scholarly journals Phenotypes of Streptococcus mutans isolates in sound and cavitated tooth surfaces and its relationship to early childhood caries in China

2020 ◽  
Author(s):  
Shanshan Liu ◽  
Huihui Li ◽  
Zhenfei Guo ◽  
Yu Sun ◽  
Qingwei Zheng ◽  
...  
Keyword(s):  
Early Childhood ◽  
Streptococcus Mutans ◽  
Early Childhood Caries ◽  
Tooth Surfaces ◽  
Childhood Caries

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scholarly journals Phenotypes of Streptococcus mutans isolates in sound and cavitated tooth surfaces and its relationship to early childhood caries in China

2019 ◽  
Author(s):  
Shanshan Liu ◽  
Huihui Li ◽  
Zhenfei Guo ◽  
Yu Sun ◽  
Qingwei Zheng ◽  
...  
Keyword(s):  
Early Childhood ◽  
Streptococcus Mutans ◽  
Biofilm Formation ◽  
Early Childhood Caries ◽  
Single Subject ◽  
Expression Levels ◽  
Tooth Surfaces ◽  
Lower Expression ◽  
Low Expression ◽  
Childhood Caries

Abstract Background To compare the phenotypes of Streptococcus mutans (S. mutans ) strains isolated from sites with different caries susceptibility of a single subject. Methods This study was carried out in children with early-childhood caries (ECC) and caries-free (CF). The ECC subjects presented two sites: a cavitated lesion (ECC-C) and a sound surface (ECC-F). The CF subjects presented one sound surface. The following phenotypes were evaluated among these three sites: growth pattern, biofilm, exopolysaccharide (EPS), pH drop, and the expression levels of genes (gtfB, gtfC, spaP, srtA, relA, ffh, srn225147, and srn821798). Results All of these phenotypes were detected similarly between ECC-C and ECC-F groups. However, the capacity of biofilm formation, EPS, pH drop and the expression levels of genes (gtfB and spaP) of S. mutans in the CF group were lower compared to those of the ECC group. There was a relatively higher expression of srn821798 compared to that of srn225147 in clinical isolates, and isolates with low expression of srn821798 had lower expression levels of predicted targets (gtfB, gtfC, and spaP). Conclusions These data indicate S. mutans isolated from sites with different caries susceptibility of a single subject showed similar characteristics, while the cariogenic potential of the S. mutans isolated from ECC individuals was relatively higher than that of CF subjects.

Effect of Sustained Interventions from Infancy to Toddlerhood in Children with Cleft Lip and Palate for Preventing Early Childhood Caries

2021 ◽  
pp. 1-9
Author(s):  
Sivasubramanian Abirami ◽  
Noopur Panchanadikar ◽  
Murugan Satta Muthu ◽  
Suganya Balasubramanian ◽  
Jyotsna Murthy ◽  
...  
Keyword(s):  
Early Childhood ◽  
Early Childhood Caries ◽  
Cleft Lip ◽  
Cleft Lip And Palate ◽  
Intervention Group ◽  
Primary Caregivers ◽  
Assessment System ◽  
Incidence Density ◽  
Tooth Surfaces ◽  
Childhood Caries

The study aimed to evaluate the effectiveness of sustained interventions in children with cleft lip and palate (CLP) for preventing early childhood caries (ECC). This prospective, nonrandomized interventional cohort study was conducted in infants aged 0–12 months with congenital CLP. Interventions were given to parents/primary caregivers in the form of combined oral health-care measures (sterile wet gauze piece, finger brush, toothbrush, and toothpaste) by a motivational interviewing approach. Education of primary caregivers on oral hygiene was provided by audiovisual aids and demonstration. Reinforcement of the prescribed regimen was done through daily short message services in caregivers’ preferred language and bimonthly telephone calls. Participants were followed up for 9–32 months from the time of recruitment, with a mean period of 18.3 ± 5.1 months. Rates of dental caries were represented as prevalence rates, incidence density, and transitional probability. The distribution of the International Caries Detection and Assessment System (ICDAS) scores on different tooth surfaces affected in the intervention group was compared descriptively with that of the age- and sex-matched historical control groups. On analysis of surface-wise distribution of the ICDAS scores in the intervention group (<i>n</i> = 1,919), 1.2% (<i>n</i> = 24) had noncavitated lesions (ICDAS codes 1 and 2), 0.88% (<i>n</i> = 17) had cavitated lesions (ICDAS codes 3–6), and 0.26% (<i>n</i> = 5) had both cavitated and noncavitated lesions (ICDAS codes 1–6). The incidence density of caries-affected children observed at the first and last follow-ups was 1.2 persons/100 person-months and 1.3 persons/100 person-months of observation, respectively. The incidence density of new caries-affected tooth surfaces at the first and last follow-ups was 0.163 surfaces/100 surface-months and 0.062 surfaces/100 surface-months, respectively. Maxillary first molars had the maximum transition from sound to the cavitated lesion (11.5%), followed by maxillary incisors from sound to noncavitated (7.5%) at the last follow-up. Based on the newly developed assessment criteria in our study, sustained interventions proved to be significantly effective in preventing ECC in children with CLP.

scholarly journals Relationship between Candida albicans and Streptococcus mutans in early childhood caries, evaluated by quantitative PCR

F1000Research ◽  
2018 ◽  
Vol 7 ◽  
pp. 1645 ◽  
Author(s):  
Endang W. Bachtiar ◽  
Boy M. Bachtiar
Keyword(s):  
Early Childhood ◽  
Candida Albicans ◽  
Streptococcus Mutans ◽  
Dental Plaque ◽  
Early Childhood Caries ◽  
Positive Correlation ◽  
Unstimulated Saliva ◽  
Student’S T ◽  
Total Bacteria ◽  
Childhood Caries

Background:The aim of this study was to analyze the synergistic relationship betweenCandida albicansandStreptococcus mutansin children with early childhood caries (ECC) experience.Methods:Dental plaque and unstimulated saliva samples were taken from 30 subjects aged 3-5 years old, half with (n=15, dmft > 4) and half without (n=15) ECC. The abundance ofC. albicansandS. mutansand relative to total bacteria load were quantify by real-time PCR (qPCR). This method was also employed to investigate the mRNA expression of glycosyltransferase (gtfB) gene in dental plaque. Student’s t-test and Pearson’s correlation were used to perform statistical analysis.Results:Within the ECC group, the quantity of both microorganisms were higher in the saliva than in dental plaque. The ratio ofC. albicansto total bacteria was higher in saliva than in plaque samples (p < 0.05). We observed the opposite forS. mutans(p < 0.05). The different value ofC. albicansandS. mutansin saliva was positively correlated, and negatively correlated in dental plaque. Transcription level ofS. mutans gtfBshowed a positive correlation withC. albicansconcentration in dental plaque. Conclusion:C. albicanshas a positive correlation with cariogenic traits ofS. mutansin ECC-related biofilm of young children.

Streptococcus Mutans Strains in Mother-Child Pairs of Children with Early Childhood Caries

2019 ◽  
Vol 43 (4) ◽  
pp. 252-256 ◽  
Author(s):  
Priya Subramaniam ◽  
Revathy Suresh
Keyword(s):  
Early Childhood ◽  
Dental Caries ◽  
Streptococcus Mutans ◽  
Early Childhood Caries ◽  
Mutans Streptococci ◽  
Similar Distribution ◽  
Healthy Children ◽  
Only Children ◽  
Polymerase Chain ◽  
Childhood Caries

Objective: Dental caries is both an infectious and transmissible disease. Maternal transfer of Mutans Streptococci occurs at an early age and is important in the initiation of dental caries in children. The aim of this study was to identify certain strains of Streptococcus mutans in mother-child pairs, of children with early childhood caries. Study design: Sixty mother-child pairs of healthy children aged 18–36 months were selected. Mothers with high levels of Streptococcus mutans in their saliva and only children with ECC were included. Dental plaque samples were collected from mother-child pairs. The plaque samples were stored, transferred to the laboratory and analyzed for Streptococcus mutans strains c, f, e and k, present in mother-child pairs using Real time Polymerase Chain Reaction (PCR) technique. Data obtained was subjected to statistical analysis for level of similarity in Streptococcus mutans strains present in mother-child pairs. Results: A similar distribution of Streptococcus mutans strains c, f and k was identified in 28 mother-child pairs. Streptococcus mutans strain e was seen in 18 pairs. Conclusion: Less than 50% of mother-child pairs showed similarity in distribution of Streptococcus mutans strains.

scholarly journals Association of Feeding Methods and Streptococcus mutans Count with Early Childhood Caries: A Cross-sectional Study

2017 ◽  
Vol 10 (2) ◽  
pp. 119-125 ◽  
Author(s):  
Manjunath P Puranik ◽  
Deepa Bullappa ◽  
KR Sowmya ◽  
T Nagarathnamma
Keyword(s):  
Early Childhood ◽  
Streptococcus Mutans ◽  
Early Childhood Caries ◽  
Cross Sectional Study ◽  
Caries Experience ◽  
Sectional Study ◽  
Cross Sectional ◽  
Mothers And Children ◽  
Feeding Methods ◽  
Childhood Caries

ABSTRACT Introduction Early childhood caries (ECC) is a virulent form of dental caries that can destroy the primary dentition of toddlers and preschool children. The aim was to determine the relationship of feeding methods and oral Streptococcus mutans count in 3- to 5-year-old children with ECC. Materials and methods A cross-sectional study was conducted in children aged 3 to 5 years. Participating mothers were interviewed regarding child's demographic profile, educational level and socioeconomic status of parents, past medical and dental history of the mother and child, child's feeding habits, and dietary habits and oral hygiene practices of mother and child. Clinical examination for dental caries was done using the World Health Organization criteria (1997). Salivary samples of mother–child pair were collected to determine the pH, flow rate, and S. mutans count. Statistical tests, such as Student's t-test, analysis of variance, and Pearson's correlation were applied. Results Out of 150 mother–child pair, statistically significant difference in the caries experience was found between mothers and children with high and low S. mutans count. Moderate but statistically significant negative correlation was found between mean decayed, missing, and filled teeth of mothers and mean decayed, extracted and filled teeth (deft) of children with high S. mutans count. Regarding deft, there was no statistically significant difference between children who were exclusively breast fed (7.85 ± 2.94), exclusively bottle-fed (8.67 ± 3.98), and both breast and bottle-fed (7.77 ± 2.91). Conclusion The mean caries experience of mothers and children was 2.66 ± 2.01 and 7.82 ± 2.94 respectively, with decayed component being maximum. Moderate and significant correlation (r = 0.5) was found between S. mutans of mothers and children in saliva. Significant negative correlation was found between mothers and children with high S. mutans count (r = –0.0284; p = 0.046). How to cite this article Bullappa D, Puranik MP, Sowmya KR, Nagarathnamma T. Association of Feeding Methods and Streptococcus mutans Count with Early Childhood Caries: A Cross-sectional Study. Int J Clin Pediatr Dent 2017;10(2):119-125.

Streptococcus mutans isolated from children with severe-early childhood caries form higher levels of persisters

2020 ◽  
Vol 110 ◽  
pp. 104601 ◽  
Author(s):  
Aaron Bottner ◽  
Richard Y. He ◽  
Andrea Sarbu ◽  
S.M. Hashim Nainar ◽  
Delphine Dufour ◽  
...  
Keyword(s):  
Early Childhood ◽  
Streptococcus Mutans ◽  
Early Childhood Caries ◽  
Severe Early Childhood Caries ◽  
Childhood Caries

Longitudinal Evaluation of Salivary Iga-S in Children with Early Childhood Caries Before and After Restorative Treatment

2019 ◽  
Vol 43 (4) ◽  
pp. 239-243 ◽  
Author(s):  
Aline dos Santos Letieri ◽  
Liana Bastos Freitas-Fernandes ◽  
Ana Paula Canedo Valente ◽  
Tatiana Kelly da Silva Fidalgo ◽  
Ivete Pomarico Ribeiro de Souza
Keyword(s):  
Early Childhood ◽  
Streptococcus Mutans ◽  
Flow Rate ◽  
Total Protein ◽  
Early Childhood Caries ◽  
Dental Treatment ◽  
Restorative Treatment ◽  
Lactobacillus Spp ◽  
Childhood Caries

Background: Our aim was to compare salivary levels of secretory immunoglobulin A (s-IgA) in children with early childhood caries (ECCG) and those who are caries-free (CFG) and verify these levels in a follow-up period after restorative treatment. Materials and methods: We selected 46 systemically healthy children in the complete primary dentition period, who were allocated into two groups: CFG (n = 23) and ECCG (dmf-s &gt; 0; n = 23). Unstimulated whole saliva was obtained at baseline from both groups and during the follow-up period (7 days, 1, 2 and 3 months) in the ECCG group. The s-IgA was measured using an ELISA assay, and total protein was assessed using the Bradford method. We also evaluated the flow rate (mL/min), Streptococcus mutans and Lactobacillus spp. counting using selective media plaques. The data were submitted to statistical analysis using the software SPSS 20.0 (SPSS Inc, IL, USA) with a confidence interval set at 95%. Results: Salivary s-IgA levels were higher in baseline of ECCG than in CFG (p&lt;0.05). No statistically significant differences were observed between s-IgA salivary levels at baseline and the evaluations after dental treatment in ECCG (p&gt;0.05). However, we observed two different changes in s-IgA levels among participants: one group presented s-IgA reduction, and the other group demonstrated its maintenance. It was shown that patients from the ECCG group who presented a reduction in s-IgA levels during follow-up also showed a decrease in Streptococcus mutans and Lactobacillus spp. count (p&lt;0.05), in contrast to patients who did not present this reduction. The flow rate and total protein were similar between groups (p&gt;0.05). Conclusions: The present data support the idea that children with early childhood caries present higher levels of s-IgA in saliva than caries-free children. The restorative dental treatment does not have a significant influence on salivary levels of this immunoglobulin during the follow-up period.

scholarly journals Early childhood feeding practices and dental caries among Australian preschoolers

2020 ◽  
Vol 111 (4) ◽  
pp. 821-828 ◽  
Author(s):  
Gemma Devenish ◽  
Aqif Mukhtar ◽  
Andrea Begley ◽  
A John Spencer ◽  
W Murray Thomson ◽  
...  
Keyword(s):  
Early Childhood ◽  
Early Childhood Caries ◽  
Feeding Practices ◽  
Sociodemographic Factors ◽  
Breastfeeding Duration ◽  
Dietary Practices ◽  
Free Sugars ◽  
Breastfeeding Practices ◽  
Tooth Surfaces ◽  
Childhood Caries

ABSTRACT Background Recent reviews have proposed a causal relationship between prolonged breastfeeding and early childhood caries (ECC), but the evidence to date is inconsistent, with few cohort studies and limited investigation of key confounders. Objective This study aimed to investigate the relationship between dietary practices and early childhood caries in a birth cohort of Australian preschoolers. Methods Participants underwent a standardized dental examination at 2–3 y of age to determine the prevalence of ECC (based on the presence of decayed, missing, or filled tooth surfaces). Breastfeeding practices were reported at 3, 6, 12, and 24 mo of age. Intakes of free sugars were assessed at 1 and 2 y of age. Multivariable regression models generated prevalence ratios (PR) for the association between ECC and breastfeeding duration, and between ECC and sleep feeding practices at 1 y, controlling for sociodemographic factors and free sugars intake. Results There was no independent association between breastfeeding beyond 1 y of age and ECC (PR 1.42, 95% CI: 0.85, 2.38), or between breastfeeding to sleep and ECC (PR 1.12, 95% CI: 0.67, 1.88), although the direction of effect was suggestive of an association. The only factors independently associated with ECC were high free sugars intakes (PR 1.97, 95% CI: 1.13, 3.44), and greater socioeconomic disadvantage (PR 2.15, 95% CI: 1.08, 4.28). Most participants who were breastfed at 1 y of age had ceased by 18 mo or 2 y. Conclusions Breastfeeding practices were not associated with ECC. Given the wide-ranging benefits of breastfeeding, and the low prevalence of sustained breastfeeding in this study and Australia in general, recommendations to limit breastfeeding are unwarranted, and breastfeeding should be promoted in line with global and national recommendations. To reduce the prevalence of early childhood caries, improved efforts are needed to limit foods high in free sugars.

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