ANTI-PROLIFERATIVE ACTIVITIES OF THE AQUEOUS ROOT EXTRACT OF DIANTHUS THUNBERGII SS HOOPER (CARYOPHYLLACEAE)

2018 ◽  
Vol 15 (4) ◽  
pp. 32 ◽  
Author(s):  
Akinleye Akinrinde ◽  
Maryna Van de Venter ◽  
Trevor Koekemoer ◽  
Graeme Bradley
Keyword(s):  
Cell Lines ◽  
Cancer Cell ◽  
Aqueous Extract ◽  
Cancer Cell Lines ◽  
Root Extract ◽  
Diphenyltetrazolium Bromide ◽  
Ethanol Extracts ◽  
Cellular Carcinoma ◽  
Aqueous Root Extract

Background: The roots of Dianthus thunbergii SS Hooper are used traditionally in South Africa for the treatment of diabetes, wounds, colic, chest complaints and cancer. This study was aimed at investigating the potential anti-proliferative activities of the D. thunbergii in mammalian cancer cell lines. Materials and Methods: Aqueous and ethanol extracts of D. thunbergii were tested in vitro on two cancer cell lines: human hepato-cellular carcinoma (HepG2) cells and murine insulinoma (INS-1) cells using the 3-(4,5-Dimethylthiazol-2- yl) 2,5-diphenyltetrazolium bromide (MTT) and crystal violet cell viability assays, as well as live-cell fluorescence imaging microscopy. A tentative profiling of the aqueous extract was also carried out using liquid chromatography-mass spectrometry (LC-MS). Results: The aqueous extract (50-200μg/ml) exhibited significant (p

scholarly journals An In Vitro Estimation of the Cytotoxicity and Genotoxicity of Root Extract from Leonurus sibiricus L. Overexpressing AtPAP1 against Different Cancer Cell Lines

Molecules ◽  
2018 ◽  
Vol 23 (8) ◽  
pp. 2049 ◽  
Author(s):  
Przemysław Sitarek ◽  
Ewelina Synowiec ◽  
Tomasz Kowalczyk ◽  
Tomasz Śliwiński ◽  
Ewa Skała
Keyword(s):  
Cell Lines ◽  
Cancer Cell ◽  
Cytotoxic Effect ◽  
Treatment Success ◽  
Cancer Cell Lines ◽  
Nadh:Ubiquinone Oxidoreductase ◽  
Cytotoxic Activities ◽  
Root Extract ◽  
Root Extracts

As the current cancer treatment success rate is not sufficient, interest has grown in plants as possible sources of anti-cancer compounds. One such plant with a broad spectrum of activity is Lenourus sibiricus of the family Lamiaceae. This study investigates for the first time both the genotoxic and cytotoxic activities of TR (transformed) and AtPAP1 TR (with over-expression of transcriptional factor) root extracts of Lenourus sibiricus against various cancer cell lines (CCRF-CEM, K-562 and A549). Both tested extracts showed a cytotoxic effect on CCRF-CEM and K-562 cell lines, but strongest activity was observed for the AtPAP1 TR extract. No cytotoxic effect was observed against the A549 cell line in the tested concentration range, and it was found that both tested extracts may induce apoptosis by decreasing mitochondrial membrane potential and inducing nDNA damage lesion in the TP53 region and mtDNA in ND1 (mitochondrially encoded NADH: ubiquinone oxidoreductase core subunit 1) and ND5 (mitochondrially encoded NADH:ubiquinone oxidoreductase core subunit 5) regions in K-562 and CCRF-CEM. Our results confirmed that TR and AtPAP1 TR root extracts from L. sibiricus are cytotoxic and genotoxic against different model cell lines (CCRF-CEM and K-562). However, the observed genotoxicity of both extracts needs to be confirmed by additional studies. These preclinical observations support the use of L. sibiricus with other pharmacological purposes.

Cytotoxic activity of ethanol extracts of in vitro grown Cistus creticus subsp. creticus L. on human cancer cell lines

2012 ◽  
Vol 38 ◽  
pp. 153-159 ◽  
Author(s):  
Marijana Skorić ◽  
Slađana Todorović ◽  
Nevenka Gligorijević ◽  
Radmila Janković ◽  
Suzana Živković ◽  
...  
Keyword(s):  
Cytotoxic Activity ◽  
Cell Lines ◽  
Cancer Cell ◽  
Human Cancer ◽  
Cancer Cell Lines ◽  
Human Cancer Cell ◽  
Human Cancer Cell Lines ◽  
Ethanol Extracts ◽  
Cistus Creticus

In vitro Cytotoxic Activity of Vatica diospyroides Symington Type LS Root Extract on Breast Cancer Cell Lines MCF-7 and MDA-MB-468

2013 ◽  
Vol 13 (2) ◽  
pp. 130-135 ◽  
Author(s):  
Theera Srisawat ◽  
Parinuch Chumkaew ◽  
Warapond Maichum ◽  
Yaowapa Sukpondma ◽  
Potchanapond Graidist ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cytotoxic Activity ◽  
Cell Lines ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Cancer Cell Lines ◽  
Breast Cancer Cell Lines ◽  
Root Extract ◽  
Mcf 7

scholarly journals CYTOTOXICITY OF STEM EXTRACTS OF SELECTED CASSIA SPECIES AGAINST HELA AND BREAST CANCER CELL LINES IN VITRO

2017 ◽  
Vol 10 (3) ◽  
pp. 80
Author(s):  
Shankar B Naik ◽  
Surekha R Deshpande
Keyword(s):  
Breast Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Cancer Cell Lines ◽  
Maximum Activity ◽  
Breast Cancer Cell Lines ◽  
Soxhlet Apparatus ◽  
Diphenyltetrazolium Bromide

ABSTRACTObjective: The use of plants for medicinal remedies is an integral part of the Indian cultural life, and the traditional background of Indian medicineshows widespread use of plant products in cancer treatment. In this study, stem extracts of some selected Cassia species have been evaluated for theircytotoxic activities under in vitro conditions.Methods: The stems were shade dried at room temperature. The dried and coarsely powdered plant material were extracted with petroleum ether(60-80°C), chloroform, and ethanol using soxhlet apparatus. The cytotoxicity was evaluated by [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide] assay.Results: Stem extracts of three Cassia species, viz., Cassia glauca, Cassia Obtusifolia, and Cassia sophera have been evaluated for their cytotoxic activitieswith chloroform, ethanol and pet ether against HeLa and breast cancer cell lines. Among the three different solvents used at different concentrations,the chloroform extracts of all Cassia species exhibited maximum cytotoxicity (%) against both cell lines. The CTC50 values are revealed the cytotoxicpotential of C. glauca chloroform extracts against HeLa cell line and breast cancer cell lines with CTC50 values 180.00±3.0 and 146.67±0.5, respectively.Chloroform extracts of C. Obtusifolia and C. sophera showed maximum activity against HeLa (380.00±1.1 and 800.00±1.7, respectively) and breastcancer cell lines (310.00±1.1 and 633.33±0.6).Conclusion: The results of this study demonstrate the potent cytotoxic activity of chloroform extracts of stems of Cassia species against HeLa andBreast cancer cell lines.Keywords: Cassia, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, Cytotoxicity, HeLa, Breast cancer.

scholarly journals Evaluation of in vitro anti-cancer effects of Styphnolobium japonicum root extract in human colon (HT-9), brain (U-87), and prostate (PC-3) cancer cell lines

2020 ◽  
Vol 50 (2) ◽  
Author(s):  
Mehmet Evren Okur ◽  
Nihal Karakaş ◽  
Ayşe Esra Karadağ ◽  
Nurşah Öztunç ◽  
İbrahim Serkut Tosyalı ◽  
...  
Keyword(s):  
Cell Lines ◽  
Cancer Cell ◽  
Human Colon ◽  
Cancer Cell Lines ◽  
Root Extract ◽  
Anti Cancer

ANTITUMOR EFFECT OF SUNITINIB AND BORTEZOMIB ON BREAST CANCER CELL LINE IN VITRO

2017 ◽  
Vol 63 (1) ◽  
pp. 141-145
Author(s):  
Yuliya Khochenkova ◽  
Eliso Solomko ◽  
Oksana Ryabaya ◽  
Yevgeniya Stepanova ◽  
Dmitriy Khochenkov
Keyword(s):  
Breast Cancer ◽  
Cell Line ◽  
Cell Lines ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Antitumor Effect ◽  
Cancer Cell Lines ◽  
Breast Cancer Cell Lines ◽  
Actual Problem

The discovery for effective combinations of anticancer drugs for treatment for breast cancer is the actual problem in the experimental chemotherapy. In this paper we conducted a study of antitumor effect of the combination of sunitinib and bortezomib against MDA-MB-231 and SKBR-3 breast cancer cell lines in vitro. We found that bortezomib in non-toxic concentrations can potentiate the antitumor activity of sunitinib. MDA-MB-231 cell line has showed great sensitivity to the combination of bortezomib and sunitinib in vitro. Bortezomib and sunitinib caused reduced expression of receptor tyrosine kinases VEGFR1, VEGFR2, PDGFRa, PDGFRß and c-Kit on HER2- and HER2+ breast cancer cell lines

Small Molecular Leads Differentially Active Against HER2 Positive and Triple Negative Breast Cancer Cell Lines

2019 ◽  
Vol 15 (7) ◽  
pp. 738-742 ◽  
Author(s):  
Adnan Badran ◽  
Atia-tul-Wahab ◽  
Sharmeen Fayyaz ◽  
Elias Baydoun ◽  
Muhammad Iqbal Choudhary
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Cell Lines ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Triple Negative ◽  
Cancer Cell Lines ◽  
Breast Cancer Cell Lines ◽  
Cancer Type

Background:Breast cancer is the most prevalent cancer type in women globally. It is characterized by distinct subtypes depending on different gene expression patterns. Oncogene HER2 is expressed on the surface of cell and is responsible for cell growth regulation. Increase in HER2 receptor protein due to gene amplification, results in aggressive growth, and high metastasis in cancer cells.Methods:The current study evaluates and compares the anti-breast cancer effect of commercially available compounds against HER2 overexpressing BT-474, and triple negative MDA-MB-231 breast cancer cell lines.Results:Preliminary in vitro cell viability assays on these cell lines identified 6 lead molecules active against breast cancer. Convallatoxin (4), a steroidal lactone glycoside, showed the most potent activity with IC50 values of 0.63 ± 0.56, and 0.69 ± 0.59 µM against BT-474 and MDA-MB-231, respectively, whereas 4-[4-(Trifluoromethyl)-phenoxy] phenol (3) a phenol derivative, and Reserpine (5) an indole alkaloid selectively inhibited the growth of BT-474, and MDA-MB-231 breast cancer cells, respectively.Conclusion:These results exhibited the potential of small molecules in the treatment of HER2 amplified and triple negative breast cancers in vitro.

In Vitro Antitumor Evaluation of Some Hybrid Molecules Containing Coumarin and Quinolinone Moieties

2020 ◽  
Vol 19 (16) ◽  
pp. 2010-2018
Author(s):  
Youstina W. Rizzk ◽  
Ibrahim M. El-Deen ◽  
Faten Z. Mohammed ◽  
Moustafa S. Abdelhamid ◽  
Amgad I.M. Khedr
Keyword(s):  
Cell Cycle ◽  
Cell Lines ◽  
Cancer Cell ◽  
Anticancer Agents ◽  
Topoisomerase Ii ◽  
Cancer Cell Lines ◽  
Bax Protein ◽  
Hybrid Molecules ◽  
Mcf 7

Background: Hybrid molecules furnished by merging two or more pharmacophores is an emerging concept in the field of medicinal chemistry and drug discovery. Currently, coumarin hybrids have attracted the keen attention of researchers to discover their therapeutic capability against cancer. Objective: The present study aimed to evaluate the in vitro antitumor activity of a new series of hybrid molecules containing coumarin and quinolinone moieties 4 and 5 against four cancer cell lines. Materials and Methods: A new series of hybrid molecules containing coumarin and quinolinone moieties, 4a-c and 5a-c, were synthesized and screened for their cytotoxicity against prostate PC-3, breast MCF-7, colon HCT- 116 and liver HepG2 cancer cell lines as well as normal breast Hs-371 T. Results: All the synthesized compounds were assessed for their in vitro antiproliferative activity against four cancer cell lines and several compounds were found to be active. Further in vitro cell cycle study of compounds 4a and 5a revealed MCF-7 cells arrest at G2 /M phase of the cell cycle profile and induction apoptosis at pre-G1 phase. The apoptosis-inducing activity was evidenced by up-regulation of Bax protein together with the downregulation of the expression of Bcl-2 protein. The mechanism of cytotoxic activity of compounds 4a and 5a correlated to its topoisomerase II inhibitory activity. Conclusion: Hybrid molecules containing coumarin and quinolinone moieties represents a scaffold for further optimization to obtain promising anticancer agents.

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