scholarly journals Effect of alcoholic extract of Adhatoda vasica leaves on some biological performance of Ceratitis capitata larvae

2012 ◽  
Vol 9 (1) ◽  
pp. 1-6
Author(s):  
Baghdad Science Journal
Keyword(s):  
Relative Humidity ◽  
Ceratitis Capitata ◽  
Fruit Fly ◽  
Biological Characteristics ◽  
Alcoholic Extract ◽  
Adhatoda Vasica ◽  
Laboratory Conditions ◽  
Biological Tests ◽  
Biological Performance

Many biological tests were done to study the effect of the crude alcoholic leaves extracts on the biological characteristics at the larvae fruit fly Ceratitis capitata with different concentrations of the extracts exposed 1,2,3,5 and 7.5 % at constant laboratory conditions includes the temperature and the relative humidity (27±2C0&70±5 % respectively). The results of the experiments showed that the alcoholic extracts of the plant effected the growth and the development of the larvae and pupae according to the concentrations . The alcoholic extracts proved to have the highest mortality tend to 66.76 % in larva treated with alcoholic at concentration 7.5 % the result also showed that, there was no signifecant differences on the perid of larva pupation that treated with the different concentration.

scholarly journals Oviposition of fruit flies (Diptera: Tephritidae) and its relation with the pericarp of citrus fruits

2017 ◽  
Vol 78 (3) ◽  
pp. 443-448 ◽  
Author(s):  
N. P. Dias ◽  
D. E. Nava ◽  
M. S. Garcia ◽  
F. F. Silva ◽  
R. A. Valgas
Keyword(s):  
Relative Humidity ◽  
Citrus Sinensis ◽  
Ceratitis Capitata ◽  
Fruit Fly ◽  
Fruit Flies ◽  
Citrus Fruits ◽  
Anastrepha Fraterculus ◽  
Biological Studies ◽  
Fruit Growing ◽  
The Relationship

Abstract Fruit flies (Diptera: Tephritidae) represent a threat to fruit growing worldwide, mainly the citrus culture, however, biological studies show that fruit flies are not perfectly adapted to this host. This study investigated oviposition of Anastrepha fraterculus (Wiedemann, 1830) and Ceratitis capitata (Wiedemann, 1824) and its relation with the pericarp of citrus fruits. We evaluated the relationship between depth of oviposition of A. fraterculus and C. capitata and epicarp thickness of orange [Citrus sinensis (L.) Osbeck)] ‘Navelina’ and tangerine [C. reticulata (L.)] ‘Clemenules’ and the influence of fruit mesocarp of tangerine ‘Clemenules’ on oviposition of these species. The study was conducted under controlled conditions of temperature (25 ± 2 °C), relative humidity (70 ± 10% RH) and photophase (12 h). A. fraterculus and C. capitata laid their eggs in the flavedo region of orange ‘Navelina’ and between the albedo and flavedo of tangerine ‘Clemenules’. When fruits with mesocarp exposed were offered, there was no oviposition by both fruit fly species. The results show that epicarp thickness of citrus fruits did not influence oviposition of A. fraterculus and C. capitata as oviposition did not occur only in the presence of the mesocarp, suggesting that other factors are involved in oviposition of these species.

scholarly journals Películas de partículas minerais sobre a oviposição da mosca-do-mediterrâneo em laboratório

2017 ◽  
Vol 10 (3) ◽  
pp. 183-186
Author(s):  
Cláudia Bernardes Ourique ◽  
Luiza Rodrigues Redaelli ◽  
Caio Fábio Stoffel Efrom ◽  
Douglas Pedrini
Keyword(s):  
Laboratory Tests ◽  
Ceratitis Capitata ◽  
Fruit Fly ◽  
Mediterranean Fruit Fly ◽  
Mineral Particle ◽  
Laboratory Conditions ◽  
The Mean ◽  
Product Water

Resumo. Com o intuito de testar a tecnologia de película de partículas minerais com produtos existentes no mercado brasileiro, este trabalho avaliou a ação de caulim e calcário líquido na oviposição de Ceratitis capitata (Wiedemann) (Diptera: Tephritidae). Foram realizados ensaios de laboratório com maçãs e mangas, onde os frutos foram submetidos a pulverizações com caulim e calcário líquido (20% produto + água + espalhante adesivo Break Thru®) e controles (água e água + o espalhante adesivo) e expostos, individualmente, em gaiolas contendo dez fêmeas de C. capitata, alimento e água. Após 24 horas, os frutos foram removidos das gaiolas e decorridas mais 24 horas, removia-se a película mineral e contava-se as o número de puncturas nestes. A média de puncturas registradas nos frutos dos controles, em maçãs e em mangas foi maior do que a dos frutos tratados. Caulim e calcário líquido não diferiram entre si nos dois experimentos.  Em condições de laboratório, os produtos testados impediram a oviposição das moscas, reduzindo a infestação nos frutos.Minerals particles films on mediterranean fruit fly oviposition in laboratory assaysAbstract. In order to test the technology of mineral particle film with products existing in the Brazilian market, this work evaluate the action of kaolin and limestone liquid on Ceratitis capitata (Wiedemann) (Diptera: Tephritidae) oviposition. Laboratory tests were carried out with apples and mangoes, where the fruits were sprayed with kaolin and liquid limestone (20% product + water + Break Thru ® adhesive) and controls (water and water + adhesive spreader) and individually exposed in cages containing ten C. capitata females, food and water. After 24 hours, the fruits were removed from the cages and after another 24 hours, the mineral film was removed and the number of punctures counted therein. The mean number of punctures recorded in the fruits of controls, apples and mangoes was higher than that of treated fruits. Kaolin and liquid limestone did not differ between the two experiments. Under laboratory conditions, the tested products prevented the oviposition of the flies, reducing infestation in the fruits.

scholarly journals Evaluating the repellent effect of four botanicals against two Bactrocera species on mangoes

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e8537 ◽  
Author(s):  
Waqar Jaleel ◽  
Desen Wang ◽  
Yanyuan Lei ◽  
Guojun Qi ◽  
Ting Chen ◽  
...  
Keyword(s):  
Relative Humidity ◽  
Natural Enemies ◽  
Azadirachta Indica ◽  
Fruit Fly ◽  
Bactrocera Dorsalis ◽  
Piper Nigrum ◽  
Repellent Effect ◽  
Laboratory Conditions ◽  
Number Of Visits ◽  
Harmful Effects

Background Bactrocera dorsalis and B. correcta are economically important fruit fly pests of crops, vegetables, fruits, and nuts worldwide, especially in China. Nowadays in China, B. correcta is a second notorious pest of many fruits after B. dorsalis. Different botanicals have been tested against the B. dorsalis but in the case of B. correcta, no records were published. Methodology This study evaluated the repellency of four botanicals (Seriphidium brevifolium, Piper nigrum, Azadirachta indica and quercetin) in acetone dilutions (5%, 2.5% and 1%) against the B. dorsalis and B. correcta at the laboratory conditions (25 ± 2 °C, 60 ± 5% relative humidity, and a photoperiod of L:D 14:10 h). Results The number of visits after 24–48 h, oviposition punctures, and pupae made by both species were lower on the treated mangoes in comparison to untreated mangoes. S. brevifolium, P. nigrum, A. indica and quercetin have significantly reduced the visits, ovipositional punctures, and pupae of both species. Among botanicals, the P. nigrum was the most effective repellent against B. correcta and as well as B. dorsalis. However, the harmful effects of these botanicals against natural enemies are still unknown.

scholarly journals Pathogenicity of indigenous Beauveria bassiana (Balsamo) against Ceratitis capitata Wiedemann (Diptera: Tephritidae) under laboratory conditions

2020 ◽  
Vol 30 (1) ◽  
Author(s):  
Sabrina Chergui ◽  
Khadidja Boudjemaa ◽  
Abdelmadjid Benzehra ◽  
Ismail Karaca
Keyword(s):  
Beauveria Bassiana ◽  
Filter Paper ◽  
Ceratitis Capitata ◽  
Insect Pest ◽  
Fruit Fly ◽  
Fruit Production ◽  
Main Body ◽  
Adult Males ◽  
Pesticides Residues ◽  
Laboratory Conditions

Abstract Background The Mediterranean fruit fly, Ceratitis capitata (Wiedemann) (Diptera: Tephritidae), is the major insect pest of fruit production worldwide. Chemical control of this pest has induced the selection of resistant medflies populations and negative environmental impact. In addition, the use of pesticides has become an obstacle to the fresh agricultural products exported to many countries that restrict pesticides residues. The aim of this study was to propose alternatives based on the use of microbiological products for the control of C. capitata. Main body The pathogenicity of the Turkish isolate Beauveria bassiana BMAUM M6-4 was evaluated against immature stages and adult of C. capitata under laboratory conditions at a concentration of 107 conidia/ml via different routes of exposure. Conidial treatment was applied by spraying soil and filter paper against 3rd instar larvae and pupae. In contrast, the treatment was administered to adult males and females by contact and ingestion. The percentage of mortality was recorded on the 3rd, 7th, 12th and 15th day after inoculation. Obtained results showed that B. bassiana was very effective against the adult’s fly, where males were more susceptible than females in both treatments (contact and ingestion). This isolate was tested against 3rd instar larvae. It achieved an infection rate of 33.33 and 43.5% of the larvae treated on filter paper and in the soil, respectively. Application of conidial preparations to soil reduced insect emergence and CAN represent a promising strategy for fruit fly integrated management (57.33 and 56.66% emergence from the treatments in soil and filter paper). Conclusion Entomopathogenic fungi are promising as a biocontrol agent that can be used under different modes of ground application against larvae, prepupae and pupae, and/or as a bait spraying or contact against adults.

Effectiveness of Entomopathogenic Nematodes Against Ceratitis capitata (Diptera: Tephritidae) Pupae and Nematode Compatibility with Chemical Insecticides

2021 ◽  
Author(s):  
Maguintontz Cedney Jean-Baptiste ◽  
Andressa Lima de Brida ◽  
Daniel Bernardi ◽  
Sérgio da Costa Dias ◽  
Juliano de Bastos Pazini ◽  
...  
Keyword(s):  
Biological Control ◽  
Entomopathogenic Nematodes ◽  
Ceratitis Capitata ◽  
Integrated Management ◽  
Fruit Fly ◽  
Steinernema Carpocapsae ◽  
Mediterranean Fruit Fly ◽  
Fruit Crops ◽  
Class 1 ◽  
Pupal Mortality

Abstract The Mediterranean fruit fly Ceratitis capitata (Wiedemann, 1824) (Diptera: Tephritidae) is among the main pests of fruit crops worldwide. Biological control using entomopathogenic nematodes (EPNs) may be an alternative to suppress populations of this pest. Thus, the aim of this study was to evaluate the pathogenicity and virulence of six EPN isolates (Heterorhabditis bacteriophora HB, H. amazonensis IBCB-n24, Steinernema carpocapsae IBCB-n02, S. rarum PAM-25, S. glaseri IBCB-n47, and S. brazilense IBCB-n06) against C. capitata pupae. The compatibility of EPNs with different chemical insecticides that are registered for management of C. capitata was also assessed. Isolates of H. bacteriophora HB and S. brazilense IBCB-n06 at a concentration of 1,000 infective juveniles (IJ)/ml proved to be most pathogenic to C. capitata (70 and 80% mortality, respectively). In contrast, the isolates H. amazonensis IBCB-n24, Steinernema carpocapsae IBCB-n02, S. rarum PAM-25, S. glaseri IBCB-n47 provided pupal mortality of less than 60%. Bioassays to determine lethal concentrations indicated that concentrations of 600 IJ/ml (H. bacteriophora HB) and 1,000 IJ/ml (S. brazilense IBCB-n06) showed the highest virulence against C. capitata pupae. In contrast, the highest numbers of IJs emerged at concentrations of 1,200 and 200 IJ/ml. In compatibility bioassays, malathion, spinetoram, phosmet, acetamiprid, and novaluron were considered compatible with and harmless (Class 1) to H. bacteriophora HB and S. brazilense IBCB-n06, according to IOBC/WPRS. This information is important for implementing integrated management programs for C. capitata, using biological control with EPNs, whether alone or in combination with chemical insecticides.

scholarly journals Genetic analysis of medfly populations in an area of sterile insect technique applications

2021 ◽  
Author(s):  
Rubén Sancho ◽  
Ana Guillem-Amat ◽  
Elena López-Errasquín ◽  
Lucas Sánchez ◽  
Félix Ortego ◽  
...  
Keyword(s):  
Genetic Markers ◽  
Sterile Insect Technique ◽  
Ceratitis Capitata ◽  
Natural Populations ◽  
Low Frequency ◽  
Fruit Fly ◽  
Wild Populations ◽  
Genetic Sexing ◽  
Environmental Implications ◽  
Genetic Sexing Strains

AbstractThe sterile insect technique (SIT) is widely used in integrated pest management programs for the control of the Mediterranean fruit fly (medfly), Ceratitis capitata. The genetic interactions between the released individuals from the genetic sexing strains (GSS), used for SIT applications worldwide, and wild individuals have not been studied. Under the hypothesis that a number of Vienna GSS individuals released to the field might not be completely sterile and may produce viable offspring, we have analyzed medfly Spanish field populations to evaluate the presence of Vienna strain genetic markers. To this goal, we have used contrasted nuclear and mitochondrial genetic markers, and two novel sets of nuclear polymorphisms with the potential to be markers to discriminate between Vienna and wild individuals. Nuclear Vienna markers located on the 5th chromosome of Vienna males have been found in 2.2% (19 from 875) of the Spanish wild medfly females captured at the area where SIT is applied. In addition, a female-inherited mitochondrial Vienna marker has been found in two from the 19 females showing nuclear Vienna markers. The detection of several of these markers in single individuals represents evidence of the introgression of Vienna strain into natural populations. However, alternative explanations as their presence at low frequency in wild populations in the studied areas cannot be fully discarded. The undesired release of non-fully sterile irradiated GSS individuals into the field and their interactions with wild flies, and the potential environmental implications should be taken into account in the application of the SIT.

scholarly journals Bioinvasions of the Medfly Ceratitis capitata: Source Estimation Using DNA Sequences at Multiple Intron Loci

Genetics ◽  
1999 ◽  
Vol 153 (1) ◽  
pp. 351-360 ◽  
Author(s):  
Neil Davies ◽  
Francis X Villablanca ◽  
George K Roderick
Keyword(s):  
Population Structure ◽  
Latin America ◽  
Dna Sequences ◽  
Ceratitis Capitata ◽  
Fruit Fly ◽  
Ecological Processes ◽  
The Status ◽  
Considerable Controversy ◽  
Source Populations ◽  
Traditional Population

Abstract The Mediterranean fruit fly, Ceratitis capitata, is a devastating agricultural pest that threatens to become established in vulnerable areas such as California and Florida. Considerable controversy surrounds the status of Californian medfly infestations: Do they represent repeated introductions or the persistence of a resident population? Attempts to resolve this question using traditional population genetic markers and statistical methods are problematic because the most likely source populations in Latin America were themselves only recently colonized and are genetically very similar. Here, significant population structure among several New World medfly populations is demonstrated through the analysis of DNA sequence variation at four intron loci. Surprisingly, in these newly founded populations, estimates of population structure increase when measures of subdivision take into account the relatedness of alleles as well as their frequency. A nonequilibrium, likelihood-based statistical test that utilizes multilocus genotypes suggests that the sole medfly captured in California during 1996 was introduced from Latin America and was less likely to be a remnant of an ancestral Californian population. Many bioinvasions are hierarchical in nature, consisting of several sequential or overlapping invasion events, the totality of which can be termed a metainvasion. Phylogenetic data from multilocus DNA sequences will be vital to understanding the evolutionary and ecological processes that underlie metainvasions and to resolving their constituent levels.

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