Identification of fgr gene and preliminary evaluation for agronomic traits of pandan sticky rice variety (Oryza sativa L.)

Rice (Oryza sativa L.) is the most important food crop in Vietnam particularly in the Mekong Delta. Screening of good quality and high yield rice varieties are needed for rice production in Vietnam. The purpose of this study was to use molecular techniques to identify fgr gene and to evaluate preliminarily agronomic traits of pandan sticky rice variety. The results showed that this variety contained fgr gene. The fragrant allele was amplified by ESP - IFAP primers with a product of 255 bp in size. Similarly, evaluation of agronomic traits showed that the pandan sticky rice variety displayed many desirable characteristics such as plant height of 108 cm, panicle length of 25.6 cm, seed/panicle 135, 100-grain weight 2.07 g, pandan smell, level 2 of alkali digestion, gel consistency 93 mm. These results were useful information and could be applied for improving and providing pandan sticky rice variety for rice production.

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The Development and Characterization of Near-Isogenic and Pyramided Lines Carrying Resistance Genes to Brown Planthopper with the Genetic Background of Japonica Rice (Oryza sativa L.)

Plants ◽

10.3390/plants8110498 ◽

2019 ◽

Vol 8 (11) ◽

pp. 498

Author(s):

Cuong D. Nguyen ◽

Holden Verdeprado ◽

Demeter Zita ◽

Sachiyo Sanada-Morimura ◽

Masaya Matsumura ◽

...

Keyword(s):

Oryza Sativa ◽

Resistance Genes ◽

Genetic Background ◽

Oryza Sativa L ◽

Rice Variety ◽

Brown Planthopper ◽

Nilaparvata Lugens ◽

Japonica Rice ◽

Rice Varieties ◽

Nilaparvata Lugens Stål

The brown planthopper (BPH: Nilaparvata lugens Stål.) is a major pest of rice, Oryza sativa, in Asia. Host plant resistance has tremendous potential to reduce the damage caused to rice by the planthopper. However, the effectiveness of resistance genes varies spatially and temporally according to BPH virulence. Understanding patterns in BPH virulence against resistance genes is necessary to efficiently and sustainably deploy resistant rice varieties. To survey BPH virulence patterns, seven near-isogenic lines (NILs), each with a single BPH resistance gene (BPH2-NIL, BPH3-NIL, BPH17-NIL, BPH20-NIL, BPH21-NIL, BPH32-NIL and BPH17-ptb-NIL) and fifteen pyramided lines (PYLs) carrying multiple resistance genes were developed with the genetic background of the japonica rice variety, Taichung 65 (T65), and assessed for resistance levels against two BPH populations (Hadano-66 and Koshi-2013 collected in Japan in 1966 and 2013, respectively). Many of the NILs and PYLs were resistant against the Hadano-66 population but were less effective against the Koshi-2013 population. Among PYLs, BPH20+BPH32-PYL and BPH2+BPH3+BPH17-PYL granted relatively high BPH resistance against Koshi-2013. The NILs and PYLs developed in this research will be useful to monitor BPH virulence prior to deploying resistant rice varieties and improve rice’s resistance to BPH in the context of regionally increasing levels of virulence.

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Utilisation of anther culture in rice (Oryza sativa L.) breeding in Italy

Australian Journal of Experimental Agriculture ◽

10.1071/ea9940911 ◽

1994 ◽

Vol 34 (7) ◽

pp. 911 ◽

Cited By ~ 1

Author(s):

AM Callegarin ◽

K Perfanov ◽

G Dorotea ◽

G Baldi

Keyword(s):

Oryza Sativa ◽

Anther Culture ◽

Grain Quality ◽

Agronomic Traits ◽

Oryza Sativa L ◽

Market Demand ◽

Breeding Methods ◽

Rice Varieties ◽

Hybrid Lines

Increasing market demand has led to efforts to select for non-sticky, long-grain rice varieties suited to the Italian environment. The variability of recombinant populations derived from crosses between Italian and USA genotypes was exploited. Together with the conventional breeding methods, in vitro anther culture was used to obtain homozygous lines from crosses more quickly. Moreover, hybrid lines were obtained from crosses otherwise impossible to exploit owing to sterility in the F, generation (namely those resulting from crosses involving semi-dwarf USA varieties). Plant regeneration was obtained from 6 F, hybrids whose parents were chosen for their grain quality or their suitability to the Italian environment. About 5500 plantlets were regenerated. Of these, 1000 were albino, and about 50% of the green ones died after transplanting into soil; 65% of the surviving plants were sterile, so about 1000 progeny were obtained (30-445 for each cross). Progeny with desirable characteristics were evaluated for agronomic traits. On the basis of these results, limits and possibilities of anther-culture breeding in rice are discussed.

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Molecular Breeding for Improving Productivity of Oryza sativa L. cv. Pusa 44 under Reproductive Stage Drought Stress through Introgression of a Major QTL, qDTY12.1

Genes ◽

10.3390/genes12070967 ◽

2021 ◽

Vol 12 (7) ◽

pp. 967

Author(s):

Kyaw Swar Oo ◽

Subbaiyan Gopala Krishnan ◽

Kunnummal Kurungara Vinod ◽

Gaurav Dhawan ◽

Priyanka Dwivedi ◽

...

Keyword(s):

Drought Stress ◽

Oryza Sativa L ◽

Rice Variety ◽

Phenotypic Selection ◽

Rice Production ◽

Reproductive Stage ◽

High Yield ◽

Recurrent Parent ◽

Global Food Security ◽

Successful Recovery

Increasing rice production is quintessential to the task of sustaining global food security, as a majority of the global population is dependent on rice as its staple dietary cereal. Among the various constraints affecting rice production, reproductive stage drought stress (RSDS) is a major challenge, due to its direct impact on grain yield. Several quantitative trait loci (QTLs) conferring RSDS tolerance have been identified in rice, and qDTY12.1 is one of the major QTLs reported. We report the successful introgression of qDTY12.1 into Pusa 44, a drought sensitive mega rice variety of the northwestern Indian plains. Marker-assisted backcross breeding (MABB) was adopted to transfer qDTY12.1 into Pusa 44 in three backcrosses followed by four generations of pedigree selection, leading to development of improved near isogenic lines (NILs). Having a recurrent parent genome (RPG) recovery ranging from 94.7–98.7%, the improved NILs performed 6.5 times better than Pusa 44 under RSDS, coupled with high yield under normal irrigated conditions. The MABB program has been modified so as to defer background selection until BC3F4 to accelerate generational advancements. Deploying phenotypic selection alone in the early backcross generations could help in the successful recovery of RPG. In addition, the grain quality could be recovered in the improved NILs, leading to superior selections. Owing to their improved adaptation to drought, the release of improved NILs for regions prone to intermittent drought can help enhance rice productivity and production.

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The role of effluent water irrigation in the mineral absorption of aerobic rice varieties (Oryza sativa L.)

Cereal Research Communications ◽

10.1007/s42976-020-00117-x ◽

2020 ◽

Author(s):

Marks Ibadzade ◽

Ágnes Kun ◽

Árpád Székely ◽

Tímea Szalóki ◽

Károly Penksza ◽

...

Keyword(s):

Oryza Sativa ◽

River Water ◽

Irrigation Water ◽

Oryza Sativa L ◽

Rice Production ◽

Water Bodies ◽

Aerobic Rice ◽

Rice Varieties ◽

Lysimeter Experiment ◽

The Impact

AbstractQuality and quantity of different irrigation water types from conventional and alternative sources have a significant role on the productive parameters and chemical composition of crop plants. Appropriate alternative water sources and the reutilization of agricultural effluents can reduce the impact of rice production and animal husbandry on the natural water bodies. In the present study, influence of four different types of irrigation water was analyzed on the nutrient uptake (P, K, Ca, Mg, Na) of aerobic rice (Oryza sativa L.) in a complex lysimeter experiment in two consecutive years. Early maturing Hungarian rice varieties (M 488 and Janka) were irrigated with traditional river water (RW) and different alternative irrigation sources to evaluate the feasibility of a sodium containing intensive fish farm effluent with (EWG) or without (EW) gypsum supplementation and with the addition of natural river water (EWGR). Significant effects on the mineral content of the aboveground biomass were measured. P uptake by M 488 and Janka decreased after the irrigation with EW in 2017. In case of EW, EWG and EWGR, the Na content increased significantly (p ≤ 0.05) in both varieties; however, pre-treatment of salt containing effluent waters can moderate the stress level. As a consequence, the ability of both rice varieties to absorb Na suggests that rice production could be conditionally part of bioremediation of salt-affected soils and water bodies.

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Karakteristik Agronomis Tiga Varietas Padi (Oryza sativa L.) pada Dua Sistem Tanam Benih di Lahan Pasang Surut

Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) ◽

10.24831/jai.v46i2.15781 ◽

2018 ◽

Vol 46 (2) ◽

pp. 140

Author(s):

Haris Kriswantoro ◽

Etty Safriyani ◽

Purwaningsih , ◽

Dan Siti Herlinda

Keyword(s):

Oryza Sativa ◽

Oryza Sativa L ◽

Rice Production ◽

Direct Seeding ◽

Type B ◽

Rice Varieties ◽

Main Plot ◽

Agronomic Characteristic ◽

Planting System ◽

Plot Design

ABSTRACT The efforts to develop and increase rice production in tidal land need appropriate technologies including adaptable superior varieties and efficient seed planting system. This study was aimed to measure the agronomic characteristic of the three rice varieties on the systems of in-row direct seeding and broadcast in tidal land. The experiment was carried out at type B of tidal land in Sidoharjo Village, Air Saleh Subdistrict, Banyuasin District, from January to April 2016. The experiment was conducted using split plot design with four replications. The main plot was seed planting system, consisted of in-row direct seeding system and broadcast system; whereas subplot was three rice varieties, consisted of Inpari 22, Inpara 4, and Ciherang. Result of LSD test on interaction showed that though the best growth was obtained from the combination of Inpari 22 and in-row direct seeding and very significant with the others, but its yield was not significant with the combination of Inpara 4 and in-row direct seeding. It was concluded that Inpari 22 and Inpara 4 with in-row direct seeding system showed better growth and higher yield than Inpari 22, Inpara 4 and Ciherang with broadcast system in tidal lands. Ciherang was not suitable in tidal land, while Inpari 22 was suitable. Keywords: broadcast system, in-row direct seeding system, production

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Research Status and Genetic Improvement of Important Characters in Purple Rice

Journal of Agriculture and Crops ◽

10.32861/jac.66.78.84 ◽

2020 ◽

Author(s):

Bo Peng ◽

Xin-Hua Huang ◽

Zi-Yue Liu ◽

Yue Gao ◽

Juan Peng ◽

...

Keyword(s):

Genetic Improvement ◽

Genetic Basis ◽

Agronomic Traits ◽

Oryza Sativa L ◽

High Yield ◽

Rice Seed ◽

Quality Traits ◽

Rice Varieties ◽

Related Quality ◽

Depth Study

Rice (Oryza sativa L.) is one of the most important food crops in the world. Purple rice, as a special rice with important nutritional and health functions, has important scientific significance and application value for genetic improvement of purple rice and breeding of new varieties through in-depth study on its agronomic and related quality traits and products processed, and analysis of its genetic basis. In this paper, the new progress of purple rice in agronomic traits, quality traits, environmental impacts on purple rice and genetic basis of purple rice seed coat in recent years were reviewed and analyzed, and the application prospects of purple rice processing products and purple gene in rice genetic improvement were also prospected. Thus, our results will provide important information and reference for breeding new purple rice varieties with good quality and high yield.

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Rapid and targeted introgression of fgr gene through marker-assisted backcrossing in rice (Oryza sativa L.)

Genome ◽

10.1139/gen-2017-0100 ◽

2017 ◽

Vol 60 (12) ◽

pp. 1045-1050 ◽

Cited By ~ 5

Author(s):

Acga Cheng ◽

Ismanizan Ismail ◽

Mohamad Osman ◽

Habibuddin Hashim ◽

Nur Samahah Mohd Zainual

Keyword(s):

Oryza Sativa ◽

Oryza Sativa L ◽

Cost Effective ◽

Yield Potential ◽

Recurrent Parent ◽

Rice Varieties ◽

Fragrant Rice ◽

Self Sufficiency ◽

Effective Manner ◽

Fgr Gene

While it is crucial for developing countries like Malaysia to achieve self-sufficiency in rice (Oryza sativa L.), it is equally critical to be able to produce high-quality rice, specifically fragrant rice, which demands are often met through importation. The present study was aimed at developing high-yielding fragrant rice, in a timely and cost-effective manner. A marker-assisted backcross (MABC) approach was optimised to introgress the fragrance gene (fgr) into two high-yielding Malaysian varieties, MR84 and MR219, within two years utilising less than 50 molecular markers. Coupled with phenotypic screening, one single foreground marker (fgr-SNP) and 48 background markers were selected and utilised, revealing recovery of at least 90% of recurrent parent genome (RPG) in merely two backcross generations. Collectively, the yield potential of the developed BC2F2 lines (BLs) was higher (P > 0.05) than the donor parent, MRQ74, and similar (P < 0.05) to both the recurrent parents, MR84 and MR219. In addition, some of the developed BLs showed good grain quality, such as having long grain. We believe that this is the first report comprising the validation and utilisation of the single functional marker system (fgr-SNP) in introgressing the fgr gene into different rice varieties.

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Respons Beberapa Varietas Padi (Oryza sativa L.) terhadap Wereng Batang Coklat (Nilaparvata lugens Stall) Koloni Karawang

Agrikultura ◽

10.24198/agrikultura.v29i2.19249 ◽

2018 ◽

Vol 29 (2) ◽

pp. 73

Author(s):

Dedi Darmadi ◽

Tuti Alawiyah

Keyword(s):

Oryza Sativa ◽

Rice Yield ◽

Oryza Sativa L ◽

Rice Variety ◽

Brown Planthopper ◽

Nilaparvata Lugens ◽

Test Method ◽

Rice Varieties ◽

Directorate General ◽

Population Origin

ABSTRACTResponse of rice varieties (Oryza sativa L.) to brown planthopper (Nilaparvata lugens Stall) of Karawang coloniesThe research was conducted to determine the resistant level of several rice varieties (Oryza sativa L.) against brown planthopper (Nilaparvata lugens Stall) Karawang colony. The experiment was done at the Entomology Laboratory of Pest Forcesting Institute, Jatisari, Directorate General of Food Crops, Ministry of Agriculture. It was conducted from February to June 2017. The resistant of rice varieties against brown planthopper was tested using honey dew test method with bromocressol green as indicator. Honey dew test method is one of methods to evaluate rice variety reaction against brown planthopper population origin of certain area. This is due to the production of honey dew by brown planthopper which positively correlated with the amount of liquid being drawn from the plant. This study used Duncan Test on 5% level. The result demonstrated the variation of rice resistant against brown planthopper based on the area of honey dew spot, brown planthover mortality, attacks intensity, the destruction level on plant, and the rice yield. Inpari 13 was the most toleranct rice variety against the infestation of brown planthopper of Karawang colony.Keywords: Brown planthopper, Honey dew, Paddy, Resistance, VarietiesABSTRAKPenelitian ini bertujuan untuk menilai tingkat ketahanan varietas padi (Oryza sativa L.) terhadap wereng batang coklat (Nilaparvata lugens Stall) koloni Karawang. Penilitian dilaksanakan di Laboratorium Entomologi Balai Besar Peramalan Organisme Pengganggu Tumbuhan (BBPOPT) Jatisari, Direktorat Jenderal Tanaman Pangan, Kementerian Pertanian. Penelitian berlangsung dari Bulan Februari sampai Bulan Juni 2017. Pengujian ketahanan varietas padi terhadap wereng batang coklat dilakukan dengan menggunakan metode uji embun madu (honeydew test) dengan memakai indicator bromocressol green. Metode uji embun madu merupakan salah satu metode untuk mengevaluasi reaksi suatu varietas terhadap populasi wereng batang coklat yang berkembang di suatu wilayah tertentu, karena jumlah embun madu yang dikeluarkan oleh wereng batang coklat mempunyai hubungan yang positif dengan jumlah cairan tanaman yang dihisap. Uji lanjut yang digunakan adalah uji lanjut Duncan pada taraf 5%. Hasil penelitian menunjukkan terdapat variasi ketahanan antar varietas padi yang diuji dilihat dari luas bercak embun madu yang dihasilkan pada masing-masing varietas padi, mortalitas wereng batang coklat, intensitas serangan, tingkat kerusakan tanaman, dan bobot hasil panen padi. BErdasarkan parameter tersebut di atas, Inpari 33 merupakan varietas padi yang tahan terhadap serangan wereng batang coklat koloni Karawang.Kata kunci : Embun madu, Ketahanan, Padi, Varietas, Wereng batang coklat

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Alokasi Biomassa pada Padi (Oryza sativa L.) Lokal Sulut Saat Kekurangan Air (Biomass Partitioning for North Sulawesi Local Rice (Oryza sativa L.) Cultivars under Water Deficit Condition)

JURNAL BIOS LOGOS ◽

10.35799/jbl.5.2.2015.10550 ◽

2015 ◽

Vol 5 (2) ◽

Author(s):

Song Ai Nio ◽

Audry Agatha Lenak ◽

Feky R Mantiri ◽

Susan M Mambu ◽

Daniel PM Ludong

Keyword(s):

Oryza Sativa ◽

Water Deficit ◽

Biomass Allocation ◽

Drought Resistance ◽

Oryza Sativa L ◽

Rice Production ◽

Biomass Partitioning ◽

Root:Shoot Ratio ◽

Rice Varieties ◽

North Sulawesi

ABSTRAK Salah satu masalah utama yang menjadi kendala dalam produksi padi di Indonesia adalah kekurangan air yang melanda areal persawahan sehingga menjadi suatu ancaman untuk produksi padi. Masalah tersebut dapat diatasi dengan beberapa cara dan salah satunya dapat dilakukan melalui strategi adaptasi. Oleh sebab itu kajian sifat tahan kering pada padi lokal Sulawesi Utara (Sulut) perlu dilakukan, dalam upaya mendukung tercapainya tujuan strategis tersebut. Penelitian ini dilakukan untuk mengevaluasi sifat tahan kering pada empat varietas padi lokal Sulut (Burungan, Superwin, Temo dan Ombong) pada fase vegetatif saat kekurangan air berdasarkan alokasi biomassa. Setelah 14 hari perlakuan, rasio akar:tajuk pada keempat varietas menunjukkan bahwa varietas Superwin, Temo dan Burungan yang tidak diairi air memiliki rasio akar:tajuk yang lebih besar daripada yang diairi. Berdasarkan alokasi biomassa, sifat tahan kering Superwin, Temo dan Ombong lebih besar dibandingkan dengan Burungan, sehingga varietas ini potensial untuk ditanam di daerah kekurangan air. Kata kunci: biomassa, kekeringan, padi lokal Sulut, vegetative ABSTRACT One of the main problems in rice production in Indonesia is the lack of water and this condition resulted in the decrease of rice production. The problem can be solved in several ways and one of them is adaptation strategies. This study was conducted to evaluate drought resistance in four North Sulawesi local rice varieties (Burungan, Superwin, Temo and Ombong) at the vegetative phase under water deficit based on biomass allocation. After 14 days of treatment, the root:shoot ratio of Superwin, Temo and Burungan under drought were greater than under well-watered condition. Based on the biomass allocation, the drought resistance of Superwin, Temo and Ombong were greater than Burungan, so that these varieties were potential to be grown in the water deficit areas. Keywords: biomass, drought, North Sulawesi local rice, vegetative

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Mutation Breeding of a N-methyl-N-nitrosourea (MNU)-Induced Rice (Oryza sativa L. ssp. Indica) Population for the Yield Attributing Traits

Sustainability ◽

10.3390/su11041062 ◽

2019 ◽

Vol 11 (4) ◽

pp. 1062 ◽

Cited By ~ 2

Author(s):

Tran Xuan ◽

Truong Anh ◽

Hoang-Dung Tran ◽

Tran Khanh ◽

Tran Dat

Keyword(s):

Agronomic Traits ◽

Amylose Content ◽

Oryza Sativa L ◽

Mutation Breeding ◽

Rice Production ◽

High Yield ◽

Rice Cultivars ◽

Induced Mutation ◽

Female Line ◽

Trait Locus

Difficulties in breeding new rice cultivars that have a high yield, are acceptable quality, and are tolerant to environmental stresses have been the major constraint of rice production in many developing countries, as these traits are determined by multiple genes associated with complicated and uncontrollable gene segregations.Furthermore, the gene/QTL (quantitative trait locus) introduced to the cultivar is unstable due to the interaction among the active genes, which determine the phenotypic performance, not yet been well understood or controllable. In this study, the N-methyl-N-nitrosourea (MNU)-induced mutation was applied to the heterozygote of the F1 generation from the cross between TBR1 (female) and KD18 (male parent). The phenotype and genotype of the M2 and M3 generations were evaluated and showed that the mutant population phenotypes, including the plant height, semi-dwarfism, amylose content, protein content, gel consistency, grain yield, and spikelet fertility, varied. Interestingly, no segregation among the genotypes in the M2 and M3 generations was observed, while the genotypes of the control population were either paternally inherited or indeterminable when using 28 polymorphism simple sequence repeat (SSR) markers that were identified on parental lines from 200 markers. The MNU-induced mutation caused maternal inheritance in the segregating populations, as primarily important agronomic traits were maternally succeeded from the female line TBR1. The findings of this study indicated that, through the use of MNU, the breeding of rice cultivars with close genetic backgrounds (similarity coefficient = 0.52) could be shortened by the maternal control of important qualities, such as pest and disease resistance and high yield, thus contributing to sustainable rice production for rice farmers. Further examination of rice cultivars with a greater difference in the genetic background should be subsequently conducted.

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