scholarly journals Relevance of examination and treatment of the most common gastrointestinal disorders in children in Lithuania during the last decade

Medicina ◽  
2007 ◽  
Vol 44 (1) ◽  
pp. 72 ◽  
Author(s):  
Liutauras Labanauskas ◽  
Rūta Kučinskienė ◽  
Vaidotas Urbonas ◽  
Rūta Rokaitė ◽  
Neringa Libikaitė
Keyword(s):  
Duodenal Ulcer ◽  
Atopic Dermatitis ◽  
Abdominal Pain ◽  
Skin Rash ◽  
Endoscopic Examination ◽  
Gastrointestinal Disorders ◽  
Control Group ◽  
Rapid Urease Test ◽  
Urease Test ◽  
H Pylori

In the last decade, scientific studies in the field of children’s gastroenterology performed in Lithuania explored different problems: pathology of Helicobacter pylori infection and food allergy. Our studies Helirevealed that children with atopic dermatitis had gastrointestinal complaints (abdominal pain, diarrhea, distension and unstable stool, which appeared with the exacerbation of skin rash) more often as compared to nonallergic children of the control group. Abdominal pain in children with atopic dermatitis with local rash was more frequent and lasted longer than in control group children, whereas children with extended rash had stools more frequently. Gastrointestinal disorders in children with atopic dermatitis statistically significantly did not depend on the extent of skin rash and severity of atopic dermatitis. In our scientific research on the importance of H. pylori infection on children’s gastrointestinal system, children with chronic dyspepsia were examined. Endoscopy, rapid urease test, biopsies from antrum and corpus of stomach and their histological examination as well as serologic tests were done. According to the results obtained, we recommend to examine children with chronic dyspepsia in a complex way: not only endoscopic examination, but H. pylori diagnostic tests should be performed as well. Serologic test is not suitable for screening H. pylori infection in children. Considering this, we recommend to use no fewer than two different methods to diagnose this infection. The highest frequency of H. pylori infection was found in children with duodenal ulcer; histological changes in their gastric pylorus and corpus mucosa were greatest. More than half of children with nonulcer dyspepsia were infected with H. pylori. After eradication of H. pylori infection, the prevalence of dyspepsia in children with duodenal ulcer decreased.

scholarly journals Prevalence of Helicobacter pylori among patients with different gastrointestinal disorders in Saudi Arabia

2017 ◽  
Vol 25 (4) ◽  
pp. 214-20 ◽  
Author(s):  
Mohammed S. Alhussaini
Keyword(s):  
Duodenal Ulcer ◽  
Helicobacter Pylori ◽  
Saudi Arabia ◽  
Strong Association ◽  
Endoscopic Examination ◽  
Rapid Urease Test ◽  
Microscopical Examination ◽  
Increased Risk ◽  
Urease Test ◽  
H Pylori

Background: Helicobacter pylori is an important gastrointestinal pathogen associated with gastritis, peptic ulcers, and an increased risk of gastric carcinoma. The present study was carried out to determine the relationship between this organism with different gastrointestinal ailments.Methods: 150 outpatients referrals to Saudi Arabian Medical City, Riyadh, Kingdom of Saudi Arabia was recruited in January to June 2015. Each patient was subjected to endoscopic examination. Biopsy specimens were taken from the stomach for rapid urease test and culture. Suspected H. pylori colonies were subjected to colony morphology identification, microscopical examination and biochemical reactions. The samples were also subjected to PCR to detect ureA subunit of urease gene.Results: The endoscopic examination of patients revealed normal, gastric ulcer, duodenal ulcer, gastritis, and gastric cancer with a rate of 20.7%, 20%, 24%, 33.3%, and 2%, respectively. Direct smear exam revealed that 52% of patients were H. pylori positive while culture and rapid urease test showed a prevalence of 71.33%. Fifty four biopsies (36%) were urease positive after 1 hour at room temperature, 39 (62%) after 1 hour incubation at 37°C and 14 (71.33%) after 24 hours incubation. Isolated H. pylori showed that they were catalase, oxidase, and urease positive. PCR results showed 411-bp fragment, which is indicative for the ureA subunit of urease gene.Conclusion: The prevalence of H. pylori infection was high among tested population. Strong association between H. pylori and duodenal ulcer was noticed. A 411-bp fragment indicative of the ureA subunit of urease gene was detected in all the tested isolates.

scholarly journals Evidence of mother-child transmission of Helicobacter pylori infection

2004 ◽  
Vol 41 (4) ◽  
pp. 239-244 ◽  
Author(s):  
Mario Luis Escobar ◽  
Elisabete Kawakami
Keyword(s):  
Duodenal Ulcer ◽  
Helicobacter Pylori ◽  
Family Members ◽  
Control Group ◽  
Common Source ◽  
Rapid Urease Test ◽  
Source Of Infection ◽  
Group I ◽  
Urease Test ◽  
H Pylori

BACKGROUND: Low socioeconomical status is a major risk factor for natural acquisition of Helicobacter pylori (H. pylori) infection in developing countries. Its transmission route is unknown but studies suggest person-to-person transmission. AIM: To evaluate seropositivity of anti-H. pylori antibodies in family members of infected symptomatic index patients as compared to family members of symptomatic uninfected index patients. PATIENTS AND METHODS: One hundred and twelve family members of 38 patients who underwent endoscopy to exclude peptic disease were studied. Patients were deemed H. pylori infected or not infected when rapid urease test and histology were both positive or both negative. The family members underwent ELISA serology using the Cobas Core II Kit (Roche) and were classified into three groups: I - 29 family members of 10 H. pylori (+) duodenal ulcer index patients; II - 57 family members of 17 H. pylori (+) index patients without duodenal ulcer; III - 26 family members of 11 H. pylori (-) index patients. RESULTS: Seropositivity of group I and II (infected patients) was higher than the control group, 83% vs 38%, specially in mothers, 81% vs 18%, and in siblings 76% vs 20%. Differences between fathers' seropositivity was not statistically significant in the three groups: 100% vs 86% vs 70%. Seropositivity of all family members (mother, father and siblings) between infected group (I vs II) was similar. CONCLUSION: Prevalence of H. pylori infection was higher in family members of infected patients, but was similar among family members of infected patients with and without duodenal ulcer. H. pylori infection is more frequent in mothers and siblings of infected index children. A common source of infection cannot be excluded, but facts suggest that person-to-person transmission occurs, specially from mother to child.

scholarly journals Comparing Multiplex PCR and Rapid Urease Test in the Detection ofH. pyloriin Patients on Proton Pump Inhibitors

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Thomas Chen ◽  
Xiangwen Meng ◽  
H. Zhang ◽  
Rebecca W. Tsang ◽  
Tat-Kin Tsang
Keyword(s):  
Multiplex Pcr ◽  
Diagnostic Value ◽  
Control Group ◽  
Rapid Urease Test ◽  
Negative Results ◽  
Multiplex Pcr Assay ◽  
Time Period ◽  
Urease Test ◽  
H Pylori ◽  
The One

Background. This study was conducted to assess the diagnostic value of a multiplex PCR assay to detectH. pyloriinfection and to further evaluate the negative results from the CLOtest on patients with and without PPI treatment. Methods. This study is a retrospective cohort that included 457 patients with symptoms of dyspepsia, who underwent upper endoscopy at Evanston and Glenbrook Northshore Hospital from June 2003 to October 2007. A total of 556 samples were reported with some patients having more than one test over the time period. The CLOtest was performed first on the gastric specimen and from that specimen, the DNA was isolated and the one-step multiplex PCR was performed.Results. By M-PCR testing, H. pyloriwas detected in 143 (52.2%) of 274 cases in the control group and 130 (46.1%) of 282 cases in patients on PPI treatment (P=0.1746). The CLOtest detected the presence ofH. pyloriin 4 (1.4%) of 282 cases from the same group receiving PPI treatment and 29 (10.6%) of 274 cases from the group not taking a PPI (P≤0.0001).Conclusion. Our PCR is sensitive enough to detect the presence ofH. pyloridespite being on PPI treatment.

scholarly journals Endoscopic finding and treatment outcome of children with Helicobacter pylori infection in lower northern Thailand

2019 ◽  
Vol 13 (2) ◽  
pp. 79-82
Author(s):  
Chayakamon Niyasom ◽  
Thitima Ngoenmak ◽  
Suwannee Uthaisangsook
Keyword(s):  
Helicobacter Pylori ◽  
Abdominal Pain ◽  
Treatment Outcome ◽  
High Specificity ◽  
Chronic Abdominal Pain ◽  
Endoscopic Finding ◽  
Rapid Urease Test ◽  
Standard Regimen ◽  
Urease Test ◽  
H Pylori

Abstract Background Helicobacter pylori infection is one of the predisposing factors for gastritis, peptic ulcer, and duodenal ulcer. Definite diagnosis of H. pylori infection is important in planning effective medical management. However, confirming the diagnosis through bacterial culture takes a number of days, and thus delays treatment. Objectives To examine endoscopic findings in children associated with chronic abdominal pain and H. pylori infection to aid in early diagnosis. We also evaluated treatment outcome of H. pylori infection. Methods A retrospective study was performed by reviewing the medical records of children under 15 years of age with chronic abdominal pain who underwent esophagogastroduodenoscopy (EGD) between 2011 and 2017. According to 2016 Joint ESPGHAN/NASPGHAN Guidelines, H. pylori infection was defined by positive tests for both histopathology test and rapid urease test (RUT). The EGD finding, RUT, histopathologic finding, and treatment outcome were recorded. Results Forty-eight children presented with chronic abdominal pain (male 47.9%, female 52.1%, mean age was 8.44 ± 2.97 years). Twelve children out of 48 had H. pylori infection (12/48, 25%). Eight among the 12 children had antral nodularity (8/12), wherein there was no antral nodularity in children without H. pylori infection (0/36). This difference was statistically significant (P < 0.001). Sensitivity and specificity of antral nodularity finding for H. pylori infection were 66.7% and 100%, respectively. Eradication of H. pylori infection with standard regimen improved the abdominal pain within 4 weeks. Conclusion The occurrence of antral nodularity in endoscopic finding was significantly associated with H. pylori infection. In addition, antral nodularity finding showed a good sensitivity and high specificity for the diagnosis of H. pylori infection.

A new understanding of the H. pylori eradication mechanism

2019 ◽  
Author(s):  
Shanshan Su ◽  
Guo-qi Zheng ◽  
Ying-ying Liu ◽  
Yu-fei Liang ◽  
Hui Song ◽  
...  
Keyword(s):  
Gastric Juice ◽  
Intervention Group ◽  
Control Group ◽  
Rapid Urease Test ◽  
Mucus Layer ◽  
Culture Methods ◽  
Detection Rates ◽  
Urease Test ◽  
Gastric Lumen ◽  
H Pylori

Abstract Background: Helicobacter pylori (H. pylori) cannot usually be detected in the gastric juice and it is thought that H. pylori may be implanted under the mucus layer for long term. The mechanisms of action of proton pump inhibitor (PPI), antibiotics, and bismuth for H. pylori eradication are not entirely clear. Our study aimed to determine the role of PPI on the movement of H. pylori across the mucus layer to the gastric lumen and the mechanism of PPI, antibiotics, and bismuth on H. pylori eradication. Methods: Patients with H. pylori infection were intravenous injected with PPI (intervention group, n=31) or without PPI (control group, n=37). The presence of H. pylori in the gastric juice was evaluated by the rapid urease test (RUT), polymerase chain reaction (PCR), and culture methods. Results: The H. pylori positive detection rates were all significantly higher among patients in the intervention group than among patients in the control group by the RUT (P < 0.0001), PCR (P < 0.0001), and culturing (P = 0.0386). Conclusion: H. pylori can penetrate across the mucus layer to the gastric lumen following PPI intervention. The direct antimicrobial activity of PPI might because of diminished numbers of H. pylori due to probiotics in the gastric lumen. Antibiotics and bismuth might play a local sterilization role in the gastric lumen when H. pylori penetrate across the mucus layer.

scholarly journals Association of TP53 gene codon 72 polymorphism with Helicobacter pylori-positive non-cardia gastric cancer in Vietnam

2019 ◽  
Vol 13 (11) ◽  
pp. 984-991
Author(s):  
Thi Minh Thi Ha ◽  
Thanh Nha Uyen Le ◽  
Viet Nhan Nguyen ◽  
Van Huy Tran
Keyword(s):  
Gastric Cancer ◽  
Control Group ◽  
Rapid Urease Test ◽  
Genotype Distribution ◽  
Codon 72 ◽  
Codon 72 Polymorphism ◽  
Urease Test ◽  
Significant Difference ◽  
Tp53 Codon 72 Polymorphism ◽  
H Pylori

Introduction: This research aimed to determine the association of the combination of H. pylori infection and TP53 codon 72 polymorphism with non-cardia gastric cancer (GC) in Vietnam. Methodology: A total of 164 patients with non-cardia GC and 164 patients with peptic ulcer disease or functional dyspepsia in controls matched by sex and age were enrolled. H. pylori infection was diagnosed by rapid urease test and polymerase chain reaction (PCR). The cagA gene-positivity and vacA sm subtypes were determined by multiplex PCR. Genotypes of TP53 codon 72 polymorphism were determined by PCR-restriction fragment length polymorphism. Results: The prevalence of H. pylori infection in GC and control group were 61.6% and 55.4%, respectively. The rates of cagA-positive strains in the two H. pylori-positive groups were 80.2% and 71.4%, respectively. There was no statistically significant difference in TP53 codon 72 genotype distribution between GC group (frequencies of Arg/Arg, Arg/Pro and Pro/Pro genotypes were 31.1%, 43.3% and 25.6%, respectively) and controls (29.3%, 52.4% and 18.3%, respectively), p = 0.172. The significant difference in genotype distribution was observed in recessive model (Pro/Pro vs Arg/Arg + Arg/Pro) when stratifying by H. pylori infection (OR = 2.02, 95% CI 1.03–3.96, p = 0.041) and by cagA-positivity (OR = 2.33, 95% CI 1.07–5.07, p = 0.032). Conclusions: This study suggests a synergistic interaction between H. pylori infection, especially cagA-positive H. pylori, and Pro/Pro genotype of TP53 codon 72 polymorphism might play a significant role in the pathogenesis of GC in the Vietnamese population.

scholarly journals H.PYLORI INFECTION

2016 ◽  
Vol 23 (03) ◽  
pp. 307-311
Author(s):  
Shabnam Naveed ◽  
Syed Masroor Ahmed ◽  
Zeeshan Ali ◽  
Romana Awan ◽  
Humaira Zakir ◽  
...  
Keyword(s):  
Duodenal Ulcer ◽  
Gastric Ulcer ◽  
Cross Sectional Study ◽  
Rapid Urease Test ◽  
Viral Etiology ◽  
Cross Sectional ◽  
Urease Test ◽  
Cirrhotic Patients ◽  
H Pylori ◽  
Study Setting

Objectives: To study the frequency of H. pylori infection and its association as acause of upper G.I bleeding in cirrhosis. Study Design: Cross Sectional Study. Setting: MedicalUnit III, JPMC Karachi. Period: January 2014 to December 2014. Methods: 100 patients ofestablished cirrhosis with upper G.I bleeding were included in this study. These patients wereevaluated for viral etiology of cirrhosis. An upper G.I Endoscopy was performed in all patientsand antral biopsies were taken. Rapid urease test was performed on biopsy specimen fordetection of H.pylori infection. Results: Out of 100 patients 71 were males and 29 were femaleswith age ranging between 14 to 70 years. Among them 53% patients turned out to be positivefor H.Pylori infection with rapid urease test. H.pylori infection was detected in 68.7% of HBsAgpositive patients and in 50% of Anti HCV positive patients. On upper G.I endoscopy 96 patientshad PHG and among them 50 (52%) were positive for H.pylori. 4 patients did not have PHGand among them 3 (75%) were positive for H.pylori. Gastric ulcer was present in 17 patientsand amongst them H.pylori was detected in 10 (58.8%) cases. Duodenal ulcer was present in 5patients and among them 2 (40%) were positive for H.pylori. Gastritis was present in 17 casesamong them 11 (64%) were positive for H.pylori. Duodenitis was present in 13 cases amongthem 11 (84.6%) patients were positive for H.pylori infection. Conclusion: The frequency ofH.pylori infection was low in cirrhotic patients. No association was seen in H.pylori infectionand causes of upper G.I bleeding in cirrhosis including PHG, gastric ulcer and duodenal ulcer

scholarly journals Identification of Helicobacter pylori DNA in Iranian patients with gallstones

2004 ◽  
Vol 132 (6) ◽  
pp. 1185-1189 ◽  
Author(s):  
SH. FARSHAD ◽  
A. ALBORZI ◽  
S. A. MALEK HOSSEINI ◽  
B. OBOODI ◽  
M. RASOULI ◽  
...  
Keyword(s):  
Gallstone Formation ◽  
Control Group ◽  
Rapid Urease Test ◽  
Biliary Disease ◽  
Clinical Role ◽  
Test Culture ◽  
Urease Test ◽  
H Pylori ◽  
Bile Samples ◽  
Iranian Patients

In order to identify Helicobacter in gallstones of Iranian patients with biliary disease, gallstone and bile samples from 33 patients were subjected to rapid urease test, culture and Multiplex PCR using primers based on 16s rRNA and isocitrate dehydrogenase genes for the identification of Helicobacter genus and H. pylori respectively. This PCR was also done on bile samples from 40 autopsied gallbladders with normal pathology (control group). In 18·1% of stone and 12·1% of bile samples, H. pylori DNA was detected using PCR. Rapid urease and culture tests were negative for all samples. The PCR was negative in the control group. In conclusion, H. pylori DNA was detected in stone samples of Iranian patients with gallstones but we are not sure of their viability. To clarify the clinical role of Helicobacter in gallbladder diseases, studies using accurate tests on larger patient and control groups are needed to ascertain whether this microorganism is an innocent bystander or active participant in gallstone formation.

scholarly journals Prevalence of helicobacter pylori infection in patients with peptic ulcer disease at Kathmandu Model Hospital

2018 ◽  
Vol 8 (4) ◽  
pp. 3-7
Author(s):  
Rajesh Dhoj Joshi ◽  
Sachin Khadka ◽  
Deepak Man Joshi ◽  
Arun Kadel ◽  
Ganesh Dangal ◽  
...  
Keyword(s):  
Duodenal Ulcer ◽  
Peptic Ulcer ◽  
Peptic Ulcer Disease ◽  
Rapid Urease Test ◽  
Gi Endoscopy ◽  
Ulcer Disease ◽  
Upper Gi ◽  
Urease Test ◽  
Upper Gi Endoscopy ◽  
H Pylori

Introduction: Endoscopic rapid urease test is a simple and most widely used test to detect the presence of urease in the gastric mucosa. Many studies have reported prevalence of H. pylori infection in relation to age, gender and site of ulcer. Therefore, this study was designed to determine the prevalence and significance of H. pylori in peptic ulcer disease. Methods: A retrospective review was carried out for patients with peptic ulcer disease who had undergone upper GI endoscopy in Department of Internal Medicine at Kathmandu Model Hos­pital. The records from January 2013 to December 2017 were analyzed. Any patient with previously diagnosed peptic ulcer, history of active bleeding, cancer and incomplete records were excluded. Peptic ulcer associated with H. pylori was diagnosed on the basis of endoscopic rapid urease test. Results: Among the 418 diagnosed case of peptic ulcer disease by upper GI endoscopy from Jan 2013 to Dec 2017, 213 tested positive for H. pylori by rapid urease test. Among the positive cases, over a half were males patients. Majority (23.9%) of the patients were in the age group of 35-44 years. Prevalence of H. pylori in duodenal ulcer was 51.6% followed by combined gastro-duodenal ulcer (26.8%) and gastric ulcer (21.6%). H. pylori was significantly associated with duodenal ulcer (p<0.0001). Conclusion: This study demonstrated relatively high prevalence of H. pylori infection in patients with duodenal ulcer who had undergone upper GI endoscopy.

scholarly journals The Differences Level of Resistin between Positive and Negative Helicobacter pylori Gastritis and its Correlation with Nutritional Status

2020 ◽  
Vol 21 (2) ◽  
pp. 114-119
Author(s):  
Widya Deli Satuti ◽  
Gontar Alamsyah Siregar ◽  
Dharma Lindarto ◽  
Imelda Rey
Keyword(s):  
Body Mass Index ◽  
Waist Circumference ◽  
Body Mass ◽  
Strong Relationship ◽  
Endoscopic Examination ◽  
Rapid Urease Test ◽  
Strong Positive Correlation ◽  
Cross Sectional ◽  
Urease Test ◽  
H Pylori

Background: H. pylori gastritis can be related to people with obesity and metabolic syndrome. The mechanism underlying the relationship between them is suspected to be caused by adipocytokine called resistin. Objective: to analyze the difference level of resistin between positive and negative H. pylori gastritis patients and their relationship with nutritional status.Method: This cross sectional study was done in the Gastroenterology department of General Hospital Adam Malik, Medan, from May to October 2019. Patients with gastritis diagnosed by endoscopy underwent rapid urease test (RUT), resistin, albumin, hemoglobin tests, also body mass index (BMI) and waist circumference (WC) were measured.Results: Sixty gastritis patients found by endoscopic examination, 29 (48.33%) was H. pylori positive and 31 (51.67%) was negative. No significant differences were found between BMI, WC, and albumin against H. pylori gastritis with p values 0.099, 0.055, and 0.528, respectively. Hemoglobin was found significant with a p 0.009. The mean resistin in the positive H. pylori group was 7.62 ng/ml while the negative was 3.23 ng/ml, and statistically significant (p 0.001). There was a strong positive correlation between resistin levels with BMI and WC (BMI p 0.01, r 0.577; WC p 0.001, r 0.592).Conclusion: Resistin levels in gastritis with H. pylori positive are higher than H. pylori negative, body mass index and waist circumference have a strong relationship with resistin levels in patients with positive H. pylori gastritis, hemoglobin is lower in H. pylori positive than negative gastritis.

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