Application of magnetic bead method in detecting coagulation function

Early screening is an important way to reduce the incidence and mortality of colorectal cancer (CRC). Fecal DNA testing stands out among many screening methods due to its high sensitivity. However, at this stage, researchers have not found a high-efficiency method for fecal DNA extraction. To this end, this work carried out a new round of exploration. Here, this experiment synthesized a kind of nanomagnetic beads (NH2-SiO2@Fe3O4) with good stability for nucleic acid extraction. A comparative study with the centrifugal adsorption column method revealed the significant advantages of the magnetic bead method in extracting fecal DNA. The DNA extracted by the magnetic bead method is of high purity, can also achieve high-throughput tests, and is more suitable for polymerase chain reaction detection, greatly simplifying the stool DNA detection process and providing a basis for the widespread promotion of early screening.

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DNA extraction and sequencing of the Mawangdui ancient cadaver protected by formalin

10.1101/2020.05.06.081711 ◽

2020 ◽

Author(s):

Hua-Lin Huang ◽

Shikui Yin ◽

Huifang Zhao ◽

Chao Tian ◽

Jufang Huang ◽

...

Keyword(s):

Dna Extraction ◽

High Throughput ◽

High Throughput Sequencing ◽

Pcr Amplification ◽

Magnetic Bead ◽

Sequencing Data ◽

Sequencing Library ◽

Bead Method ◽

Formalin Fixed ◽

Better Than

AbstractMawangdui ancient Cadaver is the first wet corpse found in the world, which is famous for being immortal for over two thousands of years. After being unearthed, the female corpse was immersed in the formalin protective solution for more than 40 years. We used magnetic bead method and formalin fixed paraffing (FFPE) method to extract the DNA of the female corpse, respectively. PCR amplification, sanger sequencing, library building, high throughput sequencing (testing) and data processing were carried out on the DNA samples, and about 0.5% of the whole genome coverage sequencing data was obtained. Comparing the results of DNA trough two extraction and sequencing methods. We found that the FFPE and high throughput sequencing methods is better than others for DNA extraction of the ancient samples which were preserved in formalin, providing a guidance for dealing with formalin preserved ancient samples in the future.

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A Phos-tag-based magnetic-bead method for rapid and selective separation of phosphorylated biomolecules

Journal of Chromatography B ◽

10.1016/j.jchromb.2013.02.039 ◽

2013 ◽

Vol 925 ◽

pp. 86-94 ◽

Cited By ~ 16

Author(s):

Masaya Tsunehiro ◽

Yuma Meki ◽

Kanako Matsuoka ◽

Emiko Kinoshita-Kikuta ◽

Eiji Kinoshita ◽

...

Keyword(s):

Magnetic Bead ◽

Selective Separation ◽

Bead Method

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The study on knee osteoarthritis in diabetic patients based on nanometric magnetic bead method

Materials Express ◽

10.1166/mex.2020.1815 ◽

2020 ◽

Vol 10 (8) ◽

pp. 1271-1277

Author(s):

Dongbin Luo ◽

Dabiao Hou ◽

Simin Luo

Keyword(s):

Inflammatory Cytokines ◽

Peripheral Blood ◽

Mononuclear Cells ◽

Interleukin 1 ◽

Magnetic Bead ◽

Inflammatory Factors ◽

Control Group ◽

Diabetic Patients ◽

Peripheral Blood Mononuclear ◽

Bead Method

To study the levels of inflammatory cytokines in synovia fluid from osteoarthritis (OA) patients with/without type 2 diabetes mellitus (T2D). Out of 74 volunteers aged 20–88 years, 64 with knee OA (Kellgren-Lawrence grades over I) were recruited and divided into groups with (n = 20) and without (n = 44) diabetes. The nucleic acid of the patient tissue was extracted by nanometer magnetic bead method. The remaining participants were the control group (n = 10). Synovia fluid, sera, and peripheral blood mononuclear cell (PBMC) samples were collected from all participants and analyzed using ELISA Kits. T2D-OA patients and OA patients had higher basal production of interleukin-1, (IL-1), interleukin-6 (IL-6), and lower-level interleukin-10 (IL-10). However, there were no differences between T2D-OA and OA patients. IL-1, IL-10, and IL-6 in peripheral blood mononuclear cells (PBMCs) after stimulation with LPS were obviously up-regulated in both patients and controls. The production of IL-1 and IL-6 in synovia from T2D-OA and OA patients increased as in the case of variations in sera and PBMCs. Both T2D-OA and OA patients had high-levels of IL-1 and IL-6 compared with controls, especially IL-6. The presence of IL-10 could not be detected in synovia from both patients and controls. Our results suggested that OA patient also had some pro-inflammatory factors in vivo, especially in local lesion; this manifest was particularly evident in T2D-OA patients. The high-level concentration of pro-inflammatory cytokines and low-level anti-inflammatory factors could be one of the reasons why T2D-OA patients are prone to developing synovitis.

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