scholarly journals Validating Field Regeneration Capacity for Selected Accessions of Gossypium hirsutum Using Callus Induction and Regeneration Capacity

Author(s):  
Sani Muhammad Tajo ◽  
Zhao Pan ◽  
Salisu Bello Sadau ◽  
Shahid Iqbal ◽  
KM Yusuf ◽  
...  

Abstract Gossypium hirsutm undergoes a rapid clonal propagation to regenerate a mature plant through tissue culture. In this research, cotton leaf regeneration level for 21 accessions in the field (new leaves) was observed after the first harvest, and a comparison between field regeneration level and callus induction with its regeneration capacity (new shoots and roots) for the same 21 accessions was carried out. During the flowering stage of Gossypium hirsutum, biochemical (Proline), physiological (chlorophyll and carotenoid content) analysis was carried out. Phenotypic observations (plant height, leaf area, fresh leaf weight, dry leaf weight, flower and boll number) were also carried out on 21 accessions for each accession. Callus induction and regeneration capacity of roots and shoots for hypocotyl, cotyledons and shoot tip tissues was used to validate field regeneration capacity through analysis of variance. ZS061, LuMian378, JiMian863, and ZS065 have highest drought tolerance while ZhongMianSuo24, LiaoYangDuoMaoMian, and BeiZheGongSheMian have the lowest tolerance to drought stress. Accessions with both field and callus regeneration capacity were identified.

2021 ◽  
pp. 1-6
Author(s):  
Chunjia Li ◽  
Xujuan Li ◽  
Xiuqin Lin ◽  
Wei Qin ◽  
Xin Lu ◽  
...  

Abstract Embryogenic callus induction and regeneration are useful in many aspects of plant biotechnology, especially in the functional characterization of economically important genes. However, in sugarcane, callus induction and regeneration vary across genotypes. Saccharum spontaneum is an important wild germplasm that confers disease resistance and stress tolerance to modern sugarcane cultivars, and its genome has been completely sequenced. The aim of this study was to investigate the effect of genetic variations on embryogenic callus induction and regeneration in S. spontaneum and to screen genotypes having high tissue culture susceptibility. The study was performed using nine genotypes of S. spontaneum and the following five parameters were assessed to determine the response of genotypes to embryogenic callus induction and regeneration: callus induction, embryogenic callus ratio, embryogenic callus induction, embryonic callus regeneration and regeneration capacity. All the genotypes varied significantly (P < 0.01) in all the parameters, except for embryonic callus regeneration, which was high (>80%) for all the genotypes. High broad-sense heritability (86.1–96.8%) indicated that genetic differences are the major source of genotypic variations. Callus induction was found to be strongly positively correlated with embryogenic callus induction (r = 0.890, P < 0.01) and regeneration capacity (r = 0.881, P < 0.01). Among the nine tested genotypes, VN2 was found to be the most responsive to tissue culture and could therefore be used to characterize functional genes in S. spontaneum. We also suggested an approach with potential applications in facilitating the rapid identification of sugarcane genotypes susceptible to tissue culture.


Author(s):  
Mahmut Çamlıca ◽  
Gülsüm Yaldız ◽  
Ferit Özen ◽  
Abdurrahman Başol ◽  
Halit Aşkın

Sage and mountain sage belong to Lamiaceae family which have commercial importance from medicinal and aromatic plants in Turkey. The aim of this study was to evaluate the effects of different doses of selenium in salinity conditions on the morphological characteristics of sage (Salvia officinalis L.) and mountain tea (Sideritis sp.). Four different doses of selenium (5, 10, 20, 40 mg/l) were applied in without salinity (0) and salinity (250 mM NaCl/l) medium. The experiment was carried out in the split plot design with three replications in the climate chamber room of the department of field crops. When the examined properties were evaluated; plant height changed between 15.56-23.85 cm, number of branches 10.50-12.78 number/plant, number of leaves 52.78-92.00 number/plant, fresh leaf weight 2.48-7.51 g/plant, dry leaf weight 0.48-3.32 g/plant, fresh root weight 1.52-7.16 g/plant, dry root weight 0.19-1.24 g/plant, root length 26.18-36.07 cm, fresh shoot weight of 1.13-7.15 g/plant and dry shoot weight 0.13-0.38 g/plant. In mountain tea, the properties were determined for plant height as 3.26-5.93 cm, for number of branches as 2.50-6.33 number/plant, for number of leaves as 28.22-91.14 number/plant, for fresh and dry leaf weights as 2.42-11,03 and 0.45-1.91 g/plant, for fresh and dry root weights as 0.71-3.97 and 0.18-0.74 g/plant, for root length as 14.78-33.26 cm, for fresh and dry shoot weights as 0.29-2.28 and 0.12-0.41 g/plant were determined. As a result of this study, 5 mg/l selenium application in salinity conditions in both plants has reached high values in terms of fresh leaf weights. In addition to this, it was found that selenium applications in salinity conditions had positive effects on dry leaf weight in sage and fresh root and fresh shoot weights in mountain tea. As a result of correlation analysis, positive correlations were found between dry leaf weight and other characteristics especially in mountain tea in terms of traits examined in both plants.


Agronomy ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 1158 ◽  
Author(s):  
Carlo Greco ◽  
Antonio Comparetti ◽  
Pierluigi Febo ◽  
Giulia La Placa ◽  
Michele Massimo Mammano ◽  
...  

The aim of this work is to assess the usefulness of biowaste deriving from Circular Bioeconomy (CBE) processes (i.e., vermicompost, compost and digestate), as growing substrates for the partial or total replacement of peat, by measuring the vegetation biometric parameters of sage (Salvia officinalis L.)—leaf area; Soil Plant Analysis Development (SPAD) value (index of chlorophyll concentration); fresh and dry weight of leaves; stem weight; root length. The results showed that vermicompost positively influenced most of above parameters (+16.7% for leaf area, +7.3% for fresh leaf weight, +6.4% for dry leaf weight, +8.5% for fresh stem weight, +0.9% for dry stem weight, +16% for root length) and, therefore, can be used as a sustainable growing substrate, alternative to peat, for the sage soilless cultivation. Yet, the results of some biometric parameters are better with peat rather than with compost (−7.2% for SPAD value, −47.3% for fresh leaf weight, −46.8% for dry leaf weight, −32.9% for fresh stem weight, −39.1% for dry stem weight, −52.4% for fresh root weight, −56.6% for dry root weight) and digestate (−30.2% for fresh leaf weight, −33.6% for dry leaf weight, −23.9% for fresh stem weight, −27% for dry stem weight, −51.8% for fresh root weight, −34.4% for dry root weight, −16% for root length). Therefore, these results are interesting for potted plants in nursery activity, while the above differences must be verified also after the transplanting of the tested plants in open field. However, the use of all the above growing substrates alternative to peat allows the sustainable valorization of food industry by-products, plant biomass, animal manure and the Organic Fraction of Municipal Solid Waste (OFMSW).


Virus Genes ◽  
2011 ◽  
Vol 42 (2) ◽  
pp. 286-296 ◽  
Author(s):  
Jamil A. Hashmi ◽  
Yusuf Zafar ◽  
Muhammad Arshad ◽  
Shahid Mansoor ◽  
Shaheen Asad

1991 ◽  
Vol 69 (12) ◽  
pp. 2603-2607 ◽  
Author(s):  
Yoav Bashan ◽  
Hanna Levanony ◽  
Reuven Or

The association between Alternaria macrospora and Alternaria alternata, responsible for the development of alternaria blight disease in cotton, was evaluated in artificially inoculated greenhouse plants and in naturally infested field plants. When greenhouse plants were inoculated with suboptimal doses of both pathogens (< 1.2 × 104 spores/mL) infection was greater than when separately inoculated by each pathogen at optimal dosage. In field-grown, naturally infected plants (Gossypium barbadense), both pathogens were found together in more than 40% of the plants. A second field-grown cotton species (Gossypium hirsutum) exhibited infection mainly by either A. alternata or both pathogens together. When both cotton species were naturally infected by both pathogens together, the number of A. alternata spores (either airborne or on the leaf surface) was greater than that of A. macrospora. We propose that A. macrospora together with A. alternata create a disease composite responsible for alternaria blight symptoms in cotton. Key words: Alternaria, cotton diseases, Gossypium barbadense, Gossypium hirsutum.


2021 ◽  
Vol 12 ◽  
Author(s):  
Denis Okello ◽  
Sungyu Yang ◽  
Richard Komakech ◽  
Yuseong Chung ◽  
Endang Rahmat ◽  
...  

The medicinal plant, Aspilia africana, has been traditionally used in several African countries to treat many diseases such as tuberculosis, cough, inflammation, malaria, osteoporosis, and diabetes. In this study, we developed a protocol for in vitro propagation of A. africana using indirect shoot organogenesis from leaf and root explants of in vitro-grown seedlings and assessed the tissues at different developmental stages. The highest callus induction (91.9 ± 2.96%) from leaf explants was in the Murashige and Skoog (MS) medium augmented with 1.0 mg/L 6-Benzylaminopurine (BAP) and 1.0 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) while from root explants, the highest callus induction (92.6 ± 2.80%) was in the same plant tissue culture medium augmented with 0.5 mg/L BAP and 1.0 mg/L 2,4-D. The best shoot regeneration capacity from leaf-derived calli (i.e., 80.0 ± 6.23% regeneration percentage and 12.0 ± 6.23 shoots per callus) was obtained in medium augmented with 1.0 mg/L BAP and 0.05 mg/L α-Naphthaleneacetic acid (NAA); the best regeneration capacity for root-derived calli (i.e., 86.7 ± 6.24% shoot regeneration percentage and 14.7 ± 1.11 shoots per callus) was obtained in the MS medium augmented with 1.0 mg/L BAP, 0.05 mg/L NAA, and 0.1 mg/L Thidiazuron (TDZ). Regenerated plantlets developed a robust root system in 1/2 MS medium augmented with 0.1 mg/L NAA and had a survival rate of 93.6% at acclimatization. The in vitro regenerated stem tissue was fully differentiated, while the young leaf tissue consisted of largely unorganized and poorly differentiated cells with large intercellular airspaces typical of in vitro leaf tissues. Our study established a protocol for the indirect regeneration of A. africana and offers a basis for its domestication, large-scale multiplication, and germplasm preservation. To the best of our knowledge, this is the first study to develop an indirect regeneration protocol for A. africana and conduct anatomical assessment through the different stages of development from callus to a fully developed plantlet.


Irriga ◽  
2020 ◽  
Vol 25 (4) ◽  
pp. 677-682
Author(s):  
Adriana Cruz de Oliveira ◽  
Antonio Flavio Batista de Araujo ◽  
Claudivan Feitosa de Lacerda ◽  
Juvenaldo Florentino Canjá ◽  
Luciana Luzia Pinho ◽  
...  

INFLUÊNCIA DA IRRIGAÇÃO SUPLEMENTAR COM ÁGUAS RESIDUÁRIAS SOBRE AS TROCAS GASOSAS FOLIARES DO ALGODOEIRO   ADRIANA CRUZ DE OLIVEIRA1; ANTONIO FLAVIO BATISTA DE ARAUJO2; CLAUDIVAN FEITOSA DE LACERDA3; JUVENALDO FLORENTINO CANJÁ4; LUCIANA LUZIA PINHO5 E NAARA IORRANA GOMES SOUSA6     1 Mestranda no Programa de Pós-Graduação em Engenharia Agrícola, Departamento de Engenharia Agrícola, Universidade Federal do Ceará-UFC, Av. Mister Hull, s/n -Pici, Centro de Ciências Agrárias, Bloco 804, CEP: 60455-760, Fortaleza-CE, Brasil. E-mail: [email protected] 2 Doutorando no Programa de Pós-Graduação em Engenharia Agrícola, Departamento de Engenharia Agrícola, Universidade Federal do Ceará-UFC, Av. Mister Hull, s/n -Pici, Centro de Ciências Agrárias, Bloco 804, CEP: 60455-760, Fortaleza-CE, Brasil. E-mail: [email protected] 3 Professor Titular do Departamento de Engenharia Agrícola, Universidade Federal do Ceará-UFC, Av. Mister Hull, s/n -Pici, Centro de Ciências Agrárias, Bloco 804, CEP: 60455-760, Fortaleza-CE, Brasil. E-mail: [email protected] 4Mestrando no Programa de Pós-Graduação em Engenharia Agrícola, Departamento de Engenharia Agrícola, Universidade Federal do Ceará-UFC, Av. Mister Hull, s/n -Pici, Centro de Ciências Agrárias, Bloco 804, CEP: 60455-760, Fortaleza-CE, Brasil. E-mail: [email protected] 5 Mestranda no Programa de Pós-Graduação em Engenharia Agrícola, Departamento de Engenharia Agrícola, Universidade Federal do Ceará-UFC, Av. Mister Hull, s/n -Pici, Centro de Ciências Agrárias, Bloco 804, CEP: 60455-760, Fortaleza-CE, Brasil. E-mail: [email protected] 6 Mestranda no Programa de Pós-Graduação em Engenharia Agrícola, Departamento de Engenharia Agrícola, Universidade Federal do Ceará-UFC, Av. Mister Hull, s/n -Pici, Centro de Ciências Agrárias, Bloco 804, CEP: 60455-760, Fortaleza-CE, Brasil. E-mail: [email protected]     1 RESUMO   O uso de águas residuárias na irrigação tem crescido como suplementação para driblar a escassez hídrica. Contudo, pouco se sabe sobre as respostas das culturas sobre sua aplicação. Assim, objetivou-se avaliar os impactos da irrigação suplementar com águas residuárias tratadas sobre as trocas gasosas do algodoeiro (Gossypium hirsutum L), simulando-se cenários hídricos considerados normais, seca e seca severa, na presença e ausência de NPK. O experimento foi conduzido no município de Russas – CE, em área vizinha à lagoa de estabilização da CAGECE – Companhia de Água e Esgoto do Estado do Ceará, em DBC, no esquema de parcelas subsubdivididas, com quatro blocos. As parcelas foram cenários hídricos (normal, seca e seca severa); a subparcela foi a suplementação ou não com água residuária tratada nos veranicos simulados; a subsubparcela foi a aplicação ou não de NPK. A cultivar utilizada foi BR 433. Aos 25 dias após a semeadura foram analisadas fotossíntese, transpiração, concentração interna de CO2 e temperatura foliar. Os cenários de seca e seca severa apresentaram pequeno impacto sobre as trocas gasosas foliares do algodoeiro. Entretanto, a irrigação suplementar reduziu a temperatura foliar e atenuou os efeitos do cenário de seca sobre a fotossíntese, independente da aplicação ou não de NPK.   Palavras-chave: Gossypium hirsutum L, Reúso de água, Seca.         OLIVEIRA, A. C. de; ARAUJO, A. F. B. de; LACERDA, C. F. de; CANJÁ, J. F.; PINHO, L. L.; SOUSA, N. I. G. INFLUENCE OF SUPPLEMENTAL IRRIGATION WITH WASTEWATER ON COTTON LEAF GAS EXCHANGE     2 ABSTRACT   The use of wastewater in irrigation has grown as a supplement to circumvent water scarcity. However, little is known about the responses of cultures to its application. Thus, the objective was to evaluate the impacts of supplementary irrigation with treated wastewater on gas exchange of cotton (Gossypium hirsutum L), simulating water scenarios considered normal, drought and severe drought, in the presence and absence of NPK. The experiment was conducted in the municipality of Russas - CE, in an area next to the stabilization pond of CAGECE - Companhia de Agua e Esgoto do Estado do Ceará, in DBC, in the subdivided plot scheme, with four blocks. The plots were water scenarios (normal, drought and severe drought); the subplot was supplemented or not with wastewater treated in the simulated summer days; the sub-installment was the application or not of NPK. The cultivar used was BR 433. At 25 days after sowing, photosynthesis, transpiration, internal CO2 concentration and leaf temperature were analyzed. The drought and severe drought scenarios had little impact on the cotton leaf gas exchange. However, supplementary irrigation reduced leaf temperature and attenuated the effects of drought scenario on photosynthesis, whether or not NPK was applied.   Keywords: Gossypium hirsutum L, Water reuse, Drought.


2020 ◽  
Vol 11 (3) ◽  
pp. 101
Author(s):  
I G.A.A. INDRAYANI ◽  
EMY SULISTYOWATI

<p>ABSTRACT<br />Ketahanan tanaman terhadap serangga hama berdasarkan karakter<br />morfologi bulu (trichom) pada daun merupakan salah satu cara potensial<br />mengurangi penggunaan insektisida kimia dalam pengendalian hama.<br />Serangga hama pengisap Bemisia tabaci pada tanaman kapas juga dapat<br />dikendalikan dengan menggunakan varietas kapas resisten berdasarkan<br />karakter morfologi bulu daun. Penelitian peranan kerapatan bulu daun<br />pada tanaman kapas terhadap kolonisasi B. tabaci Gennadius dilakukan di<br />Kebun Percobaan Pasirian, Kabupaten Lumajang, dan di Laboratorium<br />Entomologi Balai Penelitian Tanaman Tembakau dan Serat Malang, mulai<br />April hingga Juli 2005. Tujuan penelitian adalah untuk mengetahui<br />peranan kerapatan bulu daun pada beberapa aksesi plasma nutfah kapas<br />terhadap kolonisasi B. tabaci. Perlakuan terdiri atas 11 aksesi plasma<br />nutfah kapas yang dipilih berdasarkan penilaian visual pada karakter<br />kerapatan bulu daun yang mewakili kerapatan bulu rendah hingga tinggi,<br />yaitu: (1) KK-3 (KI 638), (2) Kanesia 1 (KI 436), (3) A/35 Reba P 279 (KI<br />257), (4) Acala 1517 (KI 174), (5) Asembagus 5/A/1 (KI 162), (6) 619-<br />998xLGS-10-77-3-1 (KI 76), (7) DP Acala 90 (KI 23), (8) TAMCOT SP<br />21 (KI 6)), (9) Kanesia 8 (KI 677), (10) CTX-8 (KI 494), dan (11) CTX-1<br />(KI 487). Penelitian disusun dalam rancangan acak lengkap (RAL) dengan<br />10 ulangan. Paramater yang diamati adalah jumlah bulu daun, telur dan<br />nimfa pada 1 cm2 luas daun, serta jumlah imago B. tabaci pada daun<br />ketiga dari atas tanaman. Hasil penelitian menunjukkan bahwa kerapatan<br />bulu daun berkorelasi positif dengan kolonisasi B. tabaci (R=0,9701).<br />Semakin tinggi kerapatan bulu daun, semakin meningkat kolonisasi B.<br />tabaci. Kolonisasi B. tabaci lebih tinggi pada CTX-1, CTX-8, Kanesia 8,<br />dan KK-3 (150-250 individu/cm 2 luas daun) karena tingkat kerapatan bulu<br />daun juga lebih tinggi (150-300 helai/cm 2 luas daun) dibanding TAMCOT<br />SP 21, DP Acala 90, 619-998xLGS-10-77-3-1, Asembagus 5/A/1, Acala<br />1517, A/35 Reba P 279, dan Kanesia 1 yang memiliki kerapatan bulu daun<br />(0-100 helai/cm 2 luas daun) dan tingkat kolonisasi B. tabaci (&lt;100<br />individu/cm 2 luas daun) lebih rendah.<br />Kata kunci : Kapas, Gossypium hirsutum, plasma nutfah, hama, Bemisia<br />tabaci, trichom, kolonisasi, Jawa Timur</p><p><br />ABSTRACT<br />Role of trichome density of cotton leaf to colonization of<br />Bemisia tabaci Gennadius<br />Trichome-based host plant resistance offers the potential to reduce<br />chemical insecticides used in insect pest control. Cotton whitefly, Bemisia<br />tabaci can be controlled by using resistant variety based on trichome<br />density as plant morphological characteristics. The study on the role of<br />trichome density of cotton accessions on the colonization of B. tabaci was<br />carried out at Pasirian Experimental Station at Lumajang, and at<br />Entomology Laboratory of Indonesian Tobacco and Fiber Crops Research<br />Institute (IToFCRI ) in Malang from April to July 2005. Treatments<br />included 11 cotton accessions, viz. (1) KK-3 (KI 638), (2) Kanesia 1 (KI<br />436), (3) A/35 Reba P 279 (KI 257), (4) Acala 1517 (KI 174), (5)<br />Asembagus 5/A/1 (KI 162), (6) 619-998xLGS-10-77-3-1 (KI 76), (7) DP<br />Acala 90 (KI 23), (8) TAMCOT SP 21 (KI 6)), (9) Kanesia 8 (KI 677),<br />(10) CTX-8 (KI 494), and (11) CTX-1 (KI 487). The experiment was<br />arranged in completely randomized design with ten replications.<br />Parameters observed were trichome density, number of eggs and nymphs<br />on one cm2 of leaf and adult of B. tabaci on 3rd highest leaf of cotton<br />plant. The result showed that trichome density was positively correlated<br />with B. tabaci colonization (R=0,9701) in which higher trichome density<br />of cotton leaf has resulted in great colonization of B. tabaci. Bemisia<br />tabaci colonisation was higher on CTX-1, CTX-8, Kanesia 8, and KK-3<br />(150-250 individu/cm2 of leaf) due to dense trichome (150-300<br />trichomes/cm2 leaf) as compared with other accessions, viz. TAMCOT<br />SP 21, DP Acala 90, 619-998xLGS-10-77-3-1, Asembagus 5/A/1, Acala<br />1517, A/35 Reba P 279, and Kanesia 1 which showed less density of leaf<br />trichome (0-100 trichomes/cm2 of leaf) and B. tabaci colonization (&lt; 100<br />individu/cm2 of leaf).<br />Key words : Cotton, Gossypium hirsutum, cotton accession, pest,<br />Bemisia tabaci, trichome, colonization</p>


Weed Science ◽  
1968 ◽  
Vol 16 (4) ◽  
pp. 481-484 ◽  
Author(s):  
C. R. Swanson ◽  
H. R. Swanson

Simultaneous treatment with certain carbamate insecticides inhibited degradation of 3-(p-chlorophenyl)-1,1-dimethyrurea (monuron) in cotton (Gossypium hirsutumL.) leaf discs. Monuron degradation was strongly inhibited by 1-naphthyl methylcarbamate (carbaryl) and 4-benzothiophene-N-methylcarbamate, but 2,3-dihydro-2,2-dimethyl-7-benzofuranyl methylcarbamate was ineffective. Effective carbamates did not prevent the metabolism of monuron to 1-(p-chlorophenyl)-3-methylurea (hereinafter referred to as monomethylmonuron), but they inhibited subsequent degradation of monomethylmonuron.


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