of the spread of infection under the influence of wind direction are noted. The results and peculiarities of the application of various methods of scaring birds in the orchard are presented. As a result of route surveys the most harmful weed plants have been identified. The possibility of using herbicides of different mechanism of action in fruit gardens for weed control has been studied. High efficiency and relative safety of application of herbicides of contact action in nursery fields, operational orchards and for control of piglets on fruit trees are shown. Recommendations are given for the use of soil and systemic herbicides of soil in seedlings beds, the first and second fields of the nursery, as well as in the process of production of large-scale planting material and operational orchards of fruit crops. The safety of the herbicides in question is established when used in accordance with the recommended methods of use.

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