The Binding and Viscometric Studies of Ni+2, Co+2 and Mn+2 Ions with Protein by Spectrometric and pH Metric Techniques

2019 ◽  
Vol 9 (2) ◽  
pp. 151-162
Author(s):  
Shveta Acharya ◽  
Arun Kumar Sharma
Keyword(s):  
Metal Ions ◽  
Metal Ion ◽  
Protein Molecule ◽  
Vital Role ◽  
Sufficient Evidence ◽  
Structural Requirement ◽  
Nickel Ions ◽  
Cobalt Ions ◽  
Lower Temperature ◽  
Specific Oxidation

Background: The metal ions play a vital role in a large number of widely differing biological processes. Some of these processes are quite specific in their metal ion requirements. In that only certain metal ions, in specific oxidation states, can full fill the necessary catalytic or structural requirement, while other processes are much less specific. Objective: In this paper we report the binding of Mn (II), Ni (II) and Co (II) with albumin are reported employing spectrophotometric and pH metric method. In order to distinguish between ionic and colloidal linking, the binding of metal by using pH metric and viscometric methods and the result are discussed in terms of electrovalent and coordinate bonding. Methods: The binding of Ni+2, Co+2 and Mn+2 ions have been studied with egg protein at different pH values and temperatures by the spectrometric technique. Results: The binding data were found to be pH and temperature dependent. The intrinsic association constants (k) and the number of binding sites (n) were calculated from Scatchard plots and found to be at the maximum at lower pH and at lower temperatures. Therefore, a lower temperature and lower pH offered more sites in the protein molecule for interaction with these metal ions. Statistical effects seem to be more significant at lower Ni+2, Co+2 and Mn+2 ions concentrations, while at higher concentrations electrostatic effects and heterogeneity of sites are more significant. Conclusion: The pH metric as well as viscometric data provided sufficient evidence about the linking of cobalt, nickel and manganese ions with the nitrogen groups of albumin. From the nature and height of curves in the three cases it may be concluded that nickel ions bound strongly while the cobalt ions bound weakly.

scholarly journals Sorption of Pb2+, Cu2+ and Zn2+ from Aqueous Solution Using a Blended Membrane of Immobilized Karkashi (Sesame Leaves) and Sodium Alginate

2020 ◽  
pp. 21-29
Author(s):  
Ibrahim Maradona ◽  
J. H. Kanus ◽  
M. Suleman Stephen
Keyword(s):  
Aqueous Solution ◽  
Ionic Strength ◽  
Metal Ions ◽  
Sodium Alginate ◽  
Metal Ion ◽  
Vital Role ◽  
Ion Uptake ◽  
Ion Concentration ◽  
Sorption Behavior ◽  
Blended Membrane

Sorption techniques are widely used to remove heavy metal ions from large volume of aqueous solutions, this plays a vital role in controlling environmental pollution. Herein, the sorption of Pb2+, Cu2+ and Zn2+ with blended membrane of sodium alginate (Na-Alg) and immobilized karkashi leaves (IKAL) from aqueous solution was investigated at optimum conditions for effectiveness. Sorption capacities of the membrane for Pb2+, Cu2+ and Zn2+ were found to be 86.80, 40.60 and 39.55% respectively. The sorption behavior with respect to initial pH, ionic strength, temperature, contact time and initial metal ion concentration was investigated for optimum sorption conditions. Maximum sorption was found to occur at pH 3.0 for all the metal ions studied. Results showed that metallic ion uptake by the blended membrane declined with increase in ionic strength. This trend was also observed at an increased temperature of 50ºC for Pb2+, Cu2+ and 40ºC for Zn2+. Increase in initial metal ion concentration led to increase in metal ion uptake. At the end of the chemical remediation, the blended membrane was found to be effective to some extent, with the reaction being exothermic.

scholarly journals Development of Biopolymer and Conducting Polymer-Based Optical Sensors for Heavy Metal Ion Detection

Molecules ◽  
2020 ◽  
Vol 25 (11) ◽  
pp. 2548 ◽  
Author(s):  
Nur Syahira Md Ramdzan ◽  
Yap Wing Fen ◽  
Nur Ain Asyiqin Anas ◽  
Nur Alia Sheh Omar ◽  
Silvan Saleviter
Keyword(s):  
Heavy Metal ◽  
Metal Ions ◽  
Conducting Polymer ◽  
Metal Ion ◽  
Heavy Metal Ions ◽  
Polymer Materials ◽  
Heavy Metal Ion ◽  
Sufficient Evidence ◽  
Ion Detection ◽  
Metal Ion Detection

Great efforts have been devoted to the invention of environmental sensors as the amount of water pollution has increased in recent decades. Chitosan, cellulose and nanocrystalline cellulose are examples of biopolymers that have been intensively studied due to their potential applications, particularly as sensors. Furthermore, the rapid use of conducting polymer materials as a sensing layer in environmental monitoring has also been developed. Thus, the incorporation of biopolymer and conducting polymer materials with various methods has shown promising potential with sensitively and selectively toward heavy metal ions. In this feature paper, selected recent and updated investigations are reviewed on biopolymer and conducting polymer-based materials in sensors aimed at the detection of heavy metal ions by optical methods. This review intends to provide sufficient evidence of the potential of polymer-based materials as sensing layers, and future outlooks are considered in developing surface plasmon resonance as an excellent and valid sensor for heavy metal ion detection.

scholarly journals Adsorption of Copper, Zinc and Nickel Ions from Single and Binary Metal Ion Mixtures on to Chicken Feathers

2002 ◽  
Vol 20 (9) ◽  
pp. 849-864 ◽  
Author(s):  
Sameer Al-Asheh ◽  
Fawzi Banat ◽  
Dheaya‘ Al-Rousan
Keyword(s):  
Heavy Metal ◽  
Metal Ions ◽  
Metal Ion ◽  
Heavy Metal Ions ◽  
Batch Adsorption ◽  
Nickel Ions ◽  
Chicken Feathers ◽  
Binary Metal ◽  
Adsorption Processes ◽  
Copper Zinc

Certain industries often produce mixtures of heavy metal ions in their waste products. Because of the nature of heavy metal ions and the adsorption process, such metal ions can compete with each other for the sorption sites on an adsorbent during adsorption processes. In the present work, binary systems composed of copper, zinc and nickel ions were selected as examples of heavy metal ion mixtures and tested via batch adsorption processes using chicken feathers as an adsorbent. The uptake of individual metal ions was depressed by the presence of another. Thus, the uptake of copper ions from an initial copper ion solution of 20 ppm concentration was reduced from 0.042 mmol/g to ca. 0.019 mmol/g by the presence of a similar concentration of nickel ions. The Freundlich, Langmuir and Sips multi-component adsorption models were employed to predict the uptake of metal ions from binary metal ion solutions using constants obtained from adsorption isotherm models applied to single-solute systems.

scholarly journals A Multiuse Nanopore Platform with Disposable Paper Analytical Device for the Detection of Heavy Meatal Ions

2020 ◽  
Author(s):  
Imogen Heaton ◽  
Mark Platt
Keyword(s):  
Heavy Metal ◽  
Metal Ions ◽  
Surface Charge ◽  
Metal Ion ◽  
Heavy Metal Contamination ◽  
Low Cost ◽  
Analytical Techniques ◽  
Environmental Sensors ◽  
Nickel Ions ◽  
Ion Current Rectification

The pollution of heavy metal ions within the environmental is a global problem. The rapid and precise removal of these contaminants can be aided by identifying and quantifying the composition of the sample. It is therefore crucial to develop effective portable analytical techniques to determine the levels of heavy metal contamination. Paper-based analytical devices (PADs) offer a low cost method making them an excellent platform for onsite environmental sensors. Here we demonstrate how a PAD can be integrated into a multi-use Nanopore platform. The PAD was functionalised with different recognition ligands, who’s surface charge densities varied in the presence of an analyte. The surface of the PAD was placed in contact with a Nanopore which exhibited Ion Current Rectification (ICR). The extent of ICR, was dependent upon the PAD’s surface charge, and the presence of the analyte of interest i.e. the ICR phenomena was exaggerated or diminished indicating the presence of the metal ion in solution. We demonstrate the potential of PAD-ICR using a PAD functionalised with a peptide aptamer specific for nickel ions. Allowing the detection of nickel(II) as low as 0.25 μM even in the presence of other metal ions. After any measurement, the Nanopore surface can be wiped clean, and reused.

scholarly journals A Multiuse Nanopore Platform with Disposable Paper Analytical Device for the Detection of Heavy Meatal Ions

2020 ◽  
Author(s):  
Imogen Heaton ◽  
Mark Platt
Keyword(s):  
Heavy Metal ◽  
Metal Ions ◽  
Surface Charge ◽  
Metal Ion ◽  
Heavy Metal Contamination ◽  
Low Cost ◽  
Analytical Techniques ◽  
Environmental Sensors ◽  
Nickel Ions ◽  
Ion Current Rectification

The pollution of heavy metal ions within the environmental is a global problem. The rapid and precise removal of these contaminants can be aided by identifying and quantifying the composition of the sample. It is therefore crucial to develop effective portable analytical techniques to determine the levels of heavy metal contamination. Paper-based analytical devices (PADs) offer a low cost method making them an excellent platform for onsite environmental sensors. Here we demonstrate how a PAD can be integrated into a multi-use Nanopore platform. The PAD was functionalised with different recognition ligands, who’s surface charge densities varied in the presence of an analyte. The surface of the PAD was placed in contact with a Nanopore which exhibited Ion Current Rectification (ICR). The extent of ICR, was dependent upon the PAD’s surface charge, and the presence of the analyte of interest i.e. the ICR phenomena was exaggerated or diminished indicating the presence of the metal ion in solution. We demonstrate the potential of PAD-ICR using a PAD functionalised with a peptide aptamer specific for nickel ions. Allowing the detection of nickel(II) as low as 0.25 μM even in the presence of other metal ions. After any measurement, the Nanopore surface can be wiped clean, and reused.

The Thermodynamic and pH Metric Binding Studies of Cu+2 Ions with Egg Protein by Spectrometric and Diffusion Current Techniques

2020 ◽  
Vol 234 (3) ◽  
pp. 441-460 ◽  
Author(s):  
Shveta Acharya ◽  
Arun Kumar Sharma
Keyword(s):  
Metal Ion ◽  
Copper Ions ◽  
Protein Molecule ◽  
Living System ◽  
Diffusion Current ◽  
Binding Studies ◽  
Spectrophotometric Methods ◽  
Egg Protein ◽  
Physico Chemical ◽  
Lower Temperature

AbstractTransition metals have unique efficacy in catalyzing various industrial reactions and also in living system, the redox reaction and redox changes in the metal ions catalyzed valence changes in the substrate molecule. The survey of the existing literature revealed that the binding of Molybdenum, Vanadium, Zinc, Cadmium, Copper, Nickel and Cobalt with the protein is well known but no binding studies of copper metal with egg protein are reported. With a view to extend the existing knowledge of ecological nature of metal-protein system, it was thought of interest to investigate the properties of metal-protein mixture. Investigations on the aspects of these binding problems were planned and their bindings constants have been determined using suitable physico-chemical methods. The pH metric, diffusion current measurements, spectrophotometric methods have been used on the binding of copper ions with albumin. The effect of physico-chemical factors on interaction between divalent metal ion i.e. copper with albumin has been discussed. On the basis of observed results, it is found that the binding data were dependent on pH and temperature. From scatchard plots, the intrinsic association constants (k) and the number of binding sites (n) were calculated and found high at lower pH and temperatures. Therefore, a lower temperature and lower pH offered more sites in the protein molecule for interaction with copper (II) ions. The enthalpy (ΔH), entropy (ΔS) changes, free energy change (ΔG°) have been calculated.

scholarly journals Metal Release of Standard and Fake Orthodontic Braces: An In Vitro Study

2021 ◽  
Vol 20 (2) ◽  
Author(s):  
Siti Hajjar Nasir ◽  
Muhammad Syahmi Mohamad Amran ◽  
Muhammad Muaz Abidin Mustaffar
Keyword(s):  
Metal Ions ◽  
Inductively Coupled Plasma ◽  
Metal Ion ◽  
Artificial Saliva ◽  
In Vitro Study ◽  
Nickel Ions ◽  
Inductively Coupled ◽  
Significant Difference ◽  
Plasma Mass Spectrometry

INTRODUCTION: The growing demand for orthodontic braces among Malaysians has led to the development of fake braces. These fake braces services are illegal and their brackets are reported to be of inferior quality. Fake braces are constantly being exposed to the saliva intraorally, hence they are susceptible to corrosion. This study was conducted to investigate the release of metal ions as a result of corrosion from standard and fake orthodontic braces immersed in artificial saliva of different pH. MATERIALS AND METHODS: A total of six different types of brackets (three from standard and three from fake braces) were immersed in containers containing 5 mL of artificial saliva of pH 4.9 and pH 7.8. The samples were collected for analysis on day 1, day 14, and day 28 using Inductively-Coupled Plasma Mass Spectrometry (ICPMS) to evaluate the amount of metal ion released. Statistical analysis was performed to isolate the significant difference of metal ions released between two types of braces in different pH solutions. RESULTS: The release of aluminum, nickel, chromium, manganese and copper were observed and analyzed. Fake braces released the highest concentration of chromium, manganese, and nickel ions in both artificial saliva as compared to standard braces. Brackets immersed in pH 4.9 released a higher number of ions compared to pH 7.8. CONCLUSION: This study showed that fake braces released the highest concentration of metal ions as compared to standard braces. Both time and pH influenced the release of metal ions from orthodontic brackets.

Use of agro-waste (Musa paradisiaca peels) as a sustainable biosorbent for toxic metal ions removal from contaminated water

2019 ◽  
Author(s):  
Chem Int
Keyword(s):  
Heavy Metal ◽  
Metal Ions ◽  
Contact Time ◽  
Metal Ion ◽  
Equilibrium Concentration ◽  
Ion Concentration ◽  
Contaminated Water ◽  
Musa Paradisiaca ◽  
Percentage Removal ◽  
Adsorbent Dose

A study of removal of heavy metal ions from heavy metal contaminated water using agro-waste was carried out with Musa paradisiaca peels as test adsorbent. The study was carried by adding known quantities of lead (II) ions and cadmium (II) ions each and respectively into specific volume of water and adding specific dose of the test adsorbent into the heavy metal ion solution, and the mixture was agitated for a specific period of time and then the concentration of the metal ion remaining in the solution was determined with Perkin Elmer Atomic absorption spectrophotometer model 2380. The effect of contact time, initial adsorbate concentration, adsorbent dose, pH and temperature were considered. From the effect of contact time results equilibrium concentration was established at 60minutes. The percentage removal of these metal ions studied, were all above 90%. Adsorption and percentage removal of Pb2+ and Cd2+ from their aqueous solutions were affected by change in initial metal ion concentration, adsorbent dose pH and temperature. Adsorption isotherm studies confirmed the adsorption of the metal ions on the test adsorbent with good mathematical fits into Langmuir and Freundlich adsorption isotherms. Regression correlation (R2) values of the isotherm plots are all positive (>0.9), which suggests too, that the adsorption fitted into the isotherms considered.

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