Optimization of Extraction of D-pinitol and Phenolics from Cultivated and Wild Types of Carob Pods Using Response Surface Methodology

2011 ◽  
Vol 7 (6) ◽  
Author(s):  
Irfan Turhan
Keyword(s):  
Response Surface Methodology ◽  
Phenolic Compounds ◽  
Dilution Rate ◽  
Response Surface ◽  
Extraction Time ◽  
Total Phenolic ◽  
Extraction Temperature ◽  
Total Phenolic Compounds ◽  
Beneficial Effects ◽  
Carob Pod

The carob pod, an important fruit of the food industry in the production of concentrated syrup, is rich in potentially health-promoting phenolic compounds and sugars. Moreover, one of the important bioactive components of carob is D-pinitol, and it has some beneficial effects on human metabolism. The optimum conditions for extraction of D-pinitol and total phenolic compounds (TPC) from cultivated and wild types of carob pods were determined using response surface methodology (RSM). The Box-Behnken Design (BBD) was used to investigate the effects of three independent variables; extraction temperature (ºC), dilution rate (w/v), and extraction time (h) on the response, D-pinitol and the total phenolic compounds. The optimum extraction conditions obtained using the response optimizer were an extraction temperature of 80ºC, a dilution rate of 1:4, and an extraction time of 2h. Under the mentioned above conditions, the maximum D-pinitol and TPC concentrations were 9.67 g/L and 5916.55 mg/L in wild carob pod extract, respectively.

scholarly journals Development of Phaleria macrocarpa (Scheff.) Boerl Fruits Using Response Surface Methodology On Phenolic, Flavonoid and Antioxidants Properties

2018 ◽  
Author(s):  
Khurul Ain Mohamed Mahzir ◽  
Siti Salwa Abdul Gani ◽  
Nor Fadzillah Mokhtar
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Extraction Time ◽  
Feed Ratio ◽  
Total Phenolic ◽  
Extraction Temperature ◽  
Phaleria Macrocarpa

In this study, the optimal condition for the extraction of antioxidants from the fruit Buah Mahkota Dewa (Phaleria macrocarpa) was determined by using Response Surface Methodology (RSM). The optimization was applied using central composite design (CCD) to investigate the three independent variables, namely extraction temperature (oC), extraction time (minutes) and extraction solvent to-feed ratio (%v/v) on the responses of free radical scavenging activity (DPPH), ferric ion reducing power assay (FRAP), total phenolic content (TPC) and total flavonoid content (TFC).The optimal conditions for the antioxidants extraction were found to be extraction temperature (64oC), extraction time (66 minutes) and solvent to-feed ratio (75 %v/v) with the highest percentage yield of DPPH, FRAP, TPC and TFC were 86.85%, 7.47%, 292.86 mg/g and 3.22 mg/g respectively. Moreover, the data were subjected to response surface methodology (RSM) and the results showed that the polynomial equations for all models were significant, did not show lack of fit, and presented adjusted determination coefficients (R2) above 99%, proving the yield of phenolic, flavonoid and antioxidants activities obtained experimentally were close to the predicted values and the suitability of the model employed in RSM to optimize the extraction conditions. Hence, in this study, the fruit from P.macrocarpa could be considered to have the strong antioxidant ability and can be used in various cosmeceutical or medicinal applications.

Development of a Sterilized Mixed Herbal Drink Product

2009 ◽  
Vol 5 (3) ◽  
Author(s):  
Bodin Techaratanakrai ◽  
Kalaya Laohasongkram ◽  
Saiwarun Chaiwanichsiri
Keyword(s):  
Antioxidant Activity ◽  
Response Surface Methodology ◽  
Phenolic Compounds ◽  
Weight Ratio ◽  
Total Phenolic ◽  
Extraction Temperature ◽  
Herbal Extracts ◽  
Total Phenolic Compounds ◽  
Extraction Condition ◽  
Total Potassium

A sterilized mixed herbal drink formula and process was developed for the mixed herbal extracts from Imperata cylindrical (L.) P. Beauv [IC], Murdannia loriformia (Hassk.) Rolla Rao et Kammathy [ML], Hedyotis corymbosa Lamk. [HC] and Orthosiphon aristatus Miq [OA]. Increasing extraction temperature and time decreased the antioxidant activity and increased the total potassium and phenolic compound contents of the herb infusions. Using response surface methodology (RSM), the appropriate extraction condition was 74.6°C for 41 minutes. The most acceptable formula contains a % weight ratio (w/w) of 14:35:27:17of IC: ML: HC: OA infusions to 7% (w/w) sucrose. Decreasing the sterilization temperature and increasing the F0-value reduced the antioxidant activity and total phenolic compounds. The appropriate sterilization temperature and F0 were 135°C and 3 minutes.

scholarly journals Optimization of Extraction of Total Phenolic and Total Flavonoid from Seeds of Alpinia blepharocalyx K. Schum. Using The Response Surface Methodology

2019 ◽  
Vol 57 (3B) ◽  
pp. 105
Author(s):  
Thanh Tan Nguyen ◽  
Huyen Thi Thanh Mai ◽  
Tuan Ngoc Nguyen ◽  
Quang Dinh Tran ◽  
Thang Dinh Tran
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Antioxidant Properties ◽  
Total Flavonoid ◽  
Extraction Time ◽  
Total Phenolic ◽  
Extraction Temperature ◽  
Flavonoid Compounds ◽  
Phenolic And Flavonoid ◽  
Phenolic And Flavonoid Compounds

Seeds of Alpinia blepharocalyx contain high amounts of phenolic and flavonoid compounds with potential antioxidant properties. In this study, the effects of the extraction method on the recovery of phenolic and flavonoid compounds from Alpinia blepharocalyx seeds were investigated. Response surface methodology (RSM) has been used to optimize the extraction conditions of total phenolic and total flavonoid from seeds of Alpinia blepharocalyx. A Box-Behnken design was used to investigate the effects of four independent variables, namely extraction temperature (°C), extraction time (min), (v/m) and ethanol concentration (%) on the responses: total phenolic content (TPC) and yield. The optimal conditions obtained from response RSM were 52.66 % v/v for the solvent composition, 62.34 (°C) for extraction temperature, 34.48/1 (ml/g) for solvent/material ratio and 125.42 (min) for extraction time. The experimental values of TPC, TFC and yield were 39.31±0.05 mgGAE/g, 12.75±0.07 mgCE/g and 6.97±0.05%, respectively.   

scholarly journals Optimization of Ultrasound-Assisted Extraction of Phenolics from Sideritis raeseri Using Response Surface Methodology

Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 3949
Author(s):  
Katarina Šavikin ◽  
Jelena Živković ◽  
Teodora Janković ◽  
Nada Ćujić-Nikolić ◽  
Gordana Zdunić ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Ethanol Concentration ◽  
Extraction Time ◽  
Total Phenolic ◽  
Solvent Ratio ◽  
Extraction Temperature ◽  
Ultrasound Assisted Extraction ◽  
Assisted Extraction ◽  
Ultrasound Assisted

In this study we define the optimal conditions for ultrasound-assisted extraction of bioactive polyphenols from S. raeseri aerial parts using response surface methodology. The influence of ethanol concentration (10–90%), extraction temperature (20–80 °C), extraction time (10–60 min), and solid-to-solvent ratio (1:10–1:50) on total phenolic content as well as on content of individual flavonoids, and hypolaetin and isoscutellarein derivatives was studied. For the experimental design, a central composite design was chosen. In the obtained extracts, the following ranges of targeted compounds were detected: total phenol from 19.32 to 47.23 mg GAE/g dw, HYP from 1.05 to 11.46 mg/g dw, ISC 1 from 0.68 to 10.68 mg/g dw, and ISC 2 from 0.74 to 15.56 mg/g dw. The optimal extraction conditions were set as: ethanol concentration of 65%, extraction time of 50 min, extraction temperature of 63 °C, and solid-to-solvent ratio of 1:40. Contents of TP, HYP, ISC 1, and ISC 2 in optimal extracts were 47.11 mg GAE/g dw, 11.73 mg/g dw, 9.54 mg/g dw, and 15.40 mg/g dw, respectively. Experimentally set values were in good agreement with those predicted by the response surface methodology model, indicating suitability of the used model, as well as the success of response surface methodology in optimizing the conditions of the extraction.

EXTRACTION OPTIMIZATION OF THREE INDEPENDENT VARIABLES OF ANTIOXIDANTS FROM BASIL LEAVES USING RESPONSE SURFACE METHODOLOGY

INDIAN DRUGS ◽  
2020 ◽  
Vol 57 (04) ◽  
pp. 20-28 ◽  
Author(s):  
Sulaiman M. Alnasser ◽  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Radical Scavenging ◽  
Extraction Yield ◽  
Extraction Time ◽  
Total Phenolic ◽  
Extraction Temperature ◽  
Optimum Conditions ◽  
Antioxidant Power ◽  
Independent Variables

The current study reports the antioxidant activity of Ocimum basilicum. Central composite design (CCD) was used to study the effect of variables on the extraction using magnetic stirrer (MST). Three independent variables including temperature, extraction speed, and extraction time were studied that optimize particular responses of total phenolic content, ferric reducing antioxidant power (FRAP), and DPPH free radical scavenging activity. The effect of the extraction temperature on extraction process was investigated in the range from 26.3oC to 93.6oC, extraction time, from 1 to 30 h, and extraction speed, in the range from 263 to 936 rpm. The extraction yield was significantly (P < 0.05) affected by all the variables. Temperature and extraction speed were found to have a marked effect while the extraction time was found to have possible minor effects. Graphical optimization determined the optimum conditions for the extraction. The optimum condition predicted an extraction yield of 20.49g/100g at 75.33oC for five hours at 73 rpm. Optimum conditions were determined to obtain highest extraction yield. Results showed that water/seed ratio was the most significant parameter, followed by temperature and time. All quantitative modeling and response surface methodology recommended that extraction temperature and time were the most effective parameters of MST process. However, extraction time was found out to be an insignificant factor in MST extraction of antioxidant and total phenolic compounds of O. basilicum.

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