scholarly journals Study in vitro and in silico on effectiveness noni fruit extract (Morinda Citrifolia) to reducing hypertension

2020 ◽  
pp. 57-64
Author(s):  
La Ode Abdul Haris Hijriansyah ◽  
Hermilasari Hermilasari ◽  
Hardyanty Subair ◽  
Irianto Irianto ◽  
Andi Alief Utama Armyn ◽  
...  
Keyword(s):  
Blood Pressure ◽  
In Silico ◽  
Reduce Blood Pressure ◽  
Morinda Citrifolia ◽  
Control Group ◽  
In Vitro Test ◽  
Fruit Extract ◽  
Average Decrease ◽  
Vitro Test

Hypertension is one of the major causes of stroke. Stroke can be prevented by controlling hypertension. Noni fruit proved to have antihypertensive effect. Noni Fruit contains scopoletin and xeronin compounds that play a role in antihypertensives. This study aims to determine the effectiveness of noni fruit extract to controlling hypertension. The research method used is pre-test and post-test matched control group. The 6 Wistar rats were divided into 3 groups consisting of 1 treatment group and 2 control groups. Group P1 was induced by using ketamine 0.05 ml + epinephrine 0.2 ml + 6 ml noni fruit extract, group K (-) induced by ketamine 0.05 ml + epinephrine 0.2 ml without extract, and group K (+) induced by using ketamine 0.05 ml + epinephrine 0.2 ml + captopril 2.5 mg. The results showed that epinephrine can be used as a hypertensive inducer. Noni fruit extract as much as 6ml can provide antihypertensive effects. In Vitro, test result showed that noni fruit extract can reduce blood pressure by an average decrease in blood pressure of 58,5 mmHg While captopril 25,5 mmHg. in addition to the in vitro test, the results of the in-silico test showed that the noni fruit extract can significantly reduce blood pressure compared to anti-hypertensive drugs (captopril). the value of scopoletin in noni fruit is -7.6. and captopril only -5.7.

scholarly journals EST-based in silico identification and in vitro test of antimicrobial peptides in Brassica napus

BMC Genomics ◽  
2015 ◽  
Vol 16 (1) ◽  
Author(s):  
Tao Ke ◽  
Huihui Cao ◽  
Junyan Huang ◽  
Fan Hu ◽  
Jin Huang ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Antimicrobial Peptides ◽  
In Silico ◽  
In Vitro Test ◽  
Vitro Test ◽  
In Silico Identification

Establishment of correlation between in-silico and in-vitro test analysis against Leishmania HGPRT to inhibitors

2016 ◽  
Vol 83 ◽  
pp. 78-96 ◽  
Author(s):  
Md Yousuf Ansari ◽  
Asif Equbal ◽  
Manas Ranjan Dikhit ◽  
Rani Mansuri ◽  
Sindhuprava Rana ◽  
...  
Keyword(s):  
In Silico ◽  
In Vitro Test ◽  
Test Analysis ◽  
Vitro Test

scholarly journals Cytotoxic and genotoxic effects of the ent-kaurenoic acid and ent-kaurenoic acid enriched Mikania glomerata extract in V79

2020 ◽  
Vol 18 (1) ◽  
Author(s):  
Natália Ferreira ◽  
Arthur Ribeiro ◽  
Mariângela Morais ◽  
Aline Peixoto ◽  
Marcela Bernardino ◽  
...  
Keyword(s):  
Test System ◽  
Control Group ◽  
In Vitro Test ◽  
Genotoxic Effects ◽  
V79 Cells ◽  
Kaurenoic Acid ◽  
Biological Potential ◽  
Vitro Test ◽  
Mikania Glomerata

Abstract. Ferreira NH, Ribeiro AB, Morais MD, Peixoto AM, Bernardino MA, Moreira MR, Soares ACF, Heleno VCG, Veneziani RCS, Tavares DC. 2018. Cytotoxic and genotoxic effects of the ent-kaurenoic acid and ent-kaurenoic acid-enriched Mikania glomerata extract in V79. Biofarmasi J Nat Prod Biochem 17: xxxx. The ent-kaurenoic acid-rich extract from Mikania glomerata Sprengel was effective to inhibit the formation of Streptococcus mutans biofilm. In view of the biological potential of this extract and its major component, the present study was carried out to evaluate the safety of the ent-kaurenoic acid-rich extract from Mikania glomerata Sprengel and ent-kaurenoic acid alone in an in vitro test system. The results showed that the ent-kaurenoic acid-rich extract from Mikania glomerata Sprengel was cytotoxic at concentrations up to 40.0 μg/mL. Genotoxic effects were observed in cell cultures treated with the highest concentrations tested of ent-kaurenoic acid-rich extract from Mikania glomerata Sprengel (10.0 and 15.0 µg/mL) and ent-kaurenoic acid alone (2.5, 5.0 and 7.5 µg/mL) when compared to the control group. Therefore, the ent-kaurenoic acid-rich extract from Mikania glomerata Sprengel demonstrated cytotoxicity and genotoxicity effects at the highest concentrations tested, while ent-kaurenoic acid showed to be genotoxic at the same concentrations present in ent-kaurenoic acid-rich extract from Mikania glomerata Sprengel in V79 cells. These results demonstrate that the ent-kaurenoic acid should be responsible, at least in part, of the genotoxicity of ent-kaurenoic acid-rich extract from Mikania glomerata Sprengel.

scholarly journals Computer-Aided Drug Repurposing: A Cyclooxygenase-2 Inhibitor Celecoxib as a Ligand for Estrogen Receptor Alpha

2015 ◽  
Vol 15 (3) ◽  
pp. 274-280 ◽  
Author(s):  
Enade Perdana Istyastono ◽  
Florentinus Dika Octa Riswanto ◽  
Sri Hartati Yuliani
Keyword(s):  
Breast Cancer ◽  
Estrogen Receptor ◽  
In Silico ◽  
Estrogen Receptor Alpha ◽  
Cyclooxygenase 2 ◽  
Cytotoxic Activities ◽  
In Vitro Test ◽  
Receptor Alpha ◽  
Vitro Test

A cyclooxygenase-2 (COX-2) inhibitor celecoxib has been previously reported to have cytotoxic activities towards gastric, prostate, ovarian, colon and breast cancer cell lines. This article reports that the cytotoxic activities of celecoxib could be resulted from its activity as a potent ligand for estrogen receptor alpha (ERα). Aided by molecular docking simulations, an in silico test to examine whether celecoxib is a ligand for estrogen receptor alpha (ERα) was performed followed by in vitro test employing cytotoxic assay using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) method. The compound was extracted from Celebrex®. Measured by using UV spectrophotometric method at 255.5 nm, it was identified that the content of celecoxib was 102.15 mg/271.48 mg capsule content. The in silico test indicated that celecoxib is a potent ligand for ERα. This finding was confirmed experimentally by an in vitro test that celecoxib has a comparable activity as an ERα ligand to tamoxifen, a drug of choice for breast cancer treatment.

scholarly journals Selection of probiotic bifidobacteria for lambs

2009 ◽  
Vol 54 (No. 12) ◽  
pp. 552-565 ◽  
Author(s):  
E. Vlková ◽  
M. Grmanová ◽  
V. Rada ◽  
I. Homutová ◽  
S. Dubná
Keyword(s):  
Antimicrobial Activities ◽  
Control Group ◽  
In Vitro Test ◽  
Faecal Samples ◽  
Probiotic Treatment ◽  
Wild Strains ◽  
Vitro Test ◽  
Gradient Plate ◽  
Selection Of

Twenty-six bifidobacteria were isolated from faecal samples of lambs. The isolates were identified, functional properties (survival ability at low pH and bile conditions) and antimicrobial activities against potential pathogens were determined. From the isolates with suitable properties (13 strains) rifampicin-resistant mutants were prepared by gradient plate techniques. This property enabled us to differentiate the administered organism from wild strains because resistance to rifampicin is rare among bifidobacteria. Rifampicin-resistant bifidobacteria (RRBifs) were administered to 3-days-old lambs in two trials. In the first trial the strain <i>B. ruminantium</i> L29 was applied to 3 lambs and was detected in faecal samples at high counts (6 log CFU/g on average) for one week. In the second trial 3 lambs received a “cocktail” of 12 strains and RRBifs survived in the intestinal tract at counts of about 6 log CFU/g for 25 days. The control group without probiotic treatment consisted of 6 animals. In both treated groups RRBifs dominated among bifidobacteria after their administration. Total bifidobacterial counts (5.64–7.32 log CFU/g) were significantly higher (<i>P</i> < 0.05) in treated groups compared to 2.31–2.85 log CFU/g detected in the control group during the first month of lamb life. Lactobacilli counts were also significantly higher (<i>P</i> < 0.05) in treated groups compared to the control. The administered bifidobacteria did not affect any other monitored bacterial groups. On the basis of in vitro test results, suitable probiotic bifidobacterial strains for lambs were chosen. Some of them survived for 30 days in the gastrointestinal tract of treated lambs, but no tested strain was able to colonise the lamb’s tract permanently. The administration of bifidobacterial “cocktail” and consequent identification of the best survived strain seems to be an effective method for selection of potential probiotics.

scholarly journals A Practical Perspective for a Conservative Estimate of Blood Glucose Level during Restaurant Dining and Supermarket Shopping

Foods ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 444
Author(s):  
Xiao Chen ◽  
Peng Wu
Keyword(s):  
Blood Glucose ◽  
In Silico ◽  
Blood Glucose Concentration ◽  
Glycemic Load ◽  
Life Quality ◽  
Postprandial Blood Glucose ◽  
In Vitro Test ◽  
Conservative Estimate ◽  
Vitro Test

Foods today are so diverse and enjoyable, making healthy choices difficult. In this perspective, an in vitro-in silico approach for obtaining a conservative estimate of the postprandial blood glucose concentration, which is a realistic estimate nevertheless, after intake of a certain portion of meals is proposed. The rationales and feasibilities of the approach are described and discussed to an extent. The key idea is to first measure the maximum amount of glucose released in an in vitro test under standardized conditions from a specified serving size of a meal or dish or a packaged product sold in a supermarket. The value can then be translated by a literate consumer to the highest estimate of blood glucose rise prior to purchasing or eating through an established in silico blood glucose prediction model in the medical field. The strategy proposed here would help health conscious (diabetics included) and other life quality conscious individuals to make quantitative decisions on consuming the portions of different foods of desire. This strategy may be more effective in reality compared to the conventional GI (Glycemic Index) and GL (Glycemic Load) concepts.

LITERATUR REVIEW : EFEKTIVITAS BUAH MENGKUDU (Morinda Citrifolia L.) TERHADAP PENURUNAN HIPERTENSI

2021 ◽  
Vol 13 (02) ◽  
pp. 128
Author(s):  
Roikatul Miskiyah ◽  
Friska Realita
Keyword(s):  
Blood Pressure ◽  
Treatment Group ◽  
Systolic Blood Pressure ◽  
Statistical Test ◽  
Morinda Citrifolia ◽  
Control Group ◽  
P Value ◽  
Fruit Extract ◽  
Before And After ◽  
Interaction Factors

ABSTRAKLatar Belakang : Hipertensi adalah The Silent Disease sehingga seseorang tidak akan mengetahui jika dirinya menderita hipertensi atau tekanan darah tinggi. Meningkatnya tekanan darah sistolik lebih besar dari 120 mmHg dan diastolik lebih besar dari 80 mmHg pada dua kali pengukuran dengan selang waktu 5 menit dalam keadaan cukup istirahat disebut dengan Hipertensi, Hal tersebut timbul karena adanya banyak faktor interaksi resiko yang dimiliki seseorang. Tujuan : Penelitian ini bertujuan untuk menganalisis efektivitas buah mengkudu dalam menurunkan hipertensi. Metode : Pencarian artikel nasional ini dilakukan dengan basis data seperti google scohlar. Dengan kata kunci: “hipertensi”.”ekstrak buah mengkudu”. Dan penulis menemukan 7 artikel yang relevan 2014-2018. masing-masing mewakili efektivitas buah mengkudu terhadap penurunan hipertensi terhadap semua sampel.  Hasil  : Tujuh artikel yang di dapat, Menunjukkan hasil uji statistik  p Value 0,015 (p<0,05). berarti ada pengaruh antara tekanan darah siastolik maupun diastolik penderita hipertensi sebelum dan sesudah diberikan air perasan buah mengkudu. Kesimpulan : tekanan darah pada kelompok perlakuan sesudah intervensi buah mengkudu mengalami penurunan tekanan darah dibandingkan dengan kelompok control, efektivitas buah mengkudu ialah salah satu pengobatan non farmakologis.Kata kunci: Hipertensi, Ekstrak Buah MengkuduTHE EFFECTIVENESS OF NONI FRUIT (MORINDA CITRIFOLIA L.)    TO REDUCE HYPERTENSIONABSTRACTBackground : Hypertension is The Silent Disease so that a person will not know if he has hypertension or high blood pressure. Increased systolic blood pressure greater than 120 mmHg and diastolic greater than 80 mmHg in two measurements with an interval of 5 minutes in a state of sufficient rest is called Hypertension. This arises because there are many risk interaction factors that a person has. Objective : This study aims to analyze the effectiveness of noni fruit in reducing hypertension. Methods : This national article search was carried out using databases such as Google Scohlar. With keywords: "hypertension", "noni fruit extract". And the author found 7 relevant articles 2014-2018. each represents the effectiveness of noni fruit in reducing hypertension in all samples. Results : Seven articles were obtained, showing the results of the statistical test p Value of 0.015 (p <0.05). This means that there is an influence between systolic and diastolic blood pressure in patients with hypertension before and after being given noni juice. Conclusion : blood pressure in the treatment group after the noni fruit intervention decreased blood pressure compared to the control group, the effectiveness of noni fruit is one of the non-pharmacological treatments.Keywords: Hypertension, Mengkudu Fruit Extract.

scholarly journals THE EVALUATION OF THERMAL PROPERTIES AND IN VITRO TEST OF CARBODIIMIDE OR GLUTARALDEHYDE CROSS-LINKED GELATIN FOR PC 12 CELLS CULTURE

2005 ◽  
Vol 17 (02) ◽  
pp. 101-107 ◽  
Author(s):  
PEI-RU CHEN ◽  
PEI-LEUN KANG ◽  
WEN-YU SU ◽  
FENG-HUEI LIN ◽  
MING-HONG CHEN
Keyword(s):  
Covalent Binding ◽  
Cell Activity ◽  
Endothermic Peak ◽  
Cross Linking ◽  
Control Group ◽  
In Vitro Test ◽  
Denaturation Temperature ◽  
Vitro Test ◽  
The Stability

The thermal and degradable properties of carbodiimide (EDC) or glutaraldehyde (GTA) cross-linked gelatin membranes have been investigated in order to evaluate the effects of different concentrations of two kinds of cross-linking reagent on the stability of membranes. In the thermogram recorded from a gelatin membrane cross-linked with EDC solution, the endothermic peak of 0.8% EDC cross-linking gelatin was centered at about 61°C that was higher than other samples treated with EDC solutions. Denaturation temperature (Td) of gelatin samples increased on increasing EDC concentration (0.2% to 0.8%), in agreement with the simultaneous increased of the extent of cross-linking. But increasing GTA concentration from 0.05% to 0.6%, the Td values of gelatin samples were decreased from 66.2°C to 56.3°C . In addition, two endothermic peaks were observed in 0.4% and 0.6% GTA cross-linking groups because of the GTA concentration was too high to complete cross-linking reaction. Therefore, partial of gelatin membrane was cross-linked completely but others were not. In the thermogravimetric analysis, the proportion of cracking endothermic peak of 0.6% GTA cross-linking gelatin (g15G0.6) was higher than the peak of 0.6% EDC cross-linking gelatin (g15C0.6). Therefore, g15G0.6 cracked to smaller molecules has to absorb more calorific capacity than g15C0.6. The increase in the strength of covalent binding on increasing the proportion of endothermic peak was evident. The results of degradable rate were in agreement with the lower concentration of cross-linked reagent the faster degraded rate of gelatin membrane. The MTT assay showed that 15% gelatin cross-linked by 0.8% EDC has the least cytotoxicity, and cell activity of this group was similar to control group (blank dish). As the concentration of GTA in gelatin membranes was down to 0.05% or 0.1% the cell viability was returned to approach the value of control group.

In Silico Study and In Vitro test of Extract Kaempferia pandurata Roxb. as Anti ER (+) Breast Cancer Cell Line MCF-7

2019 ◽  
Vol 12 (5) ◽  
pp. 2391
Author(s):  
Fadilah Fadilah ◽  
Lowilius Wiyono ◽  
Brenda Cristie Edina ◽  
Risya Amelia Rahmawati ◽  
Linda Erlina ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Line ◽  
Breast Cancer Cell Line ◽  
In Silico ◽  
Cancer Cell Line ◽  
In Vitro Test ◽  
In Silico Study ◽  
Vitro Test ◽  
Mcf 7

scholarly journals Cocoa (Theobroma cacao L.) Seed-Derived Peptides Reduce Blood Pressure by Interacting with the Catalytic Site of the Angiotensin-Converting Enzyme

Foods ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 2340
Author(s):  
Luis Jorge Coronado-Cáceres ◽  
Blanca Hernández-Ledesma ◽  
Luis Mojica ◽  
Lucía Quevedo-Corona ◽  
Griselda Rabadán-Chávez ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Angiotensin Converting Enzyme ◽  
In Silico ◽  
Reduce Blood Pressure ◽  
Converting Enzyme ◽  
Ic50 Value ◽  
Theobroma Cacao L

This study aimed at determining the effect of cocoa proteins (CP) on the blood pressure, using in silico, in vitro and in vivo approaches. The in silico assay showed 26 Criollo cocoa peptides with alignment in the Blast® analysis. Peptide sequences ranged from 6 to 16 amino acids, with molecular weight ranging from 560.31 to 1548.76 Da. The peptide sequences LSPGGAAV, TSVSGAGGPGAGR, and TLGNPAAAGPF showed the highest theoretical affinity with −8.6, −5.0, and −10.2 kcal/mol, for the angiotensin-converting enzyme (ACE), renin, and angiotensin II type 1 receptor (AT1-R), respectively. The Criollo CP hydrolysates (CPH) presented in vitro ACE inhibitory activity with an IC50 value of 0.49 mg/mL. Furthermore, the orogastric administration of 150 mg CP/kg/day in rats fed a high-fat (HF) diet (HF + CP group) showed a significant decrease in systolic blood pressure (SBP) by 5% (p < 0.001) and diastolic blood pressure (DBP) by 7% (p < 0.001) compared with the HF group. The human equivalent dose (HED) of CP for an adult (60 kg) is 1.45 g per day. These results suggest that the consumption of CP could reduce blood pressure by blocking ACE, and could be used as an ingredient in the elaboration of antihypertensive functional foods.

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