scholarly journals Bacterial communities in the polluted area of pyrite tailings: From the upstream, pollutant source, and to the downstream

2021 ◽  
Author(s):  
Wenkai Li ◽  
Yun Han ◽  
Hongwei Lei ◽  
Tianlong Zheng ◽  
Yiming Lian ◽  
...  
Keyword(s):  
Bacterial Communities ◽  
Physicochemical Characteristics ◽  
Polluted Area ◽  
Illumina Hiseq ◽  
Water And Sediment ◽  
Illumina Hiseq Sequencing ◽  
Strong Acidity ◽  
Treatment Technologies ◽  
Pollutant Source ◽  
Pyrite Tailings

Abstract Pyrite tailings can cause serious pollution to the surface water as the strong acidity, high iron and sulfate concentration in the leachate. The bacterial communities of pyrite tailings polluted area were still unclear which could restrict the recognition of the pyrite tailings pollution effect and further impede the development of microbial or ecology treatment technologies. In this study, the bacterial communities in the polluted area of pyrite tailings, from the upstream, pollutant source, and to the downstream, were analyzed with Illumina HiSeq sequencing. Results showed that Acinetobacter and Flavobacterium were abundant in the water and sediment of upstream and downstream while Bacteroides, Lactobacillus, and Akkermansia were abundant in the pollutant source. Sulfur-metabolizing or iron-metabolizing bacteria extensively existed in the polluted area in which Acidiferrobacter, Ferrithrix, and Desulfovibrio played crucial roles on the whole communities. Sulfur-metabolizing bacteria (e.g. Thiomonas, Sulfurospirillum, and Desulfobulbus) and iron-metabolizing bacteria (e.g. Ferrimicrobium, Ferrithrix, and Ferrovum) were introduced to the river polluted by pyrite tailings. Pyrite tailings can remarkably change the physicochemical characteristics and bacterial communities of river water and sediment.

Evolution of bacterial consortia in an integrated tannery wastewater treatment process

RSC Advances ◽  
2016 ◽  
Vol 6 (90) ◽  
pp. 87380-87388 ◽  
Author(s):  
Hebin Liang ◽  
Dongdong Ye ◽  
Pan Li ◽  
Tingting Su ◽  
Jiegen Wu ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Bacterial Communities ◽  
Treatment Process ◽  
Sewage Treatment ◽  
Physicochemical Characteristics ◽  
Tannery Wastewater ◽  
Illumina Hiseq ◽  
Wastewater Treatment Process ◽  
Bacterial Consortia

PCR-DGGE and Illumina HiSeq revealed the composition of bacterial communities in tannery sewage treatment and their linkages with the physicochemical characteristics of wastewater.

scholarly journals Bacterial Abundance and Physicochemical Characteristics of Water and Sediment Associated with Hydroelectric Dam on the Lancang River China

2019 ◽  
Vol 16 (11) ◽  
pp. 2031 ◽  
Author(s):  
Xia Luo ◽  
Xinyi Xiang ◽  
Guoyi Huang ◽  
Xiaorui Song ◽  
Peijia Wang ◽  
...  
Keyword(s):  
Bacterial Abundance ◽  
Water Discharge ◽  
Operating Regime ◽  
Physicochemical Characteristics ◽  
Total Dissolved Solids ◽  
Epifluorescence Microscopy ◽  
Dissolved Solids ◽  
Lancang River ◽  
Water And Sediment ◽  
Dam Site

Research on bacterial abundance in water column and sediment of dammed rivers remain poorly understood, despite their importance to biogeochemical processes, benthic ecology, and bioremediation. The present study investigates the water and sediment bacteria by epifluorescence microscopy in the reservoir (above the dam site), as well as in the downstream river stretches (below-dam site) at the middle reach of Lancang River during the wet, the normal and the dry seasons. The results demonstrated that the reservoir operating regime (water discharge variations) and strong precipitation promoted significant differences in the conditions of the river below the dam, especially for the concentration of dissolved oxygen, redox potential, electric conductivity, turbidity, and total dissolved solids in water and concentration of microbial activity in sediment. The seasonal variations were also key factors influencing water quality at the below-dam sampling sites. Nutrients concentration did not induce a significant response in bacterial abundance when inorganic nutrients were sufficient. Bacterial density in sediment was regulated by hydropower-related discharge, particle size, and type of sediments, while bacterial abundances in water were strongly linked with the physicochemical characteristics of the water, such as total dissolved solids and conductivity.

scholarly journals Pollination Drop Proteome and Reproductive Organ Transcriptome Comparison in Gnetum Reveals Entomophilous Adaptation

Genes ◽  
2019 ◽  
Vol 10 (10) ◽  
pp. 800 ◽  
Author(s):  
Chen Hou ◽  
Richard M. K. Saunders ◽  
Nan Deng ◽  
Tao Wan ◽  
Yingjuan Su
Keyword(s):  
Reproductive Organs ◽  
Reproductive Organ ◽  
Differentially Expressed ◽  
Label Free ◽  
Pollination Syndromes ◽  
Illumina Hiseq ◽  
Reproductive Structures ◽  
Illumina Hiseq Sequencing ◽  
Pollination Drop ◽  
Transcriptome Comparison

Gnetum possesses morphologically bisexual but functionally unisexual reproductive structures that exude sugary pollination drops to attract insects. Previous studies have revealed that the arborescent species (G. gnemon L.) and the lianoid species (G. luofuense C.Y.Cheng) possess different pollination syndromes. This study compared the proteome in the pollination drops of these two species using label-free quantitative techniques. The transcriptomes of fertile reproductive units (FRUs) and sterile reproductive units (SRUs) for each species were furthermore compared using Illumina Hiseq sequencing, and integrated proteomic and transcriptomic analyses were subsequently performed. Our results show that the differentially expressed proteins between FRUs and SRUs were involved in carbohydrate metabolism, the biosynthesis of amino acids and ovule defense. In addition, the differentially expressed genes between the FRUs and SRUs (e.g., MADS-box genes) were engaged in reproductive development and the formation of pollination drops. The integrated protein-transcript analyses revealed that FRUs and their exudates were relatively conservative while the SRUs and their exudates were more diverse, probably functioning as pollinator attractants. The evolution of reproductive organs appears to be synchronized with changes in the pollination drop proteome of Gnetum, suggesting that insect-pollinated adaptations are not restricted to angiosperms but also occur in gymnosperms.

scholarly journals The Keggin Structure: An Important Factor in Governing NH3–SCR Activity Over the V2O5–MoO3/TiO2 Catalyst

2018 ◽  
Vol 148 (4) ◽  
pp. 1228-1235 ◽  
Author(s):  
Rui Wu ◽  
Ningqiang Zhang ◽  
Xiaojun Liu ◽  
Lingcong Li ◽  
Liyun Song ◽  
...  
Keyword(s):  
Low Temperature ◽  
Catalytic Reduction ◽  
Physicochemical Characteristics ◽  
Acid Sites ◽  
Keggin Structure ◽  
Lewis Acid Sites ◽  
Nh3 Scr ◽  
Strong Acidity ◽  
Ft Ir ◽  
Brønsted And Lewis Acid

Abstract Heteropolyacids and their salts have been effectively used in selective catalytic reduction because of the Keggin structure and extraordinarily strong acidity. Catalysts with and without the Keggin structure were synthesized to further investigate the effects of heteropolyoxometallate on low temperature NH3–SCR. XRD, BET, Raman, H2–TPR, NH3–TPD, FT-IR, and SO2–TPD techniques were used to characterize the physicochemical characteristics of the catalysts. Results indicate that catalysts with the Keggin structure had more surface Brönsted and Lewis acid sites, and these catalysts had significantly improved performances in the SCR reaction and in SO2 poisoning resistance. Graphical Abstract

Integrated biogeography and assembly process of planktonic and sedimentary bacterial communities in the Yangtze River

2020 ◽  
Author(s):  
Tang Liu ◽  
Jiawen Wang ◽  
Shufeng Liu ◽  
Qian Chen ◽  
Chunmiao Zheng ◽  
...  
Keyword(s):  
Bacterial Communities ◽  
Yangtze River ◽  
Selection Process ◽  
Anthropogenic Impacts ◽  
Spatiotemporal Pattern ◽  
Assembly Process ◽  
The Yangtze River ◽  
Species Sorting ◽  
Biogeographic Patterns ◽  
Water And Sediment

<p>Bacterial communities are essential to the biogeochemical cycle in riverine ecosystems. However, the integrated biogeography and assembly process of planktonic and sedimentary bacterial communities in large rivers is still poorly understood. Here, the study provided the spatiotemporal pattern of bacterial communities in the Yangtze River of 4300 km continuum, which is the largest river in Asia. We found that the taxa in sediments are the main contributors to the bacterial diversity of the river ecosystem since sediments sub-group took 98.8% of the total 38, 904 Operational Taxonomic Units (OTUs) observed in 280 samples. Seasonal differences in bacterial communities were statistically significant in water, whereas bacterial communities in both water and sediment were geographically clustered according to five types of landforms: mountain, foothill, basin, foothill-mountain, and plain. Interestingly, the presence of two huge dams resulted in a drastic fall of bacterial taxa in sediment immediately downstream due to severe riverbed scouring. The integrity of the biogeography was satisfactorily interpreted by the combination of neutral and species sorting perspectives in meta-community theory for bacterial communities in flowing water and sediment. Although deterministic process had dominant influence on assembly processes in water and sediment communities, homogeneous selection was the main contributor in water, while combination of homogeneous selection and variable selection contributed selection process in sediment. In addition, homogenizing dispersal played more important role in community assembly process in sediment than water. Our study fills a gap in understanding of biogeography and assembly process of bacterial communities in one of the world’s largest river and highlights the importance of both planktonic and sedimentary communities to the integrity of bacterial biogeographic patterns in a river subject to varying natural and anthropogenic impacts.</p>

scholarly journals Physicochemical parameters and microbial loads of marine cage farm environment at Polem, Goa

2017 ◽  
Vol 64 (4) ◽  
Author(s):  
Mamatha H. Hazari ◽  
J. L. Rathod
Keyword(s):  
Significant Variation ◽  
Physicochemical Parameters ◽  
Inorganic Phosphate ◽  
Heterotrophic Bacteria ◽  
Sediment Quality ◽  
Bacterial Count ◽  
Physicochemical Characteristics ◽  
Optimum Range ◽  
Water And Sediment ◽  
Water And Sediment Quality

Present study was undertaken at the marine cage farm of Polem Village, Goa. Water and sediment samples were analysed for evaluation of the physicochemical characteristics and total cultivable heterotrophic bacteria. No significant variation was observed in the physicochemical parameters of water except for inorganic phosphate (p<0.05). Total cultivable bacterial count ranged from 2.06 to 19.27 x 104cfu g-1 and from 1.14 to 8.41 x 104 cfu g-1, while total presumptive vibrio count ranged from 0.15 to 1.57 x 104 g-1 and 0.09 to 0.78 x 10-4 g-1. Results of the present investigation revealed that all the parameters studied were within the optimum range and cage farming with limited number of cages does not have major impacts on the water and sediment quality.

scholarly journals Determination of primary microRNA processing in clinical samples by targeted pri-miR-sequencing

2020 ◽  
Author(s):  
Thomas Conrad ◽  
Evgenia Ntini ◽  
Benjamin Lang ◽  
Luca Cozzuto ◽  
Jesper B Andersen ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
High Sensitivity ◽  
Normal Liver ◽  
Microrna Expression ◽  
Clinical Samples ◽  
Processing Efficiency ◽  
Illumina Hiseq ◽  
Illumina Hiseq Sequencing ◽  
Input Material ◽  
Microrna Processing

AbstractMicroRNA expression is important for gene regulation and deregulated microRNA expression is often observed in disease such as cancer. The processing of primary microRNA transcripts is an important regulatory step in microRNA biogenesis. Due to low expression level and association with chromatin primary microRNAs are challenging to study in clinical samples where input material is limited.Here, we present a high-sensitivity targeted method to determine processing efficiency of several hundred primary microRNAs from total RNA using as little as 500 thousand Illumina HiSeq sequencing reads. We validate the method using RNA from HeLa cells and show the applicability to clinical samples by analyzing RNA from normal liver and hepatocellular carcinoma.We identify 24 primary microRNAs with significant changes in processing efficiency from normal liver to hepatocellular carcinoma, among those the highly expressed miRNA-122 and miRNA-21, demonstrating that differential processing of primary microRNAs is occurring and could be involved in disease. With our method presented here we provide means to study pri-miRNA processing in disease from clinical samples.

The mitochondrial genome and phylogenetic analysis of the tick Dermacentor everestianus Hirst, 1926 (Acari: Ixodidae)

2018 ◽  
Vol 23 (7) ◽  
pp. 1313 ◽  
Author(s):  
Zhijun Yu ◽  
Shiqi Zhang ◽  
Tianhong Wang ◽  
Xiaolong Yang ◽  
Hui Wang ◽  
...  
Keyword(s):  
Tandem Repeats ◽  
Gc Content ◽  
Ixodid Tick ◽  
Tibet Plateau ◽  
Illumina Hiseq ◽  
Protein Coding ◽  
Illumina Hiseq Sequencing ◽  
Dermacentor Everestianus ◽  
Mt Genome ◽  
Rna Genes

The tick Dermacentor everestianus is widely distributed in Qinghai-Tibet Plateau of China and can transmit many zoonotic pathogens. In the current study, the complete mitochondrial (mt) genome of D. everestianus was sequenced through Illumina HiSeq sequencing. The mt genome is 15,191 bp in length which contains 37 genes, including 13 protein-coding genes, two ribosomal RNA genes and 22 transfer RNA genes. The overall GC content reached 21.20%, whereas the GC content in the gene region was 21.40%, and in the intergenetic region was 20.50%. Two control regions were sequenced from mt genome of D. everestianus, one located between tRNA-Cys and tRNA-Leu, and the other one was found between rrnS and tRNA-Ile. Two tandem repeats were found between tRNA-Glu and nad1. A phylogenetic tree was constructed based on the complete mitogenome of D. everestianus and 32 other ixodid tick mitogenomes to assess their phylogenetic relationships. D. everestianus is phylogenetically clustered with the tick D. silvarum and D. nuttalli. This is the first complete mt genome of D. everestianus, which provides a useful reference for future studies on systematics and population genetics of this tick species.

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