A new understanding of the H. pylori eradication mechanism

2019 ◽  
Author(s):  
Shanshan Su ◽  
Guo-qi Zheng ◽  
Ying-ying Liu ◽  
Yu-fei Liang ◽  
Hui Song ◽  
...  
Keyword(s):  
Gastric Juice ◽  
Intervention Group ◽  
Control Group ◽  
Rapid Urease Test ◽  
Mucus Layer ◽  
Culture Methods ◽  
Detection Rates ◽  
Urease Test ◽  
Gastric Lumen ◽  
H Pylori

Abstract Background: Helicobacter pylori (H. pylori) cannot usually be detected in the gastric juice and it is thought that H. pylori may be implanted under the mucus layer for long term. The mechanisms of action of proton pump inhibitor (PPI), antibiotics, and bismuth for H. pylori eradication are not entirely clear. Our study aimed to determine the role of PPI on the movement of H. pylori across the mucus layer to the gastric lumen and the mechanism of PPI, antibiotics, and bismuth on H. pylori eradication. Methods: Patients with H. pylori infection were intravenous injected with PPI (intervention group, n=31) or without PPI (control group, n=37). The presence of H. pylori in the gastric juice was evaluated by the rapid urease test (RUT), polymerase chain reaction (PCR), and culture methods. Results: The H. pylori positive detection rates were all significantly higher among patients in the intervention group than among patients in the control group by the RUT (P < 0.0001), PCR (P < 0.0001), and culturing (P = 0.0386). Conclusion: H. pylori can penetrate across the mucus layer to the gastric lumen following PPI intervention. The direct antimicrobial activity of PPI might because of diminished numbers of H. pylori due to probiotics in the gastric lumen. Antibiotics and bismuth might play a local sterilization role in the gastric lumen when H. pylori penetrate across the mucus layer.

scholarly journals Effect of proton pump inhibitor on the movement of Helicobacter pylori across the mucus layer to the gastric lumen

2019 ◽  
Author(s):  
shanshan Su(Former Corresponding Author) ◽  
Guo-qi Zheng(New Corresponding Author) ◽  
Ying-ying Liu ◽  
Yu-fei Liang ◽  
Hui Song ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Proton Pump Inhibitor ◽  
Gastric Juice ◽  
Proton Pump ◽  
Intervention Group ◽  
Control Group ◽  
Mucus Layer ◽  
Urease Test ◽  
Gastric Lumen ◽  
H Pylori

Abstract Background: Helicobacter pylori ( H. pylori ) cannot usually be detected in the gastric juice and it is thought that H. pylori may reside under the mucus layer for long term. The mechanisms of action of proton pump inhibitor (PPI)for H. pylori eradication are not entirely clear. Our study aimed to determine the role of PPI on the movement of H. pylori across the mucus layer to the gastric lumen and the mechanism of PPI on H. pylori eradication. Methods: Patients with H. pylori infection were intravenous injected with PPI (intervention group, n=31) or without PPI (control group, n=37). The presence of H. pylori in the gastric juice was evaluated by the rapid urease test (RUT), polymerase chain reaction (PCR), and culture methods. Results: The H. pylori positive detection rates were all significantly higher among patients in the intervention group than among patients in the control group by the RUT ( P < 0.0001), PCR ( P < 0.0001), and culturing ( P = 0.0386). Conclusion: H. pylori can penetrate across the mucus layer to the gastric lumen following PPI intervention, and thus it might represent a novel target in the eradication of H. pylori .

scholarly journals Comparing Multiplex PCR and Rapid Urease Test in the Detection ofH. pyloriin Patients on Proton Pump Inhibitors

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Thomas Chen ◽  
Xiangwen Meng ◽  
H. Zhang ◽  
Rebecca W. Tsang ◽  
Tat-Kin Tsang
Keyword(s):  
Multiplex Pcr ◽  
Diagnostic Value ◽  
Control Group ◽  
Rapid Urease Test ◽  
Negative Results ◽  
Multiplex Pcr Assay ◽  
Time Period ◽  
Urease Test ◽  
H Pylori ◽  
The One

Background. This study was conducted to assess the diagnostic value of a multiplex PCR assay to detectH. pyloriinfection and to further evaluate the negative results from the CLOtest on patients with and without PPI treatment. Methods. This study is a retrospective cohort that included 457 patients with symptoms of dyspepsia, who underwent upper endoscopy at Evanston and Glenbrook Northshore Hospital from June 2003 to October 2007. A total of 556 samples were reported with some patients having more than one test over the time period. The CLOtest was performed first on the gastric specimen and from that specimen, the DNA was isolated and the one-step multiplex PCR was performed.Results. By M-PCR testing, H. pyloriwas detected in 143 (52.2%) of 274 cases in the control group and 130 (46.1%) of 282 cases in patients on PPI treatment (P=0.1746). The CLOtest detected the presence ofH. pyloriin 4 (1.4%) of 282 cases from the same group receiving PPI treatment and 29 (10.6%) of 274 cases from the group not taking a PPI (P≤0.0001).Conclusion. Our PCR is sensitive enough to detect the presence ofH. pyloridespite being on PPI treatment.

scholarly journals Helicobacter pylori culture as a key tool for diagnosis in Colombia

2019 ◽  
Vol 13 (08) ◽  
pp. 720-726
Author(s):  
Diana F Rojas-Rengifo ◽  
Belen Mendoza ◽  
Carlos Jaramillo ◽  
Paula A Rodríguez-Urrego ◽  
José F Vera-Chamorro ◽  
...  
Keyword(s):  
Predictive Value ◽  
Optimal Growth ◽  
Rapid Urease Test ◽  
Culture Methods ◽  
Essential Information ◽  
Urease Enzyme ◽  
Urease Test ◽  
Gastric Biopsies ◽  
H Pylori ◽  
Sensitivity Specificity

Introduction: The presence of H. pylori in the stomach is associated with gastric pathologies. However, its diagnosis through culture methods is challenging because of its complex nutritional requirements and microaerophilic conditions for optimal growth. The preferred method for rapid diagnosis of H. pylori is the Rapid Urease Test (RUT) from human biopsies, which relies on the high activity of the urease enzyme present in H. pylori. However, RUT cannot say much more information about H. pylori. This makes evident the need for bacterial culture to know essential information such as the strain type, the kind of infection present and the bacteria’s antibiotic susceptibility. Methodology: Gastric biopsies from 347 patients were used for H. pylori isolation. We correlated the culture results with the RUT and histological grading used at Hospital Universitario Fundación SantaFe de Bogotá (HU-FSFB), Colombia. The concordance between techniques was determined by the Cohen’s Kappa coefficient (K). The sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) were also calculated. Results: The culture standardization was successful, and it could be applied for diagnosis in the clinical practice. H. pylori was positive by culture in 88 (26.34%) patients. The concordance of RUT and culture was strong (K= 0.805), and between histology and culture was moderate (K= 0.763) as well as for the gold standard defined and culture (K= 0.80). Conclusions: We present evidence that RUT and histological methods will be better interpreted for diagnosis of H. pylori if combined with bacterial isolation in cholesterol enriched culture.

scholarly journals Relevance of examination and treatment of the most common gastrointestinal disorders in children in Lithuania during the last decade

Medicina ◽  
2007 ◽  
Vol 44 (1) ◽  
pp. 72 ◽  
Author(s):  
Liutauras Labanauskas ◽  
Rūta Kučinskienė ◽  
Vaidotas Urbonas ◽  
Rūta Rokaitė ◽  
Neringa Libikaitė
Keyword(s):  
Duodenal Ulcer ◽  
Atopic Dermatitis ◽  
Abdominal Pain ◽  
Skin Rash ◽  
Endoscopic Examination ◽  
Gastrointestinal Disorders ◽  
Control Group ◽  
Rapid Urease Test ◽  
Urease Test ◽  
H Pylori

In the last decade, scientific studies in the field of children’s gastroenterology performed in Lithuania explored different problems: pathology of Helicobacter pylori infection and food allergy. Our studies Helirevealed that children with atopic dermatitis had gastrointestinal complaints (abdominal pain, diarrhea, distension and unstable stool, which appeared with the exacerbation of skin rash) more often as compared to nonallergic children of the control group. Abdominal pain in children with atopic dermatitis with local rash was more frequent and lasted longer than in control group children, whereas children with extended rash had stools more frequently. Gastrointestinal disorders in children with atopic dermatitis statistically significantly did not depend on the extent of skin rash and severity of atopic dermatitis. In our scientific research on the importance of H. pylori infection on children’s gastrointestinal system, children with chronic dyspepsia were examined. Endoscopy, rapid urease test, biopsies from antrum and corpus of stomach and their histological examination as well as serologic tests were done. According to the results obtained, we recommend to examine children with chronic dyspepsia in a complex way: not only endoscopic examination, but H. pylori diagnostic tests should be performed as well. Serologic test is not suitable for screening H. pylori infection in children. Considering this, we recommend to use no fewer than two different methods to diagnose this infection. The highest frequency of H. pylori infection was found in children with duodenal ulcer; histological changes in their gastric pylorus and corpus mucosa were greatest. More than half of children with nonulcer dyspepsia were infected with H. pylori. After eradication of H. pylori infection, the prevalence of dyspepsia in children with duodenal ulcer decreased.

scholarly journals Association of TP53 gene codon 72 polymorphism with Helicobacter pylori-positive non-cardia gastric cancer in Vietnam

2019 ◽  
Vol 13 (11) ◽  
pp. 984-991
Author(s):  
Thi Minh Thi Ha ◽  
Thanh Nha Uyen Le ◽  
Viet Nhan Nguyen ◽  
Van Huy Tran
Keyword(s):  
Gastric Cancer ◽  
Control Group ◽  
Rapid Urease Test ◽  
Genotype Distribution ◽  
Codon 72 ◽  
Codon 72 Polymorphism ◽  
Urease Test ◽  
Significant Difference ◽  
Tp53 Codon 72 Polymorphism ◽  
H Pylori

Introduction: This research aimed to determine the association of the combination of H. pylori infection and TP53 codon 72 polymorphism with non-cardia gastric cancer (GC) in Vietnam. Methodology: A total of 164 patients with non-cardia GC and 164 patients with peptic ulcer disease or functional dyspepsia in controls matched by sex and age were enrolled. H. pylori infection was diagnosed by rapid urease test and polymerase chain reaction (PCR). The cagA gene-positivity and vacA sm subtypes were determined by multiplex PCR. Genotypes of TP53 codon 72 polymorphism were determined by PCR-restriction fragment length polymorphism. Results: The prevalence of H. pylori infection in GC and control group were 61.6% and 55.4%, respectively. The rates of cagA-positive strains in the two H. pylori-positive groups were 80.2% and 71.4%, respectively. There was no statistically significant difference in TP53 codon 72 genotype distribution between GC group (frequencies of Arg/Arg, Arg/Pro and Pro/Pro genotypes were 31.1%, 43.3% and 25.6%, respectively) and controls (29.3%, 52.4% and 18.3%, respectively), p = 0.172. The significant difference in genotype distribution was observed in recessive model (Pro/Pro vs Arg/Arg + Arg/Pro) when stratifying by H. pylori infection (OR = 2.02, 95% CI 1.03–3.96, p = 0.041) and by cagA-positivity (OR = 2.33, 95% CI 1.07–5.07, p = 0.032). Conclusions: This study suggests a synergistic interaction between H. pylori infection, especially cagA-positive H. pylori, and Pro/Pro genotype of TP53 codon 72 polymorphism might play a significant role in the pathogenesis of GC in the Vietnamese population.

scholarly journals Evidence of mother-child transmission of Helicobacter pylori infection

2004 ◽  
Vol 41 (4) ◽  
pp. 239-244 ◽  
Author(s):  
Mario Luis Escobar ◽  
Elisabete Kawakami
Keyword(s):  
Duodenal Ulcer ◽  
Helicobacter Pylori ◽  
Family Members ◽  
Control Group ◽  
Common Source ◽  
Rapid Urease Test ◽  
Source Of Infection ◽  
Group I ◽  
Urease Test ◽  
H Pylori

BACKGROUND: Low socioeconomical status is a major risk factor for natural acquisition of Helicobacter pylori (H. pylori) infection in developing countries. Its transmission route is unknown but studies suggest person-to-person transmission. AIM: To evaluate seropositivity of anti-H. pylori antibodies in family members of infected symptomatic index patients as compared to family members of symptomatic uninfected index patients. PATIENTS AND METHODS: One hundred and twelve family members of 38 patients who underwent endoscopy to exclude peptic disease were studied. Patients were deemed H. pylori infected or not infected when rapid urease test and histology were both positive or both negative. The family members underwent ELISA serology using the Cobas Core II Kit (Roche) and were classified into three groups: I - 29 family members of 10 H. pylori (+) duodenal ulcer index patients; II - 57 family members of 17 H. pylori (+) index patients without duodenal ulcer; III - 26 family members of 11 H. pylori (-) index patients. RESULTS: Seropositivity of group I and II (infected patients) was higher than the control group, 83% vs 38%, specially in mothers, 81% vs 18%, and in siblings 76% vs 20%. Differences between fathers' seropositivity was not statistically significant in the three groups: 100% vs 86% vs 70%. Seropositivity of all family members (mother, father and siblings) between infected group (I vs II) was similar. CONCLUSION: Prevalence of H. pylori infection was higher in family members of infected patients, but was similar among family members of infected patients with and without duodenal ulcer. H. pylori infection is more frequent in mothers and siblings of infected index children. A common source of infection cannot be excluded, but facts suggest that person-to-person transmission occurs, specially from mother to child.

scholarly journals Identification of Helicobacter pylori DNA in Iranian patients with gallstones

2004 ◽  
Vol 132 (6) ◽  
pp. 1185-1189 ◽  
Author(s):  
SH. FARSHAD ◽  
A. ALBORZI ◽  
S. A. MALEK HOSSEINI ◽  
B. OBOODI ◽  
M. RASOULI ◽  
...  
Keyword(s):  
Gallstone Formation ◽  
Control Group ◽  
Rapid Urease Test ◽  
Biliary Disease ◽  
Clinical Role ◽  
Test Culture ◽  
Urease Test ◽  
H Pylori ◽  
Bile Samples ◽  
Iranian Patients

In order to identify Helicobacter in gallstones of Iranian patients with biliary disease, gallstone and bile samples from 33 patients were subjected to rapid urease test, culture and Multiplex PCR using primers based on 16s rRNA and isocitrate dehydrogenase genes for the identification of Helicobacter genus and H. pylori respectively. This PCR was also done on bile samples from 40 autopsied gallbladders with normal pathology (control group). In 18·1% of stone and 12·1% of bile samples, H. pylori DNA was detected using PCR. Rapid urease and culture tests were negative for all samples. The PCR was negative in the control group. In conclusion, H. pylori DNA was detected in stone samples of Iranian patients with gallstones but we are not sure of their viability. To clarify the clinical role of Helicobacter in gallbladder diseases, studies using accurate tests on larger patient and control groups are needed to ascertain whether this microorganism is an innocent bystander or active participant in gallstone formation.

scholarly journals Use of a Gastric Juice-Based PCR Assay To DetectHelicobacter pylori Infection in Culture-Negative Patients

1998 ◽  
Vol 36 (1) ◽  
pp. 317-320 ◽  
Author(s):  
Haruhiko Yoshida ◽  
Katsutaro Hirota ◽  
Yasushi Shiratori ◽  
Takeshi Nihei ◽  
Shin Amano ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Gastric Juice ◽  
Rapid Urease Test ◽  
Pcr Assay ◽  
Gastroduodenal Disease ◽  
Urease Test ◽  
Conventional Tests ◽  
Conventional Test ◽  
H Pylori ◽  
Culture Negative

A gastric juice-based PCR assay was compared with culture, microscopy, and a rapid urease test with specimens from 114 subjects. The PCR and conventional tests were positive for 76 and 62% of the subjects, respectively. The prevalence of gastroduodenal disease and seropositivity for anti-Helicobacter pylori immunoglobulin G were similarly high among conventional-test-positive and PCR-only-positive subjects compared to all-negative ones. The PCR assay is recommended to confirm the H. pylori status of culture-negative peptic-ulcer patients.

scholarly journals DIAGNOSTIC UTILITY OF THE RAPID UREASE TEST FOR HELICOBACTER PYLORI IN GASTRIC JUICE SAMPLES

2021 ◽  
Vol 9 (08) ◽  
pp. 32-36
Author(s):  
Marlene Ordonez Pereira ◽  
◽  
Julian David Martinez ◽  
Keyword(s):  
Helicobacter Pylori ◽  
Gastric Juice ◽  
Diagnostic Tests ◽  
Diagnostic Performance ◽  
Medical Center ◽  
Rapid Urease Test ◽  
Test Device ◽  
Cross Sectional ◽  
Urease Test ◽  
H Pylori

Objective: The purpose of this study is to compare the diagnostic performance of the rapid urease test for H. pylori in endoscopic samples of gastric juice compared with the same test in gastric mucosa. Materials and methods: Cross-sectional, comparative and prospective study with study of concordance of diagnostic tests, carried out with patients referred to a medical center in Bogotá DC, Colombia for the performance of an esophagogastroduodenoscopy. We included 130 patients older than 18 years, without antibiotic treatment or inhibitors of gastric secretion, or with any type of immunodeficiency, or cancer. Were processed in the sensibacterpyroli test device a sample of gastric antrum for Rapid Ureasa Test RUT in mucosa and for the sample of juice 5 mL was taken with suctionator 7A-23B pulmomed (USA) R, to compare results. Results: In this study the infection by Helicobacter pylori was detected by biopsy sample in 40.8% of the patients that corresponded to 53 patients, among (59.2%) 77 patients with negative values. The prevalence of H. pylori infection in this study was 40.8%. It was calculated of VPP and NPV: 95% and NPV: 89.3%. Conclusions: rapid urease test in gastric juice is a fast and economic test, with very good sensitivity and specificity, is also very useful to detect H. pylori infection.

scholarly journals Diagnostic Performance of Gastric Imprint Smear for Determination ofHelicobacter pyloriInfection

2010 ◽  
Vol 24 (10) ◽  
pp. 603-606 ◽  
Author(s):  
J Al-Ali ◽  
F Al-Asfar ◽  
R Dhar ◽  
PM Dhar ◽  
K Kapila
Keyword(s):  
Diagnostic Performance ◽  
Rapid Test ◽  
Reference Method ◽  
Upper Gastrointestinal Endoscopy ◽  
Rapid Urease Test ◽  
Imprint Cytology ◽  
Culture Methods ◽  
Microbiological Methods ◽  
Urease Test ◽  
H Pylori

BACKGROUND: Despite the availability of several methods (invasive and noninvasive) for the diagnosis ofHelicobacter pyloriinfection, no test is considered to be the ‘gold standard’. Endoscopy-based tests are regarded as the reference method in most studies.OBJECTIVE: To evaluate the diagnostic performance of imprint cytology smears of antral biopsies compared with Gram-stained smears, the rapid urease test and culture methods, separately and in combination.METHODS: Antral biopsies were obtained from consecutive patients undergoing upper gastrointestinal endoscopy at a single centre. The biopsies were examined for the presence ofH pyloriby Gram-stained smear, the rapid urease test, culture methods and imprint cytology smear.RESULTS: A total of 273 biopsies were studied. All tests were positive in 36% of the patients. Of 252 biopsies tested, 73% were positive using the imprint cytology technique. Using Gram-stained smear, the rapid urease test and culture methods individually, the sensitivity and specificity of imprint cytology smears for the detection ofH pyloriwere found to be 92.7% and 50%; 92.7% and 49%; and 92.4% and 38.5%, respectively. Combining the three microbiological methods resulted in a sensitivity of 92.1%, a specificity of 51.0% and an efficiency of 71.7% for imprint cytology smears.CONCLUSIONS: Endoscopic examination provides useful clinical information. Imprint gastric cytology can be used as a rapid test to establish the diagnosis ofH pyloriinfection at the time endoscopy is performed, enabling the endoscopist to start treatment with immediate effect.

Sign in / Sign up

Export Citation Format

Share Document