scholarly journals A member of wheat class III peroxidase gene family, named TaPRX-2A, enhanced the tolerance of salt stress

2020 ◽  
Author(s):  
Peisen Su ◽  
Jun Yan ◽  
Wen Li ◽  
Liang Wang ◽  
Jinxiao Zhao ◽  
...  
Keyword(s):  
Salt Stress ◽  
Stress Response ◽  
Gene Family ◽  
Abiotic Stresses ◽  
Transgenic Wheat ◽  
Class Iii ◽  
Salt Stress Response ◽  
Peroxidase Gene ◽  
Stress Related Genes ◽  
Class Iii Peroxidase

Abstract Background: Abiotic stresses including salt stress are environment stresses of limiting the crop growth and yield. It was reported that peroxidases (PRX) were involved in various abiotic stress responses. However, few wheat PRXs were characterized in the mechanism of abiotic stresses. Results: In this study, a novel wheat PRX gene named TaPRX-2A, a member of wheat class III peroxidase gene family, was cloned and characterized in salt stress response. According to the identification of class III PRXs in 12 different plants, we proposed an evolutionary model that this TaPRX-2A may have experienced some exon fusion events during evolution. The results of expression pattern showed that TaPRX-2A exhibited relatively high expression levels in root tissue, but low in stem and leaf tissues by using qRT-PCR. This TaPRX-2A was also induced by some stress and hormone treatments including PEG6000, NaCl, H 2 O 2 , SA, JA, and ABA. The result of overexpressing transgenic wheat showed that this TaPRX-2A enhanced the tolerance of salt comparing the wild-type wheat (WT). We also studied the molecular mechanism of TaPRX-2A mediating the salt stress response. Physiological experiments indicated that TaPRX-2A -overexpressing transgenic wheat possessed a higher survival rate, higher relative water content, and longer shoot length than WT, but remained the same in the root length under salt stress. Further experiments indicated that TaPRX-2A -overexpressing transgenic lines enhanced abiotic tolerance by enhancing oxidative stress tolerance, such as higher antioxidant activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) enzymes, reduction of reactive oxygen species (ROS) accumulation, and lower levels of MDA content. Moreover, the transcript levels of stress-related genes were up-regulated by overexpression of TaPRX-2A. Conclusions: The results showed that TaPRX-2A play a positive factor in response to salt stress by scavenging ROS and regulating stress-related genes.

scholarly journals A member of wheat class III peroxidase gene family, TaPRX-2A, enhanced the tolerance of salt stress

2020 ◽  
Author(s):  
Peisen Su ◽  
Jun Yan ◽  
Wen Li ◽  
Liang Wang ◽  
Jinxiao Zhao ◽  
...  
Keyword(s):  
Salt Stress ◽  
Gene Family ◽  
Abiotic Stresses ◽  
Evolutionary Model ◽  
Transgenic Wheat ◽  
Class Iii ◽  
Expression Levels ◽  
Peroxidase Gene ◽  
Stress Related Genes ◽  
Class Iii Peroxidase

Abstract Background: Salt and drought are the main abiotic stresses that restrict yield of crops. It is reported that peroxidases (PRXs) are involved in various abiotic stress responses. However, few wheat PRXs are characterized in the mechanism of abiotic stresses. Results: In this study, a novel wheat peroxidase (PRX) gene named TaPRX-2A, a member of wheat class III peroxidase gene family, was cloned and characterized in salt stress response. Based on the identification and evolutionary analysis of class III PRXs in 12 plants, we proposed an evolutionary model that TaPRX-2A might have experienced some exon fusion events during evolution. We also detected the positive selection of PRX domain in 13 PRXs involving our evolutionary model, and found 2 or 6 positively selected sites during TaPRX-2A evolution. The results of expression pattern showed that TaPRX-2A exhibited relatively higher expression levels in root tissue compared with that of leaf and stem tissues by using qRT-PCR. This TaPRX-2A was also induced by some stresses and hormone treatments including PEG6000, NaCl, hydrogen peroxide (H 2 O 2 ), salicylic acid (SA), methyljasmonic acid (MeJA) and abscisic acid (ABA). Transgenic wheat plants with overexpression of TaPRX-2A showed higher tolerance to salt stress than wild type (WT) plants. Confocal microscopy revealed that TaPRX-2A :eGFP was mainly localized in nuclei. The survival rate, relative water content and shoot length were higher in TaPRX-2A -overexpressing wheat than WT. However, root lengths were no significant difference between transgenic wheat and WT. The activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) were enhanced in TaPRX-2A -overexpressing wheat than WT, resulting in the reduction of reactive oxygen species (ROS) accumulation and malondialdehyde (MDA) content. We also measured the expression levels of downstream stress-related genes ( RD22 , TLP4 , ABAI , GLP4 , GST22 , FeSOD, CuSOD, and CAT ). The results showed that RD22 , TLP4 , ABAI , GST22 , FeSOD, and CAT exhibited higher expression in TaPRX-2A -overexpressing lines than in WT under salt stress. Conclusions: The results show that TaPRX-2A plays a positive factor in response to salt stress by scavenging ROS and regulating stress-related genes.

scholarly journals A member of wheat class III peroxidase gene family, TaPRX-2A, enhanced the tolerance of salt stress

2020 ◽  
Author(s):  
Peisen Su ◽  
Jun Yan ◽  
Wen Li ◽  
Liang Wang ◽  
Jinxiao Zhao ◽  
...  
Keyword(s):  
Salt Stress ◽  
Abiotic Stress ◽  
Gene Family ◽  
Stress Responses ◽  
Abiotic Stresses ◽  
Evolutionary Model ◽  
Evolutionary Analysis ◽  
Class Iii ◽  
Expression Levels ◽  
Stress Related Genes

Abstract Background: Salt and drought are the main abiotic stresses that restrict the yield of crops. Peroxidases (PRXs) are involved in various abiotic stress responses. Furthermore, only few wheat PRXs have been characterized in the mechanism of the abiotic stress response.Results: In this study, a novel wheat peroxidase (PRX) gene named TaPRX-2A, a member of wheat class III PRX gene family, was cloned and its response to salt stress was characterized. Based on the identification and evolutionary analysis of class III PRXs in 12 plants, we proposed an evolutionary model for TaPRX-2A, suggesting that occurrence of some exon fusion events during evolution. We also detected the positive selection of PRX domain in 13 PRXs involving our evolutionary model, and found 2 or 6 positively selected sites during TaPRX-2A evolution. Quantitative reverse transcription–polymerase chain reaction (qRT–PCR) results showed that TaPRX-2A exhibited relatively higher expression levels in root tissue than those exhibited in leaf and stem tissues. TaPRX-2A expression was also induced by abiotic stresses and hormone treatments such as polyethylene glycol 6000, NaCl, hydrogen peroxide (H2O2), salicylic acid (SA), methyljasmonic acid (MeJA) and abscisic acid (ABA). Transgenic wheat plants with overexpression of TaPRX-2A showed higher tolerance to salt stress than wild-type (WT) plants. Confocal microscopy revealed that TaPRX-2A-eGFP was mainly localized in cell nuclei. Survival rate, relative water content, and shoot length were higher in TaPRX-2A-overexpressing wheat than in the WT wheat, whereas root length was not significantly different. The activities of s superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) were enhanced in TaPRX-2A-overexpressing wheat compared with those in the WT wheat, resulting in the reduction of reactive oxygen species (ROS) accumulation and malondialdehyde (MDA) content. The expression levels of downstream stress-related genes showed that RD22, TLP4, ABAI, GST22, FeSOD, and CAT exhibited higher expressions in TaPRX-2A-overexpressing wheat than in WT under salt stress.Conclusions: The results show that TaPRX-2A plays a positive role in the response to salt stress by scavenging ROS and regulating stress-related genes.

scholarly journals A member of wheat class III peroxidase gene family, TaPRX-2A, enhanced the tolerance of salt stress

2020 ◽  
Author(s):  
Peisen Su ◽  
Jun Yan ◽  
Wen Li ◽  
Liang Wang ◽  
Jinxiao Zhao ◽  
...  
Keyword(s):  
Salt Stress ◽  
Abiotic Stress ◽  
Gene Family ◽  
Stress Responses ◽  
Abiotic Stresses ◽  
Evolutionary Model ◽  
Evolutionary Analysis ◽  
Class Iii ◽  
Expression Levels ◽  
Stress Related Genes

Abstract Background: Salt and drought are the main abiotic stresses that restrict the yield of crops. Peroxidases (PRXs) are involved in various abiotic stress responses. Furthermore, only few wheat PRXs have been characterized in the mechanism of the abiotic stress response.Results: In this study, a novel wheat peroxidase (PRX) gene named TaPRX-2A, a member of wheat class III PRX gene family, was cloned and its response to salt stress was characterized. Based on the identification and evolutionary analysis of class III PRXs in 12 plants, we proposed an evolutionary model for TaPRX-2A, suggesting that occurrence of some exon fusion events during evolution. We also detected the positive selection of PRX domain in 13 PRXs involving our evolutionary model, and found 2 or 6 positively selected sites during TaPRX-2A evolution. Quantitative reverse transcription–polymerase chain reaction (qRT–PCR) results showed that TaPRX-2A exhibited relatively higher expression levels in root tissue than those exhibited in leaf and stem tissues. TaPRX-2A expression was also induced by abiotic stresses and hormone treatments such as polyethylene glycol 6000, NaCl, hydrogen peroxide (H2O2), salicylic acid (SA), methyljasmonic acid (MeJA) and abscisic acid (ABA). Transgenic wheat plants with overexpression of TaPRX-2A showed higher tolerance to salt stress than wild-type (WT) plants. Confocal microscopy revealed that TaPRX-2A-eGFP was mainly localized in cell nuclei. Survival rate, relative water content, and shoot length were higher in TaPRX-2A-overexpressing wheat than in the WT wheat, whereas root length was not significantly different. The activities of s superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) were enhanced in TaPRX-2A-overexpressing wheat compared with those in the WT wheat, resulting in the reduction of reactive oxygen species (ROS) accumulation and malondialdehyde (MDA) content. The expression levels of downstream stress-related genes showed that RD22, TLP4, ABAI, GST22, FeSOD, and CAT exhibited higher expressions in TaPRX-2A-overexpressing wheat than in WT under salt stress.Conclusions: The results show that TaPRX-2A plays a positive role in the response to salt stress by scavenging ROS and regulating stress-related genes.

scholarly journals A member of wheat class III peroxidase gene family, TaPRX-2A, enhanced the tolerance of salt stress

2020 ◽  
Author(s):  
Peisen Su ◽  
Jun Yan ◽  
Wen Li ◽  
Liang Wang ◽  
Jinxiao Zhao ◽  
...  
Keyword(s):  
Salt Stress ◽  
Abiotic Stress ◽  
Gene Family ◽  
Stress Responses ◽  
Abiotic Stresses ◽  
Evolutionary Model ◽  
Evolutionary Analysis ◽  
Class Iii ◽  
Expression Levels ◽  
Stress Related Genes

Abstract Background: Salt and drought are the main abiotic stresses that restrict the yield of crops. Peroxidases (PRXs) are involved in various abiotic stress responses. Furthermore, only few wheat PRXs have been characterized in the mechanism of the abiotic stress response.Results: In this study, a novel wheat peroxidase (PRX) gene named TaPRX-2A, a member of wheat class III PRX gene family, was cloned and its response to salt stress was characterized. Based on the identification and evolutionary analysis of class III PRXs in 12 plants, we proposed an evolutionary model for TaPRX-2A, suggesting that occurrence of some exon fusion events during evolution. We also detected the positive selection of PRX domain in 13 PRXs involving our evolutionary model, and found 2 or 6 positively selected sites during TaPRX-2A evolution. Quantitative reverse transcription–polymerase chain reaction (qRT–PCR) results showed that TaPRX-2A exhibited relatively higher expression levels in root tissue than those exhibited in leaf and stem tissues. TaPRX-2A expression was also induced by abiotic stresses and hormone treatments such as polyethylene glycol 6000, NaCl, hydrogen peroxide (H2O2), salicylic acid (SA), methyljasmonic acid (MeJA) and abscisic acid (ABA). Transgenic wheat plants with overexpression of TaPRX-2A showed higher tolerance to salt stress than wild-type (WT) plants. Confocal microscopy revealed that TaPRX-2A-eGFP was mainly localized in cell nuclei. Survival rate, relative water content, and shoot length were higher in TaPRX-2A-overexpressing wheat than in the WT wheat, whereas root length was not significantly different. The activities of s superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) were enhanced in TaPRX-2A-overexpressing wheat compared with those in the WT wheat, resulting in the reduction of reactive oxygen species (ROS) accumulation and malondialdehyde (MDA) content. The expression levels of downstream stress-related genes showed that RD22, TLP4, ABAI, GST22, FeSOD, and CAT exhibited higher expressions in TaPRX-2A-overexpressing wheat than in WT under salt stress.Conclusions: The results show that TaPRX-2A plays a positive role in the response to salt stress by scavenging ROS and regulating stress-related genes.

scholarly journals A member of wheat class III peroxidase gene family, TaPRX-2A, enhanced the tolerance of salt stress

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Peisen Su ◽  
Jun Yan ◽  
Wen Li ◽  
Liang Wang ◽  
Jinxiao Zhao ◽  
...  
Keyword(s):  
Salt Stress ◽  
Gene Family ◽  
Class Iii ◽  
Peroxidase Gene ◽  
Class Iii Peroxidase

Systematic analysis of maize class III peroxidase gene family reveals a conserved subfamily involved in abiotic stress response

Gene ◽  
2015 ◽  
Vol 566 (1) ◽  
pp. 95-108 ◽  
Author(s):  
Yu Wang ◽  
Qianqian Wang ◽  
Yang Zhao ◽  
Guomin Han ◽  
Suwen Zhu
Keyword(s):  
Abiotic Stress ◽  
Stress Response ◽  
Gene Family ◽  
Class Iii ◽  
Abiotic Stress Response ◽  
Systematic Analysis ◽  
Peroxidase Gene ◽  
Class Iii Peroxidase

scholarly journals Salicylic Acid Alleviated Salt Damage of Populus euphratica: A Physiological and Transcriptomic Analysis

Forests ◽  
2019 ◽  
Vol 10 (5) ◽  
pp. 423 ◽  
Author(s):  
Shupei Rao ◽  
Chao Du ◽  
Aijia Li ◽  
Xinli Xia ◽  
Weilun Yin ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Antioxidant Enzymes ◽  
Salt Stress ◽  
Stress Response ◽  
Protein Kinase ◽  
Abiotic Stresses ◽  
Growth And Development ◽  
Populus Euphratica ◽  
Mitogen Activated Protein Kinase ◽  
Salt Stress Response

Populus euphratica Oliv. is a model tree for studying abiotic stress, especially salt stress response. Salt stress is one of the most extensive abiotic stresses, which has an adverse effect on plant growth and development. Salicylic acid (SA) is an important signaling molecule that plays an important role in modulating the plant responses to abiotic stresses. To answer whether the endogenous SA can be induced by salt stress, and whether SA effectively alleviates the negative effects of salt on poplar growth is the main purpose of the study. To elucidate the effects of SA and salt stress on the growth of P. euphratica, we examined the morphological and physiological changes of P. euphratica under 300 mM NaCl after treatment with different concentrations of SA. A pretreatment of P. euphratica with 0.4 mM SA for 3 days effectively improved the growth status of plants under subsequent salt stress. These results indicate that appropriate concentrations of exogenous SA can effectively counteract the negative effect of salt stress on growth and development. Subsequently, transcripts involved in salt stress response via SA signaling were captured by RNA sequencing. The results indicated that numerous specific genes encoding mitogen-activated protein kinase, calcium-dependent protein kinase, and antioxidant enzymes were upregulated. Potassium transporters and Na+/H+ antiporters, which maintain K+/Na+ balance, were also upregulated after SA pretreatment. The transcriptome changes show that the ion transport and antioxidant enzymes were the early enhanced systems in response of P. euphratica to salt via SA, expanding our knowledge about SA function in salt stress defense in P. euphratica. This provides a solid foundation for future study of functional genes controlling effective components in metabolic pathways of trees.

scholarly journals The Arabidopsis HY2 Gene Acts as a Positive Regulator of NaCl Signaling during Seed Germination

2021 ◽  
Vol 22 (16) ◽  
pp. 9009
Author(s):  
Mingxin Piao ◽  
Jinpeng Zou ◽  
Zhifang Li ◽  
Junchuan Zhang ◽  
Liang Yang ◽  
...  
Keyword(s):  
Salt Stress ◽  
Stress Response ◽  
Seed Germination ◽  
Plant Stress ◽  
Ion Homeostasis ◽  
Enrichment Analysis ◽  
Salt Stress Response ◽  
Go Enrichment ◽  
Stress Related Genes ◽  
Go Enrichment Analysis

Phytochromobilin (PΦB) participates in the regulation of plant growth and development as an important synthetase of photoreceptor phytochromes (phy). In addition, Arabidopsis long hypocotyl 2 (HY2) appropriately works as a key PΦB synthetase. However, whether HY2 takes part in the plant stress response signal network remains unknown. Here, we described the function of HY2 in NaCl signaling. The hy2 mutant was NaCl-insensitive, whereas HY2-overexpressing lines showed NaCl-hypersensitive phenotypes during seed germination. The exogenous NaCl induced the transcription and the protein level of HY2, which positively mediated the expression of downstream stress-related genes of RD29A, RD29B, and DREB2A. Further quantitative proteomics showed the patterns of 7391 proteins under salt stress. HY2 was then found to specifically mediate 215 differentially regulated proteins (DRPs), which, according to GO enrichment analysis, were mainly involved in ion homeostasis, flavonoid biosynthetic and metabolic pathways, hormone response (SA, JA, ABA, ethylene), the reactive oxygen species (ROS) metabolic pathway, photosynthesis, and detoxification pathways to respond to salt stress. More importantly, ANNAT1–ANNAT2–ANNAT3–ANNAT4 and GSTU19–GSTF10–RPL5A–RPL5B–AT2G32060, two protein interaction networks specifically regulated by HY2, jointly participated in the salt stress response. These results direct the pathway of HY2 participating in salt stress, and provide new insights for the plant to resist salt stress.

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