scholarly journals HONEY SUPPLEMENTATION IN LACTATE RINGER-EGG YOLK EXTENDER ON QUALITY OF PELUNG CHICKEN SPERMATOZOA POST-CHILLING

2021 ◽  
Vol 15 (1) ◽  
Author(s):  
Nu'man - Hidayat ◽  
Ismoyowati - Ismoyowati ◽  
Chomsiatun Nurul Hidayah ◽  
Aras Prasetiyo Nugroho
Keyword(s):  
Vitamin E ◽  
Sodium Dodecyl Sulfate ◽  
Sperm Motility ◽  
Sodium Dodecyl ◽  
Egg Yolk ◽  
Lactate Ringer ◽  
Treatment Groups ◽  
Dodecyl Sulfate ◽  
Abdominal Massage

The purpose of this research was to determine the influence of honey supplementation in lactate ringer-egg yolk extender with 0.025% sodium dodecyl sulfate and 2% vitamin E addition (LREYSE) on the quality of Pelung chicken spermatozoa preserved at 5° C for 72 hours.Semen was collected from three Pelung chickens once per day over a course of three days using the dorsal-abdominal massage method. Semen was divided into 5 treatment groups of honey supplementation that are 0% as control (LREYSEH0), 1% (LREYSEH1), 2% (LREYSEH2), 3% (LREYSEH3), and 4% (LREYSEH4). This liquid semen was observed for sperm motility and viability every 12 hours. Complete random designrepeated measurement with 4 replications was used in this study. The results showed the motility and viability of spermatozoa in LREYSEextender with 2% honey supplementation (61.25±1.25% and 71.50±0.74%) was significantly higher (P0.05) than other treatments that are 0% (51.25±1.25% and 61.88±1.36%), 1% (52.50±1.44% and 63.25±1.38%), 3% (51.25±1.25% and 61.63±1.48%), and 4% (50.00±2.04% and 60.63±2.29%) of honey supplementation in extender at 36 hours of storage until the end of the observation at 72 hours of incubation. According to the results of this study, it can be concluded that the 2% honey supplementation in extender is the best treatment to maintain sperm motility and viability for 72 hours of storage.

scholarly journals Reduction Spermatozoa Motility and Viability on Various Local Chickens During Storage at 5℃

2021 ◽  
Vol 22 (3) ◽  
pp. 158-162
Author(s):  
Nu'man Hidayat ◽  
Chomsiatun Nurul Hidayah ◽  
Aras Prasetiyo Nugroho
Keyword(s):  
Vitamin E ◽  
Sodium Dodecyl Sulfate ◽  
Sperm Motility ◽  
Sodium Dodecyl ◽  
Egg Yolk ◽  
Sperm Viability ◽  
Spermatozoa Motility ◽  
Abdominal Massage ◽  
Range Test ◽  
Local Chickens

The research compared the changes motility and viability of sperm from various local chickens during storage at 5℃ for 72 hours. Semen was collected every three days using the dorsal-abdominal massage from twelve chickens consisting of Kedu, Sentul and Pelung chicken. semen was diluted in extender contains 90% lactate ringer and 10% egg yolk extender with 0.025% sodium dodecyl sulfate and 2% vitamin E (LREYSE). Sperm motility and viability was observed every 12 hours and the measurements were made up to 72 hours of storage. Complete random design repeated measurement with 4 replications was used in this study. One-way analysis of variance was used to analyze the data and followed by Duncan’s Multiple Range Test. The results showed sperm motility declined and sperm viability reduced during storage for all breeds. The significant declined of motility between breeds were only observed at 60 and 72 hours and the reduced viability between breeds was observed at 72 hours of storage. The declined sperm motility for Kedu chicken (31.59±3.26% and 75.36±1.25%) and Pelung chicken (36.11±4.05% and 75.83±5.34%) were significantly lower (p<0.05) than Sentul Chicken (50.39±2.60% and 95.00±5.00%) at 60 and 72 hours of storage respectively, while the decline sperm viability of Kedu chicken (57.59±3.64%) and Pelung chicken (54.39±5.73%) was significantly lower (p<0.05) than Sentul Chicken (90.30±9.70%) after 72 hours storage. It can be concluded that the reduction sperm motility and viability of Kedu and Pelung chicken is lower than Sentul chicken which are stored at 5℃ for 72 hours.

scholarly journals PENENTUAN KONSENTRASI SODIUM DODECYL SULFATE DALAM PENGENCER RINGER LAKTAT-KUNING TELUR UNTUK PRESERVASI SEMEN AYAM PELUNG (Determination of Sodium Dodecyl Sulfate Concentration in Ringer Lactate-Egg Yolk Extender for Pelung Rooster Semen Preservation)

2016 ◽  
Vol 10 (2) ◽  
pp. 170-174
Author(s):  
Nu’man Hidayat ◽  
Cece Sumantri ◽  
Rudi Afnan
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Sperm Motility ◽  
Sodium Dodecyl ◽  
Egg Yolk ◽  
Ringer Lactate ◽  
Spermatozoa Motility ◽  
Dodecyl Sulfate ◽  
Semen Preservation ◽  
Better Than

The objective of this experiment was to determine the addition of sodium dodecyl sulfate (SDS) to Ringer Lactate-Egg Yolk (RL-EY) extender on pelung chicken semen preservation. Semen was collected three times a week from three pelung chickens. Collected semen was evaluated macroscopically and microscopically. Only ejaculates of at least 70% sperm motility was then divided into three equal microtubes. Each of them diluted with RL-EY with ratio 90%:10%, then added with 0.00, 0.025, and 0.05% SDS respectively. The liquid semen then stored at 5 °C for 72 hours. Sperm motility and viability were observed every 12 hours. The addition of 0.025% SDS showed higher spermatozoa motility and viability (72.08±1.44% and 80.82±1.30%) which were significantly higher (P<0.05) than 0.00 and 0.05% SDS addition at 24 hours of storage. There was no differences on the spermatozoa motility and viability between 0.00 and 0.05% SDS addition. The decrease of spermatozoa motility and viability was observed in 0.025% SDS addition (4.17±0.56% and 4.65±0.59%, respectively) that significantly lower compared to 0% and 0.05% SDS addition at 24 hours of storage. In conclusion, 0.025% SDS addition in a Ringer Lactate -Egg Yolk extender maintained pelung spermatozoa motility and viability better than 0.00 and 5% SDS addition.

scholarly journals Chitosan/Sodium Dodecyl Sulfate Complexes for Microencapsulation of Vitamin E and Its Release Profile—Understanding the Effect of Anionic Surfactant

2021 ◽  
Vol 15 (1) ◽  
pp. 54
Author(s):  
Jelena Milinković Budinčić ◽  
Lidija Petrović ◽  
Ljiljana Đekić ◽  
Milijana Aleksić ◽  
Jadranka Fraj ◽  
...  
Keyword(s):  
Vitamin E ◽  
Sodium Dodecyl Sulfate ◽  
Anionic Surfactant ◽  
Sodium Dodecyl ◽  
Pharmaceutical Products ◽  
Release Mechanism ◽  
Bioactive Substances ◽  
Water Emulsion ◽  
Dodecyl Sulfate

Microencapsulation of bioactive substances is a common strategy for their protection and release rate control. The use of chitosan (Ch) is particularly promising due to its abundance, biocompatibility, and interaction with anionic surfactants to form complexes of different characteristics with relevance for use in microcapsule wall design. In this study, Ch/sodium dodecyl sulfate (SDS) microcapsules, without and with cross-linking agent (formaldehyde (FA) or glutaraldehyde (GA)), were obtained by the spray drying of vitamin E loaded oil-in-water emulsion. All of the microcapsules had good stability during the drying process. Depending on the composition, their product yield, moisture content, and encapsulation efficiency varied between 11–34%, 1.14–1.62%, and 94–126%, respectively. SEM and FTIR analysis results indicate that SDS as well as cross-linkers significantly affected the microcapsule wall properties. The profiles of in vitro vitamin E release from the investigated microcapsules fit with the Korsmeyer-Peppas model (r2 > 0.9). The chemical structure of the anionic surfactant was found to have a significant effect on the vitamin E release mechanism. Ch/SDS coacervates may build a microcapsule wall without toxic crosslinkers. This enabled the combined diffusion/swelling based release mechanism of the encapsulated lipophilic substance, which can be considered favorable for utilization in food and pharmaceutical products.

Use of Sodium Dodecyl Sulfate Pretreatment and 2-stage Curing for Improved Quality of Salted Duck Eggs

2014 ◽  
Vol 79 (3) ◽  
pp. E354-E361 ◽  
Author(s):  
Zixuan Lian ◽  
Longshan Qiao ◽  
Guanghong Zhu ◽  
Yun Deng ◽  
Bingjun Qian ◽  
...  
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Sodium Dodecyl ◽  
Dodecyl Sulfate ◽  
Salted Duck Eggs

scholarly journals Adding of L-Arginin Amino Acidin Skim Milk Diluent to Maintain Quality of Buck Sperm in Cold Temperature

2017 ◽  
Vol 3 (6) ◽  
pp. 189
Author(s):  
Tri Wahyu Suprayogy ◽  
Suherni Susilowati ◽  
Tatik Hernawati
Keyword(s):  
Amino Acid ◽  
Sperm Motility ◽  
Seminal Plasma ◽  
Membrane Integrity ◽  
Skim Milk ◽  
Egg Yolk ◽  
Cold Temperature ◽  
Treatment Groups

Nowdays, the storage of buck semen in cold temperature have not satisfied yet  because in  buck’s seminal plasma contains phospholipase enzyme which can coagulated egg yolk in diluents.The specific aim of this study was to investigate the benefits of  L-Arginin amino acid in skim diluents to quality  buck’s spermatozoa on cold temperature. This researchutilized four treatment groups, namely Controlled group (P0): skim milk diluent without L-Arginin + buck’s semen; P1: skim milk diluents + L-Arginin 0,002M/ml + buck” semen; P2: skim milk diluents + L-Arginin 0,004 M/ml + buck’s semen and P3: skim milk diluents + L-Arginin 0,006 M/ml + buck’s semen. Then the samples stored in cold temperature (5oC). The result showed that sperm motility, viability and membrane integrity were significantly different (p<0,05) among the treatments.The conclusion of this study is adding of L-Arginin Amino Acid in skim milk diluents maintain motility, viability and membrane integrity  buck’s sperm. Keywords: L-Arginin, buck, cold temperature, motility, viability and membrane integrity

scholarly journals The Effects of Additives, Particles Load and Current Density on Codeposition of SiC Particles in NiP Nanocomposite Coatings

Coatings ◽  
2019 ◽  
Vol 9 (9) ◽  
pp. 554 ◽  
Author(s):  
Donya Ahmadkhaniha ◽  
Caterina Zanella
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Current Density ◽  
Composite Coatings ◽  
Sodium Dodecyl ◽  
Low Carbon Steel ◽  
Low Carbon ◽  
Sic Particles ◽  
P Content ◽  
Dodecyl Sulfate

In this study, electrodeposition of NiP composite coatings with the addition of SiC 100 nm was carried out on low carbon steel studying the effect of additives (sodium dodecyl sulfate, saccharin), particles load (10 or 20 g/L) and current density (1, 2 and 4 A/dm2). As a benchmark, coatings from an additive-free bath were also deposited, despite additives being essential for a good quality of the coatings. The coating’s morphology and composition were evaluated by scanning electron microscope (SEM) equipped with energy dispersive spectroscopy (EDS). It was shown that by addition of sodium dodecyl sulfate (SDS), pure NiP coating with a higher P content was achieved, and their morphology changed to nodular. SDS also reduced the codeposited fraction of SiC particles, while saccharin increased it. SiC loading and current density had less impact respect to the additives on codeposition of SiC particles. Finally, the microhardness of NiP coatings did not increase linearly by codeposition of SiC particles.

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