scholarly journals Karakterisasi dan Evaluasi Aksesi Pepaya Introduksi

2016 ◽  
Vol 14 (2) ◽  
pp. 81
Author(s):  
NFN Sudjijo

<p>New varieties of papaya can be obtained from the exploration of germplasms, conventional breeding, genetic engineering and the introduction of new cultivars from overseas. This study was conducted at Sumani Experimental Field of the Indonesian Tropical Fruit Research Institute from April 2006 to March 2007. This study aimed at characterizing papaya accessions based on their morphological characters listed as descriptor published by IBPGR (1988). The plant materials used were 3 accessions, of papaya introduced from India, namely SR-01, SR-02, SR-03, and 1 local variety. From three papaya accessions observed, SR-03 produced ideal fruit size (840 g), sweet (13.21o Brix of total soluble solid), firm flesh texture, 25.20 mm of pulp thickness, orange to red flesh colour. This accession was promising for further development.</p><p> </p><p><strong>Abstrak</strong></p><p>Plasma nutfah merupakan salah satu subtansi sumber daya genetik yang dapat dimuliakan untuk merakit varietas baru. Sumber plasma nutfah dapat diperoleh melalui rekayasa genetika, eksplorasi maupun introduksi. Penelitian dilaksanakan di Kebun Percobaan Sumani, Balai Penelitian Tanaman Buah Tropika, menggunakan material benih tiga aksesi pepaya introduksi dari India, terdiri dari (SR-01, SR-02, SR-03) dan 1 varietas lokal, dimulai dari bulan April 2006-Maret 2007. Penelitian bertujuan untuk memperoleh data base karakter dari aksesi pepaya tersebut. Metode penelitian menggunakan Descriptor List for Papaya. Dari hasil pengamatan diperoleh data bahwa nomor aksesi SR-03 memiliki ukuran buah ideal, rasa manis, kenyal, dan berproduksi sepanjang tahun, bobot buah 840 g, tebal daging 25,2 mm, padatan total terlarut (PTT) 13,21o Brix, warna jingga.</p>

Author(s):  
Aline Sisi Handini ◽  
Dewi Sukma ◽  
Dan Sudarsono

ABSTRACT<br /><br />Phalaenopsis orchid is one of famous genera in Orchidaceae family which have high economic value. Phalaenopsis breeding needs to be developed intensively to fulfill market demand for new varieties. The objective of this study was to obtain the information of  morphological and biochemical diversity of a collection of Phalaenopsis orchid which will be useful for Phalaenopsis breeding. Plant materials used were 10 genotypes of Phalaenopsis with various petal colours of white, yellow, pink, deep pink, purple, or combination of yellow and brown. Morphological diversity of 10 genotypes were analyzed based on qualitative and quantitative characters following UPOV guidance for Phalaenopsis, while biochemical diversity was based on pigment content such as chlorophyl, anthocyanin, and carotene in plant roots, leaves and flower petals. The result of this research showed morphological diversity of 10 genotypes used was relatively high, with similarity of 55%. In that level of similarity, genotypes were grouped into two cluster. Biochemical analysis showed that most of genotypes were different for anthocyanin and carotenoid content for each plant organ. Genotype which showed high content of anthocyanin in leaves was Phal. hybrid ‘PH37’ while high content of carotene in leaves was Phal. hybrid ‘PIROUI. High content of anthocyanin in petals were found in Phal. hybrid ‘PH37’ and Phal. esmeralda, and high content of carotene were in Phal. cornucervi and Phal. cornucervi Red. Root with high anthocyanin content was found in Phal. hybrid ‘PIROU1’. There were significant correlation (0.975) for anthocyanin content between leaves and petals, petals and roots (0.953) on Phal.hybrid ‘PH37’, and between leaves and roots (0.874) on Phal. hybrid ‘PIROU1).<br /><br />Keywords: anthocyanin, carotene, chlorophyll, Phalaenopsis, similarity


2017 ◽  
Vol 11 (1) ◽  
pp. 97
Author(s):  
Ridla Arifriana ◽  
Sapto Indrioko ◽  
Atus Syahbudin

Cendana (Santalum album Linn.) merupakan salah satu spesies yang digunakan sebagai tanaman rehabilitasi, dikenal mampu tumbuh di lahan dengan keterbatasan hara, dan memiliki nilai ekonomi tinggi dari kandungan minyak santalol yang wangi. Cendana merupakan spesies asli di Provinsi Nusa Tenggara Timur (NTT) namun dijumpai permudaan alami di beberapa kawasan Kabupaten Gunungkidul, Daerah Istimewa Yogyakarta (DIY), salah satunya di Desa Petir, Kecamatan Rongkop, Kabupaten Gunungkidul. Permudaan alami tersebut memiliki variasi morfologi yang relatif besar pada populasinya. Penelitian ini bertujuan mendeskripsikan variasi cendana dan mengklasifikasikan cendana yang ditemui di lokasi penelitian berdasarkan karakter morfologi. Bahan yang digunakan dalam penelitian ini berupa (1) cendana (meliputi organ generatif dan vegetatif) yang diambil secara sengaja (purposive) dan (2) Ximenia americana sebagai spesies pembanding. Data dideskripsi secara morfologi dan dilakukan analisis filogeni menggunakan metode maksimum parsimoni dengan penanda morfologi. Karakter morfologi cendana bervariasi pada ukuran dan bentuk daun, warna tenda bunga (perigonium), bunga, bentuk cuping, bentuk appendiks, warna batang, bentuk dan ukuran buah. Ditemui dua karakter cendana yang belum dijelaskan pada sumber sebelumnya di Indonesia yaitu bentuk daun lanset dan bulat telur serta karakter warna tenda bunga (perigonium), bunga oranye. Hasil analisis filogeni menunjukkan karakter daun kecil cendana lebih dekat dengan yang dimiliki oleh spesies pembanding. Karakter diagnostik secara tegas mengelompokan ukuran daun kecil dan ukuran daun besar. Ukuran daun kecil pada kisaran 2,8-7,7 cm sampai dengan 1,1-2,2 cm dan 7,9-10 cm sampai dengan 3,1-4 cm untuk cendana berdaun besar. Variasi bentuk daun serta warna bunga ditemukan pada masing-masing kelompok ukuran daun.Variation of Sandalwood (Santalum album Linn.) Based on Morphology of Leaves and Flowers at Petir Village, Rongkop, GunungkidulAbstractSandalwood (Santalum album Linn.) is one of the flora that are commonly used as rehabilitation species, known as a plant that can grow in a lack of nutrient soil, and has a high economic value of santalol oil. This plant is indigenous in East Nusa Tenggara but it has a natural regenaration in some areas of Gunungkidul Yogyakarta, including in Petir Village, Rongkop, Gunungkidul. Morphological variation of Sandalwood in this area was relatively high. This research aimed to describe and classify sandalwood variations that exist in the research site based on the morphological characters. The materials used in this study were (1) sandalwood as the target species (including generative and vegetative organs) with purposive sampling and (2) Ximenia americana as the outgroup species. Data were analyzed using description and phylogeny analysis using maximum parsimony method with morphological markers. Morphological characters of Sandalwoods were varied in size and leave shapes, flowers perigonium, lobes shapes, appendices shapes, stem color, shapes and fruit size. There were two characters that have not been described in the previous sources in Indonesia, form of leaves (oblong and oval) and orange color of perigonium. Phylogeny results showed that a small leaf sandalwood character was closer to the outgroup character. Diagnostic characters explicitly classified small leaf size and leaf size large. Size of small leaf was from 2.8 -7.7 cm to 1.1-2.2 cm and from 7.9-10 cm to 3.1-4 cm for large leaf. Leaf form and colour of flower variation was found in each group of leaf size.


2008 ◽  
Vol 17 (2) ◽  
pp. 146 ◽  
Author(s):  
T. HIETARANTA ◽  
P. PARIKKA

The strawberry (Fragaria × ananassa) cultivars ‘Suvetar’ and ‘Valotar’ have been released from the breeding programme of MTT Agrifood Research Finland. Both new cultivars overwintered as well as the control cultivars ‘Jonsok’ and ‘Polka’. ‘Suvetar’ overwintered even better than ‘Polka’. ‘Suvetar’ was produced from the cross ‘Polka’ × ‘Emily’. It scored better than the control cultivars for sensory assessed skin resistance. Measured fruit firmness of ‘Suvetar’ was not significantly different from that of ‘Polka’, but was better than ‘Jonsok’. ‘Valotar’ originates from a cross between ‘Jewel’ and ‘Senga Sengana’. Its measured fruit firmness was better than that of ‘Jonsok’ and ‘Polka’. Considering sensory assessments, the good firmness seems to be largely due to good skin resistance. The fresh flavour, yield, fruit size and fruit appearance of ‘Suvetar’ and ‘Valotar’, together with improved fruit firmness, make both the new varieties suitable for the fresh fruit market.;


2016 ◽  
Vol 20 (2) ◽  
pp. 77
Author(s):  
NFN Karsinah ◽  
NFN Rebin ◽  
Lukitariati Sadwiyanti

<p>Evaluation of Mango Hybrids Derived from Crossing of Arumanis-143 with Three Red Mango Cultivars Based on Fruit Characters. Karsinah, Rebin, and Lukitariati Sadwiyanti. In order to improve the character of mango cv. Arumanis-143 that has green-skinned fruit to become red one, Indonesian Tropical Fruits Research Institute Solok had mainactivity, i.e. crossing between Arumanis-143 with Cukurgondang red clones. The objectives of this research were to evaluate the hybrids of mango cv. Arumanis-143 crossing with three red mango cultivars based on fruit characters, and to get 1&amp;ndash;2 new superior varieties candidate that had taste like Arumanis-143 with red/attractive fruit skin color. The research was conducted at Cukurgondang Experimental Field, Pasuruan, East Java from June to December 2011. Plant materials used were four cultivars of parents i.e. Arumanis- 143, Irwin, Gedong Gincu, and Saigon, and 27 hybrids from crossing between Arumanis-143 with Irwin, Arumanis-143 with Gedong Gincu, and Arumanis-143 with Saigon. The results indicated that there were 10 hybrids of 27 hybrids having bear fruits i.e. four hybrids from crossing between Arumanis-143 with Irwin, four hybrids from crossing between Arumanis-143 with Gedong Gincu, and two hybrids from crossing between Arumanis-143 with Saigon. The fruit shape of four hybrids from crossing between Arumanis-143 with Irwin were elliptic, fruits size varies with the smallest fruit weigh was F1-09 i.e. 202 g and the largest was F1-14 i.e. 443 g, fruits taste were sourish sweet-sweet, and the fruits skin color were green-yellow. Fruit shape of four hybrids from crossing between Arumanis-143 with Gedong Gincu were elliptic, fruit size varies with the smallest fruit weigh was F1-87 i.e. 281 g and the largest was F1-18 i.e. 519 g, fruits taste were vapidness sweet-very sweet, the fruits skin color were green-orangish green. The fruit shape of two hybrids from crossing between Arumanis-143 with Saigon were elliptic, fruit weigh of F1-45 was 141 g, fruit taste was sweet with yellowish-red fruit skin color and F1-49 was 232 g, fruit taste was sweet with yellowish green fruit skin color. Result of hibrids evaluation indicated that there was a new superior variety candidate that has taste like Arumanis-143 with yellowish-red fruit skin color.</p><p> </p><p><strong>Abstrak</strong></p><p>Dalam rangka memperbaiki karakter buah mangga Arumanis- 143 yang kulit buahnya berwarna hijau agar menjadi merah, Balai Penelitian Tanaman Buah Tropika, Solok, telah menyilangkan antara mangga Arumanis-143 dengan klon merah Cukurgondang. Penelitian bertujuan untuk mengetahui karakter hibrid hasil persilangan mangga Arumanis-143 dengan tiga kultivar mangga merah berdasarkan karakter buah dan untuk mendapatkan 1&amp;ndash;2 kandidat varietas unggul baru yang mempunyai cita rasa seperti Arumanis-143 dengan kulit buah berwarna merah/menarik. Penelitian dilaksanakan di Kebun Percobaan Cukurgondang, Pasuruan, Jawa Timur, dari bulan Juni sampai Desember 2011. Bahan tanaman yang digunakan ialah empat kultivar tanaman tetua, yaitu Arumanis-143, Irwin, Gedong Gincu, dan Saigon, serta 27 hibrid hasil persilangan Arumanis-143 dengan Irwin, Gedong Gincu, dan Saigon. Hasil penelitian menunjukkan bahwa dari 27 hibrid yang dievaluasi terdapat 10 hibrid yang berbuah, yaitu empat hibrid hasil persilangan antara Arumanis-143 dengan Irwin, empat hibrid hasil persilangan Arumanis-143 dengan Gedong Gincu, dan dua hasil persilangan Arumanis-143 dengan Saigon. Buah dari empat hibrid hasil persilangan antara mangga Arumanis-143 dengan Irwin berbentuk jorong, ukuran buah bervariasi, yang terkecil F1-09 berbobot 202 g dan terbesar F1-14 berbobot 443 g, rasa buah manis asam-manis, kulit buah berwarna hijau-kuning. Buah dari empat hibrid hasil persilangan Arumanis-143 dengan Gedong Gincu berbentuk jorong, ukuran buah bervariasi, yang terkecil F1-87 berbobot 281 g dan terbesar adalah F1-18 berbobot 519 g, rasa buah manis hambar-sangat manis, kulit buah berwarna hijau-hijau orange. Buah dari dua hibrid hasil persilangan Arumanis-143 dengan Saigon berbentuk jorong, buah F1-45 berbobot 141 g, rasa manis dengan kulit buah berwarna merah kekuningan dan buah F1-49 berbobot 232 g, rasa manis dengan kulit buah berwarna hijau kekuningan. Dari hasil evaluasi terhadap hibrid mangga diperoleh satu kandidat varietas unggul baru yang bercita rasa seperti Arumanis-143 dengan kulit buah berwarna merah kekuningan, yaitu F1-45.</p>


2019 ◽  
Vol 15 (2) ◽  
pp. 101-105
Author(s):  
Makful Makful ◽  
Kuswandi Kuswandi ◽  
Sahlan Sahlan ◽  
Mega Andini

Hybrid breeding of watermelon has led to the formation of high-yielding varieties. The objectives of the research were to determine the performance of hybrid watermelon of the Indonesian Tropical Fruit Research Institute (ITFRI) collection and to evaluate the superiority of each hybrid. The research has been conducted at Sumani Experimental Garden, ITFRI, Solok, West Sumatera, from April to July 2016. The material used consisted of 19 cross combinations. The data from this descriptive study were presented in the form of averages and pictures. The results showed that the hybrids that were tested showed diversity in size, fruit skin color, flesh color, skin thickness and fruit sweetness. Hybrids with three excellent characters, i.e. medium fruit weight, high total soluble solids (TSS) value, and moderate rind thickness, were not yet obtained. Hybrids that had the superiority in term of sweet taste and shelf life were BT1 × SGP, BT4 × BT4P, BT5 × BT4, BT6 × SGP and SGP × BT6. Keywords: hybrid, performance, total soluble solids, watermelon   ABSTRAK Pemuliaan hibrida pada semangka mengarah kepada pembentukan varietas berdaya hasil tinggi. Penelitian bertujuan untuk mengetahui keragaan hibrida semangka koleksi Balitbu Tropika dan untuk mengevaluasi keunggulan masing-masing hibrida Penelitian dilaksanakan di Kebun Percobaan Sumani, Balitbu Tropika, Solok, Sumatera Barat, dari bulan April sampai dengan Juli 2016. Materi yang digunakan terdiri dari 19 kombinasi persilangan. Data hasil penelitian deskriptif ini ditampilkan dalam bentuk rataan dan gambar. Hasil penelitian menunjukkan bahwa hibrida yang diuji memiliki keragaman dalam ukuran, warna kulit buah, warna daging buah, ketebalan kulit buah dan kemanisan. Belum ditemukan hibrida yang memiliki tiga karakter unggul, yang terdiri dari bobot buah sedang, total padatan terlarut (total soluble solid) tinggi, dan kulit buah agak tebal. Hibrida yang memiliki keunggulan berupa rasa manis dan tahan simpan adalah BT1 × SGP, BT4 × BT4P, BT5 × BT4, BT6 × SGP dan SGP × BT6. Kata kunci: hibrida, keragaan, semangka, total padatan terlarut


2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Ian S. E. Bally ◽  
◽  
Aureliano Bombarely ◽  
Alan H. Chambers ◽  
Yuval Cohen ◽  
...  

Abstract Background Mango, Mangifera indica L., an important tropical fruit crop, is grown for its sweet and aromatic fruits. Past improvement of this species has predominantly relied on chance seedlings derived from over 1000 cultivars in the Indian sub-continent with a large variation for fruit size, yield, biotic and abiotic stress resistance, and fruit quality among other traits. Historically, mango has been an orphan crop with very limited molecular information. Only recently have molecular and genomics-based analyses enabled the creation of linkage maps, transcriptomes, and diversity analysis of large collections. Additionally, the combined analysis of genomic and phenotypic information is poised to improve mango breeding efficiency. Results This study sequenced, de novo assembled, analyzed, and annotated the genome of the monoembryonic mango cultivar ‘Tommy Atkins’. The draft genome sequence was generated using NRGene de-novo Magic on high molecular weight DNA of ‘Tommy Atkins’, supplemented by 10X Genomics long read sequencing to improve the initial assembly. A hybrid population between ‘Tommy Atkins’ x ‘Kensington Pride’ was used to generate phased haplotype chromosomes and a highly resolved phased SNP map. The final ‘Tommy Atkins’ genome assembly was a consensus sequence that included 20 pseudomolecules representing the 20 chromosomes of mango and included ~ 86% of the ~ 439 Mb haploid mango genome. Skim sequencing identified ~ 3.3 M SNPs using the ‘Tommy Atkins’ x ‘Kensington Pride’ mapping population. Repeat masking identified 26,616 genes with a median length of 3348 bp. A whole genome duplication analysis revealed an ancestral 65 MYA polyploidization event shared with Anacardium occidentale. Two regions, one on LG4 and one on LG7 containing 28 candidate genes, were associated with the commercially important fruit size characteristic in the mapping population. Conclusions The availability of the complete ‘Tommy Atkins’ mango genome will aid global initiatives to study mango genetics.


2000 ◽  
Vol 71 (1-2) ◽  
pp. 89-92 ◽  
Author(s):  
Anju Puri ◽  
R Sahai ◽  
Kiran L Singh ◽  
R.P Saxena ◽  
J.S Tandon ◽  
...  

Phytotaxa ◽  
2017 ◽  
Vol 318 (1) ◽  
pp. 1 ◽  
Author(s):  
ELTON M. C. LEME ◽  
SASCHA HELLER ◽  
GEORG ZIZKA ◽  
HEIDEMARIE HALBRITTER

The authors provide a new circumscription for genera of the “Cryptanthoid complex” of Bromeliaceae subfam. Bromelioideae, originally composed of Cryptanthus, Lapanthus, Orthophytum, and Sincoraea, on the basis of new or re-evaluated ecological, geographical and morphological evidence, as well as molecular phylogenies. A new generic status is proposed for Cryptanthus subg. Hoplocryptanthus and two new genera, Forzzaea, and Rokautskyia, as well as four new subgenera in Orthophytum (Capixabanthus, Clavanthus, Krenakanthus, and Orthocryptanthus) are described to render taxonomic units monophyletic. The recognized taxa are well circumscribed by the combination of geographical range, ecology and morphological characters (sex distribution, leaf succulence, sepal and petal size and connation, petal appendages, pollen and stigma morphology, fruit size, calyx persistency, seed size and number per fruit). Field collected living specimens of 78 of the 81 species of Cryptanthus s.l., all species of Lapanthus, 58 of the 59 species of Orthophytum, and all species of Sincoraea were analysed in habitat and/or in cultivation, allowing the documentation and illustration of new and underutilized characters. The molecular analysis incorporated 91 accessions representing 33 species of Cryptanthus, all species (3) of Lapanthus, 42 species of Orthophytum, and 9 species of Sincoraea, including the type species for the first three genera and four outgroup taxa. The results suggest, that some morphological characters generally considered homoplasious for Bromelioideae, for the “Cryptanthoid complex”, are not homoplasious at least within the obtained, biogeographycally well delimited clades and their taxonomical utility is redeemed.


2009 ◽  
Vol 34 (1) ◽  
pp. 220-242 ◽  
Author(s):  
Anthony R. Magee ◽  
Ben-Erik van Wyk ◽  
Patricia M. Tilney

A comprehensive taxonomic revision of the genus Notobubon (Apiaceae) is presented. Twelve woody evergreen species are recognised, all (with the exception of N. laevigatum) endemic to the Cape Floristic Region of South Africa. The taxonomy of these prominent, though poorly collected, species has until now been problematic. They are distinguished from one another by their habit (size and branching pattern), the overall shape, size, and colour of the ultimate leaflet segments, the inflorescence structure (peduncle length, number, and length of rays in the primary umbel), the fruit morphology (fruit size, presence or absence of wings), and the fruit anatomy (symmetry of the mericarps, presence or absence of additional rib vittae, size of commissural vittae). Species relationships are assessed in the form of a cladistic analysis of 26 morphological characters, resulting in a well-resolved phylogenetic hypothesis. A comprehensive key to the species, their correct nomenclature, and typification, together with descriptions and known geographical distribution for all the species are presented and illustrated.


2012 ◽  
Vol 14 (1) ◽  
pp. 19 ◽  
Author(s):  
Zulkarnain Zulkarnain ◽  
Lizawati Lizawati

The aim of this study was to develop an efficient method for the induction of embryogenic callus formation for in vitro propagation ofjatropha. Plant materials used were 30-days old in vitro seedlings, cut into hypocotyl and cotyledon (lower, middle and upper) sections.Medium used was MS composition supplemented with vitamins, 3% sucrose, 0.7% agar at pH 5.8 ± 1, and 2,4-D (0, 1, 2, 3, 4 dan5 mg l-1). Cultures were kept at temperature of 25 ± 1 0C with 50 μmol m-2 s-1 light intensity and 16-h photoperiod. The results indicated thatthe rate of callus formation depended on the source of explant, the application of 2,4-D, and the interaction of both. The fastest callusproliferation (2.33 days following initiation) was obtained on cotyledon explants cultured on medium without 2,4-D. The explant sourcesand 2,4-D concentrations also showed significant effect on the percentage of explant forming callus. The most callus formation (88.33%)was obtained on middle cotyledon cultured on 3 mg l-1 2,4-D, whereas the fewest (6.84%) was found on upper cotyledon cultured on mediumwithout 2,4-D. The colour of callus was dominated by white, light yellow, cream and brown with mostly compact structure, particularly onhypocotyl cultured on medium without 2,4-D. The texture of callus formed on hypocotyl treated with up to 4 mg l -1 2,4-D was dominatedby coarse appearance. In contrast, majority of callus proliferated on hypocotyl treated with 5 mg l -1 2,4-D or cotyledon treated with orwithout 2,4-D produced callus with smooth texture %.


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