scholarly journals Determination of Genetic Diversity in European Cranberrybush(Viburnum opulus L.) Genotypes Based on Morphological, Phytochemical and ISSR Markers

2021 ◽  
Author(s):  
mehmet yaman
Keyword(s):  
Genetic Diversity ◽  
Similarity Index ◽  
Issr Markers ◽  
Leaf Length ◽  
Total Phenolic ◽  
Viburnum Opulus ◽  
Important Place ◽  
Total Anthocyanin ◽  
Fruit Species ◽  
Polymorphism Rate

Abstract Turkey's plant diversity varies considerably. Many of these plants are native and commercially grown. European Cranberrybush, which is among the fruit species grown economically in the country, is also of interest in terms of health. In this study, it was aimed to determine genetic diversity with morphological, molecular, and phytochemical markers in 24 different genotypes from Kayseri province, which has an important place in the production of European Cranberrybush in Turkey. The results show that wide variations were detected between genotypes in the morphological parameters. While the genotype G13 was the prominent genotype compared to other genotypes in leaf length (130.69 mm), leaf width (135.76 mm) and fruit length (10.01 mm), the range in fruit weights of genotypes varied between 0.16 g and 0.80 g. In ISSR marker analysis, a total of 73 scoreable bands were obtained from 11 different primers, and 44 of these bands were polymorphic bands. The average polymorphism rate in the study was 60.27%, and the similarity index of the genotypes varied between 0.77 and 0.95. Total flavonoid, total phenolic and total anthocyanin contents ranged from 106.28 mg CAE/100 g to 318.87 mg CAE/100 g, 451.23 mg GAE/100 g to 679.57 mg GAE/100 g, 21.36 mg cyn-3-gluc /100 g to 16.48 mg cyn-3-gluc /100 g, respectively. It is thought that the results of the study may be useful to plant breeders in terms of its development and preservation, as well as giving an opinion to the researchers in new studies to be carried out in the European Cranberry.

scholarly journals Elucidation of Genetic Diversity as Morphological, Phytochemical and Molecular Markers in Some Hawthorn (Crataegus) Genotypes

2021 ◽  
Author(s):  
Ercan YILDIZ ◽  
Mehmet YAMAN ◽  
Ahmet SÜMBÜL ◽  
Ahmet SAY
Keyword(s):  
Genetic Diversity ◽  
Principal Component Analysis ◽  
Molecular Marker ◽  
Geographical Location ◽  
Similarity Index ◽  
Principal Component ◽  
Component Analysis ◽  
Biochemical Properties ◽  
Total Phenolic ◽  
Fruit Species

Abstract Backround: Thanks to its ecological and geographical location, Turkey is the homeland of many fruit species and allows many fruit species to be grown. Hawthorn, which is understood to be important in human health and nutrition, is one of these fruit types. This study was carried out to identify morphological, biochemical and molecular genetic variations of 22 hawthorn genotypes belonging to three different species collected from Kayseri province. Methods and Results: Morphological, biochemical and molecular marker (ISSR) techniques were used to determine genetic diversity. The fruit and leaf characteristics of the genotypes showed differences. Among the biochemical properties of the genotypes, the antioxidant activity ranged from 23.13–61.59%, the total flavonoid content ranged from 16.63 to 57.22 mg QE/100 g, and the total phenolic content ranged from 277.28 to 310.80 mg GAE/100 g. Genotypes were compared with principal component analysis according to their morphological and biochemical characteristics. In the principal component analysis, species generally formed similar clusters. In molecular marker analysis, 101 bands were obtained from 13 ISSR primers. 76 of the bands are polymorphic and the polymorphism rate was calculated as 75.24%. The similarity index in the UPGMA dendogram obtained as a result of the molecular analysis ranged between 0.71 and 0.88. In the dendrogram, genotypes did not show a dense clustering by species. Conclusion The results obtained may benefit researchers in the determination and protection of gene sources in breeding studies on hawthorn species.

scholarly journals Evaluation of European Cranberrybush (Viburnum opulus L.) genotypes for agro-morphological, biochemical and bioactive characteristics in Turkey

2017 ◽  
Vol 29 (2) ◽  
pp. 181-188 ◽  
Author(s):  
Nilda Ersoy ◽  
Sezai Ercisli ◽  
Muttalip Gundogdu
Keyword(s):  
Vitamin C ◽  
Antioxidant Capacity ◽  
Fruit Weight ◽  
Soluble Solids ◽  
Total Phenolic ◽  
Viburnum Opulus ◽  
Total Anthocyanin ◽  
Antioxidant Power ◽  
Total Anthocyanins ◽  
Ferric Reducing Antioxidant Power

AbstractThe study evaluated some agro-morphological (fruit weight, fruit flesh ratio, fruit skin colour, number of fruits per raceme, number of racemes per bush), biochemical (vitamin C, soluble solids content, organic acids), and bioactive (total phenolics, total anthocyanins, total flavonoids, and antioxidant capacity) characteristics of the fruit of a number of selected European Cranberrybush (Viburnum opulus L.) genotypes grown in the Sivas province of Turkey. To evaluate the antioxidant capacity, ferric reducing antioxidant power (FRAP) assays were performed. The results showed genotype-specific differences in most of the agro-morphological, biochemical and bioactive characteristics. The range of fruit weight, number of fruits per raceme, and number of racemes per bush was between 0.67 and 0.82 g, 40.7 and 57.1, and 470 and 581, respectively. The highest vitamin C and soluble solids contents were found in the fruits of genotypes SIV-9 and SIV-6 as 39 mg per 100 g and 12.6%, respectively. Tartaric acid was the main organic acid in all the genotypes; it ranged from 120 to 144 mg per 100 g of fruit FW. Total phenolic, total anthocyanin and total flavonoid contents ranged from 621 to 987 mg gallic acid equivalents per 100 g, 15 to 51 mg cyanidin-3-rutinoside equivalents per 100 g, and 202 to 318 mg rutin equivalents per 100 g, respectively. Genotype SIV-10 had the highest antioxidant capacity (34.90 μmol Trolox per g, based on FRAP assays). The present study shows the potential of the evaluated European Cranberrybush genotypes for improving the nutritional value through germplasm enhancement programmes.

Genetic Relationship in Sainfoin (Onobrychis viciifolia) Landraces Cultivated East Anatolia by using RAPD and ISSR Markers

2021 ◽  
Author(s):  
Mehmet Macit Ertuş
Keyword(s):  
Genetic Diversity ◽  
Diversity Index ◽  
Calcareous Soils ◽  
Similarity Index ◽  
Issr Markers ◽  
High Genetic Diversity ◽  
Onobrychis Viciifolia ◽  
Young Leaves ◽  
Rapd And Issr ◽  
Issr Primers

Background: Sainfoin (Onobrychis viciifolia) is a forage crop that yields high in arid and calcareous soils and is cultivated in large areas. There aren’t many genetic diversity studies on the varieties of cultured sainfoin. This study was conducted to determine the genetic diversity and the degree of relationship between 23 cultivated landraces and one registered variety. Methods: To take samples from the populations, seeds were sown in the field in 2014. Samples were taken from the young leaves of the plants and preserved at -80oC in same year. RAPD and ISSR primers were used in the study. The bands obtained as a result of PCR were recorded and the data of both methods were also evaluated by combining them. Result: In the study, 5 RAPD and 4 ISSR primers were used and a total of 49 bands were obtained. Of 29 bands obtained using RAPD primers, 20 were found to be polymorphic and of 20 bands obtained using ISSR primers, 15 were found to be polymorphic. It was found that there was a very low correlation between the two methods. Using RAPD and ISSR markers and RAPD + ISSR combination, the similarity index among populations was found to be between 0.25-0.95, 0.5-1.00 and 0.45-0.91, respectively. The Nei’s genetic diversity index was found to be between 0.3365, 0.2656 and 0.3018 with RAPD, ISSR primers and RAPD + ISSR combination, respectively. Based on the dendrograms obtained using RAPD, ISSR primers and RAPD + ISSR combination, the populations under analysis were classified into 3, 3 and 5 groups, respectively. With this study, the closest populations were identified and a significantly high genetic diversity was detected.

scholarly journals Genetic Diversity of Remaining Populations of Mangaba (Hancornia speciosa Gomes) in Restingas of Brazil

2017 ◽  
Vol 9 (2) ◽  
pp. 46 ◽  
Author(s):  
Ana Veruska Cruz da Silva ◽  
Julie Anne Espíndola Amorim ◽  
Marília Freitas de Vasconcelos Melo ◽  
Ana Da Silva Ledo ◽  
Allivia Rouse Carregosa Rabbani
Keyword(s):  
Genetic Diversity ◽  
Genetic Relationships ◽  
Issr Markers ◽  
Shannon Index ◽  
Conservation Strategies ◽  
Principal Coordinates Analysis ◽  
Fruit Species ◽  
Principal Coordinates ◽  
Hancornia Speciosa ◽  
Brazilian Northeast

Mangaba (Hancornia speciosa Gomes) is a fruit species that is native to Brazil, and has social, economic and cultural importance. Knowledge of the genetic relationships between the remaining populations is essential in order to promote conservation strategies for these genetic resources. In the present study, it was evaluated the genetic diversity of 35 individuals from three remaining restingas areas in the states of Ceará (Iguape and Cascavel) and Pernambuco (Tamandaré), located in the Brazilian Northeast. Nine ISSR primers were used to determine the genetic variability. Sixty-one fully polymorphic fragments (100%) were generated. The largest (10) and smallest (5) number of fragments were obtained with the primers HB14 and HB12, respectively. The Shannon index (I = 0.40), the genetic diversity (H = 0.30), and the percentage of polymorphic loci (%P = 73.77%) were also estimated. Both the methods of UPGMA and the Principal Coordinates Analysis (PCoA) clustered individuals according to their place of origin. Genetic divergence was greater within population (64%) than between them (36%). This may indicate a strong genetic structure, i.e., the gene flow rate between populations is low, favoring inbreeding. ISSR markers were efficient for the analysis of genetic diversity, for the identification of clusters, and for the estimation of the genetic distance between and within populations.

scholarly journals Determination of genetic diversity among some almond accessions

Genetika ◽  
2015 ◽  
Vol 47 (1) ◽  
pp. 13-22 ◽  
Author(s):  
Hasan Pinar ◽  
Sezai Ercisli ◽  
Mustafa Unlu ◽  
Mustafa Bircan ◽  
Aydın Uzun ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Cultivar Identification ◽  
Genetic Relationships ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
Geographical Isolation ◽  
Fruit Species ◽  
Rapd And Issr ◽  
Simple Sequence

More recently the use of different molecular markers in fruit species to determine particularly genetic diversity, genetic relationships and cultivar identification has been gained more importance. In the study, 13 randomly amplified polimorfic DNA (RAPD) and 4 inter-simple sequence repeat (ISSR) markers were used to evaluate genetic relationships among 95 almong accessions (26 foreign cultivars and 69 national cultivars and selections). The all plant material found in Almond Germplasm Repository in Gaziantep, Turkey. Both RAPD and ISSR markers distinguished the almond cultivars and selections in various levels. 17 RAPD and ISSR markers yielded a total of 73 scorable bands, which 51 are polymorphic. The two marker system exhibited variation with regard to average band sizes and polymorphism ratio. The average polymorphism was higher in ISSR (88%) compared to RAPD (74%). RAPD and ISSR marker systems were found to be useful for determining genetic diversity among almong genotypes and cultivars. Combining of two dendrograms obtained through these markers show different clustering of 96 almond specimens without geographical isolation. These results supported that almonds in Turkey indicated considerable genetic diversity.

scholarly journals Physic nut germplasm genetic diversity identified by internal simple sequence repeats (ISSR) markers

2017 ◽  
Vol 2 (2) ◽  
pp. 48
Author(s):  
Stella Maris Nucci ◽  
Maria Imaculada Zucchi ◽  
Glyn Mara Figueira ◽  
Renata Silva-Mann ◽  
José Baldin Pinheiro
Keyword(s):  
Genetic Diversity ◽  
Bayesian Analysis ◽  
Similarity Index ◽  
Production Capacity ◽  
Germplasm Collection ◽  
Issr Markers ◽  
Physic Nut ◽  
Jaccard Similarity ◽  
Breeding Programs ◽  
Simple Sequence

The physic nut has attracted great interest for their socioeconomic promising vegetable oil production capacity that can be used in biodiesel manufacturing. However, the species is still under a domestication process and many studies are still needed to start a breeding program. In this context, this study aimed to evaluate the genetic diversity in 29 physic nut accessions from two germplasm banks using 14 ISSR markers. Genetic characterization revealed that 86.64% of the genetic variation is present within the groups, whereas 13.36% corresponded to the variation between the groups. The total mean number of alleles per locus was 1.99 and the effective number of alleles corresponded to 1.42. The Bayesian analysis allocated the access into four clusters, showing that the clusters formed showed no relation to the origin of access. The Jaccard similarity index indicated that the extent of similarity between accessions ranged from 0.23 to 0.94. The dendrogram formed the same four access groups as those formed by the Bayesian analysis, and all groups were in agreement in both analyses. The study demonstrated the need and importance of a germplasm collection from several regions and countries in order to preserve the genetic diversity of the species and the development of Euphorbiaceae breeding programs

scholarly journals INTER-SIMPLE SEQUENCE REPEAT ANALYSIS AND ALLELIC DIVERSITY AMONG GENOTYPES OF CULTIVATED Digitaria species

2018 ◽  
Vol 30 (2) ◽  
pp. 01-08
Author(s):  
D. A. Animasaun ◽  
K. F. Awujoola ◽  
R. Krishnamurthy ◽  
J. A. Morakinyo
Keyword(s):  
Genetic Diversity ◽  
Simple Sequence Repeat ◽  
Similarity Index ◽  
Allelic Diversity ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
Sequence Repeat ◽  
Simple Sequence Repeat Analysis ◽  
First Time ◽  
Simple Sequence

Acha (Digitaria exilis Kipps. and D. iburua Stapf.) are valuable indigenous food crops in West Africa, but despite several economic potentials of this crop, little or no attention is paid to its germplasm evaluation and improvement. In this study, we assessed genetic diversity and relationship among genotypes of cultivated acha in Nigeria using Inter-Simple Sequence Repeat (ISSR) markers for the first time. Genomic DNA were extracted from the genotypes and we performed fragment amplification by Polymerase Chain Reaction (PCR). A total of 95 loci consisting of 790 bands were amplified by 13 ISSR primers, out of which 53.37% were polymorphic. Loci amplification per primer ranged from 5-10, with an average of 7.30 loci per primer. Eight of the primers had above 50% polymorphism. Cluster analysis separated the genotypes into two major groups; a group consisting of two D. exilis genotypes and the other comprising a mixture of genotypes. The D. exilis in the latter group were distant members and was only similar at 0.72 similarity index. The polymorphism we obtained in the present study showed that the ISSR markers are effective for assessment of genetic diversity of the genotypes. Clustering of D. exilis and D. iburua together suggests a common progenitor but could have been separated by geographical isolation mechanism

scholarly journals Genetic variability of lemon basil (Ocimum × africanum Lour.) from Indonesian archipelago based on morphological and inter-simple sequence repeats markers

2020 ◽  
Vol 21 (12) ◽  
Author(s):  
Kurnia Makmur ◽  
TATIK CHIKMAWATI ◽  
SOBIR SOBIR
Keyword(s):  
Genetic Diversity ◽  
Genetic Variability ◽  
Similarity Index ◽  
Morphological Characters ◽  
Molecular Data ◽  
Issr Markers ◽  
Morphological Observation ◽  
Indonesian Archipelago ◽  
Morphological And Molecular Data ◽  
Issr Primers

Abstract. Makmur K, Chikmawati T, Sobir. 2020. Genetic variability of lemon basil (Ocimum × africanum Lour.) from Indonesia based on morphological characters and ISSR markers. Biodiversitas 21: 5948-5953. Lemon basil (Ocimum × africanum Lour.) or kemangi is a highly beneficial plant, yet still underutilized. Therefore, providing necessary information on genetic diversity of this species is essential for further utilization. The aim of this study was to elucidate genetic diversity of 33 accessions of O. × africanum collected from four Islands of Indonesia along with three accessions of Ocimum basilicum L. Morphological observation was conducted on 37 morphological characters following the International Union for the Protection of New Varieties of Plants descriptor guidelines; subsequently, 13 ISSR primers were employed in molecular analysis. Both morphological and molecular data were analyzed based on simple matching similarity index using UPGMA method. Morphologically, O. × africanum and O. basilicum were clearly separated at the similarity index of 0.52, and among two species were divided into two groups according to two either character. ISSR analysis using 13 ISSR primers produced 111 DNA bands, and 108 of them (97.29%) were polymorphic. Cluster analysis based on ISSR data could not explicitly separate O. × africanum and O. basilicum accessions. Besides, Ocimum accessions collected from the same area did not always cluster into one group.

scholarly journals LEAF AND FRUIT CHARACTERISTICS AND GENETIC DIVERSITY OF WILD FRUIT Cerasus prostrata GENOTYPES COLLECTED FROM THE CENTRAL ANATOLIA, TURKEY

2021 ◽  
Vol 20 (2) ◽  
pp. 53-62
Author(s):  
Aydın Uzun ◽  
Mehmet Yaman ◽  
Hasan Pinar ◽  
Batuhan Durmuş Gök ◽  
İsa Gazel
Keyword(s):  
Genetic Diversity ◽  
Leaf Length ◽  
Central Anatolia ◽  
Fruit Characteristics ◽  
Significant Information ◽  
Leaf Characteristics ◽  
Fruit Species ◽  
Wild Fruit ◽  
Deciduous Fruit ◽  
Issr Primers

Cerasus prostrata (Lab.) Ser. is quite widespread in some regions of Turkey. It is a wild and deciduous fruit species. The species is commonly encountered in Central Anatolia over the foothills of Erciyes Mountain. In this study, some fruit and leaf characteristics of 30 C. prostrata genotypes collected from the foothills of Erciyes Mountain were determined and genetic diversity among them was presented. Fruit weights of the genotypes varied between 0.66–0.23 g and fruit flesh ratios varied between 84.59–63.11%. Leaf width, leaf length and petiole lengths of the genotypes respectively varied between 1.61–0.68 cm; 4.02–1.82 cm and 0.60–0.28 cm. In genetic analyses, 17 ISSR primers were used and 115 bands were obtained. Of these bands, 98 were polymorphic. All genotypes were distinguished from each other. Relatively high variation was identified between the genotypes and similarity levels varied between 0.70–0.95. Current findings revealed significant information for the preservation and appraisal of C. prostrata. Further studies are recommended for breeding and protection of this species.

Evaluation of Polyphenolic Profile and Antibacterial Activity of Pomegranate Juice in Combination with Rifampin (R) against MDR-TB Clinical Isolates

2019 ◽  
Vol 20 (4) ◽  
pp. 317-326 ◽  
Author(s):  
Manaf AlMatar ◽  
Işıl Var ◽  
Begüm Kayar ◽  
Emel Eker ◽  
Ebru Kafkas ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Phenolic Compounds ◽  
Synergistic Effect ◽  
Bacterial Growth ◽  
Total Flavonoid ◽  
Clinical Isolates ◽  
Pomegranate Juice ◽  
Total Phenolic ◽  
Total Anthocyanin ◽  
Mdr Tb

Background: The global rise of multi-drug resistant M. tuberculosis demands unconventional treatment to enhance the efficiency of current drugs. Punica granatum, which is known as pomegranate, is considered as a member of the Punicaceae family. Pomegranate, which is broadly documented for its activity against a wide spectrum of bacterial pathogens, deserves further scrutiny in this respect. Methods: Within this scope, this study investigated the effect of fresh pomegranate juice (FPJ) on the antibacterial activity of anti-tuberculosis drugs (Rifampin (R) and Isoniazid (INH)) against MDR-TB clinical isolates. The drug resistance profiles in M. tuberculosis clinical isolates were determined by susceptibility test using BACTEC MGIT 960 system. Four concentrations of fresh pomegranate juice (FPJ) (5%, 10%, 15%, and 20%) were evaluated in combination with R and INH at a dose range of (1.0 µg/ml) and (0.1 µg/ml), respectively against the MDR-TB isolates by the BACTEC MGIT 960 system. Moreover, this study scrutinized individual phenolic compounds of FPJ by using highperformance liquid chromatography (HPLC). The total polyphenols (TP), total flavonoid (TF), total anthocyanins content (TAC), and the antioxidant capacity were also assessed in FPJ. Results: Synergistic effects were observed between R and INH with FPJ against all tested strains. However, combination therapy of rifampin was more effective than isoniazid one. Therefore, the combination of R and FPJ has been used against (27) MDR-TB clinical isolates. 5% of FPJ plus R (1.0 µg/ml) were found to suppress the growth of one isolates for first group (INH and R resistant). However, 5% of FPJ demonstrated no synergistic impact with R for second (SM, R and INH resistant) and third group (INH, EMB, R and SM resistant). Moreover, 10% of FPJ and R (1.0 μg/ml) inhibited the bacterial growth of three isolates of first group and two isolates and one isolate for second and third group, respectively. Remarkably, 15% of FPJ plus R (1.0 µg/ml) appeared to inhibit the growth of MDR-TB isolates for all tested groups indicating a strong synergistic effect. Regarding H37RV, the complete inhibition of the bacterial growth was found to occur at 15% and 20% concentrations of FPJ only. Minimum inhibitory concentration (MIC) of FPJ ranged from (4% to13%) for first group and from (10% to15%) for second and third group. Thus, FPJ at 15% inhibited 100% of bacteria for all tested isolates (MIC100% =15%). Phenolic compounds identified in FPJ were gallic acid, benzoic acid, syringic, folic acid, pelargonidin, naringin+ellagic acid, naringenin, chlorogenic acid, caffeic acid, catechin, myricetin, kaempferol, quercetin, cyanidin-3-glycoside, p-cummaric acid, ferulic acid, and rutin. Total phenolic (TP), total flavonoid (TF), and total anthocyanin (TA) content were 841.5 mg/L, 638.73 mg RE/L, and 47.43 mg/L, accordingly. Conclusion: Overall, FPJ displayed synergistic effect with R against MDR-TB clinical isolates due to its high content of polyphenol and antioxidant capability.

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