High-Speed Optical Resolution Photoacoustic Microscopy With a MEMS Scanner: Novel and Simple Distortion Correction Method

Abstract Optical resolution photoacoustic microscopy (OR-PAM) is a remarkable biomedical imaging tool that can selectively visualize microtissues with optical-dependent high resolution. However, traditional OR-PAM using mechanical stages provides slow imaging speed, making biological interpretation of in-vivo tissue difficult. Here, we developed a high-speed OR-PAM using a recently commercialized MEMS mirror. This system (MEMS-OR-PAM) consisted of a 1-axis MEMS mirror and a mechanical stage. Furthermore, this study proposed a novel calibration method that quickly removes the spatial distortion caused by fast MEMS scanning. The proposed calibration method needs to run imaging sequence only once using a ruler target and it can easily correct distortions caused by both the scan geometry of the MEMS mirror and its nonlinear motion. The combination of the MEMS-OR-PAM and the distortion correction method was verified by three experiments.; 1) Leaf skeleton phantom imaging to test the distortion correction efficacy.; 2) Spatial resolution and depth of focus (DOF) measurement for the system performance.; 3) In-Vivo finger capillaries imaging to verify their biomedical use. The results showed that the combination could achieve a high-speed (32 sec in 2 mm×4 mm) and high-lateral resolution (~6 µm) imaging capability and precisely visualize the circulating structure of the finger capillaries.

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In vivo functional chronic imaging of a small animal model using optical-resolution photoacoustic microscopy

Medical Physics ◽

10.1118/1.3137572 ◽

2009 ◽

Vol 36 (6Part1) ◽

pp. 2320-2323 ◽

Cited By ~ 42

Author(s):

Song Hu ◽

Konstantin Maslov ◽

Lihong V. Wang

Keyword(s):

Animal Model ◽

Optical Resolution ◽

Small Animal ◽

Photoacoustic Microscopy ◽

Small Animal Model

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High-speed high-resolution laser diode-based photoacoustic microscopy for in vivo microvasculature imaging

Visual Computing for Industry Biomedicine and Art ◽

10.1186/s42492-020-00067-5 ◽

2021 ◽

Vol 4 (1) ◽

Author(s):

Xiufeng Li ◽

Victor T C Tsang ◽

Lei Kang ◽

Yan Zhang ◽

Terence T W Wong

Keyword(s):

High Resolution ◽

High Speed ◽

High Performance ◽

Continuous Wave ◽

Signal To Noise Ratio ◽

Cost Effective ◽

High Signal ◽

Photoacoustic Microscopy ◽

Mouse Ear

AbstractLaser diodes (LDs) have been considered as cost-effective and compact excitation sources to overcome the requirement of costly and bulky pulsed laser sources that are commonly used in photoacoustic microscopy (PAM). However, the spatial resolution and/or imaging speed of previously reported LD-based PAM systems have not been optimized simultaneously. In this paper, we developed a high-speed and high-resolution LD-based PAM system using a continuous wave LD, operating at a pulsed mode, with a repetition rate of 30 kHz, as an excitation source. A hybrid scanning mechanism that synchronizes a one-dimensional galvanometer mirror and a two-dimensional motorized stage is applied to achieve a fast imaging capability without signal averaging due to the high signal-to-noise ratio. By optimizing the optical system, a high lateral resolution of 4.8 μm has been achieved. In vivo microvasculature imaging of a mouse ear has been demonstrated to show the high performance of our LD-based PAM system.

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In vivo three dimensional cuticle intact Drosophila brain imaging using laser scanning optical resolution photoacoustic microscopy

Photons Plus Ultrasound: Imaging and Sensing 2019 ◽

10.1117/12.2508091 ◽

2019 ◽

Author(s):

Kai-Yao Chang ◽

Hsuan Ou-Yang ◽

Pei-Shan Ho ◽

Shun-Chi Wu ◽

Yen-Yin Lin ◽

...

Keyword(s):

Brain Imaging ◽

Laser Scanning ◽

Three Dimensional ◽

Optical Resolution ◽

Photoacoustic Microscopy ◽

Drosophila Brain

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Deep-learning-based motion-correction algorithm in optical resolution photoacoustic microscopy

Visual Computing for Industry Biomedicine and Art ◽

10.1186/s42492-019-0022-9 ◽

2019 ◽

Vol 2 (1) ◽

Cited By ~ 2

Author(s):

Xingxing Chen ◽

Weizhi Qi ◽

Lei Xi

Keyword(s):

Deep Learning ◽

Optical Resolution ◽

Motion Artifacts ◽

Photoacoustic Microscopy ◽

Simulation Studies ◽

Correction Algorithm ◽

Brain Vessels ◽

Capillary Networks ◽

Motion Corrections

Abstract In this study, we propose a deep-learning-based method to correct motion artifacts in optical resolution photoacoustic microscopy (OR-PAM). The method is a convolutional neural network that establishes an end-to-end map from input raw data with motion artifacts to output corrected images. First, we performed simulation studies to evaluate the feasibility and effectiveness of the proposed method. Second, we employed this method to process images of rat brain vessels with multiple motion artifacts to evaluate its performance for in vivo applications. The results demonstrate that this method works well for both large blood vessels and capillary networks. In comparison with traditional methods, the proposed method in this study can be easily modified to satisfy different scenarios of motion corrections in OR-PAM by revising the training sets.

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Dual-Polarized Fiber Laser Sensor for Photoacoustic Microscopy

Sensors ◽

10.3390/s19214632 ◽

2019 ◽

Vol 19 (21) ◽

pp. 4632 ◽

Cited By ~ 1

Author(s):

Lin ◽

Liang ◽

Jin ◽

Wang

Keyword(s):

Fiber Laser ◽

Optical Resolution ◽

High Sensitivity ◽

Low Noise ◽

Laser Sensor ◽

Photoacoustic Microscopy ◽

Label Free ◽

Laser Sensors ◽

Dual Polarized

Optical resolution photoacoustic microscopy (OR-PAM) provides high-resolution, label-free and non-invasive functional imaging for broad biomedical applications. Dual-polarized fiber laser sensors have high sensitivity, low noise, a miniature size, and excellent stability; thus, they have been used in acoustic detection in OR-PAM. Here, we review recent progress in fiber-laser-based ultrasound sensors for photoacoustic microscopy, especially the dual-polarized fiber laser sensor with high sensitivity. The principle, characterization and sensitivity optimization of this type of sensor are presented. In vivo experiments demonstrate its excellent performance in the detection of photoacoustic (PA) signals in OR-PAM. This review summarizes representative applications of fiber laser sensors in OR-PAM and discusses their further improvements.

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