scholarly journals Preparation of Some Culture Media Locally from leaves and stems of Purslane plant (Portulaca oleracea oleracea L.) and Assessment of Their Efficiency Comparing with Culture Media of Oxoid Company

2015 ◽  
Vol 12 (3) ◽  
pp. 459-467
Author(s):  
Baghdad Science Journal
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Staphylococcus Epidermidis ◽  
Pathogenic Bacteria ◽  
Culture Media ◽  
Portulaca Oleracea ◽  
Good Growth ◽  
Proteus Vulgaris ◽  
Selective Media ◽  
The Media

The leaves and stems of the local Purslane plant ( Portulaca oleracea oleracea L. ) were used to preapare the extract of two types ( wet and dried extractions) the extracts were prepared by weighting of 60grams of the wet and the dried plant individually, then boiled in 500ml of distal water. Finally the volume was completed to1 liter, then we used these extracts to prepare of 8 types of the culture media contained basic, selective and enrichment media for growing a group of pathogenic bacteria. 8 types of bacteria were used for this purpose: Escherichia coli, Pseudomonas flouresence, Staphylococcus aureus , Staphylococcus epidermidis, Bacillus subtilis , Klebsiella pneumoniae , Proteus mirabilis and Proteus vulgaris. The stastical analysis of the results showed that the locally prepared culture media in this research (Nutrient wet portulaca agar , Blood - wet portulaca agar, Manitol- wet portulaca agar, Lactose- wet portulaca agar, Nutrient dried portulaca agar , Blood - dried portulaca agar , Manitol- dried portulaca agar and Lactose- dried portulaca agar) were suitable for culturing of these pathogenic bacteria when compared to the media that supplied by Oxoid company. In addition they also revealed good growth and they were sufficient to use as basic, enriched and selective media .

scholarly journals Susceptibility patterns of bacteria isolated from the hospital environment towards disinfectants commonly used for surfaces and medical devices

2021 ◽  
Vol 319 ◽  
pp. 01081
Author(s):  
Samira Jaouhar ◽  
Abdelhakim El Ouali Lalami ◽  
Jawad Bouzid ◽  
Ikrame Zeouk ◽  
Khadija Bekhti
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Medical Devices ◽  
Staphylococcus Epidermidis ◽  
Pathogenic Bacteria ◽  
Hospital Environment ◽  
Gram Positive ◽  
Microdilution Method ◽  
Bacillus Spp ◽  
Didecyldimethylammonium Chloride

This study aimed to evaluate the bactericidal activity of common disinfectants used for surfaces and medical devices. Sodium hypochlorite (D1), disinfectant (D2) composed of N-(3-aminopropyl)-N-dodecylpropane-1,3-diamine, chloride de didecyldimethylammonium, and disinfectant (D3) composed of Didecyldimethylammonium chloride and Polyhexamethylene biguanide hydrochloride, were tested against 15 strains isolated from the hospital environment and four reference bacteria. The microdilution method was performed to assess antimicrobial activity. The susceptibility was evaluated by comparing the minimum inhibitory dilution with the dilution of disinfectant recommended by the manufacture. D1 and D2 were active against Staphylococcus epidermidis, Staphylococcus saprophyticus, Enterobacter cloacae, Escherichia coli, Pseudomonas fluorescens, Methicillin-resistant Staphylococcus aureus, Bacillus spp, Corynebacterium spp, Gram-positive bacillus, Escherichia coli ATCC 25922, Bacillus subtilis ATCC 3366, and Pseudomonas aeruginosa ATCC 27853 strains but not active against Micrococcus spp, and Staphylococcus aureus ATCC 29213. D3 was ineffective against Micrococcus spp, Bacillus Gram Positive, Staphylococcus epidermidis, and Escherichia coli ATCC 25922. Therefore, D1 and D2 can eliminate most pathogenic bacteria in hospitals, in comparison to D3. It is necessary to monitor the antibacterial activity of disinfectants against reference strains but also against those usually present on surfaces. The obtained results could have promising applications in controlling the emergence of nosocomial infections.

scholarly journals Study the Antimicrobial effect of Kombucha tea on bacteria isolated from Diabetic foot ulcer

2014 ◽  
Vol 8 (4) ◽  
pp. 27-33
Author(s):  
E. J. AL-Kalifawi
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Klebsiella Pneumoniae ◽  
Enterococcus Faecalis ◽  
Proteus Mirabilis ◽  
Staphylococcus Epidermidis ◽  
Enterobacter Cloacae ◽  
Proteus Vulgaris ◽  
Kombucha Tea

The study was conducted from January to March 2012. In this study colorimetric VITEK-2 Compact system used for its accuracy and rapidity to identify isolates and to detect several antimicrobial resistances.The study also investigate the antibacterial effect of Kombucha tea on isolated bacteria from diabetic foot ulcer. The bacteria isolated were eight gram negative bacteria, namely, Acinetobacter baumannii 3 (2%), Enterobacter cloacae 5 (4%), Escherichia coli 13 (10%), Klebsiella pneumoniae 7 (6%), Citrobacter spp. 4 (3%), Proteus mirabilis 3 (2%), Proteus vulgaris 3 (2%) and Pseudomonas aeruginosa 44 (35%). Four gram positive bacterium, Enterococcus faecalis 6 (5%), Staphylococcus aureus 17 (13%), Staphylococcus epidermidis 13 (10%) and Streptococcus spp. 9 (8%). The antimicrobial activities of antibiotics showed that, all isolates are sensitive to Ciprofloxacin, Levofloxacin and Ofloxacin. The resistance to other types of antimicrobial differ with different isolate. The effect of Kombucha tea on all isolates wasclear at 7days of incubation; the diameter of inhibition was 6mm for Acinetobacter baumannii, Proteus vulgaris and Enterococcus faecalis. 7mm for Enterobacter cloacae, Escherichia coli, Klebsiella pneumoniae, Citrobacter spp., Staphylococcus epidermidis and Streptococcus spp. 8mm for Proteus mirabilis, Pseudomonas aeruginosa and Staphylococcus aureus. The maximum activity of fermented tea was recorded at 14days incubation of Kombucha organism against all isolates, the diameter of inhibition was 21mm for Acinetobacter baumannii, 24mm for Enterobacter cloacae, 23mm for Escherichia coli, Staphylococcus epidermidis and Streptococcus spp., 16mm for Klebsiella pneumoniae, 22mm for Citrobacter spp. and Enterococcus faecalis, 25mm for Proteus mirabilis and Staphylococcus aureus, 20mm for Proteus vulgaris, 26mm for Pseudomonas aeruginosa. The antibacterial activity of Kombucha tea decrease with increase incubation periods28 days.

scholarly journals Influence of some antibiotics and sulfonamides on the microbiological background of the Baikal seal

2020 ◽  
Vol 5 (4) ◽  
pp. 364-373
Author(s):  
Anna A. Pliska ◽  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Enterococcus Faecalis ◽  
Clostridium Perfringens ◽  
Staphylococcus Epidermidis ◽  
Proteus Vulgaris ◽  
Staphylococcus Warneri ◽  
Treatment Measures

The results of microbiological studies of the material taken from the diseased seal are presented. The taxonomic characteristics of microorganisms, their virulence and antibiotic resistance were studied. Treatment measures were developed and performed. It was found that the seal has the following types of microorganisms: Staphylococcus aureus; Enterococcus faecalis; Escherichia coli; Staphylococcus warneri; Proteus vulgaris; Clostridium perfringens, Proteushauseri, Staphylococcus epidermidis, Plesiomonas shigel-loides. All pathogens isolated from the seals were sensitive to 60.3% of antibiotics (ampicillin, cefuroxime, cefixime, gentamicin, amikacin, ciprofloxacin, ofloxacin, furadonin, Ceftriaxone, vancomycin) and resistant to 39.7% of antibiotics, two representatives of cephalosporins (cefaclor and ceftazidim) and ticarcillin.

scholarly journals Circulating microflora and its resistance to antibacterial medications in the conditions of Farm “Pchani-Denkovich” of Zhydachiv district of Lviv region

2018 ◽  
Vol 20 (92) ◽  
pp. 13-17
Author(s):  
Ya. V. Kisera ◽  
L. Ya. Bozhyk ◽  
Yu. G. Storchak
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Staphylococcus Epidermidis ◽  
Enterobacter Cloacae ◽  
Proteus Vulgaris ◽  
Antibacterial Drugs ◽  
Milk Samples ◽  
Enterococcus Spp ◽  
Number Of Microorganisms ◽  
Nutrient Environment

The importance of the influence of microorganisms on the health of humans and animals is beyond doubt. In recent decades, a large amount of data on the interaction of the flora with the elements of the immune system has been accumulated. Therefore, it is important to identify any hazardous factors that must be prevented or neutralized. One of such factors is the circulating flora of the premises, the organism of the animal, its virulence and resistance to antibacterial drugs. Bacteriological studies included bacteriological culture on the nutrient environment, their identification and the study of antibioticsensitivity. According to the results of bacteriological studies of milk samples, it was found that in 25% of the studied samples Staphylococcus aureus cultures were found in different concentrations. In 4 samples, Proteus vulgaris was detected. Bacteriological studies of vaginal exudate from the cows after calving have shown that they have Escherichia coli, Escherichia coli haemolitica, Staphylococcus aureus, Streptococcus haemolyticus, Staphylococcus epidermidis, Enterobacter cloacae, Proteus vulgaris, mold fungi of the Aspergillus spp. The studies of the exudate from the nasal passages and the mouthof the calves found that all the tested samples contained Escherichia coli, Proteus vulgaris, Staphylococcus epidermidis, Enterococcus spp., Klebsiella pnemoniae and Aspergillus and Candida fungi. The results of calf excrement studies revealed the presence of a number of microorganisms: Escherichia coli, Escherichia coli haemolitica, Proteus vulgaris, Staphylococcus epidermidis, Enterobacter cloacae and Enterobacter faecalis in different percentages. In determining the sensitivity of isolated cultures to antibacterial drugs, it has been established that Staphylococcus aureus cultures are sensitive to all antibiotics; Proteus vulgaris show resistance to ampicillin, amoxicillin; Escherichia coli haemolytica is resistant to ampicillin.

scholarly journals SKIRTINGOSE LIETUVOS VIETOVĖSE SURINKTŲ ŽIEDADULKIŲ EKSTRAKTO ANTIMIKROBINIS VEIKIMAS

2021 ◽  
Vol 31 (7) ◽  
pp. 86-90
Author(s):  
Žaneta Maželienė ◽  
Gintarė Lingytė ◽  
Evelina Dailidaitė ◽  
Ingrida Viliušienė
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Candida Albicans ◽  
Listeria Monocytogenes ◽  
Klebsiella Pneumoniae ◽  
Enterococcus Faecalis ◽  
Staphylococcus Epidermidis ◽  
Proteus Vulgaris ◽  
Microsoft Excel

Įvadas. Pasaulio sveikatos organizacijos (PSO) duomenimis, bakterijų atsparumas antibiotikams kelia didelę grėsmę visame pasaulyje ir sunkina infekcinių ligų gydymą [1]. Manoma, kad apie 70 proc. bakterijų, kurios sukelia infekcinius susirgimus ligoninėse, yra atsparios bent vienam iš gydymui naudojamų antibiotikų. Ši situacija skatina ieškoti kitų sprendimų, vienas iš jų – bičių produktus naudoti kaip natūralius antibiotikus dėl jų antimikrobinio veikimo. Tyrimo tikslas – nustatyti skirtingose Lietuvos vietovėse surinktų žiedadulkių ekstraktų antimikrobinį veikimą. Tyrimo metodika. Atliktas įvairiuose Lietuvos ūkiuose surinktų žiedadulkių skirtingos koncentracijos ekstraktų mikrobiologinis tyrimas. Nustatytas 50 žiedadulkių ekstraktų mėginių antimikrobinis poveikis dešimties etaloninių bakterijų kultūrų ir grybelio Candida albicans atžvilgiu. Tyrimo duomenų analizė atlikta naudojant aprašomąją statistiką, skaičiuojant dažnius bei grupių palyginimą pagal Kruskal Wallis, tyrimo duomenys apdoroti Microsoft Excel 2019 programa. Tyrimo rezultatai. Nustatyta, kad stipriausią antimikrobinį poveikį turėjo D žiedadulkių, surinktų Kaišiadorių rajone, ekstraktas. D ekstrakto antimikrobinis poveikis Staphylococcus epidermidis, Pseudomonas aeruginosa, Bacillus cereus, Listeria monocytogenes atžvilgiu nustatytas esant 0,025 ml ekstrakto 1 agaro ml. Staphylococcus aureus, Enterococcus faecalis, Klebsiella pneumoniae, Proteus vulgaris ir grybelį Candida albicans, išskyrus Escherichia coli, ekstraktas veikė esant jo 0,0375 – 0,05 ml agaro 1 ml. Žiedadulkių ekstraktas gramteigiamąsias bakterijas veikia stipriau, lyginant su gramneigiamosiomis. Šis skirtumas siejamas su gramteigiamų ir gramneigiamų bakterijų ląstelės sienelės struktūros skirtumais. Išvados. Stipriausią antimikrobinį veikimą turi žiedadulkės, surinktos Kaišiadorių rajone. Nustatyta, kad žiedadulkių ekstraktai stipriau veikia gramteigiamąsias bakterijas, lyginant su gramneigiamosiomis.

scholarly journals Taxonomic characteristics of micro-organisms isolated from the Baikal seal

2021 ◽  
Vol 6 (1) ◽  
pp. 42-49
Author(s):  
A. A. Pliska
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Clostridium Perfringens ◽  
Staphylococcus Epidermidis ◽  
Enterobacter Cloacae ◽  
Laboratory Animals ◽  
Ecological Problem ◽  
Proteus Vulgaris ◽  
Proteus Hauseri ◽  
Micro Organisms

The relevance of the ecological problem of the mass death of the Baikal seal (Pusa sibirica Gm.) forces researchers to establish causes of this phenomenon. The article presents the results of microbiological studies of swabs isolated from the nasal, oral cavities and rectum. The taxonomic characteristics of microorganisms and their virulence were studied. 14 Baikal seals found in the nerpinaria of Listvyanka were tudied. It was established that in the seal there are the following microorganisms: Clostridium perfringens, Escherichia coli, Enterococcus faecalis, Staphylococcus warneri, Pseudomona aeruginosa, Staphylococcus epidermidis, Staphylococcus aureus, Proteus hauseri, Proteus vulgaris bellisella, Proteus migarisella, rabitemorganii, Psychrobactersanguinis, Enterobacter cloacae. When studying the virulence of microorganisms, it was found that 58% of the strains do not cause the death of laboratory animals, they are mainly the representatives of opportunistic microflora. 42% of the strain were pathogenic, of which two strains of Plesi omonas shigelloides and 42 strains of Clostridium perfringens were identified.

scholarly journals A Study on the Nature of Association betweenDemodexMites and Bacteria Involved in Skin and Meibomian Gland Lesions of Demodectic Mange in Cattle

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Mukhtar Taha Abu-Samra ◽  
Yassir Adam Shuaib
Keyword(s):  
Staphylococcus Aureus ◽  
Streptococcus Pyogenes ◽  
Staphylococcus Epidermidis ◽  
Pathogenic Bacteria ◽  
Skin Lesions ◽  
Meibomian Gland ◽  
Proteus Vulgaris ◽  
High Turnover ◽  
Opportunistic Bacteria ◽  
Group A

The nature of association betweenDemodexmites and bacteria involved in bovine demodectic mange lesions and the normal flora inhabiting the skin of noninfected animals was investigated.Demodex bovisandD. ghanensismites were isolated from the infected purulent material extracted from skin and meibomian gland lesions, respectively. The mites could not be demonstrated in skin brushings or impression smears from the eyes of noninfected cattle. Pathogenic bacteria (Staphylococcus aureusandStreptococcus pyogenes(Group A)) and opportunistic organisms (Proteus vulgaris,Pseudomonas aeruginosa,Staphylococcus epidermidis, andTrueperella pyogenes) were isolated from skin lesions of demodectic mange, andMoraxella bovisandStaphylococcus aureuswere isolated from meibomian gland lesions.Bacillus subtilis,Escherichia coli,Proteus vulgaris,Staphylococcus aureus,Staphylococcus epidermidis, andStreptococcus pyogenes(Group A) were isolated from skin brushings from noninfected cattle. The nature of association betweenDemodexmites and bacteria in demodectic mange lesions is synergistic and of equal significance. Pathogenic and opportunistic bacteria facilitated the establishment ofDemodexmites in the lesions produced and provided an excellent microclimate for the mites to propagate and reproduce, resulting in severe and progressive disease. The “high-turnover” granulomatous reaction which characterized the histopathological changes proved thatDemodexmites and associated bacteria were persistent and immunogenic.

scholarly journals Genetic diversity of mastitis cow’s milk bacteria based on RAPD-PCR

2018 ◽  
Vol 19 (5) ◽  
pp. 1714-1721 ◽  
Author(s):  
APON ZAENAL MUSTOPA ◽  
INES FERDIANA PUSPITASARI ◽  
FATIMAH FATIMAH ◽  
LITA TRIRATNA ◽  
GINA KARTINA
Keyword(s):  
Escherichia Coli ◽  
Genetic Diversity ◽  
Staphylococcus Aureus ◽  
16S Rrna ◽  
Pathogenic Bacteria ◽  
Cow’S Milk ◽  
Pcr Analysis ◽  
Selective Media ◽  
Cow's Milk ◽  
Rapd Pcr

Mustopa AZ, Puspitasari IF, Fatimah, Triratna L, Kartina G. 2018. Genetic diversity of mastitis cow’s milk bacteria based onRAPD-PCR. Biodiversitas 19: 1714-1721. Mastitis in cow is caused by several pathogenic bacteria, including antibiotic-resistantbacteria. Identification of the pathogenic bacteria’s diversity that is contaminating cow’s milk needs to be done. The aim of this researchwas to conduct molecular identification in mastitis cow’s milk bacteria through RAPD-PCR (Random Amplified Polymorphism DNAPolymeraseChain Reaction) analysis. Bacteria from mastitis cow’s milk were enumerated using selective media. Based on the result ofmedia selection, there were 72 isolates of bacteria from mastitis cows in Ciguha, Guranteng, Cikarenceng Village (Pagerageung,Tasikmalaya), and Warnasari Village (Pangalengan, Bandung), West Java, Indonesia. The genomes from these isolates were extractedand then subjected to RAPD-PCR analysis. The results of RAPD-PCR analysis showed 8 clusters of dendrogram which 4 dominantclusters were selected. Identification of 4 dominant clusters, which contained representative strains, using 16s rRNA showed the isolatesBPA-12, MHA-6, L-4, and XLDA-8 were identified as Staphylococcus aureus, Escherichia coli, Listeria monocytogenes, andEnterobacter bugandensis.

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