scholarly journals IMPROVEMENT OF EARLY MATURITY IN RICE VARIETY BY MARKER ASSISTED BACKCROSS BREEDING OF Hd2 GENE

2014 ◽  
Vol 15 (2) ◽  
pp. 55
Author(s):  
Fatimah Fatimah ◽  
Joko Prasetiyono ◽  
Ahmad Dadang ◽  
Tasliah Tasliah
Keyword(s):  
Agronomic Traits ◽  
Rice Variety ◽  
Heading Date ◽  
Rice Production ◽  
Early Maturity ◽  
Breeding Lines ◽  
Early Maturing ◽  
Two Generations ◽  
Donor Parent ◽  
Marker Assisted Backcross Breeding

Early-maturing and high-yielding rice variety is very useful for increasing rice production in Indonesia. The aim of this research was to develop new lines of Indonesian rice containing Hd2 gene using Code variety as a recipient parent and Nipponbare variety as a donor parent through targetted MAB approach using RM1362 and RM7601 in chromosom 7 for foreground selection. After two generations of backcrossing, the positive alleles of Hd2 gene from Nipponbare had successfully trans-ferred into Code. The plant number CdNp_29 in BC2F2 popula-tion had the highest genome recovery of 82.7%. The twelve BC2F3 plants were selected for self-pollination to generate BC2F4. These selected lines that carried the Hd2 gene were screened in the greenhouse for the evaluation of heading date and agronomic traits. All improved lines had Hd2 gene similar to the donor parent Nipponbare. The heading date of the breeding lines ranged from 73 to 89 days (Code 85 days) or fill the third criterion of rice maturity that is 103-104 days compared to Code of 116-119 days, whereas their agronomic performances were similar with that of Code. Application of MABc for im-proving rice early maturity has accelerated the development and selection in early generation of superior lines having genetic background of Code. It is expected that the newly developed lines of Code will be utilized to increase rice production in Indonesia.

scholarly journals IMPROVEMENT OF EARLY MATURITY IN RICE VARIETY BY MARKER ASSISTED BACKCROSS BREEDING OF Hd2 GENE

2014 ◽  
Vol 15 (2) ◽  
pp. 55
Author(s):  
Fatimah Fatimah ◽  
Joko Prasetiyono ◽  
Ahmad Dadang ◽  
Tasliah Tasliah
Keyword(s):  
Agronomic Traits ◽  
Rice Variety ◽  
Heading Date ◽  
Rice Production ◽  
Early Maturity ◽  
Breeding Lines ◽  
Early Maturing ◽  
Two Generations ◽  
Donor Parent ◽  
Marker Assisted Backcross Breeding

Early-maturing and high-yielding rice variety is very useful for increasing rice production in Indonesia. The aim of this research was to develop new lines of Indonesian rice containing Hd2 gene using Code variety as a recipient parent and Nipponbare variety as a donor parent through targetted MAB approach using RM1362 and RM7601 in chromosom 7 for foreground selection. After two generations of backcrossing, the positive alleles of Hd2 gene from Nipponbare had successfully trans-ferred into Code. The plant number CdNp_29 in BC2F2 popula-tion had the highest genome recovery of 82.7%. The twelve BC2F3 plants were selected for self-pollination to generate BC2F4. These selected lines that carried the Hd2 gene were screened in the greenhouse for the evaluation of heading date and agronomic traits. All improved lines had Hd2 gene similar to the donor parent Nipponbare. The heading date of the breeding lines ranged from 73 to 89 days (Code 85 days) or fill the third criterion of rice maturity that is 103-104 days compared to Code of 116-119 days, whereas their agronomic performances were similar with that of Code. Application of MABc for im-proving rice early maturity has accelerated the development and selection in early generation of superior lines having genetic background of Code. It is expected that the newly developed lines of Code will be utilized to increase rice production in Indonesia.

scholarly journals Field Evaluation Of Conde Progenies With Early Heading Date And Resistance To Bacterial Leaf Blight

2020 ◽  
Vol 23 (2) ◽  
pp. 87
Author(s):  
Fatimah Fatimah ◽  
Joko Prasetiyono ◽  
Ahmad Dadang ◽  
Tasliah Tasliah ◽  
Masumah Masumah
Keyword(s):  
Agronomic Traits ◽  
Rice Variety ◽  
Heading Date ◽  
Field Evaluation ◽  
Leaf Blight ◽  
Bacterial Leaf Blight ◽  
Early Maturity ◽  
South Sulawesi ◽  
Donor Parent ◽  
Marker Assisted Backcrossing

Heading date is one of key traits in rice adaptation to different environments. Rice with early maturity is very useful for increasing rice production extensively especially in Indonesia. The improvement of rice variety Conde for heading date and resistance to bacterial leaf blight (BLB) was developed through Marker Assisted Backcrossing approach (MAB). Xa7 derived from Conde as recipient parents for resistance to BLB and Hd2 gene derived from Nipponbare as a donor parent for heading date. The aim of this research was to evaluate the performance of heading date and agronomic traits of 85 BC2F4 lines in two field stations, Sukamandi (West Java) and Maros (South Sulawesi). Molecular analysis of Xa7 on chromosome 6 and Hd2 gene on chromosome 7 and agronomic performance was evaluated. Foreground analysis resulted 41% for Xa7 and 66.7% for Hd2 gene in homozygous condition. The majority of lines have similar heading date and yield with Conde and more resistance to bacterial leaf blight (BLB) than Conde. Six lines flowered earlier 5 days than Conde in Sukamandi and three lines flowered earlier 3 days in Maros. Twelve lines with flowered earlier than Conde, resistance to BLB and similar yield with Conde were selected for background analysis resulted 93% genome recovery. These lines would be further evaluated in preliminary yield trail for their stability.

scholarly journals Pembentukan Genotipe Padi Berumur Sangat Genjah melalui Kultur Antera

2016 ◽  
Vol 18 (2) ◽  
pp. 54
Author(s):  
Iswari S. Dewi ◽  
A. Dinar Ambarwati ◽  
Aniversari Apriana ◽  
Atmitri Sisharmini ◽  
Ida H. Somantri ◽  
...  
Keyword(s):  
Anther Culture ◽  
Callus Induction ◽  
Culture Media ◽  
Oryza Sativa L ◽  
Doubled Haploids ◽  
Heading Date ◽  
Population Increase ◽  
Early Maturity ◽  
Early Maturing ◽  
Pure Lines

<p>Development of Very Early Maturing Rice Genotypes through Anther Culture. Iswari S. Dewi, A. Dinar Ambarwati, Aniversari Apriana, Atmitri Sisharmini, Ida H. Somantri, Bambang Suprihatno, and Iman Ridwan. Rice is the most important food crop in Indonesia. Increase in production is needed due to population increase. Rice production in rainfed area is contributed the second after irrigated area. Rainfed condition requiring very early maturity (90-104 days) varieties. Rice anther culture can be applied to accelerate obtainment of doubled haploids (DHs) or pure lines needed in rice breeding. The experiment was aimed to obtain pure lines for developing very early maturing and high yielding rice varieties. Materials used for anther culture were F1s of Fatmawati/Kinamase, Inpari 1/Kinamase, Fatmawati/ Waseaikoku, Inpari 1/Waseaikoku, Fatmawati/IR71146, Inpari 1/IR71146, OM4495/Silugonggo, IR7146/Dodokan, and IR71730/OM1490. Anther culture media were N6 + NAA 2,0 mg/l + kinetin 0,5 mg/l for callus induction, MS+ NAA 0,5 mg/l + kinetin 2,0 mg/l for plantlet regeneration, and MS + 0,5 mg/l IBA for rooting. Putrescine 10-3 M was added to callus induction and regeneration media. The results shown that calli forming green plantlet (CFGP) were ranged from 0.25 to 83.33%. Fatmawati/Kinamase gave the highest CFGP (245 calli), followed by Inpari 1/Kinamase (78 calli) and Fatmawati/ Waseaikoku (68 calli). Total green plantlets obtained were 2.038 plantlets. After plantlet acclimatization and greenhouse grow-out, we obtained 507 DHs. The evaluation of 100 DHs at farmer field (Ciranjang District in Cianjur), based on their 50% heading date of 65 days, resulted in 33 lines cathegorized as very early maturing lines (+100 days). They were 18 lines from Fatmawati/Kinamase, 5 lines from Inpari 1/Kinamase, 8 lines from Fatmawati/Waseaikoku, and 2 lines from Inpari 1/ Waseaikoku.</p><p> </p><p><strong>Abstrak</strong></p><p>Padi (Oryza sativa L.) merupakan komoditi pangan terpenting di Indonesia. Peningkatan produksi diperlukan seiring dengan peningkatan jumlah penduduk. Lahan sawah tadah hujan merupakan lumbung padi kedua setelah sawah irigasi. Kondisi lahan sawah tadah hujan memerlukan varietas-varietas padi berumur sangat genjah (90-104 hari). Teknik kultur antera dapat digunakan untuk mempercepat perolehan tanaman dihaploid (DH) atau galur murni dalam pemuliaan padi. Penelitian ini bertujuan untuk mendapatkan galur-galur murni yang akan digunakan dalam perakitan padi berdaya hasil tinggi dan berumur sangat genjah. Bahan tanaman yang digunakan untuk kultur antera adalah malai dari tanaman F1 hasil persilangan Fatmawati/Kinamase, Inpari 1/Kinamase, Fatmawati/Waseaikoku, Inpari 1/Waseaikoku, Fatmawati/IR71146, Inpari 1/ IR71146, OM4495/Silugonggo, IR7146/Dodokan, dan IR71730/OM1490. Media kultur antera adalah N6 + NAA 2,0 mg/l + kinetin 0,5 mg/l untuk media induksi kalus, MS+ NAA 0,5 mg/l + kinetin 2,0 mg/l untuk media regenerasi, dan MS + 0,5 mg/l IBA untuk media perakaran. Putresine 10-3 M ditambahkan pada media induksi kalus dan regenerasi. Hasil penelitian menunjukkan bahwa kalus yang menghasilkan tanaman hijau (KMTH) berkisar antara 0,25-83,33%. Persilangan Fatmawati/ Kinamase memberikan KMTH tertinggi (245 kalus), diikuti oleh Inpari 1/Kinamase (78 kalus) dan Fatmawati/ Waseaikoku (68 kalus). Total tanaman hijau yang diperoleh adalah 2.038 planlet dihaploid, namun diperoleh 507 tanaman setelah planlet diaklimatisasi dan tanaman ditumbuhkan di rumah kaca. Evaluasi terhadap 100 DH dilakukan di lahan petani Ciranjang, Cianjur. Berdasarkan hari berbunga 50% (65 hari setelah semai), diperoleh 33 galur yang termasuk kategori sangat genjah (dipanen +100 hari). Galur-galur tersebut adalah 18 galur dari persilangan Fatmawati/Kinamase, 5 galur dari persilangan Inpari 1/Kinamase, 8 galur dari persilangan Fatmawati/ Waseaikoku, dan 2 galur dari persilangan Inpari 1/ Waseaikoku.</p>

scholarly journals Identification of fgr gene and preliminary evaluation for agronomic traits of pandan sticky rice variety (Oryza sativa L.)

2019 ◽  
Vol 02 ◽  
pp. 39-48
Author(s):  
Toan D. Pham
Keyword(s):  
Oryza Sativa ◽  
Agronomic Traits ◽  
Oryza Sativa L ◽  
Rice Variety ◽  
Rice Production ◽  
Molecular Techniques ◽  
High Yield ◽  
Preliminary Evaluation ◽  
Rice Varieties ◽  
Fgr Gene

Rice (Oryza sativa L.) is the most important food crop in Vietnam particularly in the Mekong Delta. Screening of good quality and high yield rice varieties are needed for rice production in Vietnam. The purpose of this study was to use molecular techniques to identify fgr gene and to evaluate preliminarily agronomic traits of pandan sticky rice variety. The results showed that this variety contained fgr gene. The fragrant allele was amplified by ESP - IFAP primers with a product of 255 bp in size. Similarly, evaluation of agronomic traits showed that the pandan sticky rice variety displayed many desirable characteristics such as plant height of 108 cm, panicle length of 25.6 cm, seed/panicle 135, 100-grain weight 2.07 g, pandan smell, level 2 of alkali digestion, gel consistency 93 mm. These results were useful information and could be applied for improving and providing pandan sticky rice variety for rice production.

scholarly journals High-Quality Genomes and High-Density Genetic Map Facilitate the Identification of Genes From a Weedy Rice

2021 ◽  
Vol 12 ◽  
Author(s):  
Fei Li ◽  
Zhenyun Han ◽  
Weihua Qiao ◽  
Junrui Wang ◽  
Yue Song ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Genetic Map ◽  
Agronomic Traits ◽  
Rice Variety ◽  
Heading Date ◽  
High Density ◽  
Weedy Rice ◽  
Rice Breeding ◽  
Cultivated Rice ◽  
High Quality

Genes have been lost or weakened from cultivated rice during rice domestication and breeding. Weedy rice (Oryza sativa f. spontanea) is usually recognized as the progeny between cultivated rice and wild rice and is also known to harbor an gene pool for rice breeding. Therefore, identifying genes from weedy rice germplasms is an important way to break the bottleneck of rice breeding. To discover genes from weedy rice germplasms, we constructed a genetic map based on w-hole-genome sequencing of a F2 population derived from the cross between LM8 and a cultivated rice variety. We further identified 31 QTLs associated with 12 important agronomic traits and revealed that ORUFILM03g000095 gene may play an important role in grain length regulation and participate in grain formation. To clarify the genomic characteristics from weedy rice germplasms of LM8, we generated a high-quality genome assembly using single-molecule sequencing, Bionano optical mapping, and Hi-C technologies. The genome harbored a total size of 375.8 Mb, a scaffold N50 of 24.1 Mb, and originated approximately 0.32 million years ago (Mya) and was more closely related to Oryza sativa ssp. japonica. and contained 672 unique genes. It is related to the formation of grain shape, heading date and tillering. This study generated a high-quality reference genome of weedy rice and high-density genetic map that would benefit the analysis of genome evolution for related species and suggested an effective way to identify genes related to important agronomic traits for further rice breeding.

scholarly journals Identification of Heading Date Six (Hd6) Gene Derived from Rice Mutant Varieties

2017 ◽  
Vol 9 (1) ◽  
pp. 105
Author(s):  
Aryanti Aryanti ◽  
Ita Dwimahyani ◽  
Ishak Ishak
Keyword(s):  
Heading Date ◽  
Positive Control ◽  
Parent Material ◽  
Mutation Induction ◽  
Early Maturity ◽  
Breeding Programs ◽  
Cross Breeding ◽  
Rice Mutant ◽  
Early Maturing ◽  
Young Leaves

<p>Genes which were associated with flowering time to indicate the early maturity is known as heading date (<em>Hd</em>). Heading date six (<em>Hd6)</em> gene<em> </em>was identified from rice mutant varieties were Atomita 2, Atomita 3, Atomita 4, Bestari, Cilosari, Diah Suci, Sidenuk, Kahayan, Mayang, Meraoke, Mira-1, Pandan Putri, Superwin, Suluttan Unsrat 1, Suluttan Unsrat 2, Winongo, Woyla, Yuwono, while the rice var. Nipponbare was used as a positive control. All of rice mutant varieties derived from mutation induction by the dose of 0.2 kGy. The aim of this experiment was to find out the data base of mutant varieties which could be used as parent material with earlier maturity trait genetically. To obtain the DNA of plants, young leaves of each variety were extracted by liquid nitrogen, and then lysis and extracted by Kit Plant Genomic DNA. The amplification of DNA with 7 primers of <em>Hd6</em> conducted of 40 cycles by PCR and were continues to separated by 1 % agarose. The results were shown that the rice Mira-1 and Bestari varieties obtained from mutation of Cisantana highly different from one to another on 7 primers of <em>Hd6</em> used. Mayang variety from mutation of cross breeding between Cilosari and IR64, Pandan putri from Pandan wangi and Woyla from mutation of cross breeding from Atomita 2 and IR64 were highly different with those of their parents. Identification of <em>Hd6</em> gene on Sidenuk variety was shown the same bands pattern with Nipponbare as control positive toward all primers used, this variety would be better for earlier maturity parent material compared to others. The information could be useful for breeding programs aiming to develop early maturing widely adaptive and high yielding rice cultivars.</p>

scholarly journals Meta-QTL and ortho-MQTL analyses identified genomic regions controlling rice yield, yield-related traits and root architecture under water deficit conditions

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Bahman Khahani ◽  
Elahe Tavakol ◽  
Vahid Shariati ◽  
Laura Rossini
Keyword(s):  
Water Deficit ◽  
Root Architecture ◽  
Agronomic Traits ◽  
Heading Date ◽  
Dry Weight ◽  
Genetic Dissection ◽  
Rice Varieties ◽  
Average Confidence ◽  
Stable Qtls ◽  
Genomic Regions

AbstractMeta-QTL (MQTL) analysis is a robust approach for genetic dissection of complex quantitative traits. Rice varieties adapted to non-flooded cultivation are highly desirable in breeding programs due to the water deficit global problem. In order to identify stable QTLs for major agronomic traits under water deficit conditions, we performed a comprehensive MQTL analysis on 563 QTLs from 67 rice populations published from 2001 to 2019. Yield and yield-related traits including grain weight, heading date, plant height, tiller number as well as root architecture-related traits including root dry weight, root length, root number, root thickness, the ratio of deep rooting and plant water content under water deficit condition were investigated. A total of 61 stable MQTLs over different genetic backgrounds and environments were identified. The average confidence interval of MQTLs was considerably refined compared to the initial QTLs, resulted in the identification of some well-known functionally characterized genes and several putative novel CGs for investigated traits. Ortho-MQTL mining based on genomic collinearity between rice and maize allowed identification of five ortho-MQTLs between these two cereals. The results can help breeders to improve yield under water deficit conditions.

Response of Early, Medium, and Late Maturing Peanut Breeding Lines to Field Epidemics of Tomato Spotted Wilt

1999 ◽  
Vol 26 (2) ◽  
pp. 100-106 ◽  
Author(s):  
A. K. Culbreath ◽  
J. W. Todd ◽  
D. W. Gorbet ◽  
S. L. Brown ◽  
J. A. Baldwin ◽  
...  
Keyword(s):  
High Oleic Acid ◽  
Breeding Programs ◽  
Breeding Lines ◽  
High Oleic ◽  
Tomato Spotted Wilt ◽  
Arachis Hypogaea L ◽  
Early Maturing ◽  
Late Maturity ◽  
Field Plots ◽  
Maturity Groups

Abstract Epidemics of tomato spotted wilt, caused by tomato spotted wilt Tospovirus (TSWV), were monitored in field plots of runner-type peanut (Arachis hypogaea L.) cultivars Georgia Green and Georgia Runner and numerous breeding lines from four different breeding programs as part of efforts toward characterizing breeding lines with potential for release as cultivars. Breeding lines were divided into early, medium and late maturity groups. The tests were conducted near Attapulgus, GA and Marianna, FL in 1997 and in Tifton, GA and Marianna, FL in 1998. Epidemics in some early and medium maturing breeding lines, including some genotypes with high oleic acid oil chemistry, were comparable to those in Georgia Green, the cultivar most frequently used in the southeastern U.S. for suppression of spotted wilt epidemics. No early maturing breeding lines had lower spotted wilt final intensity ratings or higher yields than Georgia Green. However, spotted wilt intensity ratings in some late maturing lines and a smaller number of medium maturing lines were significantly lower than those of Georgia Green. Several of those lines also produced greater pod yields than Georgia Green. Results from these experiments indicated that there is potential for improving management of spotted wilt though development of cultivars that suppress spotted wilt epidemics more than currently available cultivars. There was no indication that differences in spotted wilt ratings corresponded to differences in numbers of thrips adults or larvae.

scholarly journals Improvement of Upland Rice Variety by Pyramiding Drought Tolerance QTL with Two Major Blast Resistance Genes for Sustainable Rice Production

Rice Science ◽  
2021 ◽  
Vol 28 (5) ◽  
pp. 493-500
Author(s):  
Vishalakshi Balija ◽  
Umakanth Bangale ◽  
Senguttuvel Ponnuvel ◽  
Kalyani Makarand Barbadikar ◽  
Srinivas Prasad Madamshetty ◽  
...  
Keyword(s):  
Drought Tolerance ◽  
Resistance Genes ◽  
Upland Rice ◽  
Blast Resistance ◽  
Rice Variety ◽  
Rice Production ◽  
Blast Resistance Genes

scholarly journals Yield QTL analysis of Oryza sativa x O. glumaepatula introgression lines

2013 ◽  
Vol 48 (3) ◽  
pp. 280-286 ◽  
Author(s):  
Priscila Nascimento Rangel ◽  
Rosana Pereira Vianello ◽  
Arthur Tavares Oliveira Melo ◽  
Paulo Hideo Nakano Rangel ◽  
João Antônio Mendonça ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Interspecific Cross ◽  
Rice Genome ◽  
Introgression Lines ◽  
Wild Accession ◽  
Cultivated Rice ◽  
Two Generations ◽  
Ssr Loci ◽  
Donor Parent ◽  
Effect On Yield

The objective of this work was to evaluate the yield performance of two generations (BC2F2 and BC2F9) of introgression lines developed from the interspecific cross between Oryza sativa and O. glumaepatula, and to identify the SSR markers associated to yield. The wild accession RS‑16 (O. glumaepatula) was used as donor parent in the backcross with the high yielding cultivar Cica‑8 (O. sativa). A set of 114 BC2F1 introgression lines was genotyped with 141 polymorphic SSR loci distributed across the whole rice genome. Molecular analysis showed that in average 22% of the O. glumaepatula genome was introgressed into BC2F1 generation. Nine BC2F9 introgression lines had a significantly higher yield than the genitor Cica‑8, thus showing a positive genome interaction among cultivated rice and the wild O. glumaepatula. Seven QTL were identified in the overall BC2F2, with one marker interval (4879‑EST20) of great effect on yield. The alleles with positive effect on yield came from the cultivated parent Cica‑8.

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