Physiological and transcriptome analysis reveal molecular mechanism in Salvia miltiorrhiza leaves of near-isogenic male fertile lines and male sterile lines

Abstract Background: Our previous study found that male sterility in Salvia miltiorrhiza could result in stunted growth, reduced biomass, decreased primary metabolism, and increased secondary metabolism, but their molecular mechanisms have not yet been revealed. In this article, we investigated the underlying mechanism of male sterility to plant growth and metabolic yield by using physiological analysis and mRNA sequencing (RNA-Seq). Results: In this study, transcriptomic and physiological analysis were performed to identify the mechanism of male sterile mutants to plant growth and metabolic yield. Through Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, it was found that the pathways were mainly enriched in processes including organ development, primary metabolic process and secondary metabolic process. Physiological analysis showed that the chloroplast structure of male sterile mutants of S. miltiorrhiza was abnormally developed, which could result in decrease in leaf gas exchange (A, E and gs), chlorophyll fluorescence (Fv, Fm and Fv/Fm), and the chlorophyll content. Among the differentially expressed genes (DEGs), the expression levels of 7 genes involved to photosynthesis-related pathway were down-regulated in male sterile lines of S. miltiorrhiza, which was consistent with the corresponding phenotypic changes in chlorophyll fluorescence, chlorophyll content and leaf gas exchange. Transcriptomic analysis established the role of disproportionating enzyme 1 (DPE1) as catalyzing the degradation of starch, and the role sucrose synthase 3 (SUS3) and cytosolic invertase 2 (CINV2) as catalyzing the degradation of sucrose in the S. miltiorrhiza mutants. The results also confirmed that phenylalanine ammonialyase (PAL) was involved in the biosynthesis of rosmarinic acid and salvianolic acid B, and flavone synthase (FLS) was an important enzyme catalyzing steps of flavonoid biosynthesis. Conclusions: Our results from the physiological and transcriptome analysis reveal underlying mechanism of plant growth and metabolic yield in male sterile mutants, and provide insight into the crop yield of S. miltiorrhiza.

Download Full-text

Physiological and transcriptome analysis reveal molecular mechanism in Salvia miltiorrhiza leaves of near-isogenic male fertile lines and male sterile lines

10.21203/rs.2.9785/v3 ◽

2019 ◽

Author(s):

Ruihong Wang ◽

Han Jiang ◽

Ziyun Zhou ◽

Hongbo Guo ◽

Juane Dong

Keyword(s):

Chlorophyll Fluorescence ◽

Plant Growth ◽

Male Sterility ◽

Leaf Gas Exchange ◽

Salvia Miltiorrhiza ◽

Underlying Mechanism ◽

Metabolic Process ◽

Male Sterile ◽

Physiological Analysis

Abstract Background: Our previous study finds that male sterility in Salvia miltiorrhiza could result in stunted growth and reduced biomass, but their molecular mechanisms have not yet been revealed. In this article, we investigate the underlying mechanism of male sterility and its impact on plant growth and metabolic yield by using physiological analysis and mRNA sequencing (RNA-Seq). Results: In this study, transcriptomic and physiological analysis were performed to identify the mechanism of male sterility in mutants and its impact on plant growth and metabolic yield. Through Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, it is found that the pathways are mainly enriched in processes including organ development, primary metabolic process and secondary metabolic process. Physiological analysis show that the chloroplast structure of male sterile mutants of S. miltiorrhiza is abnormally developed, which could result in decrease in leaf gas exchange ( A , E and gs ), chlorophyll fluorescence (Fv, Fm and Fv/Fm), and the chlorophyll content. Expression level of 7 differentially expressed genes involved in photosynthesis-related pathways is downregulated in male sterile lines of S. miltiorrhiza , which could explain the corresponding phenotypic changes in chlorophyll fluorescence, chlorophyll content and leaf gas exchange. Transcriptomic analysis establishes the role of disproportionating enzyme 1 ( DPE1 ) as catalyzing the degradation of starch, and the role of sucrose synthase 3 ( SUS3 ) and cytosolic invertase 2 ( CINV2 ) as catalyzing the degradation of sucrose in the S. miltiorrhiza mutants. The results also confirm that phenylalanine ammonialyase ( PAL ) is involved in the biosynthesis of rosmarinic acid and salvianolic acid B, and flavone synthase ( FLS ) is an important enzyme catalyzing steps of flavonoid biosynthesis. Conclusions: Our results from the physiological and transcriptome analysis reveal underlying mechanism of plant growth and metabolic yield in male sterile mutants, and provide insight into the crop yield of S. miltiorrhiza.

Download Full-text

Physiological and transcriptome analysis reveal molecular mechanism in Salvia miltiorrhiza leaves of near-isogenic male fertile lines and male sterile lines

BMC Genomics ◽

10.1186/s12864-019-6173-4 ◽

2019 ◽

Vol 20 (1) ◽

Cited By ~ 1

Author(s):

Ruihong Wang ◽

Han Jiang ◽

Ziyun Zhou ◽

Hongbo Guo ◽

Juane Dong

Keyword(s):

Chlorophyll Fluorescence ◽

Plant Growth ◽

Male Sterility ◽

Leaf Gas Exchange ◽

Salvia Miltiorrhiza ◽

Underlying Mechanism ◽

Metabolic Process ◽

Male Sterile ◽

Physiological Analysis

Abstract Background Our previous study finds that male sterility in Salvia miltiorrhiza could result in stunted growth and reduced biomass, but their molecular mechanisms have not yet been revealed. In this article, we investigate the underlying mechanism of male sterility and its impact on plant growth and metabolic yield by using physiological analysis and mRNA sequencing (RNA-Seq). Results In this study, transcriptomic and physiological analysis were performed to identify the mechanism of male sterility in mutants and its impact on plant growth and metabolic yield. Through Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, it is found that the pathways are mainly enriched in processes including organ development, primary metabolic process and secondary metabolic process. Physiological analysis show that the chloroplast structure of male sterile mutants of S. miltiorrhiza is abnormally developed, which could result in decrease in leaf gas exchange (A, E and gs), chlorophyll fluorescence (Fv, Fm and Fv/Fm), and the chlorophyll content. Expression level of 7 differentially expressed genes involved in photosynthesis-related pathways is downregulated in male sterile lines of S. miltiorrhiza, which could explain the corresponding phenotypic changes in chlorophyll fluorescence, chlorophyll content and leaf gas exchange. Transcriptomic analysis establishes the role of disproportionating enzyme 1 (DPE1) as catalyzing the degradation of starch, and the role of sucrose synthase 3 (SUS3) and cytosolic invertase 2 (CINV2) as catalyzing the degradation of sucrose in the S. miltiorrhiza mutants. The results also confirm that phenylalanine ammonialyase (PAL) is involved in the biosynthesis of rosmarinic acid and salvianolic acid B, and flavone synthase (FLS) is an important enzyme catalyzing steps of flavonoid biosynthesis. Conclusions Our results from the physiological and transcriptome analysis reveal underlying mechanism of plant growth and metabolic yield in male sterile mutants, and provide insight into the crop yield of S. miltiorrhiza.

Download Full-text

Physiological and transcriptome analysis reveal molecular mechanism in Salvia miltiorrhiza leaves of near-isogenic male fertile lines and male sterile lines

10.21203/rs.2.9785/v1 ◽

2019 ◽

Author(s):

Ruihong Wang ◽

Hongbo Guo ◽

Juane Dong

Keyword(s):

Plant Growth ◽

Crop Production ◽

Molecular Mechanisms ◽

Leaf Gas Exchange ◽

Salvia Miltiorrhiza ◽

Underlying Mechanism ◽

Metabolic Process ◽

Primary Metabolism ◽

Male Sterile ◽

Physiological Analysis

Abstract Background: Our previous study found that male sterility in Salvia miltiorrhiza could result in stunted growth, decrease biomass, inhibit primary metabolism, and promote secondary metabolism, but their molecular mechanisms have not yet been elucidated. In this article, we investigated the underlying mechanism of plant growth and metabolism by using physiological analysis and mRNA sequencing (RNA-Seq). Results: In this study, transcriptomic and physiological analyses were performed to identify the effect on plant growth and metabolic production in male sterile mutants. Through GO and KEGG analysis it was found that the pathways were mainly enriched in processes including organ development, primary metabolic process and secondary metabolic process. Physiological analyses showed that the chloroplast structure of male sterile mutants of Salvia miltiorrhiza was abnormally developed, which could result in decrease in leaf gas exchange (A, E and gs), chlorophyll fluorescence (Fv, Fm and Fv/Fm), and the chlorophyll content. Transcriptomic analyses indicated that disproportionating enzyme 1 (DPE1) catalyzed the degradation of starch, while sucrose synthase 3 (SUS3) and cytosolic invertase 2 (CINV2) catalyzed the degradation of sucrose in S. miltiorrhiza. The results suggested that phenylalanine ammonialyase (PAL) played an important role in the biosynthesis of rosmarinic acid and salvianolic acid B, and flavone synthase (FLS) was an important enzyme catalyzing steps of flavonoid biosynthesis. High expression level of these enzyme genes in male sterile mutants resulted in high content of secondary metabolites. Conclusions: Our results from the physiological and transcriptome analyses reveal underlying mechanism of plant growth and metabolism in male sterile mutants, and provide insight into the crop production of S. miltiorrhiza.

Download Full-text

Linking exogenous foliar application of glycine betaine and stomatal characteristics with salinity stress tolerance in cotton (Gossypium hirsutum L.) seedlings

BMC Plant Biology ◽

10.1186/s12870-021-02892-z ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Abdoul Kader Mounkaila Hamani ◽

Shuang Li ◽

Jinsai Chen ◽

Abubakar Sunusi Amin ◽

Guangshuai Wang ◽

...

Keyword(s):

Salt Stress ◽

Chlorophyll Fluorescence ◽

Gas Exchange ◽

Plant Growth ◽

Gossypium Hirsutum ◽

Glycine Betaine ◽

Leaf Gas Exchange ◽

Nacl Stress ◽

Stomatal Characteristics ◽

Fluorescence Characteristics

Abstract Background Glycine betaine (GB) plays a crucial role in plants responding to abiotic stresses. Studying the physiological response of cotton seedlings to exogenous GB under salt stress provides a reference for the application of GB to improve the resistance of cotton seedlings under salt stress. The purpose of this research is to examine the impacts of foliar-applied GB on leaf stomatal structure and characteristics, gas exchange and chlorophyll fluorescence characteristics and plant growth indicators of Gossypium hirsutum L. under NaCl stress conditions. Results Under the salinity of 150 mM, the four concentrations of GB are 0, 2.5, 5, and 7.5 mM, and the control (CK) was GB-untreated non-saline. Salt stress negatively affected leaf stomata as well as gas exchange and chlorophyll fluorescence and decreased plant growth parameters of cotton seedlings. The treatment with 5 mM GB significantly increased the evolution of photosynthetic rate (Pn), transpiration rate (Tr), intracellular CO2 concentration (Ci) and stomatal conductance (gs) compared to the GB-untreated saline treatment. The Exogenous foliar-applied GB has sustainably decreased the carboxylation efficiency (Pn/Ci) and water use efficiency (WUE). The concentration of 5 mM GB leads to a significant improvement of leaf stomatal characteristics. The leaf gas exchange attributes correlated positively with stomatal density (SD), stomatal length (SL) and stomatal with (SW). Conclusion The overall results suggested that exogenous foliar supplementation with GB can effectively alleviate the damage of salt stress to cotton seedlings. The effect of applying 5 mM GB could be an optional choice for protecting cotton seedlings from NaCl stress through promoting the stomatal functions, photosynthetic activities and growth characteristics.

Download Full-text

Leaf gas exchange, chlorophyll fluorescence, non-structural carbohydrate content and growth responses of Distylium chinense during complete submergence and subaerial re-emergence

Aquatic Botany ◽

10.1016/j.aquabot.2015.04.003 ◽

2015 ◽

Vol 124 ◽

pp. 70-77 ◽

Cited By ~ 13

Author(s):

Zebin Liu ◽

Ruimei Cheng ◽

Wenfa Xiao ◽

Quanshui Guo ◽

Yanhui Wang ◽

...

Keyword(s):

Chlorophyll Fluorescence ◽

Gas Exchange ◽

Leaf Gas Exchange ◽

Carbohydrate Content ◽

Growth Responses ◽

Structural Carbohydrate

Download Full-text

The oxygenated products of cryptotanshinone by biotransformation with Cunninghamella elegans exerting anti-neuroinflammatory effects by inhibiting TLR 4-mediated MAPK signaling pathway

10.21203/rs.3.rs-19324/v1 ◽

2020 ◽

Author(s):

Jing-Shuai Wu ◽

Qin-Yu Meng ◽

Xiao-Hui Shi ◽

Zhen-Kun Zhang ◽

Hua-Shi Guan ◽

...

Keyword(s):

Signaling Pathway ◽

Transcriptome Analysis ◽

Salvia Miltiorrhiza ◽

Underlying Mechanism ◽

Mapk Signaling ◽

Mapk Signaling Pathway ◽

Mitogen Activated Protein Kinase ◽

Cunninghamella Elegans ◽

Chinese Medicinal Herb ◽

Oxygenated Products

Abstract Background: Neuroinflammatory processes are critical in the development and progression of Alzheimer's disease (AD). The potent anti-neuroinflammatory inhibitors are expected as the candidates to treat AD. Cryptotanshinone (1), a major bioactive constituent in the traditional Chinese medicinal herb Dan-Shen Salvia miltiorrhiza Bunge, has been reported to possess remarkable pharmacological activities, especially anti-oxidation and anti-inflammation. Methods: Cryptotanshinone (1) was biotransformed with the fungus Cunninghamella elegans AS3.2028 to improve its bioactivities and physicochemical properties. The structures of transformed products were elucidated by comprehensive spectroscopic analysis including HRESIMS, NMR and ECD data. Their anti-neuroinflammatory activities were assessed by ELISA, transcriptome analysis, western blot, and immunofluorescence methods. Results: Three oxygenated products (2–4) at C-3 of cryptotanshinone (1) were obtained, among them 2 was a new compound. All of the biotransformed products (2–4) were found to inhibit significantly lipopolysaccharide-induced nitric oxide production in BV2 microglia cells with the IC50 values of 0.16‒1.16 μM, approximately 2‒20 folds stronger than the substrate (1). These biotransformed products also displayed remarkably improved inhibitory effects on the production of inflammatory cytokines (IL-1β, IL-6, TNF-α, COX-2 and iNOS) in BV-2 cells via targeting TLR4 compared to substrate (1). The underlying mechanism of 2 was elucidated by comparative transcriptome analysis, which suggested that it reduced neuroinflammatory mainly through mitogen-activated protein kinase (MAPK) signaling pathway. Western blotting results revealed that 2 downregulated LPS-induced phosphorylation of JNK, ERK, and p38 in MAPK signaling pathway. Conclusion: The biotransformed products of cryptotanshinone exhibit potent anti-neuroinflammatory activities. These findings provide a basal material for the discovery of candidates in treating AD.

Download Full-text

Variations in leaf gas exchange, chlorophyll fluorescence and membrane potential of Medicago sativa root cortex cells exposed to increased salinity: The role of the antioxidant potential in salt tolerance

Archives of Biological Sciences ◽

10.2298/abs171019001f ◽

2018 ◽

Vol 70 (3) ◽

pp. 413-423 ◽

Cited By ~ 2

Author(s):

Mohamed Farissi ◽

Mohammed Mouradi ◽

Omar Farssi ◽

Abdelaziz Bouizgaren ◽

Cherki Ghoulam

Keyword(s):

Antioxidant Enzymes ◽

Salt Stress ◽

Chlorophyll Fluorescence ◽

Membrane Potential ◽

Salt Tolerance ◽

Gas Exchange ◽

Medicago Sativa ◽

Leaf Gas Exchange ◽

Root Cortex ◽

Root Cortex Cells

Salinity is one of the most serious agricultural problems that adversely affects growth and productivity of pasture crops such as alfalfa. In this study, the effects of salinity on some ecophysiological and biochemical criteria associated with salt tolerance were assessed in two Moroccan alfalfa (Medicago sativa L.) populations, Taf 1 and Tata. The experiment was conducted in a hydro-aeroponic system containing nutrient solutions, with the addition of NaCl at concentrations of 100 and 200 mM. The salt stress was applied for a month. Several traits in relation to salt tolerance, such as plant dry biomass, relative water content, leaf gas exchange, chlorophyll fluorescence, nutrient uptake, lipid peroxidation and antioxidant enzymes, were analyzed at the end of the experiment. The membrane potential was measured in root cortex cells of plants grown with or without NaCl treatment during a week. The results indicated that under salt stress, plant growth and all of the studied physiological and biochemical traits were significantly decreased, except for malondialdehyde and H2O2 contents, which were found to be increased under salt stress. Depolarization of membrane root cortex cells with the increase in external NaCl concentration was noted, irrespective of the growth conditions. The Tata population was more tolerant to high salinity (200 mM NaCl) and its tolerance was associated with the ability of plants to maintain adequate levels of the studied parameters and their ability to overcome oxidative stress by the induction of antioxidant enzymes, such as guaiacol peroxidase, catalase and superoxide dismutase.

Download Full-text

Changes in Leaf Structural and Functional Characteristics when Changing Planting Density at Different Growth Stages Alters Cotton Lint Yield under a New Planting Model

Agronomy ◽

10.3390/agronomy9120859 ◽

2019 ◽

Vol 9 (12) ◽

pp. 859 ◽

Cited By ~ 2

Author(s):

Aziz Khan ◽

Jie Zheng ◽

Daniel Kean Yuen Tan ◽

Ahmad Khan ◽

Kashif Akhtar ◽

...

Keyword(s):

Chlorophyll Fluorescence ◽

Quantum Yield ◽

Gas Exchange ◽

Stomatal Density ◽

Leaf Gas Exchange ◽

High Density ◽

Lint Yield ◽

Planting Density ◽

Cotton Lint ◽

Cotton Lint Yield

Manipulation of planting density and choice of variety are effective management components in any cropping system that aims to enhance the balance between environmental resource availability and crop requirements. One-time fertilization at first flower with a medium plant stand under late sowing has not yet been attempted. To fill this knowledge gap, changes in leaf structural (stomatal density, stomatal length, stomata width, stomatal pore perimeter, and leaf thickness), leaf gas exchange, and chlorophyll fluorescence attributes of different cotton varieties were made in order to change the planting densities to improve lint yield under a new planting model. A two-year field evaluation was carried out on cotton varieties—V1 (Zhongmian-16) and V2 (J-4B)—to examine the effect of changing the planting density (D1, low, 3 × 104; D2, moderate, 6 × 104; and D3, dense, 9 × 104) on cotton lint yield, leaf structure, chlorophyll fluorescence, and leaf gas exchange attribute responses. Across these varieties, J-4B had higher lint yield compared with Zhongmian-16 in both years. Plants at high density had depressed leaf structural traits, net photosynthetic rate, stomatal conductance, intercellular CO2 uptake, quenching (qP), actual quantum yield of photosystem II (ΦPSII), and maximum quantum yield of PSII (Fv/Fm) in both years. Crops at moderate density had improved leaf gas exchange traits, stomatal density, number of stomata, pore perimeter, length, and width, as well as increased qP, ΦPSII, and Fv/Fm compared with low- and high-density plants. Improvement in leaf structural and functional traits contributed to 15.9%–10.7% and 12.3%–10.5% more boll m−2, with 20.6%–13.4% and 28.9%–24.1% higher lint yield averaged across both years, respectively, under moderate planting density compared with low and high density. In conclusion, the data underscore the importance of proper agronomic methods for cotton production, and that J-4B and Zhongmian-16 varieties, grown under moderate and lower densities, could be a promising option based on improved lint yield in subtropical regions.

Download Full-text