scholarly journals Metabarcoding Reveals High Diversity of Benthic Foraminifera Driven by Atlantification of Coastal Svalbard

2021 ◽  
Author(s):  
Ngoc-Loi Nguyen ◽  
Joanna Pawłowska ◽  
Inès Barrenechea Angeles ◽  
Marek Zajaczkowski ◽  
Jan Pawłowski
Keyword(s):  
Water Masses ◽  
The Arctic ◽  
Marine Biodiversity ◽  
Reference Database ◽  
Morphological Identification ◽  
Western Coast ◽  
Svalbard Archipelago ◽  
Open Sea ◽  
The Impact ◽  
High Diversity

Abstract Arctic marine biodiversity is undergoing rapid changes due to global warming and modifications of oceanic water masses circulation. These changes have been demonstrated in the case of mega- and macrofauna, but much less is known about their impact on the biodiversity of smaller size organisms, such as foraminifera that represents a main component of meiofauna in the Arctic. Several studies analysed the distribution and diversity of Arctic foraminifera. However, all these studies are based exclusively on the morphological identification of specimens sorted from sediment samples. Here, we present the first assessment of Arctic foraminifera diversity based on metabarcoding of sediment DNA samples collected in fjords and open sea areas in Svalbard Archipelago. We obtained a total of 5,968,786 reads that represented 1,384 ASVs. More than half of the ASVs (51.7%) could not be assigned to any group in the reference database suggesting a high genetic novelty of Svalbard foraminifera. The sieved and unsieved samples resolved comparable communities, sharing 1023 ASVs, comprising over 97% of reads. Our analyses show that the foraminiferal assemblage differs between the localities, with communities distinctly separated between fjord and open sea stations. Each locality was characterized by a specific assemblage, with only a small overlap in the case of open sea areas. Our study demonstrates a clear pattern of the influence of water masses on the structure of foraminiferal communities. The stations situated on the western coast of Svalbard that is strongly influenced by warm and salty Atlantic Water (AW) are characterized by much higher diversity than stations in the northern and eastern part, where the impact of AW is less pronounced. This high diversity and specificity of Svalbard foraminifera associated with water mass distribution indicate that the foraminiferal metabarcoding data can be a very useful tool for inferring present and past environmental conditions in the Arctic.

scholarly journals An 18S V4 rDNA metabarcoding dataset of protist diversity in the Atlantic inflow to the Arctic Ocean, through the year and down to 1000 m depth

2021 ◽  
Author(s):  
Elianne Egge ◽  
Stephanie Elferink ◽  
Daniel Vaulot ◽  
Uwe John ◽  
Gunnar Bratbak ◽  
...  
Keyword(s):  
Arctic Ocean ◽  
High Throughput Sequencing ◽  
Sea Water ◽  
Size Fraction ◽  
The Arctic ◽  
Reference Database ◽  
Rrna Gene ◽  
Svalbard Archipelago ◽  
The Arctic Ocean ◽  
Epipelagic Zone

AbstractArctic marine protist communities have been understudied due to challenging sampling conditions, in particular during winter and in deep waters. The aim of this study was to improve our knowledge on Arctic protist diversity through the year, both in the epipelagic (< 200 m depth) and mesopelagic zones (200-1000 m depth). Sampling campaigns were performed in 2014, during five different months, to capture the various phases of the Arctic primary production: January (winter), March (pre-bloom), May (spring bloom), August (post-bloom) and November (early winter). The cruises were undertaken west and north of the Svalbard archipelago, where warmer Atlantic waters from the West Spitsbergen Current meets cold Arctic waters from the Arctic Ocean. From each cruise, station, and depth, 50 L of sea water were collected and the plankton was size-fractionated by serial filtration into four size fractions between 0.45-200 µm, representing the picoplankton, nanoplankton and microplankton. In addition vertical net hauls were taken from 50 m depth to the surface at selected stations. From the plankton samples DNA was extracted, the V4 region of the 18S rRNA-gene was amplified by PCR with universal eukaryote primers and the amplicons were sequenced by Illumina high-throughput sequencing. Sequences were clustered into Amplicon Sequence Variants (ASVs), representing protist genotypes, with the dada2 pipeline. Taxonomic classification was made against the curated Protist Ribosomal Reference database (PR2). Altogether 6,536 protist ASVs were obtained (including 54 fungal ASVs). Both ASV richness and taxonomic composition were strongly dependent on size-fraction, season, and depth. ASV richness was generally higher in the smaller fractions, and higher in winter and the mesopelagic samples than in samples from the well-lit epipelagic zone during summer. During spring and summer, the phytoplankton groups diatoms, chlorophytes and haptophytes dominated in the epipelagic zone. Parasitic and heterotrophic groups such as Syndiniales and certain dinoflagel-lates dominated in the mesopelagic zone all year, as well as in the epipelagic zone during the winter. The dataset is available at https://doi.org/10.17882/79823, (Egge et al., 2014).

scholarly journals Recent Levels of Technetium-99 in Seawater at the West Coast of Svalbard

2002 ◽  
Vol 2 ◽  
pp. 1507-1513 ◽  
Author(s):  
Sebastian Gerland ◽  
Bjørn Lind ◽  
Mark Dowdall ◽  
Anne Kathrine Kolstad
Keyword(s):  
Additional Data ◽  
Western Coast ◽  
Surface Seawater ◽  
Svalbard Archipelago ◽  
Open Sea ◽  
Nuclear Reprocessing ◽  
Order Of Magnitude ◽  
Arctic Fjord ◽  
European Arctic ◽  
European Nuclear

Seawater from the western coast of Svalbard was sampled in the spring and summer of 2000 to determine levels of technetium-99 (99Tc), a conservative-behaving, manmade radionuclide originating from European nuclear reprocessing plants. This paper deals with the recent levels of this radionuclide in seawater and with the link between an Arctic fjord, Kongsfjorden, and the Western Spitsbergen Current (WSC), investigated using99Tc results. By means of the WSC, the99Tc radionuclides ultimately reach the eastern Fram Strait west of Spitsbergen (the largest island of the Svalbard archipelago). Results from oceanographic modelling and sea ice observations indicate a direct coupling between Kongsfjorden and the area west of it. The findings in connection with new radionuclide results presented in this paper concur with these assumptions. Furthermore they indicate that the inner part of Kongsfjorden is also well linked to the WSC. Surface seawater from the central part of the WSC, sampled during a cruise with RV Polarstern in the summer of 2000, shows a higher level of99Tc than those measured in Kongsfjorden in spring 2000. However, all levels measured in surface water are of the same order of magnitude. Data from sampling of deeper water in the WSC area provide information pertaining to the lateral distribution of99Tc. The results, along with additional data from spring 2001, indicate that Kongsfjorden is suitable for monitoring the levels of99Tc arriving in the European Arctic and that the sheltered setting of this fjord does not necessarily provide protection against pollution from the open sea.

scholarly journals Impact of seasonal variations of ambient seismic noise level on the number of registered earthquakes in the Arctic regions

2019 ◽  
Vol 19 (3) ◽  
pp. 123-128
Author(s):  
ER Morozova ◽  
AP Turova
Keyword(s):  
Seasonal Variations ◽  
The Arctic ◽  
Svalbard Archipelago ◽  
Russian Academy Of Sciences ◽  
Arctic Regions ◽  
European Arctic ◽  
Academy Of Sciences ◽  
International Seismological Centre ◽  
The Impact

Researchers at the Seismological Laboratory of the Institute of Geodynamics and Geology of the Federal Center for Integrated Arctic Research of the Russian Academy of Sciences (FCIARctic) have been engaged in the seismological monitoring of the European Arctic sector since 2011. In this paper, we present a comparative assessment of the earthquakes spatial distribution in this region based on the data from the International Seismological Centre (ISC) and the FCIARctic’s Arkhangelsk Seismic Network (ASN) obtained in 2012–2016. The paper presents the waveforms of earthquakes occurred at the Gakkel Ridge and the Svalbard archipelago processed with the use of a Russian software package WSG (Windows Seismic Grafer) recommended by the Unified Geophysical Service of the Russian Academy of Sciences. A standard 4–8 Hz bandpass filter was used for the processing of regional Arctic earthquakes. The impact of seasonal variations on the quality of earthquakes registration was analysed based on the seismograms recorded by the ASN’s island-based Arctic stations from 2012 to 2014 The same analysis was done for the central broadband sensor SPA0 of the Norwegian NORSAR-owned SPITS group installed at the Svalbard archipelago. A correlation has been established between the number of earthquakes recorded by the ASN’s island Arctic stations and SPA0 station. The number of regional earthquakes, recorded by ASN’s island Arctic stations is is smaller in summer-autumn periods than in winter periods. Forthe SPA0 station, which is part of SPITS group, there is not seasonality in the number of registered earthquakes. Generally, earthquakes are recorded uniformly, exception on January. This might be due to the increased seismic activity in the Svalbard archipelago during that period.

scholarly journals Environmental genomics for benthic monitoring of North Sea oil and gas offshore platforms

2021 ◽  
Vol 4 ◽  
Author(s):  
Jan Pawlowski ◽  
Florian Mauffrey ◽  
Tristan Cordier ◽  
Laure Apothéloz-Perret-Gentil ◽  
Kristina Cermakova ◽  
...  
Keyword(s):  
Oil And Gas ◽  
De Novo ◽  
Ecological Status ◽  
Oil And Gas Industry ◽  
Marine Biodiversity ◽  
Morphological Identification ◽  
Offshore Platforms ◽  
Time Saving ◽  
Environmental Pressures ◽  
The Impact

During the last decade, considerable efforts have been undertaken to achieve a “good ecological status” of European coastal waters and ensuring the development of methodological standards for the evaluation of this status. However, the current routine biomonitoring implicates time-consuming and costly manual sorting and morphological identification of benthic macrofauna. In our study, we tested the performance of environmental DNA metabarcoding targeting microbial communities and meiofauna as an alternative to traditional macrofauna-based monitoring. We focused on environmental impact assessment of offshore oil and gas industry. We used three genetic markers (18S V1V2, 18S V9 and COI) to assess the environmental pressures induced by the platforms. All markers showed patterns of alpha and beta diversity consistent with morphology-based macrofauna analyses, significantly changing along distance gradients from the platforms. The impact of the operational discharges was also detected by the variation of biotic indices values, AMBI index showing the best correlation between morphological and eDNA datasets. Finally, the sediment physicochemical parameters were used to build a local de novo pressure index that served as benchmark to test the potential of a taxonomy-free approach. Our study demonstrates that metabarcoding approach outperforms morphology-based approach and can be used as a cost and time-saving alternative solution to the traditional morphology-based monitoring in order to monitor more efficiently the impact of industrial activities on marine biodiversity.

scholarly journals Cost-Effective Technologies to Study the Arctic Ocean Environment †

Sensors ◽  
2018 ◽  
Vol 18 (7) ◽  
pp. 2257 ◽  
Author(s):  
Viviana Piermattei ◽  
Alice Madonia ◽  
Simone Bonamano ◽  
Riccardo Martellucci ◽  
Gabriele Bruzzone ◽  
...  
Keyword(s):  
Arctic Ocean ◽  
Low Cost ◽  
Marine Ecosystem ◽  
Arctic Region ◽  
Extreme Environment ◽  
The Arctic ◽  
Svalbard Archipelago ◽  
The Arctic Ocean ◽  
The Impact ◽  
The Arctic Region

The Arctic region is known to be severely affected by climate change, with evident alterations in both physical and biological processes. Monitoring the Arctic Ocean ecosystem is key to understanding the impact of natural and human-induced change on the environment. Large data sets are required to monitor the Arctic marine ecosystem and validate high-resolution satellite observations (e.g., Sentinel), which are necessary to feed climatic and biogeochemical forecasting models. However, the Global Observing System needs to complete its geographic coverage, particularly for the harsh, extreme environment of the Arctic Region. In this scenario, autonomous systems are proving to be valuable tools for increasing the resolution of existing data. To this end, a low-cost, miniaturized and flexible probe, ArLoC (Arctic Low-Cost probe), was designed, built and installed on an innovative unmanned marine vehicle, the PROTEUS (Portable RObotic TEchnology for Unmanned Surveys), during a preliminary scientific campaign in the Svalbard Archipelago within the UVASS project. This study outlines the instrumentation used and its design features, its preliminary integration on PROTEUS and its test results.

Methane in frozen and thawed soils of the western sector of Russian Arctic

2021 ◽  
Author(s):  
Nataliia Zadorozhnaia ◽  
Gleb Oblogov ◽  
Alexander Vasiliev ◽  
Irina Streletskaya
Keyword(s):  
Transition Zone ◽  
Active Layer ◽  
River Estuary ◽  
Methane Content ◽  
The Arctic ◽  
Yamal Peninsula ◽  
Western Coast ◽  
Permafrost Degradation ◽  
Pechora River ◽  
The Impact

&lt;p&gt;Many researchers study the Earth's climate change and the impact of the greenhouse effect on this process. The large amount of methane (CH&lt;sub&gt;4&lt;/sub&gt;) is preserved in permafrost. In this regard, scientists recently pay a great attention to the problem of methane emission during the permafrost degradation in the Arctic zone. Until now, the methane content in underground ice, frozen Quaternary sediments has been studied insufficiently. The methane content in the active layer is especially poorly studied.&lt;/p&gt;&lt;p&gt;The authors researched methane content in frozen grounds of the upper permafrost horizon (transition zone) and in thawed sediments of the active layer for different tundra landscapes near the Marre-Sale polar station on the western coast of the Yamal peninsula and for landscapes of the Pechora river estuary area (Russia).&lt;/p&gt;&lt;p&gt;More than 420 samples of gas from sediments in active and transient layer were collected in Marre-Sale and 36 samples in Pechora area. To determine the methane content, the samples were placed in syringes and degassed using the &amp;#8220;head space&amp;#8221; technique. CH&lt;sub&gt;4&lt;/sub&gt; measurements were carried out on a chromatograph with flame ionization detector (FID) Shimadzu GC-2014 (Japan) in the laboratory of Federal State Institution &amp;#8220;VNIIOkeangeologiya&amp;#8221; (Saint-Petersburg, Russia).&lt;/p&gt;&lt;p&gt;Methane content in the frozen and thawed sediments of different dominant landscapes of typical tundra on Yamal peninsula and landscapes of southern tundra on Pechora area is extremely variable. The greatest amount of methane is typical for the most wet landscapes with primarily of silt loam soils. In dry primarily sandy well-drained landscapes, the methane content is low. The highest methane content is measured within the low floodplain of river, water tracks, swampy depressions of polygonal relief, and lake basins landscapes (mean varied from 0.8 to 2.5 ml [CH4] / kg, with a maximum of 9.0 ml [CH4] / kg). For landscapes of the moist surface of typical tundra, the average values of methane content were approximately 0.4 ml [CH4] / kg (with a maximum of 3.4 ml [CH4] / kg). The lowest methane contents in soils were characteristic of the landscapes of well-drained tundra, and sand fields where the average values do not exceed 0.2 ml [CH4] / kg. Mean methane content in soils of Pechora river mouth landscapes varied from 0.05 to 4.5 ml [CH4] / kg, with a maximum of 15.8 ml [CH4] / kg.&lt;/p&gt;&lt;p&gt;Determined that methane contents in the frozen soils of the transition zone is 2 to 5 times higher than in the soils of the active layer. High content of methane in upper layers of permafrost should be considered as a significant source of methane, which can be involved in emission of greenhouse gases into the atmosphere during permafrost degradation.&lt;/p&gt;

scholarly journals An 18S V4 rDNA metabarcoding dataset of protist diversity in the Atlantic inflow to the Arctic Ocean, through the year and down to 1000 m depth

2021 ◽  
Author(s):  
Elianne Egge ◽  
Stephanie Elferink ◽  
Daniel Vaulot ◽  
Uwe John ◽  
Gunnar Bratbak ◽  
...  
Keyword(s):  
Arctic Ocean ◽  
High Throughput Sequencing ◽  
Sea Water ◽  
Size Fraction ◽  
The Arctic ◽  
Reference Database ◽  
Rrna Gene ◽  
Svalbard Archipelago ◽  
The Arctic Ocean ◽  
Epipelagic Zone

Abstract. Arctic marine protist communities have been understudied due to challenging sampling conditions, in particular during winter and in deep waters. The aim of this study was to improve our knowledge on Arctic protist diversity through the year, both in the epipelagic (< 200 m depth) and mesopelagic zones (200–1000 m depth). Sampling campaigns were performed in 2014, during five different months, to capture the various phases of the Arctic primary production: January (winter), March (pre-bloom), May (spring bloom), August (post-bloom) and November (early winter). The cruises were undertaken west and north of the Svalbard archipelago, where warmer Atlantic waters from the West Spitsbergen Current meets cold Arctic waters from the Arctic Ocean. From each cruise, station, and depth, 50 L of sea water were collected and the plankton was size-fractionated by serial filtration into four size fractions between 0.45–200 μm, representing the picoplankton, nanoplankton and microplankton. In addition vertical net hauls were taken from 50 m depth to the surface at selected stations. From the plankton samples DNA was extracted, the V4 region of the 18S rRNA-gene was amplified by PCR with universal eukaryote primers and the amplicons were sequenced by Illumina high-throughput sequencing. Sequences were clustered into Amplicon Sequence Variants (ASVs), representing protist genotypes, with the dada2 pipeline. Taxonomic classification was made against the curated Protist Ribosomal Reference database (PR2). Altogether 6,536 protist ASVs were obtained (including 54 fungal ASVs). Both ASV richness and taxonomic composition were strongly dependent on size-fraction, season, and depth. ASV richness was generally higher in the smaller fractions, and higher in winter and the mesopelagic samples than in samples from the well-lit epipelagic zone during summer. During spring and summer, the phytoplankton groups diatoms, chlorophytes and haptophytes dominated in the epipelagic zone. Parasitic and heterotrophic groups such as Syndiniales and certain dinoflagellates dominated in the mesopelagic zone all year, as well as in the epipelagic zone during the winter. The dataset is available at https://doi.org/10.17882/79823 (Egge et al. 2014).

scholarly journals An 18S V4 rRNA metabarcoding dataset of protist diversity in the Atlantic inflow to the Arctic Ocean, through the year and down to 1000 m depth

2021 ◽  
Vol 13 (10) ◽  
pp. 4913-4928
Author(s):  
Elianne Egge ◽  
Stephanie Elferink ◽  
Daniel Vaulot ◽  
Uwe John ◽  
Gunnar Bratbak ◽  
...  
Keyword(s):  
Arctic Ocean ◽  
High Throughput Sequencing ◽  
18S Rrna Gene ◽  
The Arctic ◽  
Reference Database ◽  
Rrna Gene ◽  
Svalbard Archipelago ◽  
Size Fractions ◽  
The Arctic Ocean ◽  
Epipelagic Zone

Abstract. Arctic marine protist communities have been understudied due to challenging sampling conditions, in particular during winter and in deep waters. The aim of this study was to improve our knowledge on Arctic protist diversity through the year, in both the epipelagic (< 200 m depth) and mesopelagic zones (200–1000 m depth). Sampling campaigns were performed in 2014, during five different months, to capture the various phases of the Arctic primary production: January (winter), March (pre-bloom), May (spring bloom), August (post-bloom), and November (early winter). The cruises were undertaken west and north of the Svalbard archipelago, where warmer Atlantic waters from the West Spitsbergen Current meet cold Arctic waters from the Arctic Ocean. From each cruise, station, and depth, 50 L of seawater was collected, and the plankton was size-fractionated by serial filtration into four size fractions between 0.45–200 µm, representing picoplankton (0.45–3 µm), small and large nanoplankton (3–10 and 10–50 µm, respectively), and microplankton (50–200 µm). In addition, vertical net hauls were taken from 50 m depth to the surface at selected stations. The net hauls were fractionated into the large nanoplankton (10–50 µm) and microplankton (50–200 µm) fractions. From the plankton samples DNA was extracted, the V4 region of the 18S rRNA-gene was amplified by polymerase chain reaction (PCR) with universal eukaryote primers, and the amplicons were sequenced by Illumina high-throughput sequencing. Sequences were clustered into amplicon sequence variants (ASVs), representing protist genotypes, with the dada2 pipeline. Taxonomic classification was made against the curated Protist Ribosomal Reference database (PR2). Altogether, 6536 protist ASVs were obtained (including 54 fungal ASVs). Both ASV richness and taxonomic composition varied between size fractions, seasons, and depths. ASV richness was generally higher in the smaller fractions and higher in winter and the mesopelagic samples than in samples from the well-lit epipelagic zone during summer. During spring and summer, the phytoplankton groups diatoms, chlorophytes, and haptophytes dominated in terms of relative read abundance in the epipelagic zone. Parasitic and heterotrophic groups such as Syndiniales and certain dinoflagellates dominated in the mesopelagic zone all year, as well as in the epipelagic zone during the winter. The dataset is available at https://doi.org/10.17882/79823 (Egge et al., 2014).

A preliminary archaeological survey of Camp Wellman at Virgohamn, Danskøya, Svalbard

Polar Record ◽  
1994 ◽  
Vol 30 (175) ◽  
pp. 265-276 ◽  
Author(s):  
P.J. Capelotti
Keyword(s):  
North Pole ◽  
The Arctic ◽  
Archaeological Survey ◽  
Svalbard Archipelago ◽  
The North ◽  
Base Camp ◽  
The Impact ◽  
First Time

ABSTRACTBetween 1894 and 1909, Walter Wellman (1858–1934), a Chicago-based journalist and explorer, organized and led five expeditions to reach the North Pole. Wellman launched his most ambitious undertaking in 1906, constructing an extensive base camp complete with an airship hangar on the shoreline of Virgohamn, a small harbor on Danskøya in the Svalbard archipelago. In 1907 and 1909, Wellman's two flights in the dirigible airship America marked the first time a motorized airship had flown in the Arctic. In the summer of 1993, the author surveyed and mapped the extent of the remains of Wellman's camp to discover and document any surviving wreckage of America, and to observe the impact of tourist traffic on the site. This paper describes the 1993 survey of Wellman's base camp at Virgohamn and offers suggestions for its preservation.

Consideration of Geopolitical Challenges within the Analysis of Geoenvironmental Risks for Oil and Gas Development of the Russian Arctic

2019 ◽  
Vol 16 (6) ◽  
pp. 50-59
Author(s):  
O. P. Trubitsina ◽  
V. N. Bashkin
Keyword(s):  
Oil And Gas ◽  
Oil And Gas Industry ◽  
Optimal Operation ◽  
The Arctic ◽  
Stage Model ◽  
Oil And Gas Development ◽  
Gas Industry ◽  
The Impact ◽  
Further Development ◽  
Geopolitical Risks

The article is devoted to the consideration of geopolitical challenges for the analysis of geoenvironmental risks (GERs) in the hydrocarbon development of the Arctic territory. Geopolitical risks (GPRs), like GERs, can be transformed into opposite external environment factors of oil and gas industry facilities in the form of additional opportunities or threats, which the authors identify in detail for each type of risk. This is necessary for further development of methodological base of expert methods for GER management in the context of the implementational proposed two-stage model of the GER analysis taking to account GPR for the improvement of effectiveness making decisions to ensure optimal operation of the facility oil and gas industry and minimize the impact on the environment in the geopolitical conditions of the Arctic.The authors declare no conflict of interest

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