Tracking the Healing Process of Experimental Liver Injuries Treated with Different Sealants and Adhesive Biomaterials: An Evaluation of Matrix Metalloproteinase

This study examines the matrix metalloproteinase (MMP) activity in the healing of liver injuries treated with biological adhesives Tachosil® and GelitaSpon® and the elastic cyanoacrylate Adhflex®. Hepatic lesions were induced in male rats using a Stiefel biopsy punch. Healing was assessed 2, 6, and 18 days after injury by quantifying tissue levels of MMP1, 2, 8, 9, and 13. Histopathological repair was evaluated using hematoxylin-eosin, Masson’s trichrome, and Periodic Acid Schiff (PAS) staining and immunohistochemical markers CD31 and CD68. The sealants contributed to complete healing. Histopathology and MMP findings indicate that Adhflex® has slower degradation and a strong inflammatory reaction at the onset of healing. Early on, all MMPs showed higher levels in Adhflex® and Tachosil®-treated animals, and MMP2 and MMP9 expressions were significantly higher in the Adhflex-treated group at 18 days post-injury (T3). The Adhflex® group had significantly higher MMP8 and MPP13 levels than other treated groups and showed a sustained overexpression of all MMPs, even in the latest healing stages. Notably, the overexpression did not negatively influence the histological healing process. All hepatic trauma injuries should be treated as emergencies, and any easy-to-use and rapid sealant like Adhflex® could be considered as an option for treating liver trauma.

Download Full-text

Tracking Healing Process of Experimental Liver Injuries Treated with Different Sealants and Adhesives Biomaterials. Matrix Metalloproteinase Evaluation

10.20944/preprints201806.0334.v2 ◽

2018 ◽

Author(s):

José Miguel Lloris-Carsí ◽

Carlos Barrios-Pitarque ◽

José Miguel Lloris-Cejalvo ◽

Luis Gil-Santos ◽

Dolores Cejalvo-Lapeña

Keyword(s):

Wound Healing ◽

Periodic Acid ◽

Healing Process ◽

Treated Group ◽

Complete Healing ◽

Male Rats ◽

Periodic Acid Schiff ◽

Adequate Treatment ◽

Liver Injuries ◽

Hepatic Lesions

. Sealants and adhesives are used in the repair and preservation of damaged solid organs. This study examines the matrix metalloproteinases (MMP) activity in the healing of liver injuries treated with two biological adhesives (Tachosil® and GelitaSpon®) as well as that of a new elastic cyanoacrylate (Adhflex®). Methods. We induced in 90 male rats hepatic lesions using a Stiefel biopsy punch in the liver. Wound healing was assessed 2, 6, and 18 days after injury by quantifying MMP1, 2, 8, 9, and 13 tissue levels. The histopathological repair was evaluated by hematoxylin-eosin, Masson’s trichrome, and Periodic Acid Schiff (PAS) staining and CD31, CD68 immunohistochemical marker. The three sealants used contributed to the complete healing of hepatic lesions. Both histopathology and MMP findings point to the fact that degradation with Adhflex® is slower and causes a strong inflammatory reaction at the onset of healing. Results. All the MMPs measured showed higher values early in the healing process in animals treated with Adhflex® and Tachosil, expression for MMP2 and MMP9 being significantly higher in the Adhflex-treated group. Animals treated with Tachosil had significant greater values of MMP8 and MPP13 than the Adhflex group. Animals treated with Adhflex® showed a sustained overexpression in all MMPs even at the latest wound healing stages. Conclusion. Notably, the overexpression of the MMPs did not negatively influence the histological healing process of liver injuries. Since all hepatic trauma injuries should be treated as emergencies, any easy-to-use and rapid sealant, like Adhflex®, could be considered an adequate treatment option.

Download Full-text

Tracking Healing Process of Experimental Liver Injuries Treated with Different Sealants and Adhesives Biomaterials. Matrix Metalloproteinase Evaluation

10.20944/preprints201806.0334.v1 ◽

2018 ◽

Author(s):

José Miguel Lloris Carsí ◽

Carlos Barrios-Pitarque ◽

José Miguel Lloris-Cejalvo ◽

Luis Gil-Santos ◽

Dolores Cejalvo-Lapeña

Keyword(s):

Wound Healing ◽

Early Stage ◽

Periodic Acid ◽

Healing Process ◽

Degradation Process ◽

Treated Group ◽

Complete Healing ◽

Male Rats ◽

Periodic Acid Schiff ◽

Liver Injuries

Sealants and adhesives are used in the repair and preservation of damaged solid organs. This study examines the matrix metalloproteinases (MMP) activity in the healing of liver injuries treated with two biological adhesives (Tachosil® and GelitaSpon®) as well as that of a new elastic cyanoacrylate (Adhflex®). Methods. We induced in 90 male rats hepatic lesions using a Stiefel biopsy punch in the liver. Wound healing was assessed 2, 6, and 18 days after injury by quantifying MMP1, 2, 8, 9, and 13 tissue levels. The histopathological repair was evaluated by hematoxylin-eosin, Masson’s trichrome, and Periodic Acid Schiff (PAS) staining and CD31, CD68 immunohistochemical marker. The three sealants used supported complete healing of liver lesions. Both histopathology and MMP findings indicate that the degradation process of Adhflex® is slower and produces a strong inflammatory reaction at the beginning of healing. Results. All the MMPs measured disclosed higher values at an early stage of the healing process in animals treated with Adhflex® and Tachosil, the expression of for MMP2 and MMP9 being significantly higher in the Adhflex-treated group. Animals treated with Tachosil had significant higher values of MMP8 and MPP13 than the Adhflex-treated group. Animals treated with Adhflex® showed a maintained overexpression in all the MMPs tested even at the latest wound healing stages. Conclusion. Notably, this MMPs overexpression did not negatively influence the histological healing process of hepatic injuries. Given that all hepatic trauma injuries should be considered emergencies, any easy-to-use and rapid sealant, such as Adhflex®, could be considered a suitable treatment option.

Download Full-text

Matrix Metalloproteinase Expression during the Healing Process of Liver Injuries Treated with Different Biological Sealants and Adhesives

10.20944/preprints201804.0262.v1 ◽

2018 ◽

Author(s):

José M. Lloris-Carsí ◽

Carlos Barrios ◽

José M. Lloris-Cejalvo ◽

Mar Lloris-Salvi ◽

Dolores Cejalvo-Lapeña

Keyword(s):

Wound Healing ◽

Early Stage ◽

Periodic Acid ◽

Healing Process ◽

Degradation Process ◽

Treated Group ◽

Complete Healing ◽

Periodic Acid Schiff ◽

Liver Injuries ◽

Male Wistar Rats

Background. Adhesives and sealants can be used to repair and preserve solid damaged organs. This study explores the activity of different matrix metalloproteinases (MMP) during the healing of liver injuries treated with two biological adhesives (Tachosil and GelitaSpon) and a new synthetic elastic cyanoacrylate (Adhflex®). Methods. Liver traumatic injuries were experimentally induced in 90 male Wistar rats using a Stiefel biopsy punch in the liver. Wound healing was evaluated 2, 6, and 18 days after injury by determining MMP1, 2, 8, 9, and 13 expression. The histopathological repair was assessed by hematoxylin-eosin, Masson’s trichrome, and Periodic Acid Schiff (PAS) staining. The three sealants used supported complete healing of the liver lesions. Both histopathology and MMP findings indicate that the degradation process of Adhflex® is slower and produces a strong initial inflammatory reaction. Results. All the MMPs measured disclosed higher values at early stage of the healing process in animals treated with Adhflex® and Tachosil, being the expression of for MMP2 and MMP9 significantly higher in the Adhflex-treated group. Animals treated with Tachosil had significant higher values of MMP8 and MPP13 than the Adhflex-treated group. Animals treated with Adhflex® showed a maintained overexpression in all the MMPs tested even at the latest wound healing stages. Conclusion. Notably, this MMPs overexpression did not influence negatively the histological healing process of the hepatic injuries. Given that all hepatic trauma injuries should be considered emergencies, any easy-to-use and rapid sealant, such as Adhflex®, could be considered as a suitable treatment option.

Download Full-text

The adverse effect of long term intake of Monosodium Glutamate on kidney performance

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/880/1/012056 ◽

2021 ◽

Vol 880 (1) ◽

pp. 012056

Author(s):

Amer M. Hussin ◽

Ali A. Tala’a ◽

Safa Abdul Naser Fadhil ◽

Hamzah Abdulrahman Salman

Keyword(s):

Mesangial Cells ◽

Periodic Acid ◽

Monosodium Glutamate ◽

Environmental Pollutant ◽

Basement Membranes ◽

Male Rats ◽

Periodic Acid Schiff ◽

Histological Techniques ◽

Treatment Groups

Abstract Monosodium glutamate (MSG) is a food additive that is considered as a water and environmental pollutant and affects the tissues of the living being. This study was aimed to find the effect of long-term administration of MSG on the mass of mesangial cells of the kidneys. Forty adult male rats were divided into four groups (10 each). Control groups 1&2 were supplied orally with distilled water for 30 and 60 days, respectively. Treatment groups 1&2 were supplied orally with 15 mg/kg Bwt of MSG for 30 & 60 days, respectively. Control and treatment groups were sacrificed, specimens of kidneys were obtained, fixed with 10% neutral buffered formalin, processed by Routine histological techniques, stained by Hematoxylin and eosin, and PAS (Periodic Acid-Schiff) stains then examined under the light microscope. The result found enlargement in a mesangial mass represented by hypertrophy and hyperplasia of mesangial cells leading to mesangial proliferative glomerulonephritis. Accordingly, the study showed an increase in creatinine values, indicating a disturbance in renal function. This will lead to a decrease in the sizes of the glomeruli of renal corpuscles and a relative increase of Bowman’s space. With the time of the experiment, the glomerular capillaries and gates of basement membranes will be closed, resulting in renal filtration disorders. It was concluded that the long-term intake of MSG leads to indirect narrowing of the glomerular capillary lumen, causing kidney failure.

Download Full-text

Histologic and inflammatory lamellar changes in horses with oligofructose-induced laminitis treated with a CXCR1/2 antagonist

Pesquisa Veterinária Brasileira ◽

10.1590/s0100-736x2016000100002 ◽

2016 ◽

Vol 36 (1) ◽

pp. 13-18 ◽

Cited By ~ 1

Author(s):

Leonardo R. de Lima ◽

Heloisa M.F. Mendes ◽

Frederico M. Soriani ◽

Danielle G. de Souza ◽

Geraldo Eleno S. Alves ◽

...

Keyword(s):

Chemokine Receptors ◽

Periodic Acid ◽

Previous History ◽

Basal Membrane ◽

Treated Group ◽

Periodic Acid Schiff ◽

Chemokine Signaling ◽

Mixed Breed ◽

History Of ◽

Membrane Changes

Abstract: With the hypothesis that blocking chemokine signaling can ameliorate acute laminitis, the aim was to evaluate the therapeutic effect of intravenous DF1681B, a selective antagonist for CXCR1 and CXCR2 (chemokine receptors), in an oligofructose equine laminitis model. To twelve mixed breed clinically healthy hoses with no previous history of hoof-related lameness was administered oligofructose (10g/kg given by nasogastric tube) and divided into two groups: treated (intravenous DF1681B at 30mg/kg 6, 12, 18, and 24h after oligofructose) and non-treated groups. Laminar biopsies were performed before and 12, 36, and 72h after administering oligofructose. Samples were stained with periodic acid-Schiff (PAS) and scored from 0 to 6 according to epidermal cell and basal membrane changes. The IL-1β, IL-6, and CXCL1 RNA expressions were determined by RT-PCR. Parametric and non-parametric tests were used to compare times within each group (P<0.05). The PAS grades and IL-1β and IL-6 RNA expression increased in the non-treated group, but remained constant in the treated horses. In conclusion, DF1681B therapy reduced laminar inflammation and epidermal deterioration in treated horses. CXCR1/2 blockage should be considered therapeutically for equine acute laminitis.

Download Full-text

Wound healing effect benzosulfonate 1-ethyl-3-methyl-4,5-bis(n-methylcarbamoyl) imidazolium

Reviews on Clinical Pharmacology and Drug Therapy ◽

10.17816/rcf183229-235 ◽

2020 ◽

Vol 18 (3) ◽

pp. 229-235

Author(s):

Lydmila K. Khnychenko ◽

Elena N. Selina ◽

Olga M. Rodionova ◽

Levon B. Piotrovskiy ◽

Petr D. Shabanov

Keyword(s):

Wound Healing ◽

Healing Process ◽

Experimental Models ◽

Skin Wound ◽

Complete Healing ◽

Male Rats ◽

Skin Wounds ◽

Skin Wound Healing ◽

Healing Effect ◽

Wound Healing Effect

Materials and methods. In experiments on 180 male rats weighing 180200 g, the wound-healing effect of derivatives of imidazole (IEM-1181) was evaluated as a 10% ointment on models of aseptic full-layer linear and planar skin wounds. Results. It was found that the compound IEM-1181 in the form of 10% ointment has a pronounced on skin wound healing effect, manifested in the qualitative features of the regenerative healing process. With local application of the ointment containing the tested compound, the strength of the tissue formed on the site of a full-layer linear skin wound was increased when its edges were stretched, and the healing time of full-layer planar wounds was reduced. The morphological picture of the tissue formed at the site of the wound defect corresponded to complete healing by primary tension with complete epithelization of the surface and squamous cell differentiation of the epithelial regenerate without signs of inflammation and scar formation. Conclusion. The results of the study on experimental models of full-layer linear and planar skin wounds indicate that the wound healing effect of the tested ointment is due to the anti-inflammatory activity of the IEM-1181 compound

Download Full-text

Hystometric evaluation of nickel chronic exposure effects on large instestine of adult Wistar male rats

Revista de Ciencias Agrícolas ◽

10.22267/rcia.1936e.103 ◽

2019 ◽

Vol 36 (E) ◽

pp. 21-30

Author(s):

Maria Luiza Assis Rodrigues ◽

Sirlene Souza Rodrigues Sartori ◽

Priscila Izabel Santos Totaro ◽

Sérgio Luis Pinto da Matta

Keyword(s):

Intestinal Mucosa ◽

Periodic Acid ◽

Exposure Period ◽

Nickel Chloride ◽

Male Rats ◽

Ascending Colon ◽

Periodic Acid Schiff ◽

Functional Aspects ◽

Acid Mucins ◽

Wistar Male Rats

The ingestion of considerable amounts of water or food contaminated with nickel can be very toxic. The present work was conducted aiming to evaluate the effects of nickel exposures on ascending colon of adult Wistar male rats at hystometric level. We used 12 animals that were divided in a control (ingested uncontaminated water) and a nickel-contaminated (i.e., 25 mg de nickel/L of water) groups. Nickel chloride was offered in declorinated water and the experiment had a 56 days exposure period. A portion of the ascending colon was removed of the animals and subjected to hystological labelling processes using blue toluidin (for general hystometric description), Alcian Blue (AB, for acid mucins) and periodic acid-Schiff (PAS) technique (for neutral mucins). The potential differences between groups were desgined by applying the Whitney test and t test (p < 0.05). The crypts were smaller for the nickel-contaminated group, even though these organism exhibited broader and higher crypts. Nickel-contaminated animals exhibited a smaller amount of calyceform cells with AB and PAS positive reactions as well as a less mucus quantities when compared with nickel-uncontaminated animals. Such reductions on the amount of calyceform cells with AB and PAS positive reactions may be related wiht the shallower crypts, which possibly reduced the synthesis and secretion of mucins, compromissing the functional aspects (e.g., lubrification and intestinal mucosa protection) of the nickel-contaminated large intestines. Interestingly, the wider and higher crypts and higher epithelium collumn on the nickel-contaminated animals may represent a relevant trade-off for the intestinal mucosa protection.

Download Full-text

Impact of Glyphosate-Roundup® in the Ileal Structure of Male and Female Rats: A Morphological and Immunohistochemical Study

Microscopy and Microanalysis ◽

10.1017/s1431927621012782 ◽

2021 ◽

pp. 1-17

Author(s):

Shaimaa M.M. Saleh ◽

Tasneem A. Elghareeb ◽

Mona M. Atia ◽

Mohamed Ahmed Ibrahim Ahmed

Keyword(s):

Periodic Acid ◽

Goblet Cells ◽

Female Rats ◽

Male Rats ◽

Electron Microscopic ◽

Periodic Acid Schiff ◽

Male And Female Rats ◽

Leukocytic Infiltration ◽

Strong Positive Reaction ◽

Metalloproteinase 9

Abstract The current study was aimed to evaluate the effects of variable doses of the weedicide glyphosate on the ileal (the final section of the small intestine) structure of rats of both sexes, using histological, histochemical, and ultrastructural methods. Forty animals were classified into four groups of 10 animals per group (five males and five females). The first group acted as a control, and the remaining groups were treated with glyphosate-Roundup® 25, 50, and 100 mg/kg body weight daily for 15 days. The results indicated extinct histopathological changes manifested in the deformation of villi, foci of leukocytic infiltration in the core of villi, and hyperplasia of goblet cells. Histochemical examination (Alcian blue and Periodic acid–Schiff stain) revealed a strong positive reaction of goblet cells and an increase in their number in all treated groups. In addition, the immunohistochemical investigation revealed the immunoreactivity of matrix metalloproteinase-9 expression. Furthermore, electron microscopic alternations were represented by the deformation of nuclei, destruction of microvilli, and deposition of lipid droplets. Collectively, the present findings indicate that treatment with glyphosate results in extensive morphological alternations to the ileal structure of rats of both sexes and that female rats are more affected than male rats are.

Download Full-text

Metal Induced Risk of Diabetes Mellitus Due to Toxicological Effects of Mercury: Influence of Environmental Threats

Current World Environment ◽

10.12944/cwe.15.3.11 ◽

2020 ◽

Vol 15 (3) ◽

pp. 470-476

Author(s):

Srikanta Guria

Keyword(s):

Diabetes Mellitus ◽

Pancreatic Islets ◽

Periodic Acid ◽

Treated Group ◽

Tolerance Test ◽

Periodic Acid Schiff ◽

Environmental Threats ◽

Toxicological Effects ◽

Glucose Levels ◽

Membrane Rupture

Diabetes Mellitus (DM) is a condition of hyperglycemia due to defects of insulin secretion and/or insulin action. Toxic metals such as lead, nickel, cadmium, arsenic and mercury have been identified which accumulate in various biological samples from T2D (type 2 diabetes) patients through environmental pollution and food chain. Present study will elucidate the toxicological effects of mercury (II) chloride in the pancreatic islets and liver tissues of rat which leads to dysfunction and degeneration of pancreatic islets and liver. Photomicrograph of histology of treated pancreas exhibited the disruption of islets, disorientation of cells and disruption of connective tissue septa. In mercury (II) chloride treated group pancreatic cells were found to be pyknotic and cellular death was confirmed by membrane rupture and necrosis. Alteration of blood glucose levels were observed by glucose tolerance test. The liver sections of rats treated with mercury (II) chloride showed modification in the structure of this organ. Treated liver showed lower periodic acid/Schiff response. In this study, changes in the architecture of pancreatic islets as well as liver may be the reason behind diabetes.

Download Full-text

Evaluation of the Effects of Photobiomodulation of Low-Level Laser on Total Thickness Burn Induced in Animal Model

Biointerface Research in Applied Chemistry ◽

10.33263/briac122.25172534 ◽

2021 ◽

Vol 12 (2) ◽

pp. 2517-2534

Keyword(s):

Animal Model ◽

Low Power ◽

Wound Repair ◽

Burn Injury ◽

Healing Process ◽

Treated Group ◽

Control Group ◽

Histopathological Analysis ◽

Power Laser ◽

Post Injury

Aim study was to evaluate the effects of low-power laser photobiomodulation (LBP) in third-degree burns induced in an animal model. Forty Wistar rats (Ratus norvegicus albinus) were used, females weighing between 200 and 300 grams, randomly divided into a control group (GC = 20) and a laser-treated group (DL = 20). A 1cm² third-degree burn injury was performed on the back of each animal after trichotomy and followed by surgical debridement 24 hours after induction. In the treated group (LBP) a laser was applied at a wavelength of 660 nm, a power of 27 mW and an average irradiance of 0.954 W / cm2 at five points of the wound. The total fluency was 350 Joules / cm2 and application of 5 Joules per wound. Biopsy fragments were collected on days 3, 7, 15 and 21 post-injury for macro and microscopic analysis using the techniques of Hematoxylin and Eosin (HE), Gomori's Trichrome (TG) and Picrosirius red. The results were evaluated by Tukey's Test and Analysis of Variance (ANOVA), Kruskal-Wallis Test (non-parametric) and T-paired Test. The healing process evaluated by morphometry showed a relative statistical difference between the CG and LBP groups. Histopathological analysis showed a decrease in the inflammatory infiltrate, angiogenesis, synthesis of granulation tissue and collagen deposition, increased fibroblasts, matrix organization, and reepithelization of the lesion. Thus, low-power laser photobiomodulation can contribute to the wound repair process efficiently.

Download Full-text